Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testis tissue using AAA26950 at dilution of 1:100)

WB (Western Blot) (Western Blot (WB)All lanes: FLVCR2 antibody at 0.83ug/mlLane 1: A549 whole cell lysateLane 2: Mouse lung tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 58, 36 kDaObserved band size: 58 kDa)

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FLVCR2, Polyclonal Antibody (Cat# AAA26950)

Full Name

FLVCR2 Antibody

Gene Names

FLVCR2; CCT; EPV; PVHH; MFSD7C; C14orf58; FLVCRL14q

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

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IHC (Immunohistochemistry) (ERV3 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERV3 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (ERV3 Antibody (C-Term) western blot analysis in 293,HepG2 cell line lysates (35ug/lane).This demonstrates the ERV3 antibody detected the ERV3 protein (arrow).)

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ERV3, Polyclonal Antibody (Cat# AAA28716)

Full Name

ERV3 Antibody (C-Term)

Gene Names

ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-EGFR (Ser695) expression in various lysates)

IF (Immunofluorescence) (AAA30962 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA30962 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30962 at 1/100 staining human pituitary adenoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30962 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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EGFR, Polyclonal Antibody (Cat# AAA30962)

Full Name

Phospho-EGFR (Ser695) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry-Paraffin) (AAA31276 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31276 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31276 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin) (AAA31276 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31276 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31276 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31276 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using SCAMP2 Antibody.Lane 1: COS, treated with blocking peptide;Lane 2: COS;Lane 3: Mouse testis.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using SCAMP2 Antibody. The lane on the left was treated with blocking peptide.)

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SCAMP2, Polyclonal Antibody (Cat# AAA31276)

Full Name

SCAMP2 Antibody

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry-Paraffin) (Immunohistochemical analysis of paraffin-embedded H. liver section using TYK2 Antibody (C-term) . AAA28807 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

WB (Western Blot) (Western blot analysis of lysates from Hela cell line,mouse liver tissue lysate(from left to right), using TYK2 Antibody (C-term). AAA28807 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

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TYK2, Polyclonal Antibody (Cat# AAA28807)

Full Name

TYK2 Antibody (C-term)

Gene Names

TYK2; JTK1; IMD35

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$505/0.1 mL $965/2x0.1 mL $1,400/3x0.1 mL $1,805/4x0.1 mL $2,025/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)

WB (Western Blot) (Western Blot Validation in Human and Rat Tissue Lysates Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)

WB (Western Blot) (Western Blot Validation in Human Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Application Data (Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and betaactin 3779 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

IF (Immunofluorescence) (Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)

IHC (Immunohistochemistry) (Immunohistochemistry Validation of cIAP in Human Lung Tissue Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/mL, respectively.)

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cIAP, Polyclonal Antibody (Cat# AAA10954)

Full Name

cIAP Antibody

Gene Names

BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

cIAP Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human small intestine using AAA26955 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AAA26955 at dilution 1:100)

WB (Western Blot) (Western blotAll lanes:  PPM1Dantibody at 2.66ug/mlLane 1 : Rat gonadal tissueLane 2 : U87 whole cell lysateLane 3 : 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 67,48 kDaObserved band size: 67 kDa)

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PPM1D, Polyclonal Antibody (Cat# AAA26955)

Full Name

PPM1D Antibody

Gene Names

PPM1D; WIP1; PP2C-DELTA

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

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WB (Western Blot) (SLCO1B3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLCO1B3 antibody detected the SLCO1B3 protein (arrow).)

WB (Western Blot) (All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SW480 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 77 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SLCO1B3 Antibody (C-term) at1:2000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: Hela whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 77 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

WB (Western Blot) (Anti-SLCO1B3 Antibody (C-term)at 1:1000 dilution + NCI-H460 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: PC-3 whole cell lysatesLane 3: SW480 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilutionLane 1: human liver lysatesLane 2: human ovary lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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SLCO1B3, Polyclonal Antibody (Cat# AAA28785)

Full Name

SLCO1B3 Antibody (C-term)

Gene Names

SLCO1B3; LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13

Reactivity

Human

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in various lysates)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining mouse pancreatic tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/100 staining rat Intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in Mouse heart tissue lysate)

IHC (Immunohistchemistry) (AAA31027 at 1/200 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human osteosarcoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human frozen liver tissue section by IHC-Fr. The sample was incubated with the primary antibody (1/200 in BSA) for 1 hour. An Alexa Fluor 488-conjugated Goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31027 at 1/200 staining human myosarcoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human seminoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human vascular cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human placenta tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31027 at 1/200 staining human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PI3K p85 alpha, Polyclonal Antibody (Cat# AAA31027)

