Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Rat Kim1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Sample: Recombinant Kim1, Rat.)

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Kidney Injury Molecule 1 (Kim1), Polyclonal Antibody (Cat# AAA20159)

Full Name

Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1)

Reactivity

Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

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IF (Immunofluorescence) (AAA31248 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31248 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31248 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31248 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31248 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31248 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31248 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells , using PDE1C Antibody. The lane on the left was treated with blocking peptide.)

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PDE1C, Polyclonal Antibody (Cat# AAA31248)

Full Name

PDE1C Antibody

Gene Names

PDE1C; Hcam3; DFNA74; hCam-3; cam-PDE 1C

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(100%), Rabbit(88%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of PC-3 cells using anti-Calreticulin/CALR antibody (AAA19739).Overlay histogram showing PC-3 cells stained with AAA19739 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calreticulin/CALR Antibody (AAA19739, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 14. IHC analysis of Calreticulin/CALR using anti-Calreticulin/CALR antibody (AAA19739).Calreticulin/CALR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Calreticulin/CALR Antibody (AAA19739) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat skeletal muscle tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse skeletal muscle tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calreticulin/CALR antigen affinity purified polyclonal antibody (#AAA19739) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 55-65 kDa.)

WB (Western Blot) (Figure 1. Western blot analysis of Calreticulin/CALR using anti-Calreticulin/CALR antibody (AAA19739).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human COLO 320 whole cell lysates,Lane 2: human HL-60 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human SH-SY5Y whole cell lysates,Lane 6: human THP-1 whole cell lysates,Lane 7: human U251 whole cell lysates,Lane 8: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calreticulin/CALR antigen affinity purified polyclonal antibody (#AAA19739) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 55-65 kDa.)

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Calreticulin/CALR, Polyclonal Antibody (Cat# AAA19739)

Full Name

Anti-Calreticulin/CALR Antibody Picoband

Gene Names

CALR; RO; CRT; SSA; cC1qR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescent analysis of Ephrin A5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of Ephrin A5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ephrin A5 expression in Mouse heart (A), Rat heart (B), Rat kidney (C) Whole cell lysates.)

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Ephrin A5, Polyclonal Antibody (Cat# AAA27798)

Full Name

Anti-Ephrin A5 Antibody

Gene Names

EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Rabbit

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Pancreas Tissue)

WB (Western Blot) (Western Blot;Sample: Rat PlasmaPrimary Ab: 2ug/ml Rabbit Anti-Rat vWF AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

WB (Western Blot) (Western Blot:Sample: Rat Serum.)

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Von Willebrand Factor (vWF), Polyclonal Antibody (Cat# AAA20090)

Full Name

Polyclonal Antibody to Von Willebrand Factor (vWF)

Reactivity

Rat, Human, Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Mouse Uterus Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Large Intestine Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Oviduct Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse MYLK AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

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Myosin Light Chain Kinase (MYLK), Polyclonal Antibody (Cat# AAA20149)

Full Name

Polyclonal Antibody to Myosin Light Chain Kinase (MYLK)

Gene Names

Mylk; KRP; Mlck; nmMlck; smMLCK; MLCK108; MLCK210; AW489456; 9530072E15Rik; A930019C19Rik

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NGF antibody (AAA10624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure Time: 180s)

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NGF, Polyclonal Antibody (Cat# AAA10624)

Full Name

NGF Polyclonal Antibody

Gene Names

NGF; NGFB; HSAN5; Beta-NGF

Applications

Western Blot

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using CEBPA Rabbit pAb (AAA10634) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CEBPA antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CEBPA antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using CEBPA antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CEBPA antibody (AAA10634) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CEBPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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C/EBPalpha, Polyclonal Antibody (Cat# AAA10634)

Full Name

C/EBPalpha

Gene Names

CEBPA; CEBP; C/EBP-alpha

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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WB (Western Blot) (Western blot analysis of MUC2 using HT-29 whole cell lysates)

IF (Immunofluorescence) (AAA31137 staining SW480 cells by ICC/IF. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.)

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MUC2, Polyclonal Antibody (Cat# AAA31137)

Full Name

MUC2 Antibody

Gene Names

MUC2; MLP; SMUC; MUC-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)

WB (Western Blot) (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.)

