Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Immunohistochemical analysis of GLP-1 Receptor staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of GLP-1 Receptor expression in mouse brain (A), rat brain (B) whole cell lysates.)

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GLP-1 Receptor, Polyclonal Antibody (Cat# AAA27814)

Full Name

Anti-GLP-1 Receptor Antibody

Gene Names

GLP1R; GLP-1; GLP-1R; GLP-1-R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was purified by affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Bovine Colon Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine CerebeluM Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Bovine Spinal cord Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine Kidney Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine CerebuM Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Bovine Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: Bovine Small intestine Tissue; Primary Ab: 20ug/ml Rabbit Anti-Bovine HB Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Hemoglobin (HB), Polyclonal Antibody (Cat# AAA21251)

Full Name

Polyclonal Antibody to Hemoglobin (HB)

Gene Names

HBA; HBA2; alpha globin

Reactivity

Human, Bovine

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$230/0.1 mL $285/0.2 mL $425/0.5 mL $505/1 mL $1,310/5 mL $2,525/2x5 mL $3,685/3x5 mL $4,770/4x5 mL $5,785/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: SMN2 knockout 293T cell lysate; Predicted MW: 32,30,29,27kd Observed MW: 37kd Primary Ab: 1ug/ml Rabbit Anti-Human SMN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Serum; Lane2: Human Hela Cells; Lane3: Human HepG2 Cells; Lane4: Human 293T Cells; Lane5: Mouse Testis Tissue.)

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Survival Of Motor Neuron 2, Polyclonal Antibody (Cat# AAA21031)

Full Name

Survival Of Motor Neuron 2, Centromeric (SMN2) Polyclonal Antibody

Gene Names

SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testis tissue using AAA26942 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA26942 at dilution of 1:100)

WB (Western Blot) (Western blotAll lanes: ZBTB32 antibody at 4ug/mlLane 1: Mouse brain tissueLane 2: Jurkat whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

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ZBTB32, Polyclonal Antibody (Cat# AAA26942)

Full Name

ZBTB32 Antibody

Gene Names

ZBTB32; Rog; FAXF; FAZF; TZFP; ZNF538

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using AAA26961 at dilution 1:100)

WB (Western Blot) (Western blotAll lanes: SLC1A5 antibody at 9.89 ug/mlLane 1: Mouse lung tissueLane 2: Jurkat whole cell lysateLane 3: 293T whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 57,37,34 kDaObserved band size: 57,37 kDa)

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SLC1A5, Polyclonal Antibody (Cat# AAA26961)

Full Name

SLC1A5 Antibody

Gene Names

SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA26991 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

WB (Western Blot) (Positive WB detected in:HepG2 whole cell lysate, A549 whole cell lysate, Mouse stomach tissue, Mouse lung tissueAll lanes: PARD6A antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

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PARD6A, Polyclonal Antibody (Cat# AAA26991)

Full Name

PARD6A Antibody

Gene Names

PARD6A; PAR6; PAR6C; TAX40; PAR-6A; TIP-40; PAR6alpha

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western blot analysis of extracts from K562 cells, using Collagen XI alph1 antibody.)

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Collagen XI alpha1, Polyclonal Antibody (Cat# AAA31109)

Full Name

Collagen XI alpha1 Antibody

Gene Names

COL11A1; STL2; COLL6; CO11A1

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: MALD1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 kDaObserved band size: 34 kDa)

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MALD1, Polyclonal Antibody (Cat# AAA26975)

Full Name

MALD1 Antibody

Reactivity

Apple

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunofluorescence) (Figure 17. IF analysis of HADHA using anti-HADHA antibody (AAA19797).HADHA was detected in an immunocytochemical section of MG63 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 15. IHC analysis of HADHA using anti-HADHA antibody (AAA19797).HADHA was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADHA Antibody (AAA19797) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat kidney tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse kidney tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human HELA whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa.Lane 1: human 293T whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHA antigen affinity purified polyclonal antibody (#AAA19797) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HADHA at approximately 79 kDa. The expected band size for HADHA is at 83 kDa.)

