Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse heart cells using RYR2 Rabbit pAb (AAA10638) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Mouse heart, using RYR2 antibody (AAA10638) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.)

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RYR2, Polyclonal Antibody (Cat# AAA10638)

Full Name

RYR2 Polyclonal Antibody

Gene Names

RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using RPS12 Polyclonal Antibody (AAA10775) at dilution of 1:400. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using RPS12 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using RPS12 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using RPS12 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using RPS12 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RPS12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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RPS12, Polyclonal Antibody (Cat# AAA10775)

Full Name

RPS12 Polyclonal Antibody

Gene Names

RPS12; S12

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

WB (Western Blot) (Western blot analysis of Lysates HeLa, using Phospho-CDK1-T14 Rabbit pAb (AAA10633) at 1:500 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37 degree C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

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CDK1-T14, Polyclonal Antibody (Cat# AAA10633)

Full Name

Phospho-CDK1-T14 Polyclonal Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Applications

Western Blot

Purity

Affinity Purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates, using STAT3 Rabbit pAb (AAA10727) at 1:2500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s)

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STAT3, Polyclonal Antibody (Cat# AAA10727)

Full Name

STAT3 Polyclonal Antibody

Gene Names

STAT3; APRF; HIES

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Figure 10 Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.)

IF (Immunofluorescence) (Figure 9 Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.)

WB (Western Blot) (Figure 8 Induced Expression Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)Effect of oxygen–glucose deprivation(OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. Western blot analysis of TACE in homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.)

WB (Western Blot) (Figure 7 Overexpression Validation of TACE in MCF-7 Cells. (McGowan et al., 2007)ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 (1131) antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells.)

WB (Western Blot) (Figure 6 KD Validation of TACE in MDA-MB-435 Cells. (McGowan et al., 2007)ADAM-17 protein expression, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies (1131).)

WB (Western Blot) (Figure 5 KD Validation of TACE in Monkey COS Cells. (Wang et al., 2006)COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody (1131). TACE expression levels were markedly reduced in TACE knockdown cell lysate.)

ICC (Immunocytochemistry) (Figure 4 Immunocytochemistry Validation of TACE in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE antibody (1131) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TACE 1131 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

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TACE, Polyclonal Antibody (Cat# AAA10912)

Full Name

TACE Antibody

Gene Names

ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

TACE Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30993 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30993 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30993 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 29 degree C)

IF (Immunofluorescence) (AAA30993 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-IGF1R (Tyr1346) expression in various lysates)

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IGF1R, Polyclonal Antibody (Cat# AAA30993)

Full Name

Phospho-IGF1R (Tyr1346) Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (AAA31187 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31187 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31187 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31187 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31187 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31187 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31187 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31187 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from A549, using Syntrophin alpha 1 Antibody. The lane on the left was treated with blocking peptide.)

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Syntrophin alpha 1, Polyclonal Antibody (Cat# AAA31187)

Full Name

Syntrophin alpha 1 Antibody

Gene Names

SNTA1; SNT1; LQT12; TACIP1; dJ1187J4.5

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Rabbit(88%), Dog(88%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Antibody (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-AIF1 / IBA1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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AIF1 / IBA1, Polyclonal Antibody (Cat# AAA12318)

Full Name

Goat Polyclonal to Human AIF1 / IBA1

Gene Names

AIF1; IBA1; IRT1; AIF-1; IRT-1

Reactivity

Human, Monkey, Mouse, Rat, Hamster, Pig

Applications

Immunohistochemistry, Western Blot

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$720/0.05 mg $1,385/2x0.05 mg $2,005/3x0.05 mg $2,595/4x0.05 mg $2,945/5x0.05 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of LANCL2 in mouse brain tissue with LANCL2 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of LANCL2 in human brain tissue lysate with LANCL2 antibody at 1 μg/ml.)

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LANCL2, Polyclonal Antibody (Cat# AAA10978)

Full Name

LANCL2 Antibody

Gene Names

LANCL2; TASP; GPR69B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31263 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31263 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31263 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells, using UBR4 Antibody. The lane on the left was treated with blocking peptide.)

