Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using ACVR1C antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ACVR1C antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ACVR1C antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ACVR1C antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using ACVR1C antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ACVR1C antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of SH-SY5Y cells, using ACVR1C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Compare (5 max)

ACVR1C, Polyclonal Antibody (Cat# AAA10695)

Full Name

ACVR1C Polyclonal Antibody

Gene Names

ACVR1C; ALK7; ACVRLK7

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-p73 (Tyr99) expression in Mouse lung lysate)

WB (Western Blot) (Western blot analysis of p73 phosphorylation expression in Pervanadate treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistchemistry) (AAA30950 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30950 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30950 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

p73, Polyclonal Antibody (Cat# AAA30950)

Full Name

Phospho-p73 (Tyr99) Antibody

Gene Names

TP73; P73

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31008 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA31008 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31008 staining K562 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Bcr (Tyr360) Antibody expression in COS7 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Bcr phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Bcr, Polyclonal Antibody (Cat# AAA31008)

Full Name

Phospho-Bcr (Tyr360) Antibody

Gene Names

BCR; ALL; CML; PHL; BCR1; D22S11; D22S662

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31220 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31220 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31220 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31220 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degreeC overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31220 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31220 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Myeloma cells, using OLFM4 Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

OLFM4, Polyclonal Antibody (Cat# AAA31220)

Full Name

OLFM4 Antibody

Gene Names

OLFM4; GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1

Reactivity

Human, Mouse, Rat

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: ftnA antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 24.8 kDaObserved band size: 30 kDa)

Compare (5 max)

ftnA, Polyclonal Antibody (Cat# AAA27053)

Full Name

Rabbit anti-Helicobacter pylori (strain 700392/26695)(Campylobacter pylori) ftnA Polyclonal antibody

Reactivity

Helicobacter pylori

Applications

Western Blot

Purity

Protein A/G

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (TSPAN1 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of TSPAN1 Antibody (Center) in mouse heart tissue lysates (35ug/lane). TSPAN1 (arrow) was detected using the purified Pab.)

Compare (5 max)

TSPAN1, Polyclonal Antibody (Cat# AAA28745)

Full Name

TSPAN1 Antibody (Center)

Gene Names

TSPAN1; NET1; TM4C; TM4SF

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue using at dilution 1/20.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)

Compare (5 max)

ADAMTS13, Polyclonal Antibody (Cat# AAA29726)

Full Name

ADAMTS13 Antibody

Gene Names

ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549 Lane 4: Human kidney.)

WB (Western Blot) (Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:Lane 1: RajiLane 2: MCF-2Lane 3: A549Lane 4: Human kidney)

Compare (5 max)

TGF-Beta 1, Polyclonal Antibody (Cat# AAA29953)

Full Name

TGF-Beta 1 Antibody

Gene Names

TGFB1; CED; LAP; DPD1; TGFB; TGFbeta

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Peptide affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Stomach Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CIRBP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

WB (Western Blot) (Western Blot: Sample: Recombinant CIRBP, Human.)

Compare (5 max)

Cold Inducible RNA Binding Protein, Polyclonal Antibody (Cat# AAA20852)

Full Name

Polyclonal Antibody to Cold Inducible RNA Binding Protein (CIRBP)

Gene Names

CIRBP; CIRP

Reactivity

Human, Mouse, Pig

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Anti-TSLP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-TSLP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-TSLP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Compare (5 max)

TSLP, Polyclonal Antibody (Cat# AAA12321)

Full Name

Rabbit Polyclonal to Human TSLP

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Immunoaffinity Purified

Pricing

$795/0.05 mg $1,520/2x0.05 mg $2,210/3x0.05 mg $2,860/4x0.05 mg $3,260/5x0.05 mg

< >

<< Previous Next >>

Application Data

ELISA

Quality Control Testing

Compare (5 max)

anti-Guinea Pig IgA, Polyclonal Antibody (Cat# AAA14451)

Full Name

HRP Conjugated Sheep anti–Guinea Pig IgA

Applications

Western Blot, Immunohistochemistry

Pricing

$360/0.1 mg $670/2x0.1 mg $965/3x0.1 mg $1,245/4x0.1 mg $1,335/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) ((0.3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot) ((0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Compare (5 max)

GPX4, Polyclonal Antibody (Cat# AAA13650)

