Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using Prostate-specific Antigen antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse heart, using Prostate-specific Antigen antibody.)

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KLK3, Polyclonal Antibody (Cat# AAA242328)

Full Name

KLK3 Antibody

Gene Names

KLK3; APS; PSA; hK3; KLK2A1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA241327(KISS1R Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using AAA241327(KISS1R Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

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KISS1R, Polyclonal Antibody (Cat# AAA241327)

Full Name

KISS1R Antibody

Gene Names

KISS1R; HH8; GPR54; AXOR12; KISS-1R; HOT7T175

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of Hela A549 cells using Tubulin a Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of MCF7 cells using Tubulin a Polyclonal Antibody)

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Tubulin alpha, Polyclonal Antibody (Cat# AAA304555)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/40.)

Application Data (Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell, mouse liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 6 minutes)

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TDP2, Polyclonal Antibody (Cat# AAA305597)

Full Name

TDP2 Antibody

Gene Names

TDP2; EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast caricnoma using PR Monoclonal Antibody.)

WB (Western Blot) (Western Blot analysis of VEC cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody)

Application Data (1) Input: Mouse Brain Tissue Lysate2) IP product: IP dilute 1:200)

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Cleaved-Plasma Kallikrein HC, Polyclonal Antibody (Cat# AAA305444)

Full Name

Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody

Gene Names

KLKB1; PPK; KLK3; PKKD

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Serum; Primary Ab: 2ug/ml Rabbit Anti-Mouse ITGaM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Integrin Alpha M (ITGaM), Polyclonal Antibody (Cat# AAA132327)

Full Name

Polyclonal Antibody to Integrin Alpha M (ITGaM)

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Reactivity

Mouse, Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

WB (Western Blot) ((Figure. Western Blot; Sample: Recombinant protein.))

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Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Polyclonal Antibody (Cat# AAA132442)

Full Name

Biotin-Linked Antibody to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)

Gene Names

TIMP1; EPA; EPO; HCI; CLGI; TIMP

Reactivity

Mouse

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific Affinity Chromatography

Pricing

$195/0.1 mL $220/0.2 mL $355/0.5 mL $410/1 mL $1,095/5 mL $2,125/2x5 mL $3,105/3x5 mL $4,025/4x5 mL $4,910/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Mouse Brain Tissue.)

IHC (Immunohiostchemistry) (DAB staining on IHC-P; Samples: Mouse Spleen Tissue)

WB (Western Blot) (Western Blot: Sample: Recombinant LAMa1, Mouse.)

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Laminin Alpha 1 (LAMa1), Polyclonal Antibody (Cat# AAA133483)

Full Name

Polyclonal Antibody to Laminin Alpha 1 (LAMa1)

Gene Names

Lama1; Lama; AA408497

Reactivity

Mouse, Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $265/0.2 mL $415/0.5 mL $500/1 mL $1,360/5 mL $2,645/2x5 mL $3,860/3x5 mL $5,010/4x5 mL $6,080/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Rat Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Adenylyl Cyclase Associated Protein 1 (CAP1), Polyclonal Antibody (Cat# AAA133514)

Full Name

Polyclonal Antibody to Adenylyl Cyclase Associated Protein 1 (CAP1)

Gene Names

CAP1; CAP; CAP1-PEN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded adrenal tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant SIRT5, Mouse.)

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Sirtuin 5 (SIRT5), Polyclonal Antibody (Cat# AAA133419)

Full Name

Polyclonal Antibody to Sirtuin 5 (SIRT5)

Gene Names

Sirt5; AV001953; 0610012J09Rik; 1500032M05Rik

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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WB (Western Blot) (WB Suggested Anti-Pax7 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Rat Muscle)

IF (Immunofluorescence) (Sample Type : C2C12)

IF (Immunofluorescence) (Immunofluorescence -- Sample Type: Overexpression of Pax7 in C2C12 cellsDilution: 1:100)

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Pax7, Polyclonal Antibody (Cat# AAA197120)

Full Name

Pax7 antibody - middle region

Gene Names

Pax7; RGD1564360

Reactivity

Cow, Dog, Goat, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-CIT antibody (AAA128235).Overlay histogram showing SiHa cells stained with AAA128235 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIT Antibody (AAA128235, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of CIT using anti-CIT antibody (AAA128235).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIT antigen affinity purified polyclonal antibody (AAA128235) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CIT at approximately 231 kDa. The expected band size for CIT is at 231 kDa.)

