At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 5250-5300 of 98831 product results
WB (Western Blot) ((2. 0ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IF (Immunofluorescence) (Immunofluorescence of LRRTM4 in human brain tissue with LRRTM4 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LRRTM4 in human brain tissue with LRRTM4 antibody at 2.5 μg/mL.)
WB (Western Blot) (Western blot analysis of LRRTM4 in HeLa cell lysate with LRRTM4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
IF (Immunofluorescence) (Immunofluorescence of UNG2 in 3T3 cells with UNG2 antibody at 20 μg/mL.)
WB (Western Blot) (Western blot analysis of UNG2 in 3T3 cell lysate with UNG2 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.)
IHC (Immunohiostchemistry) (Anti-Kallikrein 1 antibody, AAA44970, IHC(P)IHC(P): Human Pancreatic Cancer Tissue)
WB (Western Blot) (Anti-Kallikrein 1 antibody, AAA44970, Western blottingLane 1: Recombinant Human KLK1 Protein 10ngLane 2: Recombinant Human KLK1 Protein 5ngLane 3: Recombinant Human KLK1 Protein 2.5ng)
WB (Western Blot) (Western blot analysis of extracts from 293 cells (Lane 2), using Potassium Channel Kv3.2b antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using Potassium Channel Kv3.2b antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Potassium Channel Kv3.2b antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Ab-31) Antibody(left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from 293 cells using Paxillin(Ab-31) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human Skeletal Muscle tissue reacted with JMJD3 Antibody(C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of anti-JMJD3 C-term Pab in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.)
IHC (Immunohiostchemistry) (Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 ug/mL.)
WB (Western Blot) (Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA241512(OSM Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AAA241512(OSM Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ARL2BP Polyclonal Antibody at dilution of 1:50(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ARL2BP Polyclonal Antibody at dilution of 1:50(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat stomach using TRPV6 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using TRPV6 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using STK32C antibody.The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA325114 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA325114 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using STK32C antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using KCNK6 antibody.)
IF (Immunofluorescence) (AAA324967 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence) (AAA320870 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis on HeLa cell lysate using p14 ARF Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA320870 at 1/200 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (IHC analysis of human kidney, using APLF Antibody at 1/100.)
WB (Western Blot) (Western blot analysis on rat heart cell lysate using APLF Antibody.The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA322303 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of BRCA1 expression in serum treated COLO205 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) ((1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
Western Blot, Peptide ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot) ((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IF (Immunofluorescence) (Staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
WB (Western Blot) ((1ug/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1ug/ml) negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using ATG7 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using ATG7 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using ATG7 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human testis tissue, mouse brain tissue, Primary antibody: AAA238296(PSMB7 Antibody) at dilution 1/310, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA238296(PSMB7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA238296(PSMB7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200))
SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: AAA240009(MAPK10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 minutes)
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA240009(MAPK10 Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240395(CR2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using AAA240395(CR2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Jurkat, A549, hepg2 and NIH/3T3 cell, Primary antibody: AAA241968(PKM Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241968(PKM Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241968(PKM Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))
IP (Immunoprecipitation) (ACTN4 was immunoprecipitated using:Lane A:0.5 mg HepG2 A431 Whole Cell Lysate4 uL anti-ACTN4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ACTN4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 95 kDaObserved band size :100 kDa)
IHC (Immunohistochemisry) (Immunochemical staining of human ACTN4 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry) (Immunochemical staining of human ACTN4 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-ACTN4 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A431 Whole Cell LysateLane C: MCF-7 Whole Cell LysateLane D: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:105 kDaObserved band size:105 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr69 or 51) Antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr69 or 51) Antibody(left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2(Phospho-Thr69 or 51) Antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: AAA240908(CTSC Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using AAA240908(CTSC Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Ab-232) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from 293 cells using G3BP-1(Ab-232) Antibody and the same antibody preincubated with blocking peptide.)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1197) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Tyr1197) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
IHC (Immunohistochemisry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TUBB Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TUBB Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
SDS-PAGE (Gel: 8%SDS-PAGE Lysate: 40 ug, Lane 1-5: NIH/3T3£¬HEPG2£¬Hela and 293T cell£¬Mouse brain tissue lysates, Primary antibody:TUBB antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Polyclonal Antibody to Phospholipase A2 Activating Protein (PLAA)
Gene Names
PLAA; DOA1; PLAP; PLA2P; NDMSBA
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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