Full Name

Goat anti-GPX4 (Isoform a and c) Antibody

Gene Names

GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx

Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Applications

Peptide ELISA, Western Blot, Immunohistochemistry

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$485/0.1 mg $925/2x0.1 mg $1,340/3x0.1 mg $1,725/4x0.1 mg $1,905/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31190 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31190 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31190 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31190 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31190 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31190 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31190 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31190 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from B16F10 cells, using KDM4B / JMJD2B Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

KDM4B/JMJD2B, Polyclonal Antibody (Cat# AAA31190)

Full Name

KDM4B/JMJD2B Antibody

Gene Names

KDM4B; JMJD2B; TDRD14B

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(88%)

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (All lanes: Anti-Zebrafish wnt8a Antibody (Center) at 1:1000-1:2000 dilutionLane 1: zebrafish muscle lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

wnt8a, Polyclonal Antibody (Cat# AAA28796)

Full Name

Zebrafish wnt8a Antibody (Center)

Gene Names

wnt8a; wnt8; wnt-8; wnt8.1; wu:fa20e02; wu:fe05d07

Reactivity

Zebrafish

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Prostate TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SDH AntibodyControl: Used PBS instead of primary antibody Second Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Liver Tissue.)

WB (Western Blot) (Western Blot;Sample:Lane 1: Rat Kidney lysate;Lane 2: Porcine Liver lysate;Lane 3: Porcine Kidney lysate;Lane 4: HepG2 cell lysatePrimary Ab: 0.2ug/ml Rabbit Anti-Human SDH AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

WB (Western Blot) (Western Blot:Sample: Mouse Liver Tissue.)

Compare (5 max)

Sorbitol Dehydrogenase, Polyclonal Antibody (Cat# AAA20921)

Full Name

Polyclonal Antibody to Sorbitol Dehydrogenase (SDH)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

IHC (Immunohistochemistry) (Anti-NOTCH3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Compare (5 max)

NOTCH3, Polyclonal Antibody (Cat# AAA12317)

Full Name

Goat Polyclonal to Human NOTCH3

Gene Names

NOTCH3; IMF2; CASIL; CADASIL

Reactivity

Bat, Human, Monkey, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$720/0.05 mg $1,385/2x0.05 mg $2,005/3x0.05 mg $2,595/4x0.05 mg $2,945/5x0.05 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent analysis of FAP alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of FAP alpha staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of FAP alpha expression in PC3 (A), H9C2 (B), CT26 (C), Hela (D) whole cell lysates.(Predicted band size:87 kD, Observed band size:88kD).)

Compare (5 max)

FAP alpha, Polyclonal Antibody (Cat# AAA27804)

Full Name

Anti-FAP alpha Antibody

Gene Names

FAP; FAPA; SIMP; DPPIV

Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

< >

<< Previous Next >>

Application Data (Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (AAA28682) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.)

Application Data (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Application Data (Fluorescent image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine (50 ?M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28682 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Application Data (AAA28682 staining SQSTM1 in Human prostate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Application Data (All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: PANC-1 whole cell lysate Lane 6: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

SQSTM1 (p62), Polyclonal Antibody (Cat# AAA28682)

Full Name

SQSTM1 (p62) Antibody (C-term)

Gene Names

SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3

Reactivity

Human,Mouse,Rat

Predicted: Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry-Paraffin) (AAA31244 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31244 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31244 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Human brain tumour, using PRDM2 Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

PRDM2, Polyclonal Antibody (Cat# AAA31244)

Full Name

PRDM2 Antibody

Gene Names

PRDM2; RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western Blot;Sample: Recombinant CTLA4, Mouse.)

Compare (5 max)

Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), Polyclonal Antibody (Cat# AAA20303)

Full Name

Polyclonal Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)

Gene Names

Ctla4; Cd152; Ly-56; Ctla-4

Reactivity

Mouse

Applications

Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using CD9 Antibody.)

IHC (Immunohistochemistry) (AAA31074 1/100 staining Human normal tissues adjacent to mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti- Rabbit Ab was used as the secondary Ab.)

IHC (Immunohistochemistry) (AAA31074 at 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of CD9 expression in COLO cells, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31074 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Compare (5 max)

CD9, Polyclonal Antibody (Cat# AAA31074)

Full Name

CD9 Antibody

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31180 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31180 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31180 at 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from K562, using LAP2 alpha Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

LAP2 alpha, Polyclonal Antibody (Cat# AAA31180)

Full Name

LAP2 alpha Antibody

Gene Names

TMPO; TP; LAP2; CMD1T; LEMD4; PRO0868

Reactivity

Human, Mouse, Rat

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31194 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31194 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31194 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31194 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31194 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31194 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Mouse Cerebellum, using VGLUT2 Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from Rat lung, using VGLUT2 Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

VGLUT2, Polyclonal Antibody (Cat# AAA31194)

Full Name

VGLUT2 Antibody

Gene Names

SLC17A6; DNPI; VGLUT2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ACE AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistchemistry))

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Tissue)

WB (Western Blot) (Western Blot: Sample: Recombinant ACE, Human.)

