Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

IHC (Immunohistochemistry) (IHC: Human ovary carcinoma stained with Anti-Legumain (AAA14072) 1:200 dilution. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

WB (Western Blot) (Western Blot: The cell lysate derived from HT-29 (lane 1), Hela (Lane 2) and mouse kidney (lane 3) were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rb anti Legumain antibody (AAA14072) at 1:500. An immunoreactive band around ~50 kDa was observed.)

Compare (5 max)

Legumain, Polyclonal Antibody (Cat# AAA14072)

Full Name

Rabbit anti Legumain Polyclonal Antibody

Gene Names

LGMN; AEP; LGMN1; PRSC1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Epitope Affinity Purification

Pricing

$390/0.1 mg $730/2x0.1 mg $1,055/3x0.1 mg $1,365/4x0.1 mg $1,445/5x0.1 mg

ELISA (Competitive inhibition ELISA;Coated protein:5-HIAA conjugated to BSA (Catalog: Series Diluted 5-HIAA;Primary Ab: 2ug/ml Mouse Anti DHEA Antibody;Second Ab: 2ug/mL HRP-Linked Caprine Anti-mouse IgG Polyclonal Antibody (Catalog: IC50=12ng/ml)

Compare (5 max)

5-Hydroxyindoleacetic Acid, Polyclonal Antibody (Cat# AAA21023)

Full Name

5-Hydroxyindoleacetic Acid (5-HIAA) Polyclonal Antibody

Reactivity

General

Applications

Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$265/0.1 mL $320/0.2 mL $525/0.5 mL $630/1 mL $1,670/5 mL $3,230/2x5 mL $4,705/3x5 mL $6,095/4x5 mL $7,440/5x5 mL

WB (Western Blot) (Positive WB detected in Recombinant proteinAll lanes: kgp antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 56 KDaObserved band size: 56 KDa)

Compare (5 max)

kgp, Polyclonal Antibody (Cat# AAA27000)

Full Name

kgp Antibody

Reactivity

Porphyromonas gingivalis

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry) (LAG3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LAG3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of LAG3 Antibody (Center) in K562 cell line lysates (35ug/lane). LAG3 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Anti-LAG3 Antibody (Center)at 1:2000 dilution (please inquire) whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LAG3 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysates Lane 2: human spleen lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

WB (Western Blot) (Anti-LAG3 Antibody (Center)at 1:2000 dilution + K562 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LAG3 Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: human liver lysates Lane 3: human ovary lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-LAG3 Antibody (Center) at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-LAG3 Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LAG3 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

FCM (Flow Cytometry) (Overlay histogram showing Jurkat cells stained with AAA28766 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28766, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

IHC (Immunohistochemistry) (AAA28766 staining LAG3 in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Compare (5 max)

LAG3, Polyclonal Antibody (Cat# AAA28766)

Full Name

LAG3 Antibody (Center)

Gene Names

LAG3; CD223

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (TMPRSS2 Antibody (N-term) (RB18784) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TMPRSS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

FCM (Flow Cytometry) (TMPRSS2 Antibody (N-term) (AAA28791) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies wereused for the analysis.)

WB (Western Blot) (Western blot analysis of TMPRSS2 Antibody (N-term) (AAA28791) in 293, NCI-H460 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)

Compare (5 max)

TMPRSS2, Polyclonal Antibody (Cat# AAA28791)

Full Name

TMPRSS2 Antibody (N-term)

Gene Names

TMPRSS2; PP9284; PRSS10

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

IHC (Immunohistchemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

WB (Western Blot) (Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti- THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)

FCM (Flow Cytometry) (Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)

WB (Western Blot) (Dilution: Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Pituitary (Mouse) Lysate at 40 ug Lane 3: Adrenal gland (Mouse) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Pituitary (Rat) Lysate at 40 ug Lane 7: Adrenal gland (Rat) Lysate at 40 ug Lane 8: Muscle (Rat) Lysate at 40 ug Lane 9: K562 (Human) Cell Lysate at 30 ug Lane 10: Siha (Human) Cell Lysate at 30 ug Lane 11: A431 (Human) Cell Lysate at 30 ug Lane 12: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)

