Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry) (DAB staining on IHC-P;Samples: Porcine Cardiac Muscle Tissue;Primary Ab: 20ug/ml Rabbit Anti-Porcine NPPB AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot:Sample: Recombinant BNP, Porcine.)

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Brain Natriuretic Peptide (BNP), Polyclonal Antibody (Cat# AAA132596)

Full Name

Polyclonal Antibody to Brain Natriuretic Peptide (BNP)

Gene Names

NPPB; BNP; BNP1

Reactivity

Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$225/0.1 mL $290/0.2 mL $465/0.5 mL $580/1 mL $1,560/5 mL $3,035/2x5 mL $4,440/3x5 mL $5,760/4x5 mL $7,055/5x5 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using PPP1R13L Polyclonal Antibody at dilution of 1:30)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain using PPP1R13L Polyclonal Antibody at dilution of 1:30)

WB (Western Blot) (Western Blot analysis of Lncap and hela cell using PPP1R13L Polyclonal Antibody at dilution of 1:350)

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PPP1R13L, Polyclonal Antibody (Cat# AAA167492)

Full Name

PPP1R13L Polyclonal Antibody

Gene Names

PPP1R13L; RAI; RAI4; IASPP; NKIP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using FGFRL1 Polyclonal Antibody at dilution 1:20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer tissue using FGFRL1 Polyclonal Antibody at dilution 1:20)

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FGFRL1, Polyclonal Antibody (Cat# AAA167273)

Full Name

FGFRL1 Polyclonal Antibody

Gene Names

FGFRL1; FHFR; FGFR5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human gastic cancer using ABCG1 Polyclonal Antibody at dilution of 1:40)

WB (Western Blot) (Western Blot analysis of HT-29 cell using ABCG1 Polyclonal Antibody at dilution of 1:500)

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ABCG1, Polyclonal Antibody (Cat# AAA170597)

Full Name

ABCG1 Polyclonal Antibody

Gene Names

ABCG1; ABC8; WHITE1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer using TPD52L2 Polyclonal Antibody at dilution of 1:20)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using TPD52L2 Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of Mouse bladder tissue, K562 and MCF7 cell using TPD52L2 Polyclonal Antibody at dilution of 1:250)

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TPD52L2, Polyclonal Antibody (Cat# AAA170616)

Full Name

TPD52L2 Polyclonal Antibody

Gene Names

TPD52L2; D54; TPD54

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using SDF4 Polyclonal Antibody at dilution 1:40)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SDF4 Polyclonal Antibody at dilution 1:40)

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SDF4, Polyclonal Antibody (Cat# AAA173018)

Full Name

SDF4 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using TNFSF4 Polyclonal Antibody at dilution 1:20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TNFSF4 Polyclonal Antibody at dilution 1:20)

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TNFSF4, Polyclonal Antibody (Cat# AAA173229)

Full Name

TNFSF4 Polyclonal Antibody

Gene Names

TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IF (Immunofluorescence) (AAA324133 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA324133 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from COLO cells using LASS4 antibody.)

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LASS4, Polyclonal Antibody (Cat# AAA324133)

Full Name

LASS4 Antibody

Gene Names

CERS4; Trh1; LASS4

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA323667 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis Flotillin 1 using HUVEC whole cell lysates)

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Flotillin 1, Polyclonal Antibody (Cat# AAA323667)

Full Name

Flotillin 1 Antibody

Gene Names

HAPLN1; CRT1; CRTL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from K562 cells, usingPhospho-Beclin-1 (Ser15) Antibody. Lane1 was treated withphospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

IHC (Immunohistochemistry) (AAA321307 at 1/100 staining Rat colorectal tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)

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Beclin-1, Polyclonal Antibody (Cat# AAA321307)

Full Name

Phospho-Beclin-1 (Ser15) Antibody

Gene Names

BECN1; ATG6; VPS30; beclin1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of ABCG2 expression in HT-29 cells, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321847 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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ABCG2, Polyclonal Antibody (Cat# AAA321847)

Full Name

ABCG2 Antibody

Gene Names

ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (Anti-Aquaporin 6 antibody, AAA45374, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohiostchemistry) (Anti-Aquaporin 6 antibody, AAA45374, IHC(P)IHC(P): Human Placenta Tissue)

WB (Western Blot) (Anti-Aquaporin 6 antibody, AAA45374, Western blottingLane 1: U87 Cell LysateLane 2: COLO320 Cell LysateLane 3: MCF-7 Cell Lysate)

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Aquaporin 6, Polyclonal Antibody (Cat# AAA45374)

Full Name

Anti-Aquaporin 6 antibody

Gene Names

AQP6; KID; AQP2L

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using CRBP III antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CRBP III antibody.)