Compare (5 max)

Angiotensin I Converting Enzyme, Polyclonal Antibody (Cat# AAA20954)

Full Name

Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)

Gene Names

ACE; DCP; ACE1; DCP1; CD143

Reactivity

Human

Applications

WB, ICC, IHC, EIA

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$225/0.1 mL $265/0.2 mL $395/0.5 mL $465/1 mL $1,205/5 mL $2,325/2x5 mL $3,385/3x5 mL $4,385/4x5 mL $5,345/5x5 mL

WB (Western Blot) (Figure-1: Western blot analysis of MYADM. Anti-MYADM antibody (AAA14880) was used at 1 ug/ml on h Lung lysate.)

Compare (5 max)

MYADM, Polyclonal Antibody (Cat# AAA14880)

Full Name

Polyclonal Antibody to MYADM

Gene Names

MYADM; SB135

Reactivity

Human

Applications

Western Blot

Purity

Purified
Protein A Chromatography

Pricing

$230/0.025 mg $400/0.1 mg $750/2x0.1 mg $1,090/3x0.1 mg $1,400/4x0.1 mg $1,515/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse aLA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot:Sample: Human Milk; Primary Ab: 2ug/ml Rabbit Anti-Mouse aLA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot:Sample: Recombinant aLA, Mouse.)

WB (Western Blot) (Western Blot:Sample: Bovine Milk;Primary Ab: 2ug/ml Rabbit Anti-Mouse aLA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Compare (5 max)

Alpha-Lactalbumin (aLA), Polyclonal Antibody (Cat# AAA20311)

Full Name

Polyclonal Antibody to Alpha-Lactalbumin (aLA)

Gene Names

Lalba; Lyzg; AW208827

Reactivity

Mouse, Human, Bovine

Applications

Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

< >

<< Previous Next >>

WB (Western Blot) (Gel: 12% SDS-PAGE Lysate: 40 μg Lane 1-2: Hepg2 and 293T cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Esophagus cancer tissue using #AAA29725 at dilution 1/200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Liver tissue using #AAA29725 at dilution 1/200.)

Compare (5 max)

TREM1, Polyclonal Antibody (Cat# AAA29725)

Full Name

TREM1 Antibody

Gene Names

TREM1; CD354; TREM-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(DF13215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31165 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31165 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31165 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Clathrin.Lane 1: 3T3, treated with blocking peptide;Lane 2: 3T3;Lane 3: Pc12.)

Compare (5 max)

Clathrin, Polyclonal Antibody (Cat# AAA31165)

Full Name

Clathrin Antibody

Gene Names

CLTC; Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purifcation

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

IHC (Immunohistchemistry) (AAA28781 staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (All lanes : Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution Lane 1: MCF-7 whole cell lysateLane 2: A431 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

WB (Western Blot) (Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35ug/lane). Cyclin D1(arrow) was detected using the purified Pab.)

Compare (5 max)

Cyclin D1, Polyclonal Antibody (Cat# AAA28781)

Full Name

Cyclin D1 Antibody (S90)

Gene Names

CCND1; BCL1; PRAD1; U21B31; D11S287E

Reactivity

Human, mouse (Predicted Reactivity: Bovine)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of lysates from mouse brain tissue, mouse NIH/3T3, U-251 MG,mouse C2C12 cell line (from left to right), using ENOA Antibody (N-term) (Catalog Number: AAA28805). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term) (Catalog Number: AAA28805). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.)

