Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-PBLD antibody (AAA127633).Overlay histogram showing SiHa cells stained with AAA127633 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PBLD Antibody (AAA127633, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PBLD using anti-PBLD antibody (AAA127633).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.Lane 3: rat liver tissue lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (#AAA127633) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.)

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PBLD, Polyclonal Antibody (Cat# AAA127633)

Full Name

Anti-PBLD Antibody Picoband

Gene Names

PBLD; MAWBP; MAWDBP

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Anti-DRD4 / Dopamine Receptor D4 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemisry) (Anti-DRD4 / Dopamine Receptor D4 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-DRD4 / Dopamine Receptor D4 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-DRD4 / Dopamine Receptor D4 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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DRD4 / Dopamine Receptor D4, Polyclonal Antibody (Cat# AAA51527)

Full Name

Rabbit Polyclonal to Human DRD4 / Dopamine Receptor D4

Gene Names

DRD4; D4DR

Reactivity

Gibbon, Gorilla, Human, Monkey

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$645/0.05 mg $1,250/2x0.05 mg $1,830/3x0.05 mg $2,375/4x0.05 mg $2,670/5x0.05 mg

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WB (Western Blot) (Western blot analysis of PRPF31 using HeLa whole cell lysates)

IF (Immunofluorescence) (AAA322600 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA322600 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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PRPF31, Polyclonal Antibody (Cat# AAA322600)

Full Name

PRPF31 Antibody

Gene Names

PRPF31; RP11; PRP31; SNRNP61; NY-BR-99

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human and mouse UBL7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UBL7 antibody AAA212084 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UBL7 was also immunoprecipitated by rabbit anti-UBL7 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)

IHC (Immunohiostchemistry) (Detection of human UBL7 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-UBL7 (Cat. No. AAA212084 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human UBL7 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-UBL7 (Cat. No. AAA212084 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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UBL7, Polyclonal Antibody (Cat# AAA212084)

Full Name

Rabbit anti-UBL7 Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $605/0.1 mg $940/5x0.01 mg $1,170/2x0.1 mg $1,710/3x0.1 mg $2,220/4x0.1 mg $2,475/5x0.1 mg

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IHC (Immunohiostchemistry) (AAA323513 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of ATR expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)

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ATR, Polyclonal Antibody (Cat# AAA323513)

Full Name

ATR Antibody

Gene Names

ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0. 03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GPR94/TRA1, Polyclonal Antibody (Cat# AAA60916)

Full Name

Goat anti-GPR94/TRA1 Antibody

Gene Names

HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m

Reactivity

Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

WB (Western Blot) (Western blot analysis of extracts of mouse lung, using RGS16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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RGS16, Polyclonal Antibody (Cat# AAA37539)

Full Name

RGS16 Polyclonal Antibody

Gene Names

RGS16; RGS-R; A28-RGS14; A28-RGS14P

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of JPH4 in Human Brain cells with JPH4 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of JPH4 in human brain tissue with JPH4 antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of JPH4 in 293 cell lysate with JPH4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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JPH4, Polyclonal Antibody (Cat# AAA40025)

Full Name

JPH4 Antibody

Gene Names

JPH4; JP4; JPHL1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

JPH4 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of Jurkat cells using anti-SIRT5 antibody (AAA126222).Overlay histogram showing Jurkat cells stained with AAA126222 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody (AAA126222, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HL-60 cells using anti-SIRT5 antibody (AAA126222).Overlay histogram showing HL-60 cells stained with AAA126222 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody (AAA126222, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of SIRT5 using anti-SIRT5 antibody (AAA126222).SIRT5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SIRT5 Antibody (AAA126222) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of SIRT5 using anti-SIRT5 antibody (AAA126222).SIRT5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SIRT5 Antibody (AAA126222) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SIRT5 using anti-SIRT5 antibody (AAA126222).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody (#AAA126222) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT5 at approximately 34 kDa. The expected band size for SIRT5 is at 34 kDa.)

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SIRT5, Polyclonal Antibody (Cat# AAA126222)

Full Name

Anti-SIRT5 Antibody Picoband

Gene Names

SIRT5; SIR2L5

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of ETV3 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ETV3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human ETV3 in human esophagus with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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ETV3, Polyclonal Antibody (Cat# AAA256551)

Full Name

Anti-ETV3 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervical cancer tissue using KCNA1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of U251 cell line, using KCNA1 antibody.)