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RBP5, Polyclonal Antibody (Cat# AAA242435)

Full Name

RBP5 Antibody

Gene Names

RBP5; CRBP3; CRBPIII; CRBP-III

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-alpha (Phospho-Ser102) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HuvEc cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Ser102) Antibody. The lane on the left is treated with antigen-specific peptide.)

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ESR1, Polyclonal Antibody (Cat# AAA243222)

Full Name

Phospho-ESR1 (Ser102) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image of AAA243442 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissueAll lanes: RFNG antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37 kDaObserved band size: 37 kDa)

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RFNG, Polyclonal Antibody (Cat# AAA243442)

Full Name

RFNG Antibody

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

>95%, Protein G Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embeded human prostate using at dilution of 1:100.)

WB (Western Blot) (All lanes: Fibroblast growth factor 21 antibody at 2ug/ml+A549 whole cell lysate)

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Fibroblast growth factor 21, Polyclonal Antibody (Cat# AAA308478)

Full Name

Fibroblast growth factor 21 Polyclonal Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$385/0.1 mg $745/2x0.1 mg $1,090/3x0.1 mg $1,410/4x0.1 mg $1,525/5x0.1 mg

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WB (Western Blot) (Western blot analysis of lysates from 293 cells, treated with EGF, using IKK-alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using IKK-alpha Antibody. The picture on the right is blocked with the synthesized peptide.)

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IKK-alpha, Polyclonal Antibody (Cat# AAA317074)

Full Name

IKK-alpha Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FER (Phospho-Tyr402) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using FER (Phospho-Tyr402) Antibody. The picture on the right is blocked with the phospho peptide.)

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FER, Polyclonal Antibody (Cat# AAA316611)

Full Name

FER (Phospho-Tyr402) Antibody

Gene Names

FER; TYK3; PPP1R74; p94-Fer

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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RAPSN, Polyclonal Antibody (Cat# AAA319767)

Full Name

RAPSN Antibody

Gene Names

RAPSN; FADS; CMS11; CMS4C; RAPSYN; RNF205

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$265/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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IHC (Immunohistochemisry) (AAA323114 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

IHC (Immunohiostchemistry) (AAA323114 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of MCF-7 cells, using Mast Cell Chymase antibody. The lane of the left was treated with blocking peptide.)

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Mast Cell Chymase, Polyclonal Antibody (Cat# AAA323114)

Full Name

Mast Cell Chymase Antibody

Gene Names

CMA1; CYH; MCT1; chymase

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U20S cell using UHRF1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using UHRF1 antibody.)

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UHRF1, Polyclonal Antibody (Cat# AAA169060)

Full Name

UHRF1 Antibody

Reactivity

Human

Applications

Chromatin immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human prostate cancer using USP47 Polyclonal Antibody at dilution of 1/20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using USP47 Polyclonal Antibody at dilution of 1/20)

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USP47, Polyclonal Antibody (Cat# AAA173163)

Full Name

USP47 Polyclonal Antibody

Gene Names

USP47; TRFP

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colon cancer using FARSB Polyclonal Antibody at dilution of 1:20)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using FARSB Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of A172, K562 and hela cell using FARSB Polyclonal Antibody at dilution of 1:225)

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FARSB, Polyclonal Antibody (Cat# AAA167300)

Full Name

FARSB Polyclonal Antibody

Gene Names

FARSB; FRSB; PheHB; PheRS; FARSLB; HSPC173

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using EXOSC4 Polyclonal Antibody at dilution of 1:50(×200))

WB (Western Blot) (Western blot analysis of 293T LO2 Hela and Jurkat cell lysates using EXOSC4 Polyclonal Antibody at dilution of 1:650)

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EXOSC4, Polyclonal Antibody (Cat# AAA175616)

Full Name

EXOSC4 Polyclonal Antibody

Gene Names

EXOSC4; SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal using MTMR8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Rat liver using MTMR8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using MTMR8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using MTMR8 Polyclonal Antibody at 1:1000 dilution.)

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MTMR8, Polyclonal Antibody (Cat# AAA179122)

Full Name

MTMR8 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of 293T cells using anti-NDRG4 antibody (AAA127269).Overlay histogram showing 293T cells stained with AAA127269 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDRG4 Antibody (AAA127269, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of NDRG4 using anti-NDRG4 antibody (AAA127269) and anti-Beta Tubulin antibody (M01857-3).NDRG4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-NDRG4 Antibody (AAA127269) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of NDRG4 using anti-NDRG4 antibody (AAA127269).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG4 antigen affinity purified polyclonal antibody (#AAA127269) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDRG4 at approximately 37 kDa. The expected band size for NDRG4 is at 37-45 kDa.)