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CIT, Polyclonal Antibody (Cat# AAA128235)

Full Name

Anti-CIT Antibody Picoband

Gene Names

CIT; CRIK; STK21

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Biotin) $705/0.1 mg (HRP) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (SNX24 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNX24 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (SNX24 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SNX24 antibody detected the SNX24 protein (arrow).)

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SNX24, Polyclonal Antibody (Cat# AAA288325)

Full Name

SNX24 Antibody (Center)

Gene Names

SNX24; SBBI31; PRO1284

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (TSPYL6 Antibody (Center) western blot analysis in NCI-H460,Hela cell line lysates (35ug/lane).This demonstrates the TSPYL6 antibody detected the TSPYL6 protein (arrow).)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of TSPYL6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry) (TSPYL6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (TSPYL6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSPYL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

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TSPYL6, Polyclonal Antibody (Cat# AAA288278)

Full Name

TSPYL6 Antibody (Center)

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using NDUFV3 antibody.)

WB (Western Blot) (Western blot analysis of lysate from human heart tissue lysate,using NDUFV3 Antibody(AAA289964). AAA289964 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)

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NDUFV3, Polyclonal Antibody (Cat# AAA289964)

Full Name

NDUFV3 Antibody

Gene Names

NDUFV3; CI-10k; CI-9KD

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM/FACS (Flow Cytometry) (HSPA6 Antibody (C-term) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody (C-term). AAA288648 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)

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HSPA6, Polyclonal Antibody (Cat# AAA288648)

Full Name

HSPA6 Antibody (C-term)

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat and COLO205 cells, using IRF2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using IRF2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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IRF2, Polyclonal Antibody (Cat# AAA317437)

Full Name

IRF2 Antibody

Gene Names

IRF2; IRF-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using EFNA5 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EFNA5 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using EFNA5 Antibody. The picture on the right is blocked with the synthesized peptide.)

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EFNA5, Polyclonal Antibody (Cat# AAA317499)

Full Name

EFNA5 Antibody

Gene Names

EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (AAA323708 staining SW626 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-beta-Catenin (Ser33/37/Thr41) using HepG2 whole cell lysates)

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beta-Catenin, Polyclonal Antibody (Cat# AAA323708)

Full Name

Phospho-beta-Catenin (Ser33/37/Thr41) Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Ep-CAM Rabbit Polyclonal Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Ep-CAM Rabbit Polyclonal Antibody.)

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Ep-CAM/CD326, Polyclonal Antibody (Cat# AAA214441)

Full Name

Ep-CAM/CD326 (Epithelial Marker)

Gene Names

EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Reactivity

Human.
Does not react with Mouse and Rat. Others not known.

Applications

Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (With BSA & Azide at 0.2mg/mL) $500/0.1 mg (Without BSA & Azide at 1mg/mL) $2,080/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $2,080/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Western blot analysis of extracts of rat testis tissue, using DCTN6 antibody.)

IHC (Immunohistochemistry) (AAA323205 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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DCTN6, Polyclonal Antibody (Cat# AAA323205)

Full Name

DCTN6 Antibody

Gene Names

DCTN6; WS3; p27; WS-3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of 231 cells using THAP3 Polyclonal Antibody at dilution of 1/200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using THAP3 Polyclonal Antibody at dilution of 1/20)

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THAP3, Polyclonal Antibody (Cat# AAA173262)

Full Name

THAP3 Polyclonal Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human placenta using AGT Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using AGT Polyclonal Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells using AGT Polyclonal Antibody at dilution of 1:1000.)

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AGT, Polyclonal Antibody (Cat# AAA177279)

Full Name

AGT Polyclonal Antibody

Gene Names

AGT; ANHU; SERPINA8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using KLK5 Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using KLK5 Polyclonal Antibody at dilution of 1:200 (40x lens).)

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KLK5, Polyclonal Antibody (Cat# AAA177612)

Full Name

KLK5 Polyclonal Antibody

Gene Names

KLK5; SCTE; KLKL2; KLK-L2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of Hela cells using anti-RPB5/POLR2E antibody (AAA127544).Overlay histogram showing Hela cells stained with AAA127544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPB5/POLR2E Antibody (AAA127544, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (AAA127544) and anti-Beta Tubulin antibody (M01857-3).RPB5/POLR2E was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-RPB5/POLR2E Antibody (AAA127544) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (AAA127544).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPB5/POLR2E antigen affinity purified polyclonal antibody (#AAA127544) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPB5/POLR2E at approximately 23 kDa. The expected band size for RPB5/POLR2E is at 23-25 kDa.)