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UBR4, Polyclonal Antibody (Cat# AAA31263)

Full Name

UBR4 Antibody

Gene Names

UBR4; p600; ZUBR1; RBAF600

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Figure 12 Overexpression Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, AAA10931 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.)

IHC (Immunohistochemistry) (Figure 11 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Brain Spike protein was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the brain of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistochemistry) (Figure 10 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Lung Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the lung of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistchemistry) (Figure 9 IHC Validation of SARS-CoV2 Spike in the Nasopharyngeal Swab Sample of the COVID-19 Patient Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the nasopharyngeal swab sample of the COVID-19 patient and no spike signal was observed in the sample of the COVID-19 negative patient. COVID-19 cases were confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IF (Immunofluorescence) (Figure 8 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Skin (Magro et al., 2020) C4d is highlighted green while COVID-19 spike protein detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) shows a red staining pattern; a yellow signal is discernible indicative of co-localization of C4d and viral protein within the microvasculature.)

IF (Immunofluorescence) (Figure 7 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Lung (Magro et al., 2020) SARS-CoV2 spike protein (red, panel C) detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) colocalized with C4d (green in panel d, merged in yellow). Spike protein (red, panel g) was also colocalized with C5b-9 (green in panel f&h, merged in yellow))

IHC (Immunohistchemistry) (Figure 6 IHC/IF Validation in COVID-19 Patient Sample (Nuovo et al., 2020) Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations. B. An intense signal for covid-19 spike protein tested by SARS-CoV-2 spike antibodies (AAA10931) was observed in the glandular cells. F-H. Co-expression of spike detected by spike antibodies (AAA10931, 0.2 ug/mL) and envelope proteins detected by envelope antibodies (, 2 ug/mL) of SARSCoV- 2 (F) documented localization of each protein to glandular cells (G, yellow). No signal was seen in oral swabs of positive cases (H). Both the spike and envelope protein detected by anti-spike antibodies (AAA10931) and anti-envelope antibodies (,) produced a signal in the nasopharyngeal swabs of the three cases and no signal was evident in the nasopharyngeal swabs of the seven controls.)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal wasobserved in macrophages of COVID-19 patient lung.)

IHC (Immunohistochemistry) (Figure 4 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

IF (Immunofluorescence) (Figure 3 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021) Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k-s) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k-m); pan-macrophage marker CD68 (magenta) (n-p); HLA-DR (magenta) and pDC marker CD123 (yellow) (q–s) and DAPI (blue).)

IF (Immunofluorescence) (Figure 2 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Nose and Tonsil (Singh et al., Nature Microbiology, 2021) Multi-label confocal immunofluorescence microscopy of nasal epithelium (20X-b, 63xh) and tonsil (20X-c,63X-i) from rhesus macaques infected with SARS-CoV-2 with SARS-CoV-2 spike-specific antibodies, AAA10931. (turquoise), DAPI (blue). Rabbit IgG isotype control antibody was used to stain the tissues to rule out any nonspecific staining (e, f).)

IF (Immunofluorescence) (Firure 1. Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)

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COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

Full Name

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody

Reactivity

Virus

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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WB (Western Blot) (Western blot analysis of Albumin expression in human liver tissue lysate with Albumin antibody at 0.25 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of Albumin in human liver tissue with Albumin antibody at 20 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of Albumin in mouse liver tissue with Albumin antibody at 20 ug/ml.Red: Albumin AntibodyBlue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of Albumin in mouse liver tissue with Albumin antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemistry of Albumin in human liver tissue with Albumin antibody at 2.5 ug/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of Albumin in mouse liver tissue with Albumin antibody at 5 ug/mL.)

WB (Western Blot) (Western blot analysis of Albumin in mouse liver tissue lysate with Albumin antibody at (A) 1 and (B) 2 ug/mL.)