FCM (Flow Cytometry) (Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)

FCM (Flow Cytometry) (Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)

Compare (5 max)

THRB1, Polyclonal Antibody (Cat# AAA28811)

Full Name

THRB1 Polyclonal Antibody

Reactivity

Rat, Cow

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded Human kidney using SLC5A2 Rabbit pAb)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded Rat kidney using SLC5A2 Rabbit pAb)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of SLC5A2 in paraffin-embedded rat kidney using SLC5A2 Rabbit pAb)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of SLC5A2 in paraffin-embedded mouse kidney using SLC5A2 Rabbit pAb)

WB (Western Blot) (Western blot analysis of various lysates, using SLC5A2 Rabbit pAb)

IF (Immunofluorescence) (Immunofluorescence analysis of human kidney using SLC5A2 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat kidney using SLC5A2 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using SLC5A2 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SLC5A2 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SLC5A2 antibody.)

Compare (5 max)

SLC5A2, Polyclonal Antibody (Cat# AAA30730)

Full Name

SLC5A2 Rabbit Polyclonal Antibody

Gene Names

SLC5A2; SGLT2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

Application Data (Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021)

Application Data (Zhao, TingTing, et al. "Tangshen formula modulates gut Microbiota and reduces gut-derived toxins in diabetic nephropathy rats." Biomedicine & Pharmacotherapy 129 (2020): 110325.)

Application Data (Yi, Sheng, et al. "Peripheral nerve injury induces dynamic changes of tight junction components." Frontiers in physiology9 (2018): 1519.)

Application Data (Chang, Ying, et al. "Lentivirus-mediated knockdown of astrocyte elevated gene-1 inhibits growth and induces apoptosis through MAPK pathways in human retinoblastoma cells." PloS one 11.2 (2016): e0148763.)

Application Data (Wang, Xiao, et al. "Knockdown of Phospholipase C? (PLC?) Inhibits Cell Proliferation via Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN)/AKT Signaling Pathway in Human Prostate Cancer." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 254.)

Application Data (Wang, Chunhua, et al. "Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-?B Pathway In Vitro." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 7654.)

WB (Western Blot) (Western blot analysis of 293T (1), Rat brain (2), NIH 3T3 (3), Sheep Muscle (4), Rabbit testis (5), diluted at 1:20000. Secondary antibody was diluted at 1:20000)

Compare (5 max)

GAPDH, Polyclonal Antibody (Cat# AAA30771)

Full Name

GAPDH Rabbit Polyclonal Antibody

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat, Rb, Ch, Mk, sheep, X

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Pricing

$335/0.05 mL $415/0.1 mL $785/2x0.1 mL $1,130/3x0.1 mL $1,455/4x0.1 mL $1,550/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)

Application Data (Gel: 12%SDS-PAGE Lysates (from left to right): NIH/3T3, K562 and 293T cellAmount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000Exposure time: 20 seconds)

Compare (5 max)

CD63, Polyclonal Antibody (Cat# AAA29720)

Full Name

CD63 Antibody

Gene Names

CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Antigen affinity purification.

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ryanodine Receptor 2 (phospho-Ser2808) expression in U251 whole cell lysates.)

Compare (5 max)

Ryanodine Receptor 2 (phospho-Ser2808), Polyclonal Antibody (Cat# AAA29761)

Full Name

Ryanodine Receptor 2 (phospho-Ser2808) Antibody

Gene Names

RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Immunogen affinity chromatography

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (AAA30959 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30959 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30959 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30959 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30959 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA30959 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Chk2 phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Chk2, Polyclonal Antibody (Cat# AAA30959)

Full Name

Phospho-Chk2 (Ser516) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-EGFR (Ser1071) expression in various lysates)

WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in serum treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30964 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA30964 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30964 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30964 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30964 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA30964)

Full Name

Phospho-EGFR (Ser1071) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-c-Jun (Thr93) expression in various lysates)

IHC (Immunohistchemistry) (AAA30983 at 1/100 staining rat intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30983 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30983 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA30983)

Full Name

Phospho-c-Jun (Thr93) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IS (Immunostaining) (Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)

IS (Immunostaining) (Immunostaining of 25µm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)

WB (Western Blot) ((0.5µg/ml) staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)

IF (Immunofluorescence) (AAA13656 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence) (AAA13656 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

ChIP (Chromatin Immunoprecipitation) (ChIP of 2ug AAA13656 with 1ug DHT-treated HEC50 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring FKBP5 enrichment.)

