Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemisry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human heart, myocardial infarct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-EDG7 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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LPAR3 / LPA3 / EDG7, Polyclonal Antibody (Cat# AAA52233)

Full Name

Rabbit Polyclonal to Human LPAR3 / LPA3 / EDG7

Gene Names

LPAR3; EDG7; GPCR; LPA3; Edg-7; LP-A3; HOFNH30; RP4-678I3

Reactivity

Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Mouse
Predicted Reactivity: Monkey, Rat, Hamster (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$645/0.05 mg $1,250/2x0.05 mg $1,830/3x0.05 mg $2,375/4x0.05 mg $2,670/5x0.05 mg

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse stomach using GLS antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using GLS antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GLS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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GLS, Polyclonal Antibody (Cat# AAA37549)

Full Name

GLS Polyclonal Antibody

Gene Names

GLS; GAC; GAM; KGA; GLS1; AAD20

Reactivity

Human, Mouse

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA241652(RNF139 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241652(RNF139 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))

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RNF139, Polyclonal Antibody (Cat# AAA241652)

Full Name

RNF139 Antibody

Gene Names

RNF139; RCA1; TRC8; HRCA1

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Ab-1248) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1248) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody.)

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ERBB2, Polyclonal Antibody (Cat# AAA242742)

Full Name

ERBB2 (Ab-1248) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/40.)

Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): Human brain malignant glioma tissue Amount of lysate: 40ug per lane Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

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P2RY6, Polyclonal Antibody (Cat# AAA304250)

Full Name

P2RY6 Antibody

Gene Names

P2RY6; P2Y6

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Tonsil, plasma cells, using beta A4 Amyloid antibody(10 ug/ml).)

Application Data (Sample(30 ug of whole cell lysate)A: Hep G27.5% SDS PAGEPrimary antibody diluted at 1: 1500)

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beta-Amyloid, Polyclonal Antibody (Cat# AAA304845)

Full Name

beta-Amyloid antibody

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by antigen-affinity chromatography.

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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Application Data

Application Data

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ACADM, Polyclonal Antibody (Cat# AAA307638)

Full Name

ACADM Antibody

Gene Names

ACADM; MCAD; ACAD1; MCADH

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified

Pricing

$455/0.1 mL $890/2x0.1 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,870/5x0.1 mL

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IHC (Immunohiostchemistry) (Complement C2 Antibody-Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (Complement C2 Antibody-Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.)

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Complement C2, Polyclonal Antibody (Cat# AAA162875)

Full Name

Complement C2 Rabbit anti-Human Polyclonal Antibody

Gene Names

C2; CO2; ARMD14

Reactivity

Mouse, Rat, Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Affinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of PRKAA2 (arrow) using rabbit polyclonal PRKAA2 Antibody (C-term) (RB11657). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2) (Origene Technologies).)

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AMPK alpha2 (PRKAA2), Polyclonal Antibody (Cat# AAA289494)

Full Name

AMPK alpha2 (PRKAA2) Antibody (C-term)

Gene Names

PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (TLL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TLL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of TLL2 (arrow) using rabbit polyclonal TLL2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TLL2 gene.)

WB (Western Blot) (TLL2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TLL2 antibody detected the TLL2 protein (arrow).)

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TLL2, Polyclonal Antibody (Cat# AAA289657)

Full Name

TLL2 Antibody (N-term)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (RAB3IL1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (RAB3IL1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RAB3IL1 antibody detected the RAB3IL1 protein (arrow).)

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RAB3IL1, Polyclonal Antibody (Cat# AAA288600)

Full Name

RAB3IL1 Antibody (C-term)

Gene Names

RAB3IL1; GRAB

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using Collagen IV Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen IV Antibody. The picture on the right is blocked with the synthesized peptide.)

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Collagen IV, Polyclonal Antibody (Cat# AAA317183)

Full Name

Collagen IV Antibody

Gene Names

COL4A1; BSVD; RATOR

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (AAA320777 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemisry) (AAA320777 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from HepG2, using Claudin 3 Antibody. Lane 1 was treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis on COLO205 cell lysate using Claudin 3 Antibody)

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Claudin 3, Polyclonal Antibody (Cat# AAA320777)

Full Name

Claudin 3 Antibody

Gene Names

CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2

Reactivity

Human, Mouse, Rat
Predicted: Guinea Pig

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of A2780 cells, using PRSS8 antibody.)