Application Data (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933(2 ug/mL) and beta-actin (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

IF (Immunofluorescence) (Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.Red: NPTX2 Antibody (4573)Blue: DAPI staining)

WB (Western Blot) (Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (AAA10933) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (AAA10933) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Loading: 15 ug of lysates per lane. Antibodies: NPTX2 AAA10933 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)

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NPTX2, Polyclonal Antibody (Cat# AAA10933)

Full Name

NPTX2 Antibody

Gene Names

NPTX2; NP2; NARP; NP-II

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

NPTX2 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse leg bone using CAMP Rabbit pAb (AAA10689) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human tonsil using CAMP antibody (AAA10689) at dilution of 1:200 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using CAMP antibody (AAA10689) at dilution of 1:200 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using CAMP antibody (AAA10689) at dilution of 1:200 (40xlens). Perform mircrowave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using CAMP antibody (AAA10689) at dilution of 1:200 (40xlens) Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of THP-1 cells, using (AAA10689) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of Mouse spleen, using (AAA10689) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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CAMP, Polyclonal Antibody (Cat# AAA10689)

Full Name

CAMP Polyclonal Antibody

Gene Names

CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

WB (Western Blot) (Western BlotPositive WB detected in: U87 whole cell lysateAll lanes: ATAT1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 45, 46, 47, 38, 37, 36, 34 kDaObserved band size: 47 kDa)

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ATAT1, Polyclonal Antibody (Cat# AAA27001)

Full Name

ATAT1 Antibody

Gene Names

ATAT1; TAT; MEC17; C6orf134; Nbla00487; alpha-TAT; alpha-TAT1

Reactivity

Human

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain cells using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat brain cells using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-251MG cells using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining..)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse brain using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat brain using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of Mouse brain, using GFAP Rabbit pAb (AAA10625) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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GFAP, Polyclonal Antibody (Cat# AAA10625)

Full Name

GFAP Polyclonal Antibody

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using OLR1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using OLR1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using OLR1 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using OLR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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OLR1, Polyclonal Antibody (Cat# AAA10753)

Full Name

OLR1 Polyclonal Antibody

Gene Names

OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody (AAA28295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody (AAA28295) at dilution of 1:100 (40xlens).)

IHC (Immunohistochemistry) (Immunohistochemistry-FGD1 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody (AAA28295) at dilution of 1:100 (40xlens).)

IHC (Immunohistochemistry) (Immunohistochemistry-FGD1 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody (AAA28295) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FGD1 antibody (AAA28295) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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FGD1, Polyclonal Antibody (Cat# AAA28295)

Full Name

FGD1 Polyclonal Antibody

Gene Names

FGD1; AAS; FGDY; MRXS16; ZFYVE3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (AAA31214 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31214 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31214 at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31214 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31214 at 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31214 at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31214 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from COS, using MUM1 Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from Ramos, using MUM1 Antibody. The lane on the left was treated with blocking peptide.)

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MUM1, Polyclonal Antibody (Cat# AAA31214)

Full Name

MUM1 Antibody

Gene Names

PWWP3A; MUM1; MUM-1; EXPAND1; HSPC211

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of rat skeletal muscle cells using Fbx32/FBOX32 Rabbit pAb (A3193) at dilution of 1:50 (40xlens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse skeletal muscle cells using Fbx32/FBOX32 Rabbit pAb (A3193) at dilution of 1:50 (40xlens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Fbx32/FBOX32 Rabbit pAb (AAA10691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A431 cells using Fbx32/FBOX32 Rabbit pAb (AAA10691) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using FBXO32 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of Mouse skeletal muscle, using Fbx32/FBOX32 antibody (AAA10691) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FBXO32 antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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FBXO32, Polyclonal Antibody (Cat# AAA10691)

Full Name

FBXO32 Polyclonal Antibody

Gene Names

FBXO32; Fbx32; MAFbx

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using SLC19A1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using SLC19A1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using SLC19A1 antibody at dilution of 1:100 (40x lens).)

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SLC19A1, Polyclonal Antibody (Cat# AAA28277)

Full Name

SLC19A1 Polyclonal Antibody

Gene Names

SLC19A1; CHMD; FOLT; IFC1; REFC; RFC1

Applications

Western Blot

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (AAA31138 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA31138) and mouse anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)

WB (Western Blot) (Western blot analysis of extracts from Raji cells, using Prestin Ab. The lane on the left was treated with blocking peptide.)