Full Name

Phospho-PI3K p85 alpha (Tyr607) Antibody

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Adrenergic Receptor beta2 phosphorylation expression in nocodazole treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30989 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA30989 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA30989 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IF (Immunofluorescence) (AAA30989 staining A549 cells(H2O2 treatment) by IF/ICC. The samples werefixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10%serum for 45 minutes at 25°C. Samples were then incubated with primaryAb(AAA30989) and mouse anti-beta tubulin Ab for 1 hour at 37°C.An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as thesecondary Ab.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (AAA30989 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 26 degree C)

WB (Western Blot) (Western blot analysis of Adrenergic Receptor beta2 phosphorylation expression in nocodazole treated HepG2 whole cell lysates)

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Adrenergic Receptor beta2, Polyclonal Antibody (Cat# AAA30989)

Full Name

Phospho-Adrenergic Receptor beta2 (Ser346) Antibody

Gene Names

ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (AAA31196 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31196) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry-Paraffin) (AAA31196 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31196 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31196 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31196 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31196 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from K562, using PRDM16 Antibody. The lane on the left was treated with blocking peptide.)

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PRDM16, Polyclonal Antibody (Cat# AAA31196)

Full Name

PRDM16 Antibody

Gene Names

PRDM16; MEL1; KMT8F; LVNC8; PFM13; CMD1LL

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Positive WB detected in Recombinant proteinAll lanes: gB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43.3 kDaObserved band size: 46 kDa)

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gB, Polyclonal Antibody (Cat# AAA27058)

Full Name

Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) gB Polyclonal Antibody

Reactivity

Epstein-Barr virus

Applications

Western Blot

Purity

Protein G

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

IF (Immunofluorescence) (Immunofluorescent analysis of HepG-2 cells using AAA26914 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

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ZNRF3, Polyclonal Antibody (Cat# AAA26914)

Full Name

ZNRF3 Antibody

Gene Names

ZNRF3; RNF203; BK747E2.3

Reactivity

Human

Applications

Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western blot analysis of RPL22/L1 using K562 whole cell lysates)

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RPL22/L1, Polyclonal Antibody (Cat# AAA31142)

Full Name

RPL22/L1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (AAA31206 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31206) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31206 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using Activin Receptor Type IA Antibody. The lane on the left was treated with blocking peptide.)

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Activin Receptor Type IA, Polyclonal Antibody (Cat# AAA31206)

Full Name

Activin Receptor Type IA Antibody

Gene Names

ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry-Paraffin) (AAA31208 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31208 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31208 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31208 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31208 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Rat brain, using SV2A Antibody. The lane on the left was treated with blocking peptide.)

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SV2A, Polyclonal Antibody (Cat# AAA31208)

Full Name

SV2A Antibody

Gene Names

SV2A; SV2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)

Applications

ELISA

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western blot analysis of various lysates, using TIPARP Rabbit pAb (AAA28273) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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TIPARP, Polyclonal Antibody (Cat# AAA28273)

Full Name

TIPARP Polyclonal Antibody

Gene Names

TIPARP; PARP7; ARTD14; pART14

Applications

Western Blot

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

WB (Western Blot) (Western blot analysis of AmCyan recombinant protein, using AAA29659 diluted at 1:5,000.)

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AmCyan, Polyclonal Antibody (Cat# AAA29659)

Full Name

AmCyan Rabbit Polyclonal Antibody

Applications

Western Blot

Purity

Affinity purification using immunogen.

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IHC (Immunohistochemistry) (AAA30985 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Keratin 8 phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30985 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA30985 staining Hela cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA30985 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30985 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Keratin 8 phosphorylation expression in EGF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Keratin 8, Polyclonal Antibody (Cat# AAA30985)

Full Name

Phospho-Keratin 8 (Ser432) Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry) (AAA31083 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 92)

IHC (Immunohistchemistry) (AAA31083 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 91)

IF (Immunofluorescence) (AAA31083 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA31083 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of EGFR expression in A431 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31083 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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EGFR, Polyclonal Antibody (Cat# AAA31083)

Full Name

EGFR Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western blot analysis of lung1 lysate using FGF10 antibody. The lane on the left is treated with the antigen-specific peptide.)