IHC (Immunohistochemistry) (AAA323048 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PRSS8, Polyclonal Antibody (Cat# AAA323048)

Full Name

PRSS8 Antibody

Gene Names

PRSS8; CAP1; PROSTASIN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of HSPB9 expression in A549 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA323453 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HSPB9, Polyclonal Antibody (Cat# AAA323453)

Full Name

HSPB9 Antibody

Gene Names

HSPB9; CT51

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using SERPINE2 Polyclonal Antibody at dilution 1:20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using SERPINE2 Polyclonal Antibody at dilution 1:20)

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SERPINE2, Polyclonal Antibody (Cat# AAA167799)

Full Name

SERPINE2 Polyclonal Antibody

Gene Names

SERPINE2; GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

WB (Western Blot) (Western blot analysis of extracts of various cell lines using H2-Aa Polyclonal Antibody at 1:1000 dilution.)

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H2-Aa, Polyclonal Antibody (Cat# AAA179161)

Full Name

H2-Aa Polyclonal Antibody

Gene Names

H2-Aa; Ia1; H2Aa; Ia-1; H-2Aa; Aalpha; IAalpha; I-Aalpha

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Anti-CD26 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CD26 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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DPP4 / CD26, Polyclonal Antibody (Cat# AAA51672)

Full Name

Rabbit Polyclonal to Human DPP4 / CD26

Gene Names

DPP4; CD26; ADABP; ADCP2; DPPIV; TP103

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Chimpanzee, Orangutan, Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.)

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p53, Polyclonal Antibody (Cat# AAA318014)

Full Name

p53 Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of B-RAF expression in Insulin treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA322227 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA322227 at 1/100 staining human liver cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours)

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B-RAF, Polyclonal Antibody (Cat# AAA322227)

Full Name

B-RAF Antibody

Gene Names

BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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LATS1/2, Polyclonal Antibody (Cat# AAA319471)

Full Name

LATS1/2 (Phospho-Ser909) Antibody

Gene Names

LATS1; wts; WARTS

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of A431 cells treated with EGF, using Phospho-Afadin (Ser1799) Antibody.)

IHC (Immunohistochemistry) (AAA321299 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Afadin, Polyclonal Antibody (Cat# AAA321299)

Full Name

Phospho-Afadin (Ser1799) Antibody

Gene Names

AFDN; AF6; MLLT4; MLL-AF6; l-afadin

Reactivity

Human, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells using ABCC3 antibody.)

IF (Immunofluorescence) (AAA324547 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA324547 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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ABCC3, Polyclonal Antibody (Cat# AAA324547)

Full Name

ABCC3 Antibody

Gene Names

ABCC3; MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from K562 cells, using CYP2D6 antibody.)

IHC (Immunohiostchemistry) (AAA325380 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA325380 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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Cytochrome P450 2D6, Polyclonal Antibody (Cat# AAA325380)

Full Name

Cytochrome P450 2D6 Antibody

Gene Names

CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of 293 cell extracts treated with EGF (200ng/ml, 30mins), using WNK1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326771 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326771 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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WNK1, Polyclonal Antibody (Cat# AAA326771)

Full Name

WNK1 Antibody

Gene Names

WNK1; KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA327173 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Cryopyrin using HT-29 whole cell lysates)

IHC (Immunohistochemistry) (AAA327173 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Cryopyrin, Polyclonal Antibody (Cat# AAA327173)

Full Name

Cryopyrin Antibody

Gene Names

NLRP3; AII; AVP; FCU; MWS; FCAS; KEFH; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; DFNA34; PYPAF1; AGTAVPRL

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) (Western blot analysis of Septin 6 expression in Jurkat (A), NIH3T3 (B), PC12 (C) whole cell lysates.)