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RPB5/POLR2E, Polyclonal Antibody (Cat# AAA127544)

Full Name

Anti-RPB5/POLR2E Antibody Picoband

Gene Names

POLR2E; RPB5; XAP4; RPABC1; hRPB25; hsRPB5

Reactivity

Human, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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WB (Western Blot) (Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. FASLG antibody diluted at 1:1000.)

IHC (Immunohiostchemistry) (Anti-FASLG / FASL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry) (Anti-FASLG / FASL antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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FASLG / Fas Ligand, Polyclonal Antibody (Cat# AAA51522)

Full Name

Rabbit Polyclonal to Human FASLG / Fas Ligand

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Western blot analysis of SIGLEC15 in K562 cell lysate with SIGLEC15 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

IHC (Immunohistochemistry) (Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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SIGLEC15 / SIGLEC-15, Polyclonal Antibody (Cat# AAA52442)

Full Name

Rabbit Polyclonal to Human SIGLEC15 / SIGLEC-15

Gene Names

SIGLEC15; CD33L3; HsT1361; SIGLEC-15

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Peptide Column Affinity Chromatography

Pricing

$765/0.05 mg $1,495/2x0.05 mg $2,185/3x0.05 mg $2,835/4x0.05 mg $3,235/5x0.05 mg

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WB (Western Blot) (Western blot analysis of lysates from HeLa and MCF-7 cells, using CHRM2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using CHRM2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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CHRM2, Polyclonal Antibody (Cat# AAA318070)

Full Name

CHRM2 Antibody

Gene Names

CHRM2; HM2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using GRM7 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using GRM7 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GRM7, Polyclonal Antibody (Cat# AAA318124)

Full Name

GRM7 Antibody

Gene Names

GRM7; GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of PAI-2 antibody in paraffin-embedded human brain tissue.)

WB (Western Blot) (Western blot analysis of lysate from MCF-7 cells, using PAI-2 antibody.)

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PAI-2, Polyclonal Antibody (Cat# AAA318476)

Full Name

PAI-2 Antibody

Gene Names

SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (AAA322301 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Synuclein expression in HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Synuclein, Polyclonal Antibody (Cat# AAA322301)

Full Name

Synuclein Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from COLO cells, using PKR1 antibody.)

IF (Immunofluorescence) (AAA325745 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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PKR1, Polyclonal Antibody (Cat# AAA325745)

Full Name

PKR1 Antibody

Gene Names

PROKR1; ZAQ; PKR1; GPR73; PK-R1; GPR73a

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) ((2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0. 01ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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PEBP1/RKIP, Polyclonal Antibody (Cat# AAA61077)

Full Name

Goat anti-PEBP1/RKIP (Internal) Antibody

Gene Names

PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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Application Data (PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. DyLight-488 Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 10ug/mL.)

IHC (Immunohistochemistry) (PCNA was detected in immunohistochemistry sections of HELA cell using mouse anti- PCNA Antigen Affinity purified monoclonal antibody at 1 ug/mL. DyLight 488 Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 10 ug/mL.)

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DyLight488 Conjugated Goat Anti-mouse IgG, Polyclonal Secondary Antibody (Cat# AAA46013)

Full Name

DyLight488 Conjugated Goat Anti-mouse IgG

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry

Pricing

$275/0.5 mL $385/1 mL $745/2x1 mL $1,090/3x1 mL $1,410/4x1 mL $1,530/5x1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HSP40 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of HSP40 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of HSP40 expression in HEK293T (A), HeLa (B), NIH3T3 (C), PC12 (D), Jurkat (E), HepG2 (F) whole cell lysates.)

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HSP40, Polyclonal Antibody (Cat# AAA105109)

Full Name

Anti-HSP40 Antibody

Gene Names

DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B

Reactivity

Human, Mouse, Rat, Bovine, Dog, Monkey, Pig

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Cytokeratin 19 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Cytokeratin 19 expression in MCF7 (A), A431 (B), HeLa (C), HT29 (D), HepG2 (E) whole cell lysates.)