IHC (Immunohistochemistry) (AAA13656 (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Compare (5 max)

Androgen Receptor, Polyclonal Antibody (Cat# AAA13656)

Full Name

Goat anti-Androgen Receptor Antibody

Gene Names

AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Applications

Peptide ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$485/0.1 mg $925/2x0.1 mg $1,340/3x0.1 mg $1,725/4x0.1 mg $1,905/5x0.1 mg

Application Data

Compare (5 max)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA13774)

Full Name

Anti-Spike Protein (SARS-CoV-2) Polyclonal Antibody

Applications

Western Blot

Purity

Protein G immunoaffinity chromatography

Pricing

$435/0.1 mg $830/2x0.1 mg $1,190/3x0.1 mg $1,530/4x0.1 mg $1,665/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) (IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry) (IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)

IHC (Immunohistchemistry) (IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)

IF (Immunofluorescence) (Immunofluorescence of primary RPE cells with ZO1 Ab at 1/100 dilution.)

IF (Immunofluorescence) (Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence) (Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence) (Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence) (Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

WB (Western Blot) (Anti-ZO1 Ab at 1/2,500 dilution; 50ug of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.)

Immunoperoxidase (Immunoperoxidase of polyclonal antibody to ZO-1 (1/200) on paraformaldehyde-fixed paraffin-embedded mouse kidney.)

Reactivity Chart

Compare (5 max)

ZO-1, Polyclonal Antibody (Cat# AAA13861)

Full Name

ZO-1 Polyclonal Antibody

Gene Names

Tjp1; ZO1

Reactivity

Reacts against Human, Rat, Mouse, Canine and Monkey proteins.

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Epitope-affinity purified from goat antiserum

Pricing

$390/0.6 mg $730/2x0.6 mg $1,055/3x0.6 mg $1,365/4x0.6 mg $1,500/5x0.6 mg

Application Data

Compare (5 max)

MMP-9, Polyclonal Antibody (Cat# AAA14085)

Full Name

Rabbit anti MMP-9 Polyclonal antibody

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Reactivity

Human, Rat, Mouse, Chicken, Dog

Applications

Western Blot, Immunohistochemistry

Purity

The Rabbit IgG is purified by Epitope Affinity Purification.

Pricing

$390/0.1 mg $730/2x0.1 mg $1,055/3x0.1 mg $1,365/4x0.1 mg $1,445/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Collagen 2 alpha 1 expression in MCF7 (A) whole cell lysates.)

Compare (5 max)

Collagen 2 alpha 1, Polyclonal Antibody (Cat# AAA27787)

Full Name

Anti-Collagen 2 alpha 1 Antibody

Gene Names

COL2A1; AOM; ANFH; SEDC; STL1; COL11A3

Reactivity

Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis of 600ug extracts of Mouse thymus cells using 3ug STAT5B antibody. Western blot was performed from the immunoprecipitate using STAT5B antibody at a dilution of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using STAT5B Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using STAT5B Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using STAT5B Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat intestine using [KO Validated] STAT5B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] STAT5B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse intestin using [KO Validated] STAT5B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] STAT5B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] STAT5B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts from wild type (WT) and STAT5B knockout (KO) HeLa cells, using STAT5B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using STAT5B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Compare (5 max)

STAT5B, Polyclonal Antibody (Cat# AAA28416)

Full Name

[KO Validated] STAT5B Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence - anti-CTSD Ab at 1/100 dilution in RAW264.7 cells; cells were fixed with PFA and permeabilized with 0.05% saponin)

IF (Immunofluorescence) (Immunofluorescence- anti CTSD at 1/100 dilution in NHI/3T3; cells were fixed with methanol and permeabilized with 0.1% saponin)

Application Data (Immunogold lableing using melanocytes and anti-CTSDS Ab)

Application Data (Immunoperoxidase of polyclonal antibody to CTSDS (1:200) on paraformaldehyde-fixed paraffin embedded mouse lung)

Application Data

Reactivity Chart

Compare (5 max)

Cathepsin D, Polyclonal Antibody (Cat# AAA13859)

Full Name

Cathepsin D Polyclonal Antibody

Gene Names

CTSD; CPSD; CLN10; HEL-S-130P

Reactivity

Reacts against human, rat, mouse, canine and monkey proteins.