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Albumin, Polyclonal Antibody (Cat# AAA10940)

Full Name

Albumin Antibody

Gene Names

ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Albumin Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) ( at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse heart using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:20 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using Dystrophin Rabbit pAb (AAA10720) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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DMD, Polyclonal Antibody (Cat# AAA10720)

Full Name

DMD Polyclonal Antibody

Gene Names

DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using MEGF10 antibody (AAA28229) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat brain using MEGF10 antibody (AAA28229) at dilution of 1:100. Blue DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MEGF10 antibody (AAA28229) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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MEGF10, Polyclonal Antibody (Cat# AAA28229)

Full Name

MEGF10 Polyclonal Antibody

Gene Names

MEGF10; EMARDD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (IHC Validation of Envelope in COVID-19 Patient Skin (Magro et al., 2020) Detection of SARS-CoV-2 Envelope protein in the blood vessels of COVID-19 patients that were confirmed by PCR. The staining shows Envelope protein expression (green) detected by envelope antibodies (AAA10932, 3 ug/mL) in the endothelial cytoplasms in thrombosed and normal appearing blood vessels with hematoxylin counterstain. The staining was negative in control normal skin/lung (not shown).)

IF (Immunofluorescence) (Co-expression of SARS-CoV-2 (COVID-19) Envelope and C5b-9 in Human Lung Tissue from the COVID-19 Patient Immunofluorescent l analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 2 ug/mL, red) and anti-C5b-9 antibody (green). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, followed by secondary antibodies at 1/500 and DAPI staining (blue). Co- expression was shown in yellow. (Courtesy of Dr. Nuovo Gerard J., OSU).)

IF (Immunofluorescence) (Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Envelope in Human Lung Tissue from the COVID-19 Patient Immunofluoorescent l analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, followed by a goat anti-rabbit IgG secondary antibody at 1/500 (red) and DAPI staining (blue). (Courtesy of Dr. Nuovo Gerard J., OSU).)

IF (Immunofluorescence) (IF Validation of Envelope in COVID-19 Patient Skin (Magro et al., 2020) Detection of SARS-CoV-2 Envelope protein in the skin of COVID-19 patients that were confirmed by PCR. The skin staining shows Envelope protein expression (green) detected by envelope antibodies (AAA10932, 3 ug/mL) in mononuclear cells with hematoxylin counterstain. The staining was negative in control normal skin/lung (not shown).)

IHC (Immunohistochemistry) (Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Envelope in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

ELISA (Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody, (AAA10932) (1 ug/mL). A direct ELISA was performed using antigen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 32 ng/mL to 2000ng/mL.)

IHC (Immunohistochemistry) (IHC Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 Envelope protein in nasopharyngeal swab samples of COVID-19 patients Panel F shows Envelope protein detected by envelope antibodies (AAA10932) was still evident 2 weeks after the initial swabs (signal is red with hematoxylin counterstain), though the amount of virus was much less than at the initial swab.)

IHC (Immunohistochemistry) (Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations F-H. Co-expression of spike detected by spike antibodies and envelope proteins detected by envelope antibodies (AAA10932) of SARS-CoV-2 (panel F) documented localization of each protein to glandular cells with negative squamous cells two weeks after full revoery (panel G, signal yellow). No signal was seen in oral swabs of positive cases (Panel H). Both the spike and envelope protein detected by anti-spike antibodies and anti-envelope antibodies (AAA10932) produced a signal in the nasopharyngeal swabs of the three vases and no signal was evident in the nasopharyngeal swabs of the seven controls.)

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COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA10932)

Full Name

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody

Reactivity

Virus

Applications

Immunofluorescence, Immunohistochemistry

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human mammary cancer using TFF1 antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of Mouse stomach, using TFF1/pS2 antibody (AAA10666) at 1:710 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.Exposure time: 90s)

WB (Western Blot) (Western blot analysis of various lysates, using TFF1/pS2 antibody (AAA10666) at 1:710 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of extracts of BT-474 cells, using TFF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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TFF1, Polyclonal Antibody (Cat# AAA10666)

Full Name

TFF1 Polyclonal Antibody

Gene Names

TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using GNA15 antibody (AAA10668) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using GNA15 antibody (AAA10668) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse heart, using GNA15 antibody.(AAA10668)Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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GNA15, Polyclonal Antibody (Cat# AAA10668)

Full Name

GNA15 Polyclonal Antibody

Gene Names

GNA15; GNA16

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of p38-γ/δ (Phospho-Tyr185/182) using EGF treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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p38 gamma/delta, Polyclonal Antibody (Cat# AAA31407)

Full Name

Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody

Gene Names

MAPK12; ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistochemistry) (AAA31126 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis of Hela cell lysate, using MT2A Antibody. The lane on the left is treated with the antigen-specific peptide.)