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Septin 6, Polyclonal Antibody (Cat# AAA105179)

Full Name

Anti-Septin 6 Antibody

Gene Names

SEPT6; SEP2; SEPT2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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Application Data

Application Data

Application Data

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GFP, Polyclonal Antibody (Cat# AAA76851)

Full Name

anti-GFP Antibody (Chicken)-Affinity Purified

Reactivity

GFP

Applications

Western Blot, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$870/0.5 mg $1,690/2x0.5 mg $2,465/3x0.5 mg $3,200/4x0.5 mg $3,695/5x0.5 mg

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IHC (Immunohistochemisry)

IHC (Immunohiostchemistry)

IHC (Immunohistochemistry)

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Gastrin, Polyclonal Antibody (Cat# AAA75884)

Full Name

Gastrin Antibody Affinity Purified

Gene Names

GAST; GAS

Reactivity

Human, Monkey, Rat

Applications

Western Blot, Immunomicroscopy, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Confocal Microscopy

Pricing

$490/0.1 mg $955/2x0.1 mg $1,400/3x0.1 mg $1,815/4x0.1 mg $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240227(MAP3K4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA240227(MAP3K4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

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MAP3K4, Polyclonal Antibody (Cat# AAA240227)

Full Name

MAP3K4 Antibody

Gene Names

MAP3K4; MTK1; MEKK4; MAPKKK4; PRO0412

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 30 ug, Lane: Mouse crassum intestinum tissue, Primary antibody: AAA240106(CKMT1A/CKMT1B Antibody) at dilution 1/1500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA240106(CKMT1A/CKMT1B Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using AAA240106(CKMT1A/CKMT1B Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CKMT1A, Polyclonal Antibody (Cat# AAA240106)

Full Name

CKMT1A Antibody

Gene Names

CKMT1A; CKMT1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-3: K562 cells, hela cells, 293T cells, Primary antibody: AAA240160(PRMT5 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using AAA240160(PRMT5 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA240160(PRMT5 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PRMT5, Polyclonal Antibody (Cat# AAA240160)

Full Name

PRMT5 Antibody

Gene Names

PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 8%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, LO2 cells, Lovo cells,Primary antibody: AAA241838(ISM2 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241838(ISM2 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241838(ISM2 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200))

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ISM2, Polyclonal Antibody (Cat# AAA241838)

Full Name

ISM2 Antibody

Gene Names

ISM2; TAIL1; THSD3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from K562 cells (Lane 2), using Retinoic Acid Receptor beta antiobdy. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using Retinoic Acid Receptor beta antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Retinoic Acid Receptor beta antibody.)

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RARB, Polyclonal Antibody (Cat# AAA242891)

Full Name

RARB Antibody

Gene Names

RARB; HAP; RRB2; NR1B2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TEAD3 Polyclonal Antibody at dilution of 1:50(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using TEAD3 Polyclonal Antibody at dilution of 1:50(×200))

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TEAD3, Polyclonal Antibody (Cat# AAA176551)

Full Name

TEAD3 Polyclonal Antibody

Gene Names

TEAD3; TEF5; TEAD5; TEF-5; DTEF-1; ETFR-1; TEAD-3

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-AQP11 antibody (AAA126597).Overlay histogram showing Caco-2 cells stained with AAA126597 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP11 Antibody (AAA126597, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of AQP11 using anti-AQP11 antibody (AAA126597).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP11 antigen affinity purified polyclonal antibody (#AAA126597) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AQP11 at approximately 53 kDa. The expected band size for AQP11 is at 53 kDa.)