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry

Pricing

$390/0.6 mg $510/1.5 mg $975/2x1.5 mg $1,415/3x1.5 mg $1,825/4x1.5 mg $2,070/5x1.5 mg

Application Data

Compare (5 max)

Shaker, Polyclonal Antibody (Cat# AAA14091)

Full Name

Rabbit anti Shaker

Reactivity

Drosophila

Applications

Western Blot, Immunohistochemistry

Purity

The rabbit IgG is purified by Epitope Affinity Purification.

Pricing

$390/0.1 mg $730/2x0.1 mg $1,055/3x0.1 mg $1,365/4x0.1 mg $1,445/5x0.1 mg

ELISA

Compare (5 max)

NA (A/California/06/2009)(H1N1), Polyclonal Antibody (Cat# AAA13728)

Full Name

Anti-NA (A/California/06/2009)(H1N1), rabbit IgG

Applications

Western Blot

Purity

Immunoaffinity chromatography

Pricing

$480/0.1 mg $915/2x0.1 mg $1,325/3x0.1 mg $1,705/4x0.1 mg $1,895/5x0.1 mg

< >

<< Previous Next >>

IF (Immunofluorescence) (IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Anti- Band 3 Picoband antibody, AAA11648,IHC(F)IHC(F): Human Placenta Tissue)

IHC (Immunohistochemistry) (Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Mouse Kidney Tissue)

IHC (Immunohistochemistry) (Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Human Placenta Tissue)

Application Data (Anti- Band 3 Picoband antibody, AAA11648, IHC(P)IHC(P): Rat Kidney Tissue)

WB (Western Blot) (Anti- Band 3 Picoband antibody, Western blottingAll lanes: Anti Band 3 at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Rat Spleen Tissue Lysate at 50ugLane 5: Human Placenta Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)

Compare (5 max)

Band 3, Polyclonal Antibody (Cat# AAA11648)

Full Name

Anti-Band 3 Antibody

Gene Names

SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A

Reactivity

Human, Mouse , Rat.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

WB (Western Blot) (Western blot of rat synaptic membrane (SPM) showing specific immunolabeling of the ~36 kDa stargazin protein.)

Compare (5 max)

Stargazin, Polyclonal Antibody (Cat# AAA14233)

Full Name

Anti-Stargazin

Gene Names

Cacng2; stg; wag; waggler; AW060990; stargazer; stargazin; B230105C07Rik; B930041E13Rik

Reactivity

Tested: Rat
Expected: Mouse

Applications

Western Blot

Purity

Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column to which the C-terminal peptide used as immunogen was coupled.

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,085/5x0.1 mL

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PI3K P85 a/y/beta antibody (left) or the same antibody preincubated with with blocking peptide (right))

Compare (5 max)

PI3K, Polyclonal Antibody (Cat# AAA17933)

Full Name

PI3K antibody

Applications

Immunohistochemistry

Purity

PI3K antibody was purified by immunoaffinity chromatography

Pricing

$655/0.05 mg $1,245/2x0.05 mg $1,810/3x0.05 mg $2,340/4x0.05 mg $2,640/5x0.05 mg

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA26962 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human testis tissue using AAA26962 at dilution 1:100)

WB (Western Blot) (Western BlotPositive WB detected in:HEK293 whole cell lysate,K562 whole cell lysate,Mouse brain tissueAll lanes: BTNL2 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51,44,31,28,20,29 kDaObserved band size: 51 kDa)

Compare (5 max)

BTNL2, Polyclonal Antibody (Cat# AAA26962)

Full Name

BTNL2 Antibody

Gene Names

BTNL2; SS2; BTN7; BTL-II; HSBLMHC1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: ompK antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 44 KDaobserved band size: 44 KDa)

