Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence staining of SEPHS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SEPHS2 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human SEPHS2 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

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SEPHS2, Polyclonal Antibody (Cat# AAA256341)

Full Name

Anti-SEPHS2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (PSMG1 was immunoprecipitated using:Lane A:0.5 mg Jurkat U-251 MG Whole Cell Lysate4 uL anti-PSMG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMG1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :27 kDa)

WB (Western Blot) (Anti-PSMG1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:28 kDa)

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PSMG1, Polyclonal Antibody (Cat# AAA256250)

Full Name

Anti-PSMG1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (TMED2 was immunoprecipitated using:Lane A:0.5 mg RAJI Whole Cell Lysate4 uL anti-TMED2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMED2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)

IHC (Immunohistochemisry) (Immunochemical staining of human TMED2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry) (Immunochemical staining of human TMED2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-TMED2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Raji Whole Cell LysateLane D: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))

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TMED2, Polyclonal Antibody (Cat# AAA256083)

Full Name

Anti-TMED2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (AARSD1 was immunoprecipitated using:Lane A:0.5 mg 293 HepG2 Whole Cell Lysate4 uL anti-AARSD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AARSD1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size :48 kDa)

WB (Western Blot) (Anti-AARSD1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: 293 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:48 kDa)

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AARSD1, Polyclonal Antibody (Cat# AAA256112)

Full Name

Anti-AARSD1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (GKN1 was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-GKN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GKN1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size :24 kDa)

WB (Western Blot) (Anti-GKN1 rabbit polyclonal antibody at 1:500 dilutionLane A: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDa(We are unsure as to the identity of these extra bands.))

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Gastrokine 1, Polyclonal Antibody (Cat# AAA256235)

Full Name

Anti-Gastrokine 1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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FCM/FACS (Flow Cytometry) (AVIL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (AVIL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AVIL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of AVIL Antibody (N-term) in K562 cell line lysates (35ug/lane). AVIL (arrow) was detected using the purified Pab.)

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AVIL, Polyclonal Antibody (Cat# AAA289982)

Full Name

AVIL Antibody (N-term)

Gene Names

AVIL; p92; DOC6; ADVIL

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (GIPR Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (GIPR Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GIPR Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of GIPR antibody (N-term) in HL60 cell line lysates (35ug/lane). GIPR (arrow) was detected using the purified Pab.)

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GIPR, Polyclonal Antibody (Cat# AAA289899)

Full Name

GIPR Antibody (N-term)

Gene Names

GIPR; PGQTL2

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of Mitofusin using 293 whole cell lysates)

IHC (Immunohiostchemistry) (AAA327211 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, rat brain, using Mitofusin Antibody.)

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Mitofusin, Polyclonal Antibody (Cat# AAA327211)

Full Name

Mitofusin Antibody

Gene Names

MFN1; hfzo1; hfzo2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Porcine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human FUR Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Furin, Polyclonal Antibody (Cat# AAA142259)

Full Name

Polyclonal Antibody to Furin (FUR)

Gene Names

FURIN; FUR; PACE; SPC1; PCSK3

Reactivity

Human, Mouse, Rat, Pig

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Bone Morphogenetic Protein 5 (BMP5), Polyclonal Antibody (Cat# AAA133146)

Full Name

Polyclonal Antibody to Bone Morphogenetic Protein 5 (BMP5)

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Eye lysate; Primary Ab: 5ug/ml Rabbit Anti-Human RBP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Retinol Binding Protein 3, Interstitial (RBP3), Polyclonal Antibody (Cat# AAA131968)

Full Name

Polyclonal Antibody to Retinol Binding Protein 3, Interstitial (RBP3)

Gene Names

RBP3; IRBP; RBPI; RP66; D10S64; D10S65; D10S66

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $255/0.2 mL $410/0.5 mL $490/1 mL $1,320/5 mL $2,565/2x5 mL $3,740/3x5 mL $4,860/4x5 mL $5,940/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSTm2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Glutathione S Transferase Mu 2, Muscle (GSTm2), Polyclonal Antibody (Cat# AAA132378)

Full Name

Polyclonal Antibody to Glutathione S Transferase Mu 2, Muscle (GSTm2)