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AQP11, Polyclonal Antibody (Cat# AAA126597)

Full Name

Anti-AQP11 Antibody Picoband

Gene Names

AQP11; AQPX1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using AHNAK Polyclonal Antibody at dilution of 1:50(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AHNAK Polyclonal Antibody at dilution of 1:50(×200))

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AHNAK, Polyclonal Antibody (Cat# AAA175575)

Full Name

AHNAK Polyclonal Antibody

Gene Names

AHNAK; PM227; AHNAKRS

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human placenta using AAA118334 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testis tissue using AAA118334 at dilution 1:100)

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DPPA4, Polyclonal Antibody (Cat# AAA118334)

Full Name

DPPA4 Antibody

Gene Names

DPPA4; 2410091M23Rik

Reactivity

Human

Applications

Immunohistochemistry

Purity

>95%, Protein G Purified

Pricing

$235/0.05 mg $345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,490/5x0.1 mg

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WB (Western Blot) (Western blot analysis of Jurkat whole cell lysates, using PTPRC Antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA326598 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326598 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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PTPRC, Polyclonal Antibody (Cat# AAA326598)

Full Name

PTPRC Antibody

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of CLAUDIN4 in human colon tissue lysate with CLAUDIN4 antibody at 1 ug/ml)

IF (Immunofluorescence) (Immunofluorescence of CLAUDIN4 in human colon tissue with CLAUDIN4 antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-CLDN4 / Claudin 4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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CLDN4 / Claudin 4, Polyclonal Antibody (Cat# AAA52527)

Full Name

Rabbit Polyclonal to Human CLDN4 / Claudin 4

Gene Names

CLDN4; CPER; CPE-R; CPETR; CPETR1; WBSCR8; hCPE-R

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$765/0.05 mg $1,495/2x0.05 mg $2,185/3x0.05 mg $2,835/4x0.05 mg $3,235/5x0.05 mg

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IHC (Immunohistochemistry) (Anti-EDG7 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemisry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human heart, diabetes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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LPAR3 / LPA3 / EDG7, Polyclonal Antibody (Cat# AAA52090)

Full Name

Rabbit Polyclonal to Human LPAR3 / LPA3 / EDG7

Gene Names

LPAR3; EDG7; GPCR; LPA3; Edg-7; LP-A3; HOFNH30; RP4-678I3

Reactivity

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohiostchemistry) (DAB staining on IHC-P; Samples: Human Lung cancer Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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TNF Receptor Associated Factor 5 (TRAF5), Polyclonal Antibody (Cat# AAA132412)

Full Name

Polyclonal Antibody to TNF Receptor Associated Factor 5 (TRAF5)

Gene Names

TRAF5; RNF84; MGC:39780

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0. 05ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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CYP2E1, Polyclonal Antibody (Cat# AAA61528)

Full Name

Goat anti-CYP2E1 Antibody

Gene Names

CYP2E1; CPE1; CYP2E; P450-J; P450C2E

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

WB (Western Blot) ((0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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F11R/JAM-A, Polyclonal Antibody (Cat# AAA61404)

Full Name

Goat anti-F11R/JAM-A Antibody

Gene Names

F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using ZC3H12D Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using ZC3H12D Polyclonal Antibody at 1:1000 dilution.)

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ZC3H12D, Polyclonal Antibody (Cat# AAA179131)

Full Name

ZC3H12D Polyclonal Antibody

Gene Names

ZC3H12D; TFL; p34; MCPIP4; C6orf95; dJ281H8.1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of PC-3 cells using anti-CEA/CEACAM5 antibody (AAA126981).Overlay histogram showing PC-3 cells stained with AAA126981 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CEA/CEACAM5 Antibody (AAA126981, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (AAA126981).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Hacat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (#AAA126981) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.)

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CEA/CEACAM5, Polyclonal Antibody (Cat# AAA126981)

Full Name

Anti-CEA/CEACAM5 Antibody Picoband

Gene Names

CEACAM5; CEA; CD66e

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (WFibody) at dilution 1/40.)

IHC (Immunohistochemistry) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (WFibody) at dilution 1/40.)

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WFDC3, Polyclonal Antibody (Cat# AAA311858)

Full Name

WFDC3 Antibody

Gene Names

WFDC3; WAP14; dJ447F3.3

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Mouse Dapk3 Antibody (Center) (AAA285249)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Dapk3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Mouse Dapk3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)

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Dapk3, Polyclonal Antibody (Cat# AAA285249)

Full Name

Mouse Dapk3 Antibody (Center)

Gene Names

Dapk3; dlk; ZIPK

Reactivity

Mouse (Predicted Reactivity: Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.