At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human brain using PGK1 Polyclonal Antibody at dilution of 1:40)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using PGK1 Polyclonal Antibody at dilution of 1:40)
WB (Western Blot) (Western Blot analysis of A549 and hela cell, Mouse liver and Human liver tissue, hepG2 cell using PGK1 Polyclonal Antibody at dilution of 1:850)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using SPTA1 Polyclonal Antibody at dilution of 1:80(×200))
WB (Western Blot) (Western blot analysis of Mouse heart tissue lysate using SPTA1 Polyclonal Antibody at dilution of 1:550)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using GluR6 antibody.)
WB (Western Blot) (Western blot analysis of lysates from K562 cell line,using mGluR6 Antibody(C0210). C0210 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug.)
IF (Immunofluorescence) (AAA326335 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohiostchemistry) (AAA326335 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CYP11A1 antibody.)
IF (Immunofluorescence) (AAA327097 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using MCM5 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA327097 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (Immunofluorescence staining of CALCOCO2 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CALCOCO2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry) (Immunochemical staining of human CALCOCO2 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human CALCOCO2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 293 cells using EMILIN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of EMILIN1 Antibody (N-term) in K562 and 293 cell line lysates (35ug/lane). EMILIN1 (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of CEM cells using HMHA1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lymph reacted with HMHA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of HMHA1 Antibody (Center) in A2058 cell line lysates (35ug/lane). HMHA1 (arrow) was detected using the purified Pab.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using RSU1 Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using RSU1 Polyclonal Antibody at dilution of 1:30(×200))
WB (Western Blot) (Western blot analysis of Rat heart tissue Mouse heart tissue Rat brain tissue Mouse brain tissue HEPG2 cell Jurkat and Lovo cell lysates using RSU1 Polyclonal Antibody at dilution of 1:400)
IHC (Immunohiostchemistry) (PTGR2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTGR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (PTGR2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTGR2 antibody detected the PTGR2 protein (arrow).)
WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using OR5P2 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using OR5P2 Antibody. The picture on the right is blocked with the synthesized peptide.)
SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Human liver tissue, K562 cells, human fetal brain tissue, Raji cells, Primary antibody: AAA239207(NDUFS5 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA239207(NDUFS5 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA239207(NDUFS5 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43(Phospho-Ser367) Antibody(left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using Connexin 43(phospho-Ser368) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Application Data (Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B . Image courtesy of Dr. R. Kapito, Stanford University)
Application Data (HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) followed by Sheep anti Rabbit IgG:HRP)
IHC (Immunohiostchemistry) (GRIN3B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRIN3B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (GRIN3B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GRIN3B antibody detected the GRIN3B protein (arrow).)
IP (Immunoprecipitation) (PSMC5 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-PSMC5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMC5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :46 kDa)
IHC (Immunohiostchemistry) (Immunochemical staining of human PSMC5 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-PSMC5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot) (Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. HOMER antibody diluted at 1:20000.)
WB (Western Blot) (Homer antibody detects HOMER1 protein by Western blot analysis. A. 30 ug IMR32 whole cell lysate/extract. 10 % SDS-PAGE. Homer antibody dilution:1:1000)
WB (Western Blot) (Homer antibody detects HOMER1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 10 % SDS-PAGE. Homer antibody dilution:1:500)
ICC (Immunocytochemistry) (Immunofluorescence of methanol-fixed HeLa using Homer antibody at 1:500 dilution.)
IHC (Immunohistochemistry) (Anti-HOMER1 / Homer 1 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)
IHC (Immunohistochemistry) (Anti-IGFBP7/TAF antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)
FCM/FACS (Flow Cytometry) (RPS4Y1 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (RPS4Y1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPS4Y1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (RPS4Y1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPS4Y1 antibody detected the RPS4Y1 protein (arrow).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of GOT1L1 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry) (GOT1L1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GOT1L1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (GOT1L1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GOT1L1 antibody detected the GOT1L1 protein (arrow).)
IHC (Immunohiostchemistry) (MLPH Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLPH Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (MLPH Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MLPH antibody detected the MLPH protein (arrow).)
IHC (Immunohiostchemistry) (MRAP2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRAP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (MRAP2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MRAP2 antibody detected the MRAP2 protein (arrow).)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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