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NDRG4, Polyclonal Antibody (Cat# AAA127269)

Full Name

Anti-NDRG4 Antibody Picoband

Gene Names

NDRG4; BDM1; SMAP8; SMAP-8

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Heparin Binding Epidermal Growth Factor Like Growth Factor, Polyclonal Antibody (Cat# AAA142276)

Full Name

Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)

Gene Names

Hbegf; Dtr; Dts; Hegfl; AW047313

Reactivity

Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$205/0.1 mL $235/0.2 mL $375/0.5 mL $450/1 mL $1,215/5 mL $2,370/2x5 mL $3,460/3x5 mL $4,490/4x5 mL $5,465/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of Ghrelin antibody in paraffin-embedded human lung carcinoma tissue.)

WB (Western Blot) (Western blot analysis of lysate from 293 cells, using Ghrelin antibody.)

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Ghrelin, Polyclonal Antibody (Cat# AAA318446)

Full Name

Ghrelin Antibody

Gene Names

GHRL; MTLRP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (AAA326518 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Jurkat whole cell lysates, using EIF5A Antibody. The lane on the left is treated with the antigen-specific peptide.)

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EIF5A, Polyclonal Antibody (Cat# AAA326518)

Full Name

EIF5A Antibody

Gene Names

EIF5A; EIF-5A; EIF5A1; eIF5AI

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from Mouse liver, using KRAS Ab. The lane on the left was treated with blocking peptide.)

IHC (Immunohistochemistry) (AAA326101 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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KRAS, Polyclonal Antibody (Cat# AAA326101)

Full Name

KRAS Antibody

Gene Names

KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of HeLa lysate using AMPK alpha antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326136 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA326136 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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AMPK alpha, Polyclonal Antibody (Cat# AAA326136)

Full Name

AMPK alpha Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using AMACR Antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326045 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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AMACR, Polyclonal Antibody (Cat# AAA326045)

Full Name

AMACR Antibody

Gene Names

AMACR; RM; RACE; CBAS4; P504S; AMACRD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of Mouse brain, using APLP1 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326796 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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APLP1, Polyclonal Antibody (Cat# AAA326796)

Full Name

APLP1 Antibody

Gene Names

APLP1; APLP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

WB (Western Blot) ((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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Polycystin 2/PKD2, Polyclonal Antibody (Cat# AAA61444)

Full Name

Goat anti-Polycystin 2/PKD2 Antibody

Gene Names

PKD2; PC2; PKD4; Pc-2; APKD2; TRPP2

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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DB (Dot Blot) (Histone H3 monomethyl Lys79 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys79 antibody for monomethyl lysine 79 of histone H3. Peptides corresponding to the region around lysine 79 of histone H3 were spotted onto PVDF and probed with the antibody at a dilution of either 1:5,000 (panel A) or 1:50,000 (panel B). The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Unmodified Lys79 peptide. Lane 2: Monomethyl Lys79 peptide. Lane 3: Dimethyl Lys79 peptide. Lane 4: Trimethyl Lys79 peptide.)

WB (Western Blot) (Histone H3 monomethyl Lys79 antibody tested by Western blot. HeLa acid extract (10 ug) was probed with Histone H3 monomethyl Lys79 antibody (1:1,000 dilution).)

ChIP (Chromatin Immunoprecipitation) (Histone H3 monomethyl Lys79 antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 monomethyl Lys79 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on ChIP DNA using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.)

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Histone H3K79me1, Polyclonal Antibody (Cat# AAA59839)

Full Name

Histone H3K79me1 antibody (pAb)

Reactivity

Human, Wide Range Predicted

Applications

Western Blot, Dot Blot, Chromatin immunoprecipitation

Pricing

$250/0.01 mL $725/0.2 mL $1,415/2x0.2 mL $2,070/3x0.2 mL $2,680/4x0.2 mL $3,100/5x0.2 mL

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IHC (Immunohiostchemistry) (Anti-PCSK9 antibody, AAA45461, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot) (Anti-PCSK9 antibody, AAA45461, Western blottingAll lanes: Anti PCSK9 (AAA45461) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: U87 Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugPredicted bind size: 74KDObserved bind size: 74KD)

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PCSK9, Polyclonal Antibody (Cat# AAA45461)

Full Name

Anti-PCSK9 antibody

Gene Names

PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U87 cells using anti-Gastrin/GAST antibody (AAA126323).Overlay histogram showing U87 cells stained with AAA126323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (AAA126323, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-Gastrin/GAST antibody (AAA126323).Overlay histogram showing Caco-2 cells stained with AAA126323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (AAA126323, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of Gastrin/GAST using anti-Gastrin/GAST antibody (AAA126323).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysates,Lane 2: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastrin/GAST antigen affinity purified polyclonal antibody (#AAA126323) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Gastrin/GAST at approximately 20 kDa. The expected band size for Gastrin/GAST is at 11 kDa.)