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Prestin, Polyclonal Antibody (Cat# AAA31138)

Full Name

Prestin Antibody

Gene Names

SLC26A5; PRES; DFNB61

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western Blot:Sample: Mouse Heart Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ACP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;amples: Human Liver Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Mouse Cardiac Muscle Tissue Primary Ab: 20ug/ml Rabbit Anti-Human ACP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

Western Blot

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Acyl Carrier Protein, Mitochondrial (ACP), Polyclonal Antibody (Cat# AAA20313)

Full Name

Polyclonal Antibody to Acyl Carrier Protein, Mitochondrial (ACP)

Gene Names

NDUFAB1; ACP; ACP1; SDAP; FASN2A

Reactivity

Human, Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $290/0.2 mL $430/0.5 mL $520/1 mL $1,340/5 mL $2,580/2x5 mL $3,755/3x5 mL $4,865/4x5 mL $5,925/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CD47 Rabbit pAb (AAA10699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using CD47 Rabbit pAb (AAA10699) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CD47 Rabbit pAb (AAA10699) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CD47 Rabbit pAb (A1838) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CD47 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CD47 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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CD47, Polyclonal Antibody (Cat# AAA10699)

Full Name

CD47 Polyclonal Antibody

Gene Names

CD47; IAP; OA3; MER6

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.)

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POU2F3, Polyclonal Antibody (Cat# AAA10973)

Full Name

POU2F3 Antibody

Gene Names

POU2F3; PLA1; OCT11; PLA-1; Epoc-1; OCT-11; OTF-11; Skn-1a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

POU2F3 antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IF (Immunofluorescence) (Figure 11 Immunofluorescence Validation of ACE2 In Caco2 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10945 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)

IF (Immunofluorescence) (Figure 9 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with AAA10945 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 8 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with AAA10945 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot) (Figure 4 Western Blot Validation in Rat Thymus Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10945 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, AAA10945 (2 ug/mL) and beta-actin (1 ug)

IF (Immunofluorescence) (Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with AAA10945 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)

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ACE2, Polyclonal Antibody (Cat# AAA10945)

Full Name

ACE2 Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ACE2 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human. TAFI Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistchemistry))

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Tissue)

WB (Western Blot) (Western Blot: Sample: Recombinant TAFI, Human.)

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Thrombin Activatable Fibrinolysis Inhibitor (TAFI), Polyclonal Antibody (Cat# AAA20172)

Full Name

Polyclonal Antibody to Thrombin Activatable Fibrinolysis Inhibitor (TAFI)

Gene Names

CPB2; CPU; PCPB; TAFI

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $290/0.2 mL $435/0.5 mL $520/1 mL $1,345/5 mL $2,590/2x5 mL $3,770/3x5 mL $4,885/4x5 mL $5,965/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.Green: ST2 Antibody (3363)Blue: DAPI staining)

IHC (Immunohistochemistry) (Immunohistochemistry of ST2 in human lung cancer tissue with ST2 antibody at 5 μg/ml.)

IHC (Immunohistochemistry) (Immunohistochemistry of ST2 in mouse kidney tissue with ST2 antibody at 2 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of ST2 in Mouse Kidney cells with ST2 antibody at 20 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of ST2 in human liver tissue with ST2 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of ST2 in 293 cell lysate with ST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

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ST2, Polyclonal Antibody (Cat# AAA10923)

Full Name

ST2 Antibody

Gene Names

Il1rl1; T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ST2 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31182 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31182 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31182 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Human Fetal muscle, using PU.1/Spi1 Antibody. The lane on the left was treated with blocking peptide.)

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PU.1/Spi1, Polyclonal Antibody (Cat# AAA31182)

Full Name

PU.1/Spi1 Antibody

Gene Names

SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (Figure 10 KD Validation of BACE in DRG (Hyun, 2007)Decreased BACE1 expression in DRG following siRNA3 transfection. DRG neurons were transfected with 1 ug siRNA3 plasmid and incubated for 48 hours in 37°˚C. DRG neurons were stained for BACE1 us¬ing the Anti-BACE antibody (a,b) Neurons transfected with the control plas¬mid pSUPER-EGFP (green) did not display any changes in BACE1 expression (red). (c,d) DRG neurons transfected with siR¬NA3 displayed reduced BACE1 expression in the axon.)

WB (Western Blot) (Figure 9 KO Validation of BACE in MEF Cells (Jo et al., 2010)Wildtype and BACE -/- MEFs were exposed to HNE (15_M) for 2 h. BACE1 levels were examined by Western blot with anti-BACe antibodies (AAA10918).)