Gene Names

Gstm2; Gstb2; Gstb-2

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $265/0.2 mL $415/0.5 mL $500/1 mL $1,360/5 mL $2,645/2x5 mL $3,860/3x5 mL $5,010/4x5 mL $6,080/5x5 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using KLF7 Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using KLF7 Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of Humna colon cancer and Mouse kidney tissue, Mouse testis and Human fetal brain tissue using KLF7 Polyclonal Antibody at dilution of 1:483.3)

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KLF7, Polyclonal Antibody (Cat# AAA170667)

Full Name

KLF7 Polyclonal Antibody

Gene Names

KLF7; UKLF

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $245/0.06 mL $350/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,240/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using TMSB10 Polyclonal Antibody at dilution 1:25)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using TMSB10 Polyclonal Antibody at dilution 1:25)

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TMSB10, Polyclonal Antibody (Cat# AAA170364)

Full Name

TMSB10 Polyclonal Antibody

Gene Names

TMSB10; TB10; MIG12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SUGCT Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using SUGCT Polyclonal Antibody at dilution of 1:1000.)

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SUGCT, Polyclonal Antibody (Cat# AAA177620)

Full Name

SUGCT Polyclonal Antibody

Gene Names

SUGCT; ORF19; DERP13; C7orf10

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer using HSP90B1 Polyclonal Antibody at dilution of 1:30)

WB (Western Blot) (Western Blot analysis of Hela cell and Human liver cancer tissue, Jurkat and NIH/3T3 cell using HSP90B1 Polyclonal Antibody at dilution of 1:500)

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HSP90B1, Polyclonal Antibody (Cat# AAA168347)

Full Name

HSP90B1 Polyclonal Antibody

Gene Names

HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U20S cell using AZGP1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AZGP1 antibody.)

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AZGP1, Polyclonal Antibody (Cat# AAA167457)

Full Name

AZGP1 Antibody

Gene Names

AZGP1; ZAG; ZA2G

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human SS18L2 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human SS18L2 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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SS18L2, Polyclonal Antibody (Cat# AAA256433)

Full Name

Anti-SS18L2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IP (Immunoprecipitation) (PSPH was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-PSPH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSPH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :26 kDa)

WB (Western Blot) (Anti-PSPH rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:26 kDa)

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PSPH, Polyclonal Antibody (Cat# AAA256038)

Full Name

Anti-PSPH Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of CLDN7 in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CLDN7 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohistochemistry) (Immunochemical staining of human CLDN7 in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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Claudin 7, Polyclonal Antibody (Cat# AAA256236)

Full Name

Anti-Claudin 7 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Fusion, Polyclonal Antibody (Cat# AAA142457)

Full Name

Polyclonal Antibody to Fusion (FUS)

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant Tie1, Human.)

WB (Western Blot) (Western Blot: Sample: Human MCF7 Cells.)

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Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1, Polyclonal Antibody (Cat# AAA142269)

Full Name

Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Gene Names

TIE1; TIE; JTK14

Reactivity

Human, Mouse, Rat, Pig

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$185/0.1 mL $210/0.2 mL $315/0.5 mL $370/1 mL $945/5 mL $1,845/2x5 mL $2,690/3x5 mL $3,490/4x5 mL $4,270/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:MouseTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Cytokine Receptor Like Factor 1, Polyclonal Antibody (Cat# AAA141585)

Full Name

Polyclonal Antibody to Cytokine Receptor Like Factor 1 (CRLF1)

Gene Names

Crlf1; NR6; CLF-1; CRLM3; NR6.1; CRLM-3

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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FCM/FACS (Flow Cytometry) (HADHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of HADHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane). HADHB (arrow) was detected using the purified Pab.)

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HADHB, Polyclonal Antibody (Cat# AAA289182)

Full Name

HADHB Antibody (C-term)

Gene Names

HADHB; ECHB; MTPB; MSTP029; TP-BETA

Reactivity

Human (Predicted Reactivity: Bovine, Monkey)

Applications

Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (AAA321068 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on HeLa cell lysate using Dyskerin Antibody, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321068 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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Dyskerin, Polyclonal Antibody (Cat# AAA321068)

Full Name

Dyskerin Antibody

Gene Names

DKC1; DKC; CBF5; DKCX; NAP57; NOLA4; XAP101

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse esophagus using ORM1 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human kidney using ORM1 antibody at dilution of 1:200 (200x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ORM1 antibody.)