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HADHA, Polyclonal Antibody (Cat# AAA19797)

Full Name

Anti-HADHA Antibody Picoband

Gene Names

HADHA; GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

WB (Western Blot)

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ABCA3, Polyclonal Antibody (Cat# AAA17974)

Full Name

ABCA3 Antibody

Gene Names

ABCA3; ABC3; ABC-C; SMDP3; LBM180; EST111653

Reactivity

Human
Predicted: Mouse, Rat

Applications

Western Blot

Pricing

$465/0.1 mg $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

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IF (Immunofluorescence) (Immunofluorescence analysis of A431 cells using RAB11A/RAB11B Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using RAB11A/RAB11B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using RAB11A/RAB11B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using RAB11A/RAB11B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain using RAB11A/RAB11B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using RAB11A/RAB11B Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using RAB11A/RAB11B Polyclonal Antibody at 1:1000 dilution.)

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RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA22272)

Full Name

RAB11A/RAB11B Polyclonal Antibody

Gene Names

RAB11A; YL8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$330/0.06 mL $445/0.12 mL $675/0.2 mL $1,290/2x0.2 mL $1,875/3x0.2 mL $2,420/4x0.2 mL $2,905/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta tissue using AAA26966 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in:Hela whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysateAll lanes: HPSE antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62,55,54,43 kDaObserved band size: 62 kDa)

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HPSE, Polyclonal Antibody (Cat# AAA26966)

Full Name

HPSE Antibody

Gene Names

HPSE; HPA; HPA1; HPR1; HSE1; HPSE1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung tissue using AAA26920 at dilution of 1:100)

IF (Immunofluorescence) (Immunofluorescent analysis of PC3 cells using AAA26920 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate,Rat kidney tissueAll lanes: TROVE2 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,24,59,60 KDaObserved band size: 61 KDa)

IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA26920 at a dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver tissue using AAA26920 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, PC3 whole cell lysate, Mouse heart tissue,Mouse spleen tissue,Mouse lung tissueAll lanes: TROVE2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 61,24,59,6 kDaobserved band size: 61 kDa)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using AAA26920 at dilution of 1:20)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kiney using AAA26920 at dilution of 1:20)

WB (Western Blot) (Western blotAll lanes: TROVE2SS-A/Ro ribonucleoprotein antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 61,24,59,60 kDaObserved band size: 61 kDa)

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60 kDa SS-A/Ro ribonucleoprotein, Polyclonal Antibody (Cat# AAA26920)

Full Name

Rabbit anti-human 60 kDa SS-A/Ro ribonucleoprotein polyclonal Antibody

Gene Names

TROVE2; RO60; SSA2; RORNP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney tissue using AAA26928 at dilution of 1:100)

WB (Western Blot) (Western blotAll lanes: PBLD antibody at 6ug/mlLane 1: Mouse liver tissueLane 2: Mouse kidney tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 32 kDaObserved band size: 32 kDa)

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PBLD, Polyclonal Antibody (Cat# AAA26928)

Full Name

PBLD Antibody

Gene Names

PBLD; MAWBP; MAWDBP; HEL-S-306

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G Purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using Pan Methyl Lysine Polyclonal Antibody at dilution of 1:2000.)

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Pan Methyl Lysine, Polyclonal Antibody (Cat# AAA22056)

Full Name

Pan Methyl Lysine Polyclonal Antibody

Reactivity

All

Applications

Western Blot

Purity

Affinity purification

Pricing

$205/0.02 mL $255/0.06 mL $370/0.12 mL $545/0.2 mL $1,030/2x0.2 mL $1,485/3x0.2 mL $1,920/4x0.2 mL $2,165/5x0.2 mL

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IHC (Immunohistochemistry) (Immunofluorescent analysis of HeLa cells using AAA26917 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon tissue using AAA26917 at dilution of 1:100)