IF (Immunofluorescence) (Figure 8 KD Validation of BACE in Mouse Brain (Singer et al., 2005)Immunolabeling patterns of BACE1 expression and the lenti-siRNA distribution. Sections from APP transgenic mice treated with the eGFPtagged lenti siRNA vectors (green) were co-immunolabeled with an antibody against BACE1 (red) and imaged with the LSCM. All sections are from the hippocampus of treated mice. (a–c) Lenti-siBACE1-6–treated mice. Areas within the hippocampus expressing the eGFP tagged vector have reduced BACE1 immunolabeling. (d–f) Mice treated with the eGFP-tagged control lenti-siGlut4 show unchanged expression of BACE1 in the hippocampus. (g–i) Mice treated with a saline vehicle show unchanged expression of BACE1 in the hippocampus..)

IHC (Immunohistochemistry) (Figure 7 KD Validation of BACE in Mouse Brain (Singer et al., 2005)Characterization of the effects of lenti-siBACE1-6 expression in the brains of APP transgenic mice. (a–d) Anti-eGFP immunoreactivity in the hippocampus (the injection site) shows comparable and consistent expression of lenti-siRNA constructs in the dentate gyrus (dg) and stratus polymorphus (sp). (e) Anti-BACE1 immunoreactivity in the hippocampus of nontransgenic mice treated with lenti-siGlut4. (f) Reduced BACE1 immunostaining in the hippocampus of nontransgenic mice treated with lenti-siBACE1-6 vector. (g) Intense BACE1 immunoreactivity in the hippocampus of APP transgenic mice treated with lenti-siGlut4. (h) Reduced BACE1 expression in APP transgenic mice treated with lenti-siBACE1-6 vector. (i,j) Anti-BACE1 reacted with pyramidal cell bodies in the neocortex, which was not injected,)

WB (Western Blot) (Figure 6 KO and Overexpression Validation of BACE in Human and Mouse Brain and 293 Cells. (Singer et al., 2005)Western blot analysis of the BACE1 (AAA10918) antibody’s ability to recognize human and murine BACE1. The BACE1 antibody recognized both the mouse and human forms of BACE1. Lanes 1–4 are frontal cortex homogenates from human and mouse brains. Lane 1 is from a neurologically unimpaired aged human control case, lane 2 from a BACE1-deficient mouse, lane 3 from a nontransgenic mouse and lane 4 from hBACE1 transgenic mouse. Lanes 5–7 are lysates from HEK293T cells transfected with a plasmid vector expressing eGFP, mBACE1 and hBACE1, respectively.)

ICC (Immunocytochemistry) (Figure 5 Immunocytochemistry Validation of BACE in 3T3/NIH CellsImmunohistochemical analysis of 3T3/NIH cells using anti-BACE antibody (AAA10918) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Figure 4 Immunofluorescence Validation of BACE in 3T3/NIH CellsImmunofluorescent analysis of 4% paraformaldehydefixed mouse 3T3/NIH cells labeling BACE with AAA10918 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on 3T3/NIH cells.)

IHC (Immunohistochemistry) (Figure 3 Immunohistochemistry Validation of BACE in Mouse Brain Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BACE antibody (AAA10918) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4?C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: BACE (AAA10918) (1 μg/mL), BACE ProMab (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 1 Western Blot Validation of BACELoading: 15 ug of lysates per lane. Antibodies: BACE (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A-C: human brain tis)

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BACE, Polyclonal Antibody (Cat# AAA10918)

Full Name

BACE Antibody

Gene Names

BACE1; ASP2; BACE; HSPC104

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

BACE Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of THP-1 cells using TLR4 Rabbit pAb (AAA10771) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TLR4 Rabbit pAb (AAA10771) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TLR4 antibody (AAA10771) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using TLR4 antibody (AAA10771) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using TLR4 antibody (AAA10771) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of A-549 cells, using TLR4 antibody (AAA10771) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5min.)

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TLR4, Polyclonal Antibody (Cat# AAA10771)

Full Name

TLR4

Gene Names

TLR4; TOLL; CD284; TLR-4; ARMD10

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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WB (Western Blot) (Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)

WB (Western Blot) (Figure 8 Overexpression Validation of CXCR4 (Kozak et al., 2002)U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.)