Compare (5 max)

ompK, Polyclonal Antibody (Cat# AAA26976)

Full Name

ompK Antibody

Reactivity

Vibrio parahaemolyticus serotype O3: K6

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°½ over night)

Compare (5 max)

TCEAL7, Polyclonal Antibody (Cat# AAA29731)

Full Name

TCEAL7 Antibody

Gene Names

TCEAL7; WEX5; MPMGp800C04260Q003

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Immunogen affinity purified

Pricing

$305/0.05 mL $365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human muscle using Collagen XII alpha 1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)

Compare (5 max)

COL12A1, Polyclonal Antibody (Cat# AAA27944)

Full Name

COL12A1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purified

Pricing

$415/0.1 mL $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cell using MMP16 antibody (AAA28228) at dilution of 1:100. Blue: DAPI for nuclear staining)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (AAA28228) at dilution of 1:100 (40x lens))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (AAA28228) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (AAA28228) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (AAA28228) at dilution of 1:100 (40xlens))

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MMP16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Compare (5 max)

MMP16, Polyclonal Antibody (Cat# AAA28228)

Full Name

MMP16 Polyclonal Antibody

Gene Names

MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (RGS1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of RGS1 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). RGS1 (arrow) was detected using the purified Pab.)

Compare (5 max)

RGS1, Polyclonal Antibody (Cat# AAA28739)

Full Name

RGS1 Antibody (N-term)

Gene Names

RGS1; 1R20; BL34; IER1; IR20; HEL-S-87

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (ENOB Antibody (Center) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow).)

Compare (5 max)

ENOB, Polyclonal Antibody (Cat# AAA28761)

Full Name

ENOB Antibody (Center)

Gene Names

ENO3; MSE; GSD13

Reactivity

Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (AAA28762), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-ACE2 C-term Pab (AAA28762)in K562 cell line lysates (35ug/lane). ACE2(arrow) was detected using the purified Pab.)

WB (Western Blot) (The anti-ACE2 C-term Pab (AAA28762) is used in Western blot to detect ACE2 in 293 cell lysate.)

Compare (5 max)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

Full Name

ACE2 (SARS Receptor) Antibody (C-term)

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

WB (Western Blot) (All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

WB (Western Blot) (All lanes :DCAMKL1 Antibody (C-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes :DCAMKL1 Antibody (C-term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IF (Immunofluorescence) ((C): The cell positive for DCAMKL-1, stained red (indicated by the arrow), appears at the base of the crypt. (D): Intestinal section stained for Musashi-1 (green). (E): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow). The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1. (F): Costaining of DCMAKL-1 (red, indicated by the arrow) with nuclear Hoechst 33342 (blue) staining. (G): Costaining of Musashi-1 (green) with nuclear Hoechst 33342 (blue) staining. (H): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow), costained with nuclear Hoechst 33342 (blue) staining. The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1 (yellow).)

IHC (Immunohistochemistry) (Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).)

WB (Western Blot) (The DCAMKL1 Antibody (C-term) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).)

Compare (5 max)

DCAMKL1, Polyclonal Antibody (Cat# AAA28779)

Full Name

DCAMKL1 Antibody (C-term)

Gene Names

DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with LGR5/GPR49 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28712 LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292).)

WB (Western Blot) (Western blot analysis of lysates from Hela, HepG2, SH-SY5Y cell line (from left to right), using LGR5/GPR49 Antibody (loop2). AAA28712 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse skeletal muscle lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: mouse skeletal muscle lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: rat skeletal muscle lysatesLane 3: Hela whole cell lysatesLane 4: HepG2 whole cell lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLane 3: R.skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

LGR5/GPR49, Polyclonal Antibody (Cat# AAA28712)

Full Name

LGR5/GPR49 Antibody (loop2)