WB (Western Blot) (Western blotAll lanes: PNPLA2 antibody at 10ug/mlLane 1: Mouse liver tissueLane 2: NIH/3T3 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 56,20 kDaObserved band size: 56 kDa)

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PNPLA2, Polyclonal Antibody (Cat# AAA26917)

Full Name

PNPLA2 Antibody

Gene Names

PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney tissue using AAA26931 at dilution 1:100)

WB (Western Blot) (Western blotAll lanes: METRNL antibody at 2ug/mlLane 1: Mouse small intestine tissueLane 2: Mouse kidney tissueLane 3: Rat gonadal tissueLane 4: Mouse brain tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 35, 26 kDaObserved band size: 35 kDa)

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Meteorin-like protein, Polyclonal Antibody (Cat# AAA26931)

Full Name

Rabbit anti-human Meteorin-like protein polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26939 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

WB (Western Blot) (All lanes: ACKR1 antibody at 2ug/mlLane 1:mouse kidney tissueLane 2mouse spleen tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 36kDaObserved band size: 36kDa)

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ACKR1, Polyclonal Antibody (Cat# AAA26939)

Full Name

ACKR1 Antibody

Gene Names

ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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Application Data (Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human CALML5 Polyclonal Antibody (AAA21028, red histogram) or Isotype control antibody (Catalog , green histogram), followed by 1ug/ml FITC conjugated Anti-rabbit IgG Secondary Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Breast cancer TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Sample: Recombinant CALML5, Human.)

WB (Western Blot) (Sample: Human A431 cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Human CALML5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Calmodulin Like Protein 5, Polyclonal Antibody (Cat# AAA21028)

Full Name

Calmodulin Like Protein 5 (CALML5) Polyclonal Antibody

Gene Names

CALML5; CLSP

Reactivity

Human

Applications

Westen Blot, Immunohistochemistry

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

Application Data

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Tryptophan Hydroxylase, Polyclonal Antibody (Cat# AAA14234)

Full Name

Anti-Tryptophan Hydroxylase

Applications

Dot Blot, Immunohistochemistry, Western Blot

Purity

Affinity Purified Sheep polyclonal antibody.

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,085/5x0.1 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: ATP1b3 knockout A431 cell lysate; Predicted MW: 32kd Observed MW: 55kd Primary Ab: 5ug/ml Rabbit Anti-Human ATP1b3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human A-431 Cells; Lane3: Rat Skin Tissue.)

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ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), Polyclonal Antibody (Cat# AAA20304)

Full Name

Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)

Gene Names

ATP1B3; CD298; ATPB-3

Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$235/0.1 mL $290/0.2 mL $430/0.5 mL $520/1 mL $1,340/5 mL $2,580/2x5 mL $3,755/3x5 mL $4,865/4x5 mL $5,925/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: XPo1 knockout Hela cell lysate; Predicted MW: 123kd Observed MW: 110kd Primary Ab: 1ug/ml Rabbit Anti-Human XPO1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human Lung Tissue; Lane4: Porcine Intestine Tissue.)

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Exportin 1, Polyclonal Antibody (Cat# AAA21017)

Full Name

Exportin 1 (XPO1) Polyclonal Antibody

Gene Names

XPO1; emb; CRM1; exp1

Reactivity

Human, Pig

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: human CerebuM Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Thyroid cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Endometrial cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Breast cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Lung cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human Ovarian cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-ulti-species CT Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Calcitonin (CT), Polyclonal Antibody (Cat# AAA21262)

Full Name

Polyclonal Antibody to Calcitonin (CT)

Reactivity

General

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Affinity Chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,435/5 mL $2,770/2x5 mL $4,035/3x5 mL $5,230/4x5 mL $6,400/5x5 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26979 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta tissue using AAA26979 at dilution of 1:100)

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SLC30A8, Polyclonal Antibody (Cat# AAA26979)

Full Name

SLC30A8 Antibody

Gene Names

SLC30A8; ZNT8; ZnT-8

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testicle tissue using AAA26982 at dilution of 1:300)

IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26982 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

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ERVW-1, Polyclonal Antibody (Cat# AAA26982)