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ORM1, Polyclonal Antibody (Cat# AAA166546)

Full Name

ORM1 Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:MouseTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Interleukin 33 (IL33), Polyclonal Antibody (Cat# AAA132971)

Full Name

Polyclonal Antibody to Interleukin 33 (IL33)

Gene Names

Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Rat Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Rat Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat ITIH4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Inter Alpha-Globulin Inhibitor H4 (ITIH4), Polyclonal Antibody (Cat# AAA131699)

Full Name

Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $290/0.2 mL $480/0.5 mL $585/1 mL $1,575/5 mL $3,055/2x5 mL $4,470/3x5 mL $5,795/4x5 mL $7,085/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SLC2A2 Polyclonal Antibody at dilution 1:35)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using SLC2A2 Polyclonal Antibody at dilution 1:35)

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SLC2A2, Polyclonal Antibody (Cat# AAA167447)

Full Name

SLC2A2 Polyclonal Antibody

Gene Names

SLC2A2; GLUT2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of human placenta using CD31/PECAM1 Polyclonal Antibody at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat cells using CD31/PECAM1 Polyclonal Antibody at cells using CD31/PECAM1 Polyclonal Antibody at 1:1000 dilution.)

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CD31, Polyclonal Antibody (Cat# AAA178714)

Full Name

CD31 Polyclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using DNAJA1 Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer using DNAJA1 Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of HepG2, Raji, A431 and 231 cell using DNAJA1 Polyclonal Antibody at dilution of 1:800)

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DNAJA1, Polyclonal Antibody (Cat# AAA168588)

Full Name

DNAJA1 Polyclonal Antibody

Gene Names

DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using HPS4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HPS4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using HPS4 Polyclonal Antibody at dilution of 1:1000.)

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HPS4, Polyclonal Antibody (Cat# AAA177344)

Full Name

HPS4 Polyclonal Antibody

Gene Names

HPS4; LE

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human liver cancer using ALDH3A1 Polyclonal Antibody at dilution of 1:55)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human gastic cancer using ALDH3A1 Polyclonal Antibody at dilution of 1:55)

WB (Western Blot) (Western Blot analysis of A549 and Mouse eye tissue using ALDH3A1 Polyclonal Antibody at dilution of 1:750)

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ALDH3A1, Polyclonal Antibody (Cat# AAA169564)

Full Name

ALDH3A1 Polyclonal Antibody

Gene Names

ALDH3A1; ALDH3; ALDHIII

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of Hela cells using anti-SEC23A antibody (AAA125744).Overlay histogram showing Hela cells stained with AAA125744 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC23A Antibody (AAA125744, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 3. IF analysis of SEC23A using anti- SEC23A antibody (AAA125744).SEC23A was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SEC23A Antibody (AAA125744) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of SEC23A using anti-SEC23A antibody (AAA125744).SEC23A was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SEC23A Antibody (AAA125744) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SEC23A using anti-SEC23A antibody (AAA125744).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human PC-3 whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat stomach tissue lysatesLane 7: rat lung tissue lysatesLane 8: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SEC23A antigen affinity purified polyclonal antibody (Catalog # AAA125744) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SEC23A at approximately 86KD. The expected band size for SEC23A is at 86KD.)

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SEC23A, Polyclonal Antibody (Cat# AAA125744)

Full Name

Anti-SEC23A Antibody

Gene Names

SEC23A; CLSD

Reactivity

Human, Mouse, Rat

Applications

Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight488) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of ADAM29 in human testis tissue with ADAM29 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of ADAM29 in human testis tissue with ADAM29 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of ADAM29 in human bladder tissue lysate with ADAM29 antibody at (A) 1 and (B) 2 μg/ml.)

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ADAM29, Polyclonal Antibody (Cat# AAA40636)

Full Name

ADAM29 Antibody

Gene Names

ADAM29; CT73; svph1

Reactivity

Human

Applications

Western Blot

Purity

ADAM29 antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of TCTEX1D2 in K562 cells with TCTEX1D2 antibody at 20 μg/mL.)

