At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (Western blot of extracts from HUVEC cells, treated with PMA 125 ng/ml 30', using PKD1/PKC mu antibody. The lane on the right is treated with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence of A549 cells, using PKD1/PKC mu antibody. The picture on the right is treated with the synthesized peptide.)
IHC (Immunohistochemistry) (Anti-PKC Mu antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)
IF (Immunofluorescence) (Immunofluorescence of RTP801 in mouse kidney tissue with RTP801 antibody at 20 μg/mL.Red: RTP801 Antibody (4495)Blue: DAPI staining)
IHC (Immunohiostchemistry) (Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.)
WB (Western Blot) (Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
IHC (Immunohiostchemistry) (Fig: 2 Immunohistochemical analysis of cIAP1 in formalin-fixed, paraffin-embedded human prostate using at 1: 2000. A, normal prostate. B, prostate intraepithelial neoplasia (PIN). PIN is a premalignant proliferation arising within the prostate. C, prostate cancer. Hematoxylin-eosin counterstain. Increased cIAP1 expression is PIN and in prostate cancer compared to normal prostate.)
WB (Western Blot) (Fig: 1 Western blot analysis of cIAP1 in human prostate using at 1: 1000. PBH (benign prostate hyperplasia), PIN (prostate intraepithelial neoplasia). Elevated levels of cIAP1 were seen in certain cases of BPH/PIN and prostate cancer compared to normal prostate.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of 293T cells using anti-PDIP/PDIA2 antibody (AAA126295).Overlay histogram showing 293T cells stained with AAA126295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIP/PDIA2 Antibody (AAA126295, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of PDIP/PDIA2 using anti-PDIP/PDIA2 antibody (AAA126295).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human PCP tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIP/PDIA2 antigen affinity purified polyclonal antibody (#AAA126295) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDIP/PDIA2 at approximately 65 kDa. The expected band size for PDIP/PDIA2 is at 68 kDa.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Triadin/TRDN antibody (AAA126474).Overlay histogram showing MCF-7 cells stained with AAA126474 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Triadin/TRDN Antibody (AAA126474, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of Triadin/TRDN using anti-Triadin/TRDN antibody (AAA126474).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Triadin/TRDN antigen affinity purified polyclonal antibody (#AAA126474) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Triadin/TRDN at approximately 25 kDa. The expected band size for Triadin/TRDN is at 82 kDa.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CBS antibody (AAA125963).Overlay histogram showing CACO-2 cells stained with AAA125963 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBS Antibody (AAA125963, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 2. IF analysis of CBS using anti-CBS antibody (AAA125963).CBS was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CBS Antibody (AAA125963) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot) (Figure 1. Western blot analysis of CBS using anti-CBS antibody (AAA125963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: huamn HEK293 whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human K562 whole cell lysates,Lane 6: rat liver tissue lysates,Lane 7: rat pancreas tissue lysates,Lane 8: mouse liver tissue lysates,Lane 9: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (#AAA125963) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CBS at approximately 63 kDa. The expected band size for CBS is at 63 kDa.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using GANC Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GANC Polyclonal Antibody at dilution of 1:55(×200))
WB (Western Blot) (Western blot analysis of TM4 cell lysate using GANC Polyclonal Antibody at dilution of 1:550)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/40.)
Application Data (Gel: 6%SDS-PAGE Lysates (from left to right): PC3, Hela, NIH/3T3 and HUVEB cell Amount of lysate: 40ug per lane Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
IF (Immunofluorescence) (Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using BIVM antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Hepatoma, using BIVM antibody at 1: 500 dilution.)
Application Data (Sample(30 ug whole cell lysate)A: H1299B: HeLa S3C: Hep G2 7.5% SDS PAGEPrimary antibody diluted at 1: 1000)
IP (Immunoprecipitation) (STX12 was immunoprecipitated using:Lane A:0.5 mg Jurkat Hela Whole Cell Lysate4 uL anti-STX12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STX12 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size :38 kDa)
WB (Western Blot) (Anti-STX12 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohiostchemistry) (Immunochemical staining of human NIN in human corpus uteri with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human NIN in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PTP4A1 Antibody at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200))
SDS-PAGE (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane: HEPG2 cell lysate, Primary antibody:PTP4A1 antibody at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
WB (Western Blot) (RTN1 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the RTN1 antibody detected the RTN1 protein (arrow).)
WB (Western Blot) (RTN1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RTN1 antibody detected the RTN1 protein (arrow).)
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PTGS1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence) (Fluorescent image of Hela cell stained with PTGS1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTGS1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PTGS1 immunoreactivity is localized to Golgi significantly.)
WB (Western Blot) (PTGS1 Antibody (C-term) western blot analysis in A431,K562 cell line lysates (35ug/lane).This demonstrates the PTGS1 antibody detected the PTGS1 protein (arrow).)
IHC (Immunohiostchemistry) (AAA325392 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from 293 cells, using Cytochrome P450 8B1 antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of GPR54 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA323572 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using Potassium Channel Kv3.2b Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Potassium Channel Kv3.2b Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using LGR6 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using LGR6 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from mouse brain, using Dynamin-1 (Phospho-Ser774) Antibody. The lane on the right is blocked with the phospho peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Dynamin-1 (Phospho-Ser774) Antibody. The picture on the right is blocked with the phospho peptide.)
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
WB (Western Blot) (Western blot analysis on HeLa cell lysate using Parkin Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA320876 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241964(SYNCRIP Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA241964(SYNCRIP Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BACH1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse liver using BACH1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast cancer using BACH1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat ovary using BACH1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using BACH1 Polyclonal Antibody at dilution of 1:3000.)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse pancreas using PNLIPRP2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat pancreas using PNLIPRP2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of Rat pancreas using PNLIPRP2 Polyclonal Antibody at dilution of 1:3000.)
IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of A-431 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using DBT Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using DBT Polyclonal Antibody at 1:1000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of mouse retina cells using CRX Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of Mouse eye using CRX Polyclonal Antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Anti-GPR83 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohistochemistry) (Anti-GPR83 antibody IHC of human brain, neurons and neuropil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Human Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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