At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (PCDH20 Antibody-Western blot analysis of extracts of various cell lines, using PCDH20 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.)
IHC (Immunohiostchemistry) (Immunochemical staining of human FXR1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human FXR1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry) (Immunochemical staining of human ARSA in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry) (Immunochemical staining of human ARSA in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-ARSA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:63 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA243424 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohiostchemistry) (IHC image of AAA243424 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA243424 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry) (KCNA2 Antibody (C-term) (AAA289828)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCNA2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (KCNA2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNA2 antibody detected the KCNA2 protein (arrow).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse heart using TP53I11 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lymph node tumors using TP53I11 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat spleen using TP53I11 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using INMT Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using INMT Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using PTH1R Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using PTH1R Polyclonal Antibody at dilution of 1:55(×200))
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PTRF Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using PTRF Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using PTRF Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse pancreas using IAPP Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat pancreas using IAPP Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells using IAPP Polyclonal Antibody.)
SDS-PAGE (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hepg2 cells, k562 cells, Primary antibody: AAA241698(MORF4L1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA241698(MORF4L1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241698(MORF4L1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2(Phospho-Tyr88) Antibody(left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from Mouse heart tissue using cofilin1/cofilin2(phospho-Tyr88) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GLIS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of GLIS1 Antibody (N-term) in mouse stomach tissue lysates (35ug/lane). GLIS1 (arrow) was detected using the purified Pab.)
IF (Immunofluorescence) (AAA320880 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis on HepG2 cell lysate using PCNA Antibody, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA320880 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of human brain tissue, using CX3CL1 antibody.)
IHC (Immunohistochemistry) (AAA323200 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human prost ate cancer tissue using JPT2 Polyclonal Antibody at dilution of 1:65(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using JPT2 Polyclonal Antibody at dilution of 1:65(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using KCNN3 Polyclonal Antibody at dilution of 1:60(x200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using KCNN3 Polyclonal Antibody at dilution of 1:60(x200))
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ETFB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using ETFB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ETFB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using ETFB Polyclonal Antibody at dilution of 1:1000.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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