At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)
WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using VAV1 (Ab-174) antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using VAV1 (Ab-174) antibody.)
IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with AAA243514 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysateAll lanes: ZFYVE9 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 157, 151, 84 kDaObserved band size: 157 kDa)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TBPL1 Polyclonal Antibody at dilution of 1:50(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TBPL1 Polyclonal Antibody at dilution of 1:50(×200))
WB (Western Blot) (Western blot analysis of Mouse brain tissue lysate using TBPL1 Polyclonal Antibody at dilution of 1:400)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ACACA Polyclonal Antibody at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37 degree C for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ACACA Polyclonal Antibody at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37 degree C for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of 293T cells using ACACA Polyclonal Antibody at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37 degree C for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using ACACA Polyclonal Antibody at dilution of 1:1000.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using UNG Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using UNG Polyclonal Antibody at dilution of 1:3000.)
WB (Western Blot) (Western blot analysis S100A9 using 293 whole cell lysates)
IHC (Immunohistochemistry) (AAA327250 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry) (OMP Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry) (OMP Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot) (OMP Antibody-OMP antibody western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohistochemisry) (TMEM47 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.)
ICC (Immunocytochemistry) (TMEM47 Antibody-Immunofluorescent analysis of Hela cells using TMEM47 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
ICC (Immunocytochemistry) (TMEM47 Antibody-Immunofluorescent analysis of U251 cells using TMEM47 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry) (AAA61109 staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH6, AP-staining.)
WB (Western Blot) ((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry) (ZDHHC21 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry) (ZDHHC21 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC21 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of ZDHHC21 Antibody (N-term) in mouse liver tissue lysates (35ug/lane).ZDHHC21 (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of lysate from SH-SY5Y cell line, using ZDHHC21 Antibody (N-term)(AAA285302). AAA285302 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
FCM/FACS (Flow Cytometry) (UPK1B Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (UPK1B Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UPK1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of UPK1B Antibody (Center) in K562 cell line lysates (35ug/lane). UPK1B (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (SELT Antibody (N-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of SELT Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). SELT (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of lysates from HepG2, HUVEC, and MCF-7 cells, using OR5M3 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using OR5M3 Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence staining of U251 cells at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohiostchemistry) (IHC image diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:200 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western blot analysis of extracts of human colon cancer tissue lysate, using NOTCH3 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326916 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FCM/FACS (Flow Cytometry) (GPX1 Antibody flow cytometry of HeLa cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot of lysates from THP-1 cell line, mouse liver and rat liver tissue (from left to right),using GPX1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
IF (Immunofluorescence) (Confocal immunofluorescence of GPX1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot) (PRDX6/Peroxiredoxin 6 Antibody-PRDX6 antibody (0.1 ug/ml) staining of Liver (A) and Heart (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) (PRDX6/Peroxiredoxin 6 Antibody-Goat Anti-peroxiredoxin 6 Antibody (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)
WB (Western Blot) (Western blot analysis of DNER using HepG2 whole cell lysates)
IHC (Immunohistochemistry) (AAA322765 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of Jurkat, using HLA-DRA antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326243 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326243 staining HEPG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.)
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
WB (Western Blot) (Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Affinity purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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