At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human uterine cancer using MLKL Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using MLKL Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil using MLKL Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using TTR antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TTR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 μg/ml.Green: Vinculin Antibody (7809)Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 μg/ml.)
WB (Western Blot) (Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 μg/ml.)
IP (Immunoprecipitation) (BECN1 was immunoprecipitated using:Lane A:0.5 mg Hela Jurkat Whole Cell Lysate4 uL anti-BECN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-BECN1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size :60 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of BECN1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human BECN1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot) (Anti-BECN1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A431 Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohiostchemistry) (Immunochemical staining of human ARL14EP in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human ARL14EP in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
FCM/FACS (Flow Cytometry) (CYP20A1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of CYP20A1 (arrow) using rabbit polyclonal CYP20A1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYP20A1 gene.)
IHC (Immunohistochemistry) (AAA323807 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from 293 cells, using TCF3 antibody.)
WB (Western Blot) (Western blot analysis of extracts from various samples, using TCF3 Antibody.)
IHC (Immunohistochemistry) (AAA323807 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (AAA323096 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of Ramos cells, using FAM96B antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using UBD Polyclonal Antibody at dilution of 1:50(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using UBD Polyclonal Antibody at dilution of 1:50(×200))
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using PTHLH Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using PTHLH Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using PTHLH Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using KISS1R Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human B cell lymphoma using KISS1R Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat brain using KISS1R Polyclonal Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of SW480 cells using KISS1R Polyclonal Antibody at dilution of 1:350.)
IF (Immunofluorescence) (Immunofluorescence analysis of A375 cells using KLF5 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human placenta using KLF5 Polyclonal Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Antibody (0.3 ul/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IHC (Immunohiostchemistry) (Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry) (Anti-FOXA1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
WB (Western Blot) (Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4 degree over night)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
Polyclonal Antibody to Interleukin 17 Receptor B (IL17RB)
Gene Names
IL17RB; CRL4; EVI27; IL17BR; IL17RH1
Reactivity
Human, Pig
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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