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KCNA1, Polyclonal Antibody (Cat# AAA301593)

Full Name

KCNA1 antibody

Gene Names

KCNA1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Hela cells, Primary antibody: AAA241279(IPO9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA241279(IPO9 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241279(IPO9 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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IPO9, Polyclonal Antibody (Cat# AAA241279)

Full Name

IPO9 Antibody

Gene Names

IPO9; Imp9

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human colon cancer tissue, Primary antibody: AAA240504(PRKAR1B Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA240504(PRKAR1B Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA240504(PRKAR1B Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PRKAR1B, Polyclonal Antibody (Cat# AAA240504)

Full Name

PRKAR1B Antibody

Gene Names

PRKAR1B; PRKAR1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of TSSK4 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (TSSK4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSSK4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of TSSK4 (arrow) using rabbit polyclonal TSSK4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSSK4 gene.)

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TSSK4, Polyclonal Antibody (Cat# AAA288944)

Full Name

TSSK4 Antibody (C-term)

Gene Names

TSSK4; TSK4; TSK-4; TSSK5; STK22E; C14orf20

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/15.)

Application Data (Gel: 8%SDS-PAGE Lysate: 40ug Hela cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

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ACADVL, Polyclonal Antibody (Cat# AAA307610)

Full Name

ACADVL Antibody

Gene Names

ACADVL; ACAD6; LCACD; VLCAD

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA241490(CYBB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241490(CYBB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CYBB, Polyclonal Antibody (Cat# AAA241490)

Full Name

CYBB Antibody

Gene Names

CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: 293T cells, hepG2 cells, A549 cells, Primary antibody: AAA240976(CKAP4 Antibody) at dilution 1/1250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240976(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA240976(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CKAP4, Polyclonal Antibody (Cat# AAA240976)

Full Name

CKAP4 Antibody

Gene Names

CKAP4; p63; CLIMP-63; ERGIC-63

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MAP1A Polyclonal Antibody at dilution 1:25)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using MAP1A Polyclonal Antibody at dilution 1:25)

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MAP1A, Polyclonal Antibody (Cat# AAA168896)

Full Name

MAP1A Polyclonal Antibody

Gene Names

MAP1A; MAP1L; MTAP1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant CGa, Human.)

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Chorionic Gonadotropin Alpha Polypeptide (CGa), Polyclonal Antibody (Cat# AAA133427)

Full Name

Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa)

Gene Names

CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohistochemistry) (FITC staining on IHC-P Simple: Hela cells))

IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant CASP8, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human HepG2 Cells; Lane3: Mouse RAW264.7 Cells; Lane4: Mouse Spleen Tissue.)

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Caspase 8 (CASP8), Polyclonal Antibody (Cat# AAA133248)

Full Name

Polyclonal Antibody to Caspase 8 (CASP8)

Gene Names

CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$200/0.1 mL $235/0.2 mL $370/0.5 mL $440/1 mL $1,180/5 mL $2,295/2x5 mL $3,355/3x5 mL $4,355/4x5 mL $5,320/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type BXPC3 cell lysate; Lane 2: S100A2 knockout BXPC3 cell lysate; Predicted MW: 11kd Observed MW: 10kd Primary Ab: 3ug/ml Rabbit Anti-Human S100A2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant S100A2, Human.)

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S100 Calcium Binding Protein A2 (S100A2), Polyclonal Antibody (Cat# AAA133177)

Full Name

Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)

Gene Names

HSPA2; HSP70-2; HSP70-3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using RAD23B Polyclonal Antibody at dilution of 1:25(×200))

WB (Western Blot) (Western blot analysis of 293T cell HUVEC cell Hela cell lysates using RAD23B Polyclonal Antibody at dilution of 1:350)

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RAD23B, Polyclonal Antibody (Cat# AAA176405)

Full Name

RAD23B Polyclonal Antibody

Gene Names

RAD23B; P58; HR23B; HHR23B

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using SMOC1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Mouse liver using SMOC1 Polyclonal Antibody at 1:1000 dilution.)

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SMOC1, Polyclonal Antibody (Cat# AAA179283)

Full Name

SMOC1 Polyclonal Antibody

Gene Names

SMOC1; OAS

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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WB (Western Blot) (Western blot analysis of OR1L4/1L6 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA323634 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using OR1L4/1L6 Antibody.)

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OR1L4/1L6, Polyclonal Antibody (Cat# AAA323634)

Full Name

OR1L4/1L6 Antibody

Gene Names

OR1L4; OR1L5; OR9-E; OR9-29; OST046

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA323701 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of TLR9 using HUVEC whole cell lysates)

IHC (Immunohistochemistry) (AAA323701 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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TLR9, Polyclonal Antibody (Cat# AAA323701)

Full Name

TLR9 Antibody

Gene Names

TLR9; CD289

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HuvEc cells, using RORG antibody.The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA323899 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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RORG, Polyclonal Antibody (Cat# AAA323899)

Full Name

RORG Antibody

Gene Names

RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using LAMB1 antibody.)