Compare (5 max)

ENOA, Polyclonal Antibody (Cat# AAA28805)

Full Name

ENOA Antibody (N-term)

Gene Names

ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17

Reactivity

Human, Mouse, Rat
Predicted: Bovine, Monkey

Applications

Immunofluorescence, Western Blot, Flow Cytometry

Purity

Purified Rabbit Polyclnal Antibody (Pab)

Pricing

$505/0.1 mL $965/2x0.1 mL $1,400/3x0.1 mL $1,805/4x0.1 mL $2,025/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (AF488 staining on IF;Sample: Hepa1-6 cellPrimary Ab: 20ug/ml Rabbit Anti-Mouse APOH AntibodySecond Ab: 2ug/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

WB (Western Blot) (Western Blot;Sample: Mouse Kidney lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse APOH AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Mouse Liver TissuePrimary Ab: 20ug/ml Rabbit Anti-Mouse APOH AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

Compare (5 max)

Apolipoprotein H (APOH), Polyclonal Antibody (Cat# AAA20182)

Full Name

Polyclonal Antibody to Apolipoprotein H (APOH)

Gene Names

Apoh; B2GPI; beta2-GPI; beta-2-GPI

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $295/0.2 mL $440/0.5 mL $530/1 mL $1,385/5 mL $2,670/2x5 mL $3,885/3x5 mL $5,035/4x5 mL $6,105/5x5 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry-Paraffin) (AAA31267 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31267 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31267 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31267 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31267 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31267 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from RT4, using PRRG4 Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

PRRG4, Polyclonal Antibody (Cat# AAA31267)

Full Name

PRRG4 Antibody

Gene Names

PRRG4; TMG4; PRGP4

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30960 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30960 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30960 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-Chk2 (Thr68) expression in various lysates)

IF (Immunofluorescence) (AAA30960 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Chk2 phosphorylation expression in UV treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Chk2, Polyclonal Antibody (Cat# AAA30960)

Full Name

Phospho-Chk2 (Thr68) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung tissue using AAA26957 at dilution of 1:100)

WB (Western Blot) (Western blotAll lanes: TMEM64 antibody IgG at 8ug/ml+ Mouse brain tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40,13,15,34 kDaObserved band size: 40 kDa)

Compare (5 max)

TMEM64, Polyclonal Antibody (Cat# AAA26957)

Full Name

TMEM64 Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Mouse Small intestine Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse GPC3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Liver Tissue)

WB (Western Blot) (Western Blot:Sample: Recombinant GPC3, Mouse.)

WB (Western Blot) (Western Blot:Sample: Mouse Liver Tissue.)

Compare (5 max)

Glypican 3, Polyclonal Antibody (Cat# AAA20901)

Full Name

Polyclonal Antibody to Glypican 3 (GPC3)

Gene Names

Gpc3; OCI-5

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $295/0.2 mL $440/0.5 mL $530/1 mL $1,385/5 mL $2,670/2x5 mL $3,885/3x5 mL $5,035/4x5 mL $6,105/5x5 mL

WB (Western Blot) (Western blot analysis on V5-tagged recombinant protein (50kDa) using anti-V5 polyclonal antibody.)

Compare (5 max)

V5-Tag, Polyclonal Antibody (Cat# AAA17983)

Full Name

V5-Tag Antibody

Applications

Western Blot

Purity

Immunogen affinity purified

Pricing

$420/0.1 mL $740/0.1 mL (AF350) $740/0.1 mL (AF405) $740/0.1 mL (AF488) $740/0.1 mL (AF555) $740/0.1 mL (AF568) $740/0.1 mL (Biotin) $740/0.1 mL (FITC) $1,425/2x0.1 mL (AF350) $1,655/5x0.1 mL $2,065/3x0.1 mL (AF350) $2,670/4x0.1 mL (AF350)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse MMP10 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))

WB (Western Blot) (Western Blot:Sample: Mouse Testis lysate;Primary Ab: 2ug/ml Rabbit Anti-Mouse MMP10 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

WB (Western Blot) (Western Blot:Sample: Recombinant MMP10, Mouse.)

Compare (5 max)

Matrix Metalloproteinase 10, Polyclonal Antibody (Cat# AAA20911)

Full Name

Polyclonal Antibody to Matrix Metalloproteinase 10 (MMP10)

Gene Names

Mmp10; SL-2; MMP-10; AV377895

Reactivity

Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$235/0.1 mL $295/0.2 mL $440/0.5 mL $530/1 mL $1,385/5 mL $2,670/2x5 mL $3,885/3x5 mL $5,035/4x5 mL $6,105/5x5 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of CD122 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of CD122 expression in HEK293T (A), Hela (B), rat liver (C) whole cell lysates.)

Compare (5 max)

CD122, Polyclonal Antibody (Cat# AAA27806)

Full Name

Anti-CD122 Antibody

Gene Names

IL2RB; CD122; IL15RB; P70-75

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cell using ATF6 antibody. Blue: DAPI for nuclear staining)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen using ATF6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung using ATF6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer using ATF6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung using ATF6 antibody at dilution of 1:200 (200x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATF6 antibody.)