Full Name

ERVW-1 Antibody

Gene Names

ERVW-1; ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent analysis of mcf-7 cells using AAA26958 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human heart tissue using AAA26958 at dilution 1:100)

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DRAM2, Polyclonal Antibody (Cat# AAA26958)

Full Name

DRAM2 Antibody

Gene Names

DRAM2; CORD21; PRO180; TMEM77; WWFQ154

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$265/0.05 mL $370/0.1 mL $690/2x0.1 mL $995/3x0.1 mL $1,285/4x0.1 mL $1,515/5x0.1 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: SMC3 knockout Jurkat cell lysate; Predicted MW: 142kd Observed MW: 142kd Primary Ab: 3ug/ml Rabbit Anti-Human SMC3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)

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Structural Maintenance Of Chromosomes Protein 3 (SMC3), Polyclonal Antibody (Cat# AAA20309)

Full Name

Polyclonal Antibody to Structural Maintenance Of Chromosomes Protein 3 (SMC3)

Gene Names

SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1

Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

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KO (Knockout Validation) (Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: CASP8 knockout Hela cell lysate;;Predicted MW: 26-32,46,54-62kDa ;Observed MW: 60kDa;Primary Ab: 3ug/ml Rabbit Anti-Human CASP8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human HepG2 Cells; Lane3: Human Hela Cells.)

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Caspase 8, Polyclonal Antibody (Cat# AAA21012)

Full Name

Caspase 8 (CASP8) Polyclonal Antibody

Gene Names

CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8

Reactivity

Human, Pig

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $265/0.2 mL $395/0.5 mL $465/1 mL $1,205/5 mL $2,325/2x5 mL $3,385/3x5 mL $4,385/4x5 mL $5,345/5x5 mL

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IHC (Immunohistochemistry) (Immunofluorescent analysis of Hela cells using AAA26970 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testicular tissue using AAA26970 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer tissue using AAA26970 at dilution of 1:100)

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TLL1, Polyclonal Antibody (Cat# AAA26970)

Full Name

TLL1 Antibody

Gene Names

TLL1; TLL; ASD6

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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WB (Western Blot) (Western BlotPositive Western Blot (WB) detected in Recombinant proteinAll lanes: UNC80 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 59 KDaobserved band size: 59 KDa)

IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26981 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L))

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UNC80, Polyclonal Antibody (Cat# AAA26981)

Full Name

UNC80 Antibody

Gene Names

UNC80; UNC-80; C2orf21

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of JUNB using anti-JUNB antibody (AAA19249).JUNB was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-JUNB Antibody (AAA19249) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of JUNB using anti-JUNB antibody (AAA19249).JUNB was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-JUNB Antibody (AAA19249) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of JUNB using anti-JUNB antibody (AAA19249).JUNB was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-JUNB Antibody (AAA19249) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of JUNB using anti-JUNB antibody (AAA19249).JUNB was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-JUNB Antibody (AAA19249) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of JUNB using anti-JUNB antibody (AAA19249).JUNB was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-JUNB Antibody (AAA19249) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of JUNB using anti-JUNB antibody (AAA19249).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-JUNB antigen affinity purified polyclonal antibody (Catalog # AAA19249) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for JUNB at approximately 43-45KD. The expected band size for JUNB is at 36KD.)

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JUNB, Polyclonal Antibody (Cat# AAA19249)

Full Name

Anti-JUNB Antibody

Gene Names

JUNB; AP-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.)

WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.)

WB (Western Blot) (WB Suggested Anti-HES1 Antibody Titration: 1.25ug/mlPositive Control: Raji cell lysateHES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells)

WB (Western Blot) (HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.)

IP (Immunoprecipitation) (K562)

IHC (Immunohistochemistry) (Rabbit Anti-HES1 AntibodyParaffin Embedded Tissue: Human cardiac cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IF (Immunofluorescence) (transfected Ls174T cell lysate at 50ug/ml.)