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MT2A, Polyclonal Antibody (Cat# AAA31126)

Full Name

MT2A Antibody

Gene Names

MT2A; MT2; MT-2; MT-II

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates, using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ITGAL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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ITGAL, Polyclonal Antibody (Cat# AAA10672)

Full Name

ITGAL Polyclonal Antibody

Gene Names

ITGAL; CD11A; LFA-1; LFA1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immuofluorescence) (Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10928 at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)

IF (Immunofluorescence) (Figure 8 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with AAA10928 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with AAA10928 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunfluorescence) (Figure 6 Immunofluorescence Validation of ACE2 in Human Test is Tissue Immunofluorescent analysis of 4% paraformalde hyde-fixed human test is tissue labeling ACE-2 with AAA10928 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (AAA10928) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Figure 4 Western Blot Validation in Rat Skin Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane. Antibodies: ACE2, AAA10928 (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug))

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ACE2, Polyclonal Antibody (Cat# AAA10928)

Full Name

ACE2 Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ACE2 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31005 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31005 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Human prostate tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31005 at 1/200 staining Human prostate tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-Tau (Ser262) Antibody expression in mouse brain and rat kidney tissues lysates.)

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Tau, Polyclonal Antibody (Cat# AAA31005)

Full Name

Phospho-Tau (Ser262) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Bovine Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Bovine IL1b AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

WB (Western Blot) (Western Blot;Sample: Recombinant IL1b, Bovine.)

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Interleukin 1 Beta, Polyclonal Antibody (Cat# AAA20864)

Full Name

Polyclonal Antibody to Interleukin 1 Beta (IL1b)

Reactivity

Bovine

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$250/0.1 mL $315/0.2 mL $490/0.5 mL $605/1 mL $1,590/5 mL $3,070/2x5 mL $4,470/3x5 mL $5,790/4x5 mL $7,080/5x5 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from THP-1 cells lysates (treated with TPA/LPS), using Cleaved Caspase-1 (Ala317) /P10 Antibody. The lane on the left was treated with blocking peptide.)

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Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)

Full Name

Cleaved-Caspase 1 (Ala317), p10 Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of 293T cells, using BRCA2 antibody (AAA10700) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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BRCA2, Polyclonal Antibody (Cat# AAA10700)

Full Name

BRCA2

Gene Names

BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2

Reactivity

Human , Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

WB (Western Blot) (PVRL4 Antibody (Center) (AAA28678) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PVRL4 antibody detected the PVRL4 protein (arrow).)

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PVRL4, Polyclonal Antibody (Cat# AAA28678)

Full Name

PVRL4 Antibody (Center)

Gene Names

PVRL4; LNIR; PRR4; EDSS1; nectin-4

Reactivity

Human

Applications

Western Blot, Dot Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TYR antibody (AAA10704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using TYR antibody (AAA10704) at dilution of 1:100 (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of rat skin using TYR antibody (AAA10704) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse skin using TYR antibody (AAA10704) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis ofhuman skin cancer using TYR antibody (AAA10704) at dilution of 1:100. Blue: DAPI for nuclear staining.)

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TYR, Polyclonal Antibody (Cat# AAA10704)

Full Name

TYR Polyclonal Antibody

Gene Names

TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using MT2A antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using MT2A antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using MT2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure Time: 90s.)

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MT2A, Polyclonal Antibody (Cat# AAA10681)

Full Name

MT2A Polyclonal Antibody

Gene Names

MT2A; MT2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (AAA31063 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-NF kappaB p65 (Ser281) expression in various lysates)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31063 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31063 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Human kidney cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Human kidney cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31063 at 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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NF kappaB p65, Polyclonal Antibody (Cat# AAA31063)

Full Name

Phospho-NF kappaB p65 (Ser281) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western blot analysis of MLZE expression in mouse spleen (A), rat kidney (B), rat spleen (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 58 kD))

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MLZE, Polyclonal Antibody (Cat# AAA27810)

Full Name

Anti-MLZE Antibody

Gene Names

GSDMC; MLZE

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

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IF (Immunofluorescence) (AAA31118 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA31118 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31118 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (AAA31118 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis of extracts from mouse lung, using GPR133 Ab.)