IF (Immunofluorescence) (AAA324303 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA324303 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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LAMB1, Polyclonal Antibody (Cat# AAA324303)

Full Name

LAMB1 Antibody

Gene Names

LAMB1; CLM; LIS5

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA325224 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells using TSEN54 antibody.The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA325224 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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TSEN54, Polyclonal Antibody (Cat# AAA325224)

Full Name

TSEN54 Antibody

Gene Names

TSEN54; PCH4; PCH5; PCH2A; sen54; SEN54L

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA321407 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of hela cells, using Phospho-TLK1 (Ser743) Antibody.)

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TLK1, Polyclonal Antibody (Cat# AAA321407)

Full Name

Phospho-TLK1 (Ser743) Antibody

Gene Names

TLK1; PKU-beta

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of BRCA1 phosphorylation expression in whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321610 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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BRCA1, Polyclonal Antibody (Cat# AAA321610)

Full Name

Phospho-BRCA1 (Ser1423) Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA321636 at 1/100 staining human Kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321636 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of SOX-9 phosphorylation expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321636 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-SOX-9 (Ser181) expression in various lysates)

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SOX-9, Polyclonal Antibody (Cat# AAA321636)

Full Name

Phospho-SOX-9 (Ser181) Antibody

Gene Names

SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10

Reactivity

Human, Mouse, Chicken

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321238 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemisry) (AAA321238 at 1/100 staining human Lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis on Jurkat cell lysate using TOP2A Antibody)

WB (Western Blot) (Western blot analysis on mouse muscle lysate using TOP2A Antibody)

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TOP2A, Polyclonal Antibody (Cat# AAA321238)

Full Name

TOP2A Antibody

Gene Names

TOP2A; TOP2; TP2A

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Cortactin (Tyr466) expression in various lysates)

WB (Western Blot) (Western blot analysis of Cortactin phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA321727 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA321727 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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Cortactin, Polyclonal Antibody (Cat# AAA321727)

Full Name

Phospho-Cortactin (Tyr466) Antibody

Gene Names

CTTN; EMS1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (IHC analysis of Annexin I expression in COLO.)

WB (Western Blot) (Western blot analysis of Annexin I Antibody expression in mouse liver tissue lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Annexin I expression in Mouse brain lysate)

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Annexin I, Polyclonal Antibody (Cat# AAA321831)

Full Name

Annexin I Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322003 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322003 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Gastrin expression in NIH/3T3 cell extract.The lane on the left is treated with the antigen-specific peptide.)

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Gastrin, Polyclonal Antibody (Cat# AAA322003)

Full Name

Gastrin Antibody

Gene Names

GAST; GAS

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA322296 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322296 staining PC-3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA322296 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of CHOP expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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CHOP, Polyclonal Antibody (Cat# AAA322296)

Full Name

CHOP Antibody

Gene Names

DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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Application Data

Application Data

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ANKRD33, Polyclonal Antibody (Cat# AAA109415)

Full Name

ANKRD33 Antibody

Gene Names

ANKRD33; PANKY; C12orf7

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$440/0.1 mg $710/0.1 mL (AF405M) $710/0.1 mL (AF568) $710/0.1 mL (AF555) $710/0.1 mL (AF488) $710/0.1 mL (AF350) $710/0.1 mL (FITC) $710/0.1 mL (Biotin) $710/0.1 mL (AF635) $710/0.1 mL (AF610) $710/0.1 mL (AF405S) $710/0.1 mL (AF405L) $710/0.1 mL (AF546) $710/0.1 mL (AF680) $710/0.1 mL (AF647) $710/0.1 mL (AF594) $710/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AF750) $710/0.1 mL (AP)

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Application Data

Application Data

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ZBED5, Polyclonal Antibody (Cat# AAA109172)

Full Name

ZBED5 Antibody

Gene Names

ZBED5; Buster1

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$440/0.1 mg $710/0.1 mL (AF405M) $710/0.1 mL (AF568) $710/0.1 mL (AF555) $710/0.1 mL (AF488) $710/0.1 mL (AF350) $710/0.1 mL (FITC) $710/0.1 mL (Biotin) $710/0.1 mL (AF635) $710/0.1 mL (AF610) $710/0.1 mL (AF405S) $710/0.1 mL (AF405L) $710/0.1 mL (AF546) $710/0.1 mL (AF680) $710/0.1 mL (AF647) $710/0.1 mL (AF594) $710/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AF750) $710/0.1 mL (AP)

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IHC (Immunohistochemistry) (Immunohistochemical staining of paraffin-embedded H661 xenograft using MCD antibody at a dilution of 1:500)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCD antibody at 1:200 dilution.)

WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5 % SDS PAGE gel and MCD antibody at a dilution of 1:1000)

WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 7.5 % SDS PAGE gel and MCD antibody at a dilution of 1:1000)

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MCD, Polyclonal Antibody (Cat# AAA107823)

Full Name

MCD antibody

Gene Names

MLYCD; MCD

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

MCD antibody was purified by antigen-affinity chromatography

Pricing

$1,375/0.1 mL $2,675/2x0.1 mL $3,905/3x0.1 mL $5,065/4x0.1 mL $5,950/5x0.1 mL

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IHC (Immunohiostchemistry) (Anti-Kallikrein 1 antibody, AAA45159, IHC(P)IHC(P): Rat Kidney Tissue)

WB (Western Blot) (Anti-Kallikrein 1 antibody, AAA45159, Western blottingLane 1: Rat Pancreas Tissue LysateLane 2: Rat Kidney Tissue Lysate)

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Kallikrein 1, Polyclonal Antibody (Cat# AAA45159)

Full Name

Anti-Kallikrein 1 antibody

Gene Names

Klk1; Ngfg; rK-1; rGK-1; Klk1b3; Klk1c1

Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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Application Data ((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)

IHC (Immunohiostchemistry) ((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0. 05ug/ml) staining of HeLa (nuclear) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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NANOG, Polyclonal Antibody (Cat# AAA60948)

Full Name

Goat anti-NANOG (C Terminus) Antibody

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) (Sample (30 ug of whole cell lysate). A: Hep G2. B: Raji. 10% SDS PAGE. GNAS antibody diluted at 1:1000.)

IHC (Immunohiostchemistry) (Anti-GNAS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-GNAS antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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GNAS, Polyclonal Antibody (Cat# AAA52238)

Full Name

Rabbit Polyclonal to Human GNAS

Gene Names

GNAS; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (DBH antibody (2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-DBH/Dopamine Beta Hydroxylase antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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DBH/Dopamine Beta Hydroxylase, Polyclonal Antibody (Cat# AAA52340)

Full Name

Goat Polyclonal to Human DBH/Dopamine Beta Hydroxylase

Gene Names

DBH; DBM

Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Rabbit, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using IKZF1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of BT-474 cells, using IKZF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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IKZF1, Polyclonal Antibody (Cat# AAA37313)

Full Name

IKZF1 Polyclonal Antibody

Gene Names

IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NR0B2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NR0B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NR0B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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NR0B2, Polyclonal Antibody (Cat# AAA37282)

Full Name

NR0B2 Polyclonal Antibody

Gene Names

NR0B2; SHP; SHP1

Applications

Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SMARCA5 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SMARCA5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2min.)

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SMARCA5, Polyclonal Antibody (Cat# AAA37510)

Full Name

SMARCA5 Polyclonal Antibody

Gene Names

SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135

Reactivity

Human, Mouse

Applications

Chromatin immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 20 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of IRAK2 in HeLa cells with IRAK antibody at 10 μg/mL.)

WB (Western Blot) (Western blot analysis of IRAK2 in K562 whole cell lysate with IRAK2 antibody at 1:500 dilution.)

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IRAK-2, Polyclonal Antibody (Cat# AAA40081)

Full Name

IRAK-2 Antibody

Gene Names

IRAK2; IRAK-2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

IRAK-2 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of MATN1 in Human Liver cells with MATN1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of MATN1 in human liver tissue with MATN1 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of MATN1 in rat liver tissue lysate with MATN1 antibody at (A) 1 and (B) 2 μg/mL.)

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MATN1, Polyclonal Antibody (Cat# AAA39700)

Full Name

MATN1 Antibody

Gene Names

MATN1; CMP; CRTM

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

MATN1 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA240042(LRP2 Antibody) at dilution 1/16, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA240042(LRP2 Antibody) at dilution 1/16, on the right is treated with synthetic peptide. (Original magnification: ×200))

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LRP2, Polyclonal Antibody (Cat# AAA240042)

Full Name

LRP2 Antibody

Gene Names

LRP2; DBS; GP330

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Huvec cells, Primary antibody: AAA241874(TPH2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241874(TPH2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA241874(TPH2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

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TPH2, Polyclonal Antibody (Cat# AAA241874)

Full Name

TPH2 Antibody

Gene Names

TPH2; NTPH; ADHD7

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.