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HES1, Polyclonal Antibody (Cat# AAA23407)

Full Name

HES1 antibody - N-terminal region

Gene Names

HES1; HHL; HRY; HES-1; bHLHb39

Reactivity

Tested Species Reactivity: Human Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish

Applications

Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

$475/0.1 mL $905/2x0.1 mL $1,310/3x0.1 mL $1,690/4x0.1 mL $1,890/5x0.1 mL

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WB (Western Blot) (Host: RabbitTarget Name: NADSYN1Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NADSYN1Sample Tissue: Human 786-0 Whole Cell Antibody Dilution: 1ug/ml)

WB (Western Blot) (Host: Rabbit Target Name: NADSYN1 Sample Tissue: Human HT1080 Whole Cell Antibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NADSYN1Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 3ug/ml)

WB (Western Blot) (Host: RabbitTarget: NADSYN1Positive control (+): MCF7 Cell Lysate (N10)Negative control (-): HepG2 Cell Lysate (HG)Antibody concentration: 3ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NADSYN1Sample Type: PANC1 Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

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NADSYN1, Polyclonal Antibody (Cat# AAA23597)

Full Name

NADSYN1 Antibody - C-terminal region

Reactivity

Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Yeast

Applications

Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with (AAA26935) at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IHC (Immunohistochemistry) (IHC image of (AAA26935) diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (IHC image of (AAA26935) diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in:Zebrafish 40ug,20ug,10ugAll lanes: Sox2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)

WB (Western Blot) (Western BlotPositive WB detected in: mouse brainAll lanes: SOX2 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 kDaObserved band size: 34 kDa)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using AAA26935 at dilution of 1:100)

WB (Western Blot) (All lanes: Transcription factor SOX-2 antibody at 6ug/ml+293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 34kDaObserved band size: 34kDa)

WB (Western Blot) (All lanes: Transcription factor SOX-2 antibody at 2ug/ml+mouse kidney tissueSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 34kDaObserved band size: 34kDa)

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Transcription factor SOX-2, Polyclonal Antibody (Cat# AAA26935)

Full Name

Rabbit anti-human Transcription factor SOX-2 polyclonal Antibody

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-KCNQ2 antibody. Overlay histogram showing 293T cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of KCNQ2 using anti-KCNQ2 antibody.KCNQ2 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KCNQ2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of KCNQ2 using anti-KCNQ2 antibody.KCNQ2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KCNQ2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of KCNQ2 using anti-KCNQ2 antibody. KCNQ2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KCNQ2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (KCNQ2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KCNQ2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of KCNQ2 using anti-KCNQ2 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNQ2 at approximately 97 kDa. The expected band size for KCNQ2 is at 97 kDa.)

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KCNQ2, Polyclonal Antibody (Cat# AAA19238)

Full Name

Anti-KCNQ2 Antibody

Gene Names

KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of JK cells using anti-HDHD5 antibody (AAA20013).Overlay histogram showing JK cells stained with AAA20013 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDHD5 Antibody (AAA20013, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of HDHD5 using anti-HDHD5 antibody (AAA20013).HDHD5 was detected in a paraffin-embedded section of rat brain(hippocampus) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HDHD5 Antibody (AAA20013) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD5 antigen affinity purified polyclonal antibody (#AAA20013) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDHD5 at approximately 50 kDa. The expected band size for HDHD5 is at 46 kDa.)

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HDHD5, Polyclonal Antibody (Cat# AAA20013)

Full Name

Anti-HDHD5 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of KLF15 using anti-KLF15 antibody (AAA19496).KLF15 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KLF15 Antibody (AAA19496) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of KLF15 using anti-KLF15 antibody (AAA19496).KLF15 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KLF15 Antibody (AAA19496) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of KLF15 using anti-KLF15 antibody (AAA19496).KLF15 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KLF15 Antibody (AAA19496) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of KLF15 using anti-KLF15 antibody (AAA19496).KLF15 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KLF15 Antibody (AAA19496) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of KLF15 using anti-KLF15 antibody (AAA19496).KLF15 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-KLF15 Antibody (AAA19496) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of KLF15 using anti-KLF15 antibody (AAA19496).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF15 antigen affinity purified polyclonal antibody (#AAA19496) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KLF15 at approximately 44 kDa. The expected band size for KLF15 is at 44 kDa.)

