At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (Western Blot analysis of 1,mouse-liver 2,hela 3,mouse-brain cells using primary antibody diluted at 1:1000(4 degree C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25 degree C, 1 hour))
WB (Western Blot) (Western Blot analysis of hela cells using primary antibody diluted at 1:1000(4 degree C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25 degree C, 1 hour))
IHC (Immunohiostchemistry) (Cullin 4A/CUL4A Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot) (Cullin 4A/CUL4A Antibody-CUL4A/Cullin 4A antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IF (Immunofluorescence) (Immunofluorescence analysis of 293T cells using NAT2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast using NAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat heart using NAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat lung using NAT2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Daudi cells using anti-TROVE2/SS-A/RO60 antibody (AAA126305).Overlay histogram showing Daudi cells stained with AAA126305 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TROVE2/SS-A/RO60 Antibody (AAA126305, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of TROVE2/SS-A/RO60 using anti-TROVE2/SS-A/RO60 antibody (AAA126305).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: rat spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TROVE2/SS-A/RO60 antigen affinity purified polyclonal antibody (#AAA126305) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TROVE2/SS-A/RO60 at approximately 60 kDa. The expected band size for TROVE2/SS-A/RO60 is at 61 kDa.)
FCM/FACS (Flow Cytometry) (GTD2A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of GTD2A Antibody (C-term) in K562 cell line lysates (35ug/lane). GTD2A (arrow) was detected using the purified Pab.)
WB (Western Blot) (Lanes:Lane 1: 4ug DRM fraction from MDA-MB-231Primary Antibody Dilution:1:1000Secondary Antibody:Donkey anti-rabbit IgG-HRPSecondary Antibody Dilution:1:10000Gene Name:EGFRSubmitted by:Katarzyna Augoff, University of WroclawEGFR is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB231 cells)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TSEN15 Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using TSEN15 Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human stomach using SLC30A1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human mammary cancer using SLC30A1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IP (Immunoprecipitation) (RABEP1 was immunoprecipitated using:Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-RABEP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RABEP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :99 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of RABEP1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RABEP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot) (Anti-RABEP1 rabbit polyclonal antibody at 1:500 dilutionLane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:120 kDa)
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
DB (Dot Blot) (Dot blot analysis of anti-Phospho-IRS2-pY978 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
IHC (Immunohistochemistry) (PEA3/ETV4 Antibody-Anti-ETV4/PEA3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohistochemisry) (PEA3/ETV4 Antibody-Anti-ETV4/PEA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot) (PEA3/ETV4 Antibody-Fetal Small intestine Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot) (PEA3/ETV4 Antibody-ETV4/PEA3 antibody Western blot of Fetal small intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human skin using RAB6A Polyclonal Antibody at dilution of 1:25)
WB (Western Blot) (Western Blot analysis of 293T cell and Mouse brain tissue, A549, Hela, A172 and HT-29 cell using RAB6A Polyclonal Antibody at dilution of 1:650)
IHC (Immunohiostchemistry) (Immunochemical staining of human OXSM in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human OXSM in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IF (Immunofluorescence) (Immunofluorescent analysis of SMAD4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of SMAD4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot) (Western blot analysis of SMAD4 expression in HepG2 (A), HeLa (B), NIH3T3 (C), COS7 (D) whole cell lysates.)
SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 50 ug, Lane 1-3: A549 cells, K562 cells, hela cells, Primary antibody: AAA239195(NAT10 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA239195(NAT10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA239195(NAT10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200))
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by antigen-affinity chromatography.
Pricing
WB (Western Blot) (CCR9 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CCR9 antibody detected the CCR9 protein (arrow).)
WB (Western Blot) (APG4C Antibody (Y48) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the APG4C antibody detected the APG4C protein (arrow).)
IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot) (The anti-APG4C Pab is used in Western blot to detect APG4C in mouse liver tissue lysate)
WB (Western Blot) (Western blot analysis of anti-hAPG4C-Y48 Pab in 293 cell line lysates transiently transfected with the ATG4C gene (2ug/lane). hAPG4C-Y48(arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot) (Western blot analysis of MBD3 (arrow) using rabbit polyclonal MBD3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).)
WB (Western Blot) (ZEB1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ?g) probed with ZEB1 antibody (1:1,000).)
WB (Western Blot) (ZEB1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ZEB1 antibody was used to immunoprecipitate ZEB1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ZEB1 antibody at a dilution of 1:1,000.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MCCC1 Polyclonal Antibody at dilution of 1:45(×200))
WB (Western Blot) (Western blot analysis of 293T HepG2 A172 cell Mouse heart tissue Jurkat cell lysates using MCCC1 Polyclonal Antibody at dilution of 1:300)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SLC25A38 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of 293T cells using SLC25A38 Polyclonal Antibody at dilution of 1:3000.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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