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FGF10, Polyclonal Antibody (Cat# AAA31123)

Full Name

FGF10 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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FCM (Flow Cytometry) (BDKRB1 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (All lanes : Anti-BDK_1 Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

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BDK_1, Polyclonal Antibody (Cat# AAA28760)

Full Name

BDK_1 Antibody (Center)

Gene Names

BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Western Blot analysis of SARS-CoV-2 Nucleocapsid Protein: Anti- SARS-CoV-2 Nucleocapsid Protein (AAA14886) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.)

WB (Western Blot) (Western Blot analysis of Nucleocapsid antibody: Anti- Nucleocapsid antibody (SARS-CoV-2) was used at 4 ug/ml on recombinant Nucleocapsid Protein.)

Application Data (Wells of a 96-microtiter plate were coated with 4 ug/ml of SARS-Cov-2/nCov/COVID-19 Nucleocapsid recombinant protein . The binding was detected by addition of different dilution of AAA14886 polyclonal antibody. The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.)

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COVID 19 Nucleocapsid (NP) Coronavirus, Polyclonal Antibody (Cat# AAA14886)

Full Name

Coronavirus (COVID-19) Nucleocapsid Antibody

Applications

Western Blot

Purity

Protein A Chromatography

Pricing

$230/0.025 mg $400/0.1 mg $750/2x0.1 mg $1,090/3x0.1 mg $1,400/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunofluorescence) (Figure 15. IF analysis of ARHGEF7 using anti-ARHGEF7 antibody (AAA19780).ARHGEF7 was detected in a paraffin-embedded section of human prostste cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 14. IF analysis of ARHGEF7 using anti-ARHGEF7 antibody (AAA19780).ARHGEF7 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 13. Flow Cytometry analysis of THP-1 cells using anti-ARHGEF7 antibody (AAA19780).Overlay histogram showing THP-1 cells stained with AAA19780 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF7 Antibody (AAA19780, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 12. IF analysis of ARHGEF7 using anti-ARHGEF7 antibody (AAA19780).ARHGEF7 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of ARHGEF7 using anti-ARHGEF7 antibody (AAA19780).ARHGEF7 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse skeletal muscle lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF7 antigen affinity purified polyclonal antibody (#AAA19780) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARHGEF7 at approximately 80 kDa. The expected band size for ARHGEF7 is at 90 kDa.)

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ARHGEF7, Polyclonal Antibody (Cat# AAA19780)

Full Name

Anti-ARHGEF7 Antibody Picoband

Gene Names

ARHGEF7; P50; P85; PAK3; PIXB; COOL1; P50BP; COOL-1; P85SPR; BETA-PIX; P85COOL1; Nbla10314

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (MCOLN1 Antibody (C-term) (AAA28690)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCOLN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (MCOLN1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MCOLN1 antibody detected the MCOLN1 protein (arrow).)

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MCOLN1, Polyclonal Antibody (Cat# AAA28690)

Full Name

MCOLN1 Antibody (C-term)

Gene Names

MCOLN1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1

Reactivity

Human
Predicted Reactivity: Monkey, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IF (Immunofluorescence) (AAA31071 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (HeLa cells treated with EGF)

IHC (Immunohistochemistry) (AAA31071 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of HER4 phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA31071 at 1/100 staining human breast carcinoma tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 ho)

WB (Western Blot) (Western blot analysis of Phospho-HER4 (Tyr1284) expression in various lysates)

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HER4, Polyclonal Antibody (Cat# AAA31071)

Full Name

Phospho-HER4 (Tyr1284) Antibody

Gene Names

ERBB4; HER4; ALS19; p180erbB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of Taurine Transporter staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Taurine Transporter expression in Jurkat (A) whole cell lysates.)

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Taurine Transporter, Polyclonal Antibody (Cat# AAA27812)

Full Name

Anti-Taurine Transporter Antibody

Gene Names

SLC6A6; TAUT

Reactivity

Human, Mouse, Rat, Dog

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was purified by affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

WB (Western Blot) (Western blot analysis of lysate from A431 cell line, using SPRR2A Antibody (C-term). AAA28741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

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SPRR2A, Polyclonal Antibody (Cat# AAA28741)

Full Name

SPRR2A Antibody (C-term)

Reactivity

Human

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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FCM (Flow Cytometry) (WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

IHC (Immunohistochemistry) (WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Immunohistochemical analysis of AAA28757 on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

WB (Western Blot) (All lanes : Anti-MCSF Receptor (CSF1R) Antibody (C-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: rat spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

FCM (Flow Cytometry) (MCSF Receptor (CSF1R) Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of CSF1R (arrow) using rabbit polyclonal CSF1R Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSF1R gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

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MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)

Full Name

MCSF Receptor (CSF1R) Antibody (C-term)

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-HRD1 Pab is used in Western blot to detect HRD1 in mouse kidney tissue lysate.)