WB (Western Blot) (Western blot analysis of extracts from COS-7 cells, using GPR133 antibody.The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of extracts from COS-7 cells, using GPR133 antibody.The lane on the left is treated with the antigen-specific peptide.)

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GPR133, Polyclonal Antibody (Cat# AAA31118)

Full Name

GPR133 Antibody

Gene Names

ADGRD1; PGR25; GPR133

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)

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Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)

Full Name

Phospho-SMAD4 (T277) Antibody

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human, Mouse
Predicted Species Reactivity: Rat

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IF (Immunofluorescence) (AAA31240 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31240) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31240 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31240 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31240 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31240 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from C6 cells, using Anillin Antibody. The lane on the left was treated with blocking peptide.)

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Anillin, Polyclonal Antibody (Cat# AAA31240)

Full Name

Anillin Antibody

Gene Names

ANLN; scra; FSGS8; Scraps

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-FAK (Tyr576) Antibody expression in NIH-3T3 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA31066 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 43 degree C)

WB (Western Blot) (Western blot analysis of FAK phosphorylation expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31066 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31066 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31066 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31066 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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FAK, Polyclonal Antibody (Cat# AAA31066)

Full Name

Phospho-FAK (Tyr576) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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FCM (Flow Cytometry) (DDR1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of DDR1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of AAA28772 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of AAA28772 on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (DDR1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDR1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Anti-DDR1 Antibody (Center) at 1:500 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Western blot analysis of DDR1 (arrow) using rabbit polyclonal DDR1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR1 gene (Lane 2) (Origene Technologies).)

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DDR1, Polyclonal Antibody (Cat# AAA28772)

Full Name

DDR1 Antibody (Center)

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (FITC staining on IHC-PSimple: A549 cells.)

IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Human Liver Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Stomach Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ITGb3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

WB (Western Blot) (Western Blot:Sample: Recombinant ITGb3, Human.)

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Integrin Beta 3 (ITGb3), Polyclonal Antibody (Cat# AAA20173)

Full Name

Polyclonal Antibody to Integrin Beta 3 (ITGb3)

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat testis using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using LETMD1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using LETMD1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LETMD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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LETMD1, Polyclonal Antibody (Cat# AAA10722)

Full Name

LETMD1 Polyclonal Antibody

Gene Names

LETMD1; HCCR1; HCCR-1; HCCR-2; HCRR-2; 1110019O13Rik

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (AAA31262 staining H2O2 treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31262) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31262 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31262 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31262 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Mouse Kidney, using VGluT1 Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells, using VGluT1 Antibody. The lane on the left was treated with blocking peptide.)

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VGluT1, Polyclonal Antibody (Cat# AAA31262)

Full Name

VGluT1 Antibody

Gene Names

SLC17A7; BNPI; VGLUT1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis FAK (Phospho-Ser722) using EGF treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from HepG2, using FAK (Phospho-Ser722) Antibody.)

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FAK, Polyclonal Antibody (Cat# AAA31446)

Full Name

Phospho-FAK (Ser722) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cellsusing Fatty Acid Synthase antibody(AAA10626). Blue: DAPI for nuclear staining)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using FASN antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using FASN antibody at dilution of 1:100 (40x lens).)

IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody (AAA10626). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (AAA10626) at a dilition of 1:1000.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (AAA10626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of extracts of H460 cells, using FASN antibody at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)

Full Name

FASN Polyclonal Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: PANC Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.)

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PC1/3, Polyclonal Antibody (Cat# AAA11585)

Full Name

Anti-PC1/3 antibody

Gene Names

PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12

Reactivity

Reacts with: Human, rat; Predicted to work with: Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using AAA26913 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA26913 at dilution of 1:100)

WB (Western Blot) (Western BlotAll lanes: CTPS1 antibody at 6ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 67, 42 kDaObserved band size: 67 kDa)

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CTPS1, Polyclonal Antibody (Cat# AAA26913)

Full Name

CTPS1 Antibody

Gene Names

CTPS1; CTPS; IMD24

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Rat Intestine Tissue;Primary Ab: 20ug/ml Rabbit Anti-Rat PANX1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Rat PANX1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