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KLF15, Polyclonal Antibody (Cat# AAA19496)

Full Name

Anti-KLF15 Antibody Picoband

Gene Names

KLF15; KKLF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) ( Host: RabbitTarget Name: FMODSample Tissue: Human MCF7 Whole CellAntibody Dilution: 3ug/ml)

WB (Western Blot) (Host: RabbitTarget: FMODPositive control (+): 293T (2T)Negative control (-): Human kidney (KI)Antibody concentration: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-FMOD Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysate)

WB (Western Blot) (Sample Type: Equine Cartilage ExplantsPrimary Dilution: 1:500Secondary: Bio-Rad 170-5046 (Dilution: 1:100,000))

WB (Western Blot) (Host: RabbitTarget Name: FMODSample Tissue: Human U937 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FMODSample Tissue: Human RPMI 8226 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FMODSample Tissue: Human HelaAntibody Dilution: 1.0ug/ml)

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FMOD, Polyclonal Antibody (Cat# AAA23557)

Full Name

FMOD antibody - N-terminal region

Gene Names

FMOD; FM; SLRR2E

Reactivity

Tested Species Reactivity: Human, Horse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Applications

Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver tissue using AAA26971 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid tissue using AAA26971 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in: Mouse kidney tissueAll lanes: CCL3 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 11 KDaObserved band size: 11 KDa)

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CCL3, Polyclonal Antibody (Cat# AAA26971)

Full Name

CCL3 Antibody

Gene Names

CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

Application Data

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Lubricin/PRG4, Polyclonal Antibody (Cat# AAA17958)

Full Name

Lubricin/PRG4 Antibody

Gene Names

PRG4; MSF; SZP; CACP; HAPO; JCAP

Reactivity

Human, Mouse, Rat
Not yet tested in other species.

Applications

Peptide ELISA, Western Blot

Pricing

$465/0.1 mg $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PGAM2 knockout Hela cell lysate; Predicted MW: 29kd Observed MW: 29kd Primary Ab: 1ug/ml Rabbit Anti-Human PGAM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein)

WB (Western Blot) (Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PGAM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PGAM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PGAM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Phosphoglycerate Mutase 2, Polyclonal Antibody (Cat# AAA21013)

Full Name

Phosphoglycerate Mutase 2, Muscle (PGAM2) Polyclonal Antibody

Gene Names

PGAM2; GSD10; PGAMM; PGAM-M

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PGM1 knockout Hela cell lysate; Predicted MW: 64,61,40kd Observed MW: 64kd Primary Ab: 1ug/ml Rabbit Anti-Human PGM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Porcine Liver Tissue; Lane4: Porcine Heart Tissue.)

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Phosphoglucomutase 1, Polyclonal Antibody (Cat# AAA21030)

Full Name

Phosphoglucomutase 1 (PGM1) Polyclonal Antibody

Gene Names

PGM1; CDG1T; GSD14

Reactivity

Human, Pig

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-METTL3 antibody (AAA19248).Overlay histogram showing THP-1 cells stained with AAA19248 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (AAA19248, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of METTL3 using anti-METTL3 antibody (AAA19248).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human HELA whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody (Catalog # AAA19248) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for METTL3 at approximately 75KD. The expected band size for METTL3 is at 75KD.)