Compare (5 max)

ATF6, Polyclonal Antibody (Cat# AAA29696)

Full Name

ATF6 Antibody

Gene Names

ATF6; ATF6A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31200 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31200 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31200 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31200 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31200 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31200 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31200 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from A431 cells, using KGF Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

KGF, Polyclonal Antibody (Cat# AAA31200)

Full Name

KGF Antibody

Gene Names

FGF7; KGF; HBGF-7

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31203 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31203 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31203 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using RBM3 Antibody.Lane 1: EC304 cells, treated with blocking peptide;Lane 2: EC304 cells;Lane 3: K562.)

Compare (5 max)

RBM3, Polyclonal Antibody (Cat# AAA31203)

Full Name

RBM3 Antibody

Gene Names

RBM3; RNPL; IS1-RNPL

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

Application Data

Reactivity Chart

Compare (5 max)

UIS4, Polyclonal Antibody (Cat# AAA13862)

Full Name

UIS4 Polyclonal Antibody

Reactivity

Plamodium berghei

Applications

Immunofluorescence

Pricing

$420/0.4 mg $635/1 mg $1,210/2x1 mg $1,750/3x1 mg $2,265/4x1 mg $2,600/5x1 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunofluorescence staining of MCF-7 cells with AAA27006 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of AAA27006 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysateAll lanes: NECTIN4 antibody at 5.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 56, 25 KDaObserved band size: 35 KDa)

Compare (5 max)

NECTIN4, Polyclonal Antibody (Cat# AAA27006)

Full Name

NECTIN4 Antibody

Gene Names

NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

FCM (Flow Cytometry) (Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

WB (Western Blot) (Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) AAA28685. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (CD19 Antibody (N-term) AAA28685 western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).)

WB (Western Blot) (Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). AAA28685 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Compare (5 max)

CD19, Polyclonal Antibody (Cat# AAA28685)

Full Name

CD19 Antibody (N-term)

Gene Names

CD19; B4; CVID3

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31242 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31242 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31242 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31242 at 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31242 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31242 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31242 at 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from C6 cells, using Arc Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

Arc, Polyclonal Antibody (Cat# AAA31242)

Full Name

Arc Antibody

Gene Names

ARC; hArc; Arg3.1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Chicken(88%), Xenopus(83%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing mCherry in neurons (DyGlutmCherry) using 1st Ab anti-mCherry at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®488 at 1/500;)

Application Data (Immunoflourescence in Drosophila larvae NMJ muscle 6/7 expressing mCherry in neurons (DyGlutmCherry) using 1st Ab anti-mCherry at 1/1000 and 2nd Ab anti-goat IgY conjugated to DyLigth488 at 1/500)

Reactivity Chart (+++ "excellent", ++ "good", + "poor", ND not determined)

Compare (5 max)

mCherry, Polyclonal Antibody (Cat# AAA13871)

Full Name

mCherry Polyclonal Antibody

Reactivity

Reacts with Transfected cells proteins

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry

Pricing

$155/0.02 mg (LIMIT 1) $390/0.6 mg $510/1.5 mg $975/2x1.5 mg $1,415/3x1.5 mg $1,825/4x1.5 mg $2,070/5x1.5 mg

WB (Western Blot) (WB: The recombinant protein E7-GST was separated in 10% SDS-PAGE, transferred onto Nitrocellulose membrane, and immunoblotted by Rabbit anti-HPV16/E7 (IN) (Cat#AAA14076) at 1:500. An immune-reactive band is observed at molecular weight ~50 kDa corresponding to the fusion protein size.)

Compare (5 max)

HPV, Polyclonal Antibody (Cat# AAA14076)

Full Name

Rabbit anti HPV (IN) (Human Papillomavirus), E7 protein Polyclonal Antibody

Reactivity

HPV

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Epitope Affinity Purification

Pricing

$390/0.1 mg $730/2x0.1 mg $1,055/3x0.1 mg $1,365/4x0.1 mg $1,445/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31033 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistchemistry) (AAA31033 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31033 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31033 at 1/200 staining Mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31033 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of CDC25B phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-CDC25B (Ser323) expression in various lysates)

Compare (5 max)

CDC25B, Polyclonal Antibody (Cat# AAA31033)

Full Name

Phospho-CDC25B (Ser323) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.