WB (Western Blot) (Western blot analysis of TCTEX1D2 in K562 cell lysate with TCTEX1D2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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TCTEX1D2, Polyclonal Antibody (Cat# AAA40735)

Full Name

TCTEX1D2 Antibody

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

TCTEX1D2 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NudC (Phospho-Ser326) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells (Lane 2), using NudC (Phospho-Ser326) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from rat brain cells, using NudC (Phospho-Ser326) antibody. The lane on the right is treated with the synthesized peptide.)

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NUDC, Polyclonal Antibody (Cat# AAA243350)

Full Name

Phospho-NUDC (Ser326) Antibody

Gene Names

NUDC; HNUDC; MNUDC; NPD011

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western BlotPositive WB detected in: A549 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysateAll lanes: SNX5 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 14 kDaObserved band size: 47 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with AAA243489 at 1:213, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IHC (Immunohistochemistry) (IHC image of AAA243489 diluted at 1:640 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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SNX5, Polyclonal Antibody (Cat# AAA243489)

Full Name

SNX5 Antibody

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

>95%, Protein G Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of ZFP64 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZFP64 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human ZFP64 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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ZFP64, Polyclonal Antibody (Cat# AAA256423)

Full Name

Anti-ZFP64 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human GLB1 in human epididymis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human GLB1 in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

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GLB1, Polyclonal Antibody (Cat# AAA256286)

Full Name

Anti-GLB1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (PCCA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (PCCA Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PCCA antibody detected the PCCA protein (arrow).)

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PCCA, Polyclonal Antibody (Cat# AAA289550)

Full Name

PCCA Antibody (Center)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of HYOU1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysate from HepG2 cell line, using HYOU1 Antibody (Center). AAA289337 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

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HYOU1, Polyclonal Antibody (Cat# AAA289337)

Full Name

HYOU1 Antibody (Center)

Gene Names

HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (PVRL2/CD112 Antibody-Human Small Intestine, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (PVRL2/CD112 Antibody-Western blot analysis of extracts of various cell lines.)

ICC (Immunocytochemistry) (PVRL2/CD112 Antibody-Immunofluorescence analysis of HeLa cells.)

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PVRL2/CD112, Polyclonal Antibody (Cat# AAA162842)

Full Name

PVRL2/CD112 Rabbit anti-Human Polyclonal Antibody

Gene Names

NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

IHC (Immunohistochemistry) (Anti-TL1A antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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TNFSF15/TL1A/VEGI, Polyclonal Antibody (Cat# AAA162254)

Full Name

Anti-TNFSF15/TL1A/VEGI Antibody

Gene Names

TNFSF15; TL1; TL1A; VEGI; TNLG1B; VEGI192A

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using HLTF Polyclonal Antibody at dilution 1:40)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using HLTF Polyclonal Antibody at dilution 1:40)

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HLTF, Polyclonal Antibody (Cat# AAA166738)

Full Name

HLTF Polyclonal Antibody

Gene Names

HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using DDAH2 Polyclonal Antibody at dilution 1:20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DDAH2 Polyclonal Antibody at dilution 1:20)

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DDAH2, Polyclonal Antibody (Cat# AAA167052)

Full Name

DDAH2 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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WB (Western Blot) (Western blot analysis of CTLL-2 lysate using IL-2 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA325884 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA325884 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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IL2, Polyclonal Antibody (Cat# AAA325884)

Full Name

IL2 Antibody

Gene Names

IL2; IL-2; TCGF; lymphokine

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Cryptochrome 1, Polyclonal Antibody (Cat# AAA142472)

Full Name

Polyclonal Antibody to Cryptochrome 1 (CRY1)

Gene Names

CRY1; DSPD; PHLL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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WB (Western Blot) (Western blot analysis of extracts from JK cells, using HS1 (Ab-397) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of lysates from RajI,Daudi cell line (from left to right),using HS1 Antibody (Ab-397) (B8462). B8462 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

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HS1, Polyclonal Antibody (Cat# AAA288748)

Full Name

HS1 Antibody (Ab-397)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.