IF (Immunofluorescence) (Figure 5. IF analysis of METTL3 using anti- METTL3 antibody (AAA19248).METTL3 was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- METTL3 Antibody (AAA19248) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

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METTL3, Polyclonal Antibody (Cat# AAA19248)

Full Name

Anti-METTL3 Antibody

Gene Names

METTL3; M6A; IME4; Spo8; MT-A70

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3) using a 12 % SDS PAGE gel and IL22 Receptor alpha 2 antibody at a dilution of 1:200)

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IL22 Receptor alpha 2, Polyclonal Antibody (Cat# AAA17935)

Full Name

IL22 Receptor alpha 2 antibody

Gene Names

IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; ZCYTOR16; IL-22R-alpha-2

Applications

Western Blot, Immunohistochemistry

Purity

Antigen-affinity chromatography

Pricing

$1,400/0.1 mL $2,700/2x0.1 mL $3,935/3x0.1 mL $5,095/4x0.1 mL $5,975/5x0.1 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: KLK11 knockout K562 cell lysate; Predicted MW: 30kd Observed MW: 40kd Primary Ab: 2ug/ml Rabbit Anti-Human KLK11 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Mouse Thymus Tissue; Lane2: Mouse Skin Tissue; Lane3: Human K562 Cells.)

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Kallikrein 11 (KLK11), Polyclonal Antibody (Cat# AAA20306)

Full Name

Polyclonal Antibody to Kallikrein 11 (KLK11)

Gene Names

KLK11; TLSP; PRSS20

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$235/0.1 mL $290/0.2 mL $435/0.5 mL $520/1 mL $1,345/5 mL $2,590/2x5 mL $3,770/3x5 mL $4,885/4x5 mL $5,965/5x5 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat stomach using Slc39a11 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using Slc39a11 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Slc39a11 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using Slc39a11 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using Slc39a11 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using Slc39a11 Polyclonal Antibody at 1:1000 dilution.)

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Slc39a11, Polyclonal Antibody (Cat# AAA22273)

Full Name

Slc39a11 Polyclonal Antibody

Gene Names

Slc39a11; 1810074D23Rik

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$325/0.06 mL $435/0.12 mL $655/0.2 mL $1,245/2x0.2 mL $1,810/3x0.2 mL $2,340/4x0.2 mL $2,855/5x0.2 mL

IHC (Immunohistochemistry-Paraffin) (Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

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CD20, Polyclonal Antibody (Cat# AAA21271)

Full Name

Anti-CD20 Antibody IHC-plus

Gene Names

MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic acid and ammonium sulfate precipitation

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

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Application Data (Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)

WB (Western Blot) (Host: Rabbit Target Name: SPAM1 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 0.2ug/ml.)

WB (Western Blot) (Host: Rabbit Target Name: SPAM1 Sample Tissue: Human HT1080 Whole Cell Antibody Dilution: 1ug/ml.)

WB (Western Blot) (Host: Rabbit Target Name: SPAM1 Sample Tissue: Human Jurkat Whole Cell Antibody Dilution: 3ug/mlb.)

WB (Western Blot) (Host: Rabbit Target Name: SPAM1 Sample Tissue: Human A549 Whole Cell Antibody Dilution: 3ug/ml.)

WB (Western Blot) (WB Suggested Anti-SPAM1 AntibodyTitration: 1.0 ug/mlPositive Control: Jurkat Whole Cell)

WB (Western Blot) (Host: RabbitTarget Name: SPAM1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SPAM1Sample Type: 293TAntibody Dilution: 1.0ug/ml)

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SPAM1, Polyclonal Antibody (Cat# AAA23593)

Full Name

SPAM1 Antibody - C-terminal region

Gene Names

SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: EEF1a1 knockout Jurkat cell lysate; Predicted MW: 48,50kd Observed MW: 50kd Primary Ab: 1ug/ml Rabbit Anti-Human EEF1a1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Mouse Heart Tissue; Lane4: Mouse Brain Tissue.)

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Eukaryotic Translation Elongation Factor 1 Alpha 1, Polyclonal Antibody (Cat# AAA21009)

Full Name

Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) Polyclonal Antibody

Gene Names

EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

WB (Western Blot) (western blot All lanes:Interferon gamma antibody at 2ug/ml+Recombinant Guinea Pig Interferon gamma protein 10ug Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19kDa Observed band size: 19kDa)

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Interferon gamma, Polyclonal Antibody (Cat# AAA18716)

Full Name

IFNG Antibody, Biotin conjugated

Reactivity

Guinea Pig

Applications

ELISA

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.