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Pannexin 1 (PANX1), Polyclonal Antibody (Cat# AAA20074)

Full Name

Polyclonal Antibody to Pannexin 1 (PANX1)

Gene Names

Panx1; px1

Reactivity

Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$250/0.1 mL $315/0.2 mL $505/0.5 mL $610/1 mL $1,600/5 mL $3,085/2x5 mL $4,495/3x5 mL $5,825/4x5 mL $7,110/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells using AHR Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using AHR Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of AHR in paraffin-embedded mouse liver tissue using AHR Rabbit pAb (A1451) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of AHR in paraffin-embedded rat kidney tissue using AHR Rabbit pAb (AAA10754) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of AHR in paraffin-embedded human kidney tissue using AHR Rabbit pAb (AAA10754) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using AHR Rabbit pAb (A1451) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 µg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using AHR antibody (AAA10754) at dilution of 1:150. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using AHR Rabbit pAb (AAA10754) at dilution of 1:100 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using AHR Rabbit pAb (AAA10754) at dilution of 1:100 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human endometrium cancer using AHR Rabbit pAb (AAA10754) at dilution of 1:100 (40xlens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AHR antibody (AAA10754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)

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AHR, Polyclonal Antibody (Cat# AAA10754)

Full Name

AHR Polyclonal Antibody

Gene Names

AHR; bHLHe76

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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QC (Quality Control)

IHC (Immunohistochemistry) (IHC of Treponema pallidum on an FFPE Infected Skin TissueThis antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations.)

Dilution Information

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Treponema pallidum, Polyclonal Antibody (Cat# AAA13577)

Full Name

Treponema pallidum

Reactivity

Eubacteria

Applications

Immunohistochemistry

Pricing

$285/0.1 mL (Concentrate) $285/3 mL (RTU) $410/0.5 mL (Concentrate) $410/7 mL (RTU) $575/1 mL (Concentrate) $575/15 mL (RTU) $1,090/2x1 mL (Concentrate) $1,575/3x1 mL (Concentrate) $2,030/4x1 mL (Concentrate) $2,280/5x1 mL (Concentrate) $2,280/5x15 mL (RTU)

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IF (Immunofluorescence) (AAA31122 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31122) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (AAA31122 at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Rat muscle, using IL6 Ab. The lane on the left was treated with blocking peptide.)

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IL-6, Polyclonal Antibody (Cat# AAA31122)

Full Name

IL-6 Antibody

Gene Names

IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (AAA31134 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31134) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-PERK (Thr982) Ab.Lane 1: 293 cells(heat-shock treatment), blocked with antigen-specific peptidesLane 2: 293 cells(heat-shock treatment)Lane 3: A549 cells(serum starvation treatment).)

IHC (Immunohistochemistry) (AAA31134 at 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PERK, Polyclonal Antibody (Cat# AAA31134)

Full Name

Phospho-PERK (Thr982) Antibody

Gene Names

EIF2AK3; PEK; WRS; PERK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of alpha Tubulin (acetyl K40) in lysates of HeLa cells(TSA 1M, 18 hr)  , using alpha Tubulin (acetyl K40) Antibody(AF4351).)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells(TSA 1M, 18 hr), using Acetyl-alpha Tubulin (Lys40) Antibody.Lane1 was treated with Ac-blocking peptide.Lane2 was treated with Non-Ac-blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Acetyl-alpha Tubulin (Lys40) Antibody.Lane 1: Mouse kidney, blocked with antigen-specific peptides,Lane 2: Mouse kidney,Lane 3: Rat hesrt,Lane 4: K562 cells,Lane 5: Rat liver.)

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alpha Tubulin, Polyclonal Antibody (Cat# AAA31344)

Full Name

Acetyl-alpha Tubulin (Lys40) Antibody

Gene Names

TUBA1B; K-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using DiMethyl-Histone H3-K4 Rabbit pAb (AAA10651) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).)

DB (Dot Blot) (Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K4 antibody (AAA10651) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (AAA10651) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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H3K4me2, Polyclonal Antibody (Cat# AAA10651)

Full Name

Histone H3K4me2 Polyclonal Antibody

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.