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Gastrin/GAST, Polyclonal Antibody (Cat# AAA126323)

Full Name

Anti-Gastrin/GAST Antibody Picoband

Gene Names

GAST; GAS

Reactivity

Human, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

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ERCC5, Polyclonal Antibody (Cat# AAA177333)

Full Name

ERCC5 Polyclonal Antibody

Gene Names

ERCC5; XPG; UVDR; XPGC; COFS3; ERCM2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence

Purity

Affinity purification

Pricing

$230/0.06 mL $340/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,125/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human ovary using Galectin 3 Ployclonal Antibody at dilution of 1:50.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon using Galectin 3 Ployclonal Antibody at dilution of 1:50.)

WB (Western Blot) (Western Blot analysis of 1)MCF-7, 2)NIH/3T3, 3)Raw264.7 using Galectin 3 Ployclonal Antibody at dilution of 1:1000.)

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Galectin 3, Polyclonal Antibody (Cat# AAA177046)

Full Name

Galectin 3 Polyclonal Antibody

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$175/0.02 mL $225/0.06 mL $345/0.12 mL $490/0.2 mL $955/2x0.2 mL $1,400/3x0.2 mL $1,815/4x0.2 mL $2,150/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A431 cells using anti-CDC7 antibody (AAA126121).Overlay histogram showing A431 cells stained with AAA126121 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC7 Antibody (AAA126121, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of CDC7 using anti-CDC7 antibody (AAA126121).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC7 antigen affinity purified polyclonal antibody (#AAA126121) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC7 at approximately 64 kDa. The expected band size for CDC7 is at 64 kDa.)

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CDC7, Polyclonal Antibody (Cat# AAA126121)

Full Name

Anti-CDC7 Antibody Picoband

Gene Names

CDC7; Hsk1; CDC7L1; HsCDC7; huCDC7

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using BLOC1S6 Polyclonal Antibody at dilution of 1:95(×200))

WB (Western Blot) (Western blot analysis of 293T K562 and HepG2 cell lysates using BLOC1S6 Polyclonal Antibody at dilution of 1:650)

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BLOC1S6, Polyclonal Antibody (Cat# AAA175581)

Full Name

BLOC1S6 Polyclonal Antibody

Gene Names

BLOC1S6; PA; HPS9; PLDN; BLOS6; PALLID

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HEL cells using anti-SVOPL antibody (AAA127898).Overlay histogram showing HEL cells stained with AAA127898 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SVOPL Antibody (AAA127898, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of SVOPL using anti-SVOPL antibody (AAA127898).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human MOLT-4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SVOPL antigen affinity purified polyclonal antibody (#AAA127898) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SVOPL at approximately 37 kDa. The expected band size for SVOPL is at 54,37 kDa.)

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SVOPL, Polyclonal Antibody (Cat# AAA127898)

Full Name

Anti-SVOPL Antibody Picoband

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot analysis GM130 using Jurkat whole cell lysates)

IHC (Immunohistochemistry) (AAA327218 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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GM130, Polyclonal Antibody (Cat# AAA327218)

Full Name

GM130 Antibody

Gene Names

GOLGA2; GM130

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of U251, using KCNA1antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326872 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326872 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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KCNA1, Polyclonal Antibody (Cat# AAA326872)

Full Name

KCNA1 Antibody

Gene Names

KCNA1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. diluted at 1:10000.)

WB (Western Blot) (Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody diluted at 1:5000.)

IF (Immunofluorescence) (Immunofluorescence of paraformaldehyde-fixed A549, using GAPDH antibody at 1:200 dilution.)

IHC (Immunohiostchemistry) (Anti-GAPDH antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry) (Anti-GAPDH antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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GAPDH, Polyclonal Antibody (Cat# AAA52255)

Full Name

Rabbit Polyclonal to Human GAPDH

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Drosophila, Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human liver cancer using HSPA4 Polyclonal Antibody at dilution of 1:25)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil using HSPA4 Polyclonal Antibody at dilution of 1:25)

WB (Western Blot) (Western Blot analysis of Hela, Raji, NIH/3T3, HepG2 and A375 cell using HSPA4 Polyclonal Antibody at dilution of 1:300)

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HSPA4, Polyclonal Antibody (Cat# AAA166940)

Full Name

HSPA4 Polyclonal Antibody

Gene Names

HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CD204 Polyclonal Antibody at dilution of 1:25)

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MSR1, Polyclonal Antibody (Cat# AAA166668)

Full Name

MSR1 Polyclonal Antibody

Gene Names

MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human A549 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Human PRAME Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Preferentially Expressed Antigen In Melanoma (PRAME), Polyclonal Antibody (Cat# AAA132172)

Full Name

Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)

Gene Names

PRAME; MAPE; OIP4; CT130; OIP-4

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.