FCM (Flow Cytometry) (Figure 7 KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010)Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4?/? astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).)

IHC (Immunohistchemistry) (Figure 6 Immunohistochemistry Validation of CXCR4Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1012) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Figure 5 Immunofluorescence Validation of CXCR4Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1012 at 4 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.)

WB (Western Blot) (Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane.Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Validation with CXCR4 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1012 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.)

WB (Western Blot) (Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane.Antibodies: 1012 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

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CXCR4, Polyclonal Antibody (Cat# AAA10962)

Full Name

CXCR4 Antibody

Gene Names

CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

CXCR4 Antibody is Protein A purified.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA26923 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26923 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA26923 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AAA26923 at dilution of 1:100)

WB (Western Blot) (Western blotAll lanes: FFAR3 antibody at 0.5ug/ml+Mouse kidney tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39 kDaObserved band size: 39 kDa)

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FFAR3, Polyclonal Antibody (Cat# AAA26923)

Full Name

FFAR3 Antibody

Gene Names

FFAR3; FFA3R; GPR41; GPR42

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunoflouorescence) (AAA31039 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31039 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31039 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31039 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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ADD1, Polyclonal Antibody (Cat# AAA31039)

Full Name

Phospho-ADD1 (Ser726) Antibody

Gene Names

ADD1; ADDA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane. Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Validation with CXCR4 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1009 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile Cell LinesLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP)

WB (Western Blot) (Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP)

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CXCR4, Polyclonal Antibody (Cat# AAA10951)

Full Name

CXCR4 Antibody

Gene Names

CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

CXCR4 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

IHC (Immunohistochemistry-Paraffin) (Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

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PTGS2 / COX2 / COX-2, Polyclonal Antibody (Cat# AAA12379)

Full Name

Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Human, Mouse, Rat, Bovine, Pig, Sheep

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot

Purity

Immunoaffinity purified

Pricing

$690/0.05 mL $1,325/2x0.05 mL $1,915/3x0.05 mL $2,475/4x0.05 mL $2,800/5x0.05 mL

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IF (Immunofluorescence) (AAA31175 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31175 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31175 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31175 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31175 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31175 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31175 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Mouse brain, using Nicotinic Acetylcholine Receptor alpha 7. The lane on the left was treated with blocking peptide.Observed bands: 48 kDa.)

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Nicotinic Acetylcholine Receptor alpha 7, Polyclonal Antibody (Cat# AAA31175)

Full Name

Nicotinic Acetylcholine Receptor alpha 7 Antibody

Gene Names

CHRNA7; NACHRA7; CHRNA7-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Human Breast cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human HMG1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

WB (Western Blot) (Western Blot:Sample: Recombinant HMG1, Human.)

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High Mobility Group Protein 1 (HMG1), Polyclonal Antibody (Cat# AAA20075)

Full Name

Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $290/0.2 mL $435/0.5 mL $520/1 mL $1,345/5 mL $2,590/2x5 mL $3,770/3x5 mL $4,885/4x5 mL $5,965/5x5 mL

WB (Western Blot) (Western blot analysis of EYFP recombinant protein with AAA27909 diluted at 1:5,000.)

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EYFP, Polyclonal Antibody (Cat# AAA27909)

Full Name

EYFP Rabbit Polyclonal Antibody

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Pricing

$415/0.1 mg $605/0.1 mL (AF350) $605/0.1 mL (AF405L) $605/0.1 mL (AF405M) $605/0.1 mL (AF405S) $605/0.1 mL (AF488) $605/0.1 mL (AF546) $605/0.1 mL (AF555) $605/0.1 mL (AF568) $605/0.1 mL (AF594) $605/0.1 mL (AF610) $605/0.1 mL (AF635) $605/0.1 mL (AF647) $605/0.1 mL (AF680) $605/0.1 mL (AF750) $605/0.1 mL (Biotin) $605/0.1 mL (FITC) $605/0.1 mL (HRP) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (PE) $1,415/2x0.1 mL (AP) $2,050/3x0.1 mL (AP) $2,655/4x0.1 mL (AP)

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using LGALS3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using LGALS3 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using LGALS3 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using LGALS3 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using LGALS3 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using LGALS3 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using LGALS3 Antibody at dilution of 1:200 (40x lens).)

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LGALS3, Polyclonal Antibody (Cat# AAA10670)

Full Name

LGALS3 Polyclonal Antibody

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.