WB (Western Blot) (Western blot analysis of SYVN1 (HRD1) Antibody (C-term) in T47D cell line lysates (35ug/lane). HRD1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (# AAA28764, 1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28764 SYVN1 (HRD1) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)

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SYVN1 (HRD1), Polyclonal Antibody (Cat# AAA28764)

Full Name

SYVN1 (HRD1) Antibody (C-term)

Gene Names

SYVN1; DER3; HRD1

Reactivity

Human, mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-ROBO1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

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ROBO1, Polyclonal Antibody (Cat# AAA12322)

Full Name

Goat Polyclonal to Human ROBO1

Gene Names

ROBO1; SAX3; DUTT1

Reactivity

Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$720/0.05 mg $1,385/2x0.05 mg $2,005/3x0.05 mg $2,595/4x0.05 mg $2,945/5x0.05 mg

WB (Western Blot) (Western Blot:C: 293 cell extract controlE: 293 cell expressing p27 (SIV/mac239))

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p27 (SIV/mac239), Polyclonal Antibody (Cat# AAA13724)

Full Name

Anti-p27 (SIV/mac239), rabbit IgG

Applications

Western Blot

Purity

Immunoaffinity chromatography.

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,575/5x0.1 mg

WB (Western Blot) (Western Blot:1: 293 cell extract control2: 293 cell expressing Pol (SIV/mac239))

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Pol (SIV/mac239), Polyclonal Antibody (Cat# AAA13725)

Full Name

Anti-Pol (SIV/mac239), rabbit IgG

Reactivity

Cross-reactivity to other subtypes not tested.

Applications

Western Blot

Purity

Immunoaffinity chromatography.

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,575/5x0.1 mg

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WB (Western Blot) (Host: Rabbit Target Name: FOSL1 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-FOSL1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RatTarget Name: FOSL1Sample Tissue: Rat Skeletal MuscleAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-FOSL1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder TissueObserved Staining: NucleusPrimary Antibody Concentration: 1:100Other Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (Rabbit Anti-FOSL1 AntibodyParaffin Embedded Tissue: Human alveolar cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-FOSL1 AntibodyParaffin Embedded Tissue: Human alveolar cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-FOSL1 antibody)

IHC (Immunohistochemistry) (Human Skin)

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FOSL1, Polyclonal Antibody (Cat# AAA23383)

Full Name

FOSL1 antibody - middle region

Gene Names

FOSL1; FRA; FRA1; fra-1

Reactivity

Tested Species Reactivity Human, Rat
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of PRC1 (Phospho-Thr481) using IFN-α treated HuvEc whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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PRC1, Polyclonal Antibody (Cat# AAA31450)

Full Name

Phospho-PRC1 (Thr481) Antibody

Gene Names

PRC1; ASE1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Horse (90%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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Application Data (AAA31369 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31369 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31369 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from NIH 3T3, using Phospho-FoxM1 (Ser35) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

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FOXM1, Polyclonal Antibody (Cat# AAA31369)

Full Name

Phospho-FOXM1 (Ser35) Antibody

Gene Names

FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (AAA31216 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31216) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31216 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31216 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31216 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells, using FKBP25 Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, using FKBP25 Antibody. The lane on the left was treated with blocking peptide.)

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FKBP25, Polyclonal Antibody (Cat# AAA31216)

Full Name

FKBP25 Antibody

Gene Names

FKBP3; FKBP-3; FKBP25; PPIase; FKBP-25

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western Blot of KK-LC-1 Antibody (AAA14411) with: 1) THP1 cells2)THP1 cells treated with LPS1:250 antibody dilution in DiluOBufferTM)

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KK-LC-1, Polyclonal Antibody (Cat# AAA14411)

Full Name

KK-LC-1 Antibody

Gene Names

CT83; KKLC1; CXorf61; KK-LC-1

Reactivity

Human, Monkey

Applications

Immunoprecipitation, Western Blot

Pricing

$520/0.1 mg $985/2x0.1 mg $1,425/3x0.1 mg $1,845/4x0.1 mg $2,030/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Anti-CHRM4 / M4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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CHRM4 / M4, Polyclonal Antibody (Cat# AAA12369)