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Cytokeratin 19, Polyclonal Antibody (Cat# AAA104451)

Full Name

Anti-Cytokeratin 19 Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241503(SH2D3A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA241503(SH2D3A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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SH2D3A, Polyclonal Antibody (Cat# AAA241503)

Full Name

SH2D3A Antibody

Gene Names

SH2D3A; NSP1

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal brain tissue, Primary antibody: AAA241726(SLC16A8 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241726(SLC16A8 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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SLC16A8, Polyclonal Antibody (Cat# AAA241726)

Full Name

SLC16A8 Antibody

Gene Names

SLC16A8; MCT3; REMP

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using KAZN Polyclonal Antibody at dilution of 1:35(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using KAZN Polyclonal Antibody at dilution of 1:35(×200))

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KAZN, Polyclonal Antibody (Cat# AAA176189)

Full Name

KAZN Polyclonal Antibody

Gene Names

KAZN; KAZ; C1orf196

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HepG2 cells using anti-ZNF609 antibody (AAA126641).Overlay histogram showing HepG2 cells stained with AAA126641 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNF609 Antibody (AAA126641, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of ZNF609 using anti-ZNF609 antibody (AAA126641).ZNF609 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-ZNF609 Antibody (AAA126641) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of ZNF609 using anti-ZNF609 antibody (AAA126641).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF609 antigen affinity purified polyclonal antibody (#AAA126641) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZNF609 at approximately 180 kDa. The expected band size for ZNF609 is at 151 kDa.)

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ZNF609, Polyclonal Antibody (Cat# AAA126641)

Full Name

Anti-ZNF609 Antibody Picoband

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-OLFML2B antibody (AAA127892).Overlay histogram showing SiHa cells stained with AAA127892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLFML2B Antibody (AAA127892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of OLFML2B using anti-OLFML2B antibody (AAA127892).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (#AAA127892) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.)

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OLFML2B, Polyclonal Antibody (Cat# AAA127892)

Full Name

Anti-OLFML2B Antibody Picoband

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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Application Data (Immunocytochemical staining of HL60 cells with Rabbit anti Human adiponectin (AAA49910))

Application Data (Immunohistochemical staining of rat brain with Rabbit anti Human adiponectin (AAA49910))

Application Data (Western blot analysis of hole cell lysate from HL60 promyelocytic leukemia cells probed with Rabbit anti Human adiponectin (AAA49910) at 0.5(A), 1(B) and 2(C) ug/ml)

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ADIPONECTIN, Polyclonal Antibody (Cat# AAA49910)

Full Name

RABBIT ANTI ADIPONECTIN (C-TERMINAL)

Gene Names

ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$680/0.1 mg $1,325/2x0.1 mg $1,930/3x0.1 mg $2,505/4x0.1 mg $2,840/5x0.1 mg

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WB (Western Blot) (Western blot of extracts from HT-29 cells, using DNMT3B Antibody. The lane on the right is treated with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence of HeLa cells, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Anti-DNMT3B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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DNMT3B, Polyclonal Antibody (Cat# AAA51269)

Full Name

Rabbit Polyclonal (IgG) to Human DNMT3B

Gene Names

DNMT3B; ICF; ICF1; M.HsaIIIB

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Antibody (0.1 ug/ml) staining of Rat Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-OGG1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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OGG1, Polyclonal Antibody (Cat# AAA50872)

Full Name

Goat Polyclonal to Mouse OGG1

Gene Names

OGG1; HMMH; MUTM; OGH1; HOGG1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Toll Like Receptor 1 (TLR1), Polyclonal Antibody (Cat# AAA131618)

Full Name

Polyclonal Antibody to Toll Like Receptor 1 (TLR1)

Gene Names

TLR1; TIL; CD281; rsc786; TIL. LPRS5

Reactivity

Human, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human AGMAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Agmatine Ureohydrolase (AGMAT), Polyclonal Antibody (Cat# AAA132550)

Full Name

Polyclonal Antibody to Agmatine Ureohydrolase (AGMAT)

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cell using TK1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TK1 antibody.)

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TK1, Polyclonal Antibody (Cat# AAA166340)

Full Name

TK1 Antibody

Gene Names

TK1; TK2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (AAA61724 (0. 03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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CDH13/H cadherin, Polyclonal Antibody (Cat# AAA61724)

Full Name

Goat anti-CDH13/H cadherin (aa285-297) Antibody

Gene Names

CDH13; CDHH; P105

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.