Gene Names

LGR5; FEX; HG38; GPR49; GPR67; GRP49

Reactivity

Human, mouse, rat

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$270/0.05 mL $500/0.2 mL $955/2x0.2 mL $1,385/3x0.2 mL $1,780/4x0.2 mL $1,980/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (AAA30946 at 1/100 staining rat cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30946 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30946 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30946 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Chk1 phosphorylation expression in Na3VO4 treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30946 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA30946 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Chk1 phosphorylation expression in Na3VO4 treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Chk1, Polyclonal Antibody (Cat# AAA30946)

Full Name

Phospho-Chk1 (Ser286) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-c-Jun (Ser63) expression in various lysates)

IHC (Immunohistchemistry) (AAA30980 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30980 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30980 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

c-Jun, Polyclonal Antibody (Cat# AAA30980)

Full Name

Phospho-c-Jun (Ser63) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Tau (Ser235) expression in various lysates)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30997 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30997 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30997 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Tau phosphorylation expression in COLO205 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Tau, Polyclonal Antibody (Cat# AAA30997)

Full Name

Phospho-Tau (Ser235) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of KRAS Antibody (C-term)(AAA28748) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (All lanes : Anti-KRAS Antibody (C-term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: HT-29 whole cell lysateLane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysateLane 5: Ramos whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-KRAS Antibody (C-term) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: C2C12 whole cell lysateLane 4: C6 whole cell lysateLane 5: HT-29 whole cell lysateLane 6: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDa)

WB (Western Blot) (Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

KRAS, Polyclonal Antibody (Cat# AAA28748)

Full Name

KRAS Antibody (C-term)

Gene Names

KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B

Reactivity

Human, Mouse, Rat (Predicted: Rat)

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

WB (Western Blot) (Anti-Zebrafish hey2 Antibody (C-term)at 1:2000 dilution + ZF4 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

hey2, Polyclonal Antibody (Cat# AAA28798)

Full Name

Zebrafish hey2 Antibody (C-term)

Gene Names

hey2; grl; zgc:136746

Reactivity

Zebrafish

Applications

Western Blot

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (SLC5A12 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC5A12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Anti-SLC5A12 Antibody (C-term) at 1:2000 dilution + Y79 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

SLC5A12, Polyclonal Antibody (Cat# AAA28799)

Full Name

SLC5A12 Antibody (C-term)

Gene Names

SLC5A12; SMCT2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

WB (Western Blot) (DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)

WB (Western Blot) (Anti-DRD2 Antibody (C-term)at 1:2000 dilution + human brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-DRD2 Antibody (C-term)at 1:2000 dilution + HT-1080 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-DRD2 Antibody (C-term)at 1:1000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-DRD2 Antibody (C-term)at 1:2000 dilution + human brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-DRD2 Antibody (C-term) at 1:2000 dilutionLane 1: human brain lysatesLane 2: K562 whole cell lysatesLane 3: human cerebellum lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

DRD2, Polyclonal Antibody (Cat# AAA28691)

Full Name

DRD2 Antibody (C-term)

Gene Names

DRD2; D2R; D2DR

Reactivity

Human, mouse

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of MUC1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of MUC1 expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)

Compare (5 max)

MUC1, Polyclonal Antibody (Cat# AAA27799)

Full Name

Anti-MUC1 Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$255/0.03 mL $285/0.05 mL $345/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,655/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/30.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/30.)

Compare (5 max)

MEIS1, Polyclonal Antibody (Cat# AAA29656)

Full Name

MEIS1 Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen affinity purification.

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-Tyrosine Hydroxylase (Ser19) expression in various lysates)

IF (Immunofluorescence) (AAA30988 staining Hela cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IF (Immunofluorescence) (AAA30988 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA30988 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30988 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Tyrosine Hydroxylase phosphorylation expression in Insulin treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Compare (5 max)

Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA30988)

Full Name

Phospho-Tyrosine Hydroxylase (Ser19) Antibody

Gene Names

TH; TYH; DYT14; DYT5b

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Phospho-ERK1/2 (Thr202/Tyr204) Antibody for IHC in human testis)

WB (Western Blot) (Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30930 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

IHC (Immunohistchemistry) (AAA30930 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30930 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30930 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30930 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30930 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Compare (5 max)

ERK1/2, Polyclonal Antibody (Cat# AAA30930)

Full Name

Phospho-ERK1/2 (Thr202/Tyr204) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.