Full Name

Rabbit Polyclonal (IgG) to Human CHRM4 / M4

Gene Names

CHRM4; HM4; M4R

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Peptide ELISA

Purity

Immunoaffinity Purified

Pricing

$690/0.05 mL $1,325/2x0.05 mL $1,915/3x0.05 mL $2,475/4x0.05 mL $2,800/5x0.05 mL

Application Data

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c-Src (Tyr-215), Polyclonal Antibody (Cat# AAA14100)

Full Name

c-Src (Tyr-215), phospho-specific [Conserved site]

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Pricing

$535/0.1 mL $1,015/2x0.1 mL $1,470/3x0.1 mL $1,900/4x0.1 mL $2,125/5x0.1 mL

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IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30986 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30986 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30986 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Phospho-Fyn (Tyr530) expression in various lysates)

IHC (Immunohistochemistry) (AAA30986 at 1/100 staining human liver cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours)

IF (Immunofluorescence) (AAA30986 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Fyn phosphorylation expression in H2O2 treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Fyn, Polyclonal Antibody (Cat# AAA30986)

Full Name

Phospho-Fyn (Tyr530) Antibody

Gene Names

FYN; SLK; SYN; p59-FYN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry) (Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)

IHC (Immunohistochemistry) (Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Mouse Spleen Tissue)

WB (Western Blot) (Anti- S100A9 Picoband antibody, AAA11645, Western blottingAll lanes: Anti S100A9 (AAA11645) at 0.5ug/mlWB: Mouse Ovary Tissue Lysate at 50ugPredicted bind size: 13KDObserved bind size: 13KD)

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S100A9, Polyclonal Antibody (Cat# AAA11645)

Full Name

Anti-S100A9 Antibody

Gene Names

S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with AAA28706 NANOG (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA28706 NANOG (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.)

FCM (Flow Cytometry) (NANOG Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (All lanes : Anti-NANOG Antibody (N-term) at 1:1000 dilutionLane 1: A2780 whole cell lysateLane 2: OVCAR3 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Western blot analysis of anti-NANOG Antibody (N-term) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).)

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NANOG, Polyclonal Antibody (Cat# AAA28706)

Full Name

NANOG Antibody (N-term)

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunhistochemistry)

IHC (Immunhistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

IF (Immunofluorescence)

IF (Immunofluorescence)

IF (Immunofluorescence)

IF (Immunofluorescence)

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PPAR-alpha, Polyclonal Antibody (Cat# AAA29102)

Full Name

PPAR-alpha Polyclonal Antibody

Gene Names

PPARA; PPAR; NR1C1; hPPAR; PPARalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

ICC (Immunocytochemistry) (Immunofluorescence analysis of NIH/3T3 cells using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from THP-1 cells using cGAS Rabbit pAb (AAA28207) at1:1000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of lysates from HT-29 cells using cGAS Rabbit pAb (AAA28207) at 1:1000 dilution incubated overnight at 4°C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (AAA28207) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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MB21D1, Polyclonal Antibody (Cat# AAA28207)

Full Name

MB21D1 Polyclonal Antibody

Gene Names

MB21D1; cGAS; h-cGAS; C6orf150

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (AAA31204 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31204 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry-Paraffin) (AAA31204 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31204 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31204 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin) (AAA31204 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from K562, using MSH4 Antibody. The lane on the left was treated with blocking peptide.)

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MSH4, Polyclonal Antibody (Cat# AAA31204)

Full Name

MSH4 Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of PGD2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

WB (Western Blot) (Western blot analysis of PGD2 Receptor expression in HEK293T (A), Hela (B), HepG2 (C), mouse lung (D), mouse brain (E), mouse kidney (F), rat kidney (G) whole cell lysates.)

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PGD2 Receptor, Polyclonal Antibody (Cat# AAA27807)

Full Name

Anti-PGD2 Receptor Antibody

Gene Names

PTGDR; DP; AS1; DP1; ASRT1; PTGDR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

WB (Western Blot) (All lanes : Anti-TIGIT Antibody at 1:2000 dilutionLane 1: EL4 whole cell lysateLane 2: human fetal thymus lysateLane 3: human spleen lysateLane 4: HUT78 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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TIGIT, Polyclonal Antibody (Cat# AAA28803)

Full Name

TIGIT Antibody

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.