Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Anti-5HT1A Receptor antibody, AAA11520, IHC(P)IHC(P): Rat Brain Tissue)

WB (Western Blot) (Anti-5HT1A Receptor antibody, AAA11520, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Human U87 Cell Lysate)

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5HT1A Receptor, Polyclonal Antibody (Cat# AAA11520)

Full Name

Anti-5HT1A Receptor antibody

Gene Names

HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1

Reactivity

Reacts with: Human, Rat
Predicted to work with: Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 9 Immunohistochemistry Validation of IRF7 in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IHC (Immunohistochemistry) (Figure 8 Immunohistochemistry Validation of IRF7 in Human Pancreas Tissue Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IHC (Immunohistochemistry) (Figure 7 Immunohistochemistry Validation of IRF7 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of IRF7 in Mouse Raw264.7 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with 8991 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot) (Figure 5 Western Blot Validation in Rat TissuesLoading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 4 Western Blot Validation in Mouse TissuesLoading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Western Blot Validation in Human TissuesLoading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: IRF7, 8991, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 1 KO Validation in A549 Cell Lysate Loading: 10 μg of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: IRF7 8991 (0.5 μg/mL) and beta-actin 3779 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)

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IRF7, Polyclonal Antibody (Cat# AAA11032)

Full Name

IRF7 Antibody

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

IRF7 Antibody is Protein A purified.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

WB (Western Blot) (Western Blot Data: 1: 293 cell extract control 2: 293 cell expressing E2 (HCV))

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E2 (HCV), Polyclonal Antibody (Cat# AAA13709)

Full Name

Anti-E2 (HCV), rabbit IgG

Applications

Western Blot

Purity

Immunoaffinity chromatography

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,575/5x0.1 mg

IHC (Immunohistochemistry)

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p16, Polyclonal Antibody (Cat# AAA13850)

Full Name

p16 (C20) Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry

Pricing

$360/0.2 mg $670/2x0.2 mg $965/3x0.2 mg $1,245/4x0.2 mg $1,295/5x0.2 mg

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WB (Western Blot) (Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

Immunogold (Immunogold labeling of epithelium cells, in vivo injected with YFP expressing vector;)

IF (Immunofluorescence) (Immunofluorescence – anti-YFP Ab in 293HEK cells transfected with YFP at 1/50 dilution; cells were fixed with 4% of PFA;)

Reactivity Chart

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YFP, Polyclonal Antibody (Cat# AAA13878)

Full Name

YFP Polyclonal Antibody

Reactivity

Transfected cells proteins

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

This antibody is epitope-affinity purified from goat antiserum.

Pricing

$390/0.3 mg $730/2x0.3 mg $1,055/3x0.3 mg $1,365/4x0.3 mg $1,500/5x0.3 mg

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Reactivity Chart (RC)

Reactivity Chart

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TagRFP, Polyclonal Antibody (Cat# AAA13884)

Full Name

anti-TagRFP

Reactivity

Transfected cells proteins

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy

Purity

This antibody is epitope-affinity purified from goat antiserum

Pricing

$390/0.3 mg $730/2x0.3 mg $1,055/3x0.3 mg $1,365/4x0.3 mg $1,500/5x0.3 mg

WB (Western Blot) (Western BlotPositive WB detected in: Rosseta bacteria lysate at 80ug, 40ug, 20ug, 10ugAll lanes: rpsB antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 27 kDaobserved band size: 27 kDa)

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30S ribosomal protein S2, Polyclonal Antibody (Cat# AAA15915)

Full Name

Rabbit anti-Escherichia coli 30S ribosomal protein S2 polyclonal Antibody

Gene Names

rpsB; ECK0168; JW0164

Reactivity

Escherichia coli

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

WB (Western Blot) (Western Blot analysis of KB, Mouse lung using Pan myristoylation Polyclonal Antibody at dilution of 1:1000.)

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Pan myristoylation, Polyclonal Antibody (Cat# AAA22115)

Full Name

Pan myristoylation Polyclonal Antibody

Reactivity

All

Applications

Western Blot

Purity

Affinity purification

Pricing

$205/0.02 mL $255/0.06 mL $370/0.12 mL $545/0.2 mL $1,030/2x0.2 mL $1,485/3x0.2 mL $1,920/4x0.2 mL $2,165/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of parafffin-embedded rat spleen using Lysozyme Polyclonal Antibody at dilution of 1:400.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of parafffin-embedded mouse spleen using Lysozyme Polyclonal Antibody at dilution of 1:400.)

WB (Western Blot) (Western Blot analysis of various samples using Lysozyme Polyclonal Antibodyat dilution of 1:1000.)

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Lysozyme, Polyclonal Antibody (Cat# AAA22122)

Full Name

Lysozyme Polyclonal Antibody

Gene Names

Lyz2; Lys; Lzm; Lzp; Lysm; Lyzs; Lyzf2; Lzm-s1; AI326280

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$290/0.06 mL $380/0.12 mL $535/0.2 mL $1,015/2x0.2 mL $1,470/3x0.2 mL $1,900/4x0.2 mL $2,280/5x0.2 mL

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WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.)

WB (Western Blot) (Host: RabbitTarget: RIPK3Positive control (+): THP-1 (N30)Negative control (-): A549 (N03)Antibody concentration: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-RIPK3 Antibody Titration: 1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate)

WB (Western Blot) (WB Suggested Anti-RIPK3 antibody Titration: 1 ug/mLSample Type: Human Jurkat)

WB (Western Blot) (WB Suggested Anti-RIPK3 antibody Titration: 1 ug/mLSample Type: Human HepG2)

WB (Western Blot) (Host: RabbitTarget Name: RIPK3Sample Type: Lane 1: Transient overexpression lysate of RIPK3Lane 2: Non-overexpressed vector control lysateAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (RIPK3 antibody - N-terminal regionFormalin Fixed Paraffin Embedded Tissue: Human Pancreas TissueObserved Staining: Cytoplasmic in endocrine part (islets) of the pancreas. No staining observed in exocrine cellsPrimary Antibody Concentration: 3 -12 ug/mlSecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure: 0.2 seconds)

IHC (Immunohistochemistry) (Human Liver)

IHC (Immunohistochemistry) (Human Heart)

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RIPK3, Polyclonal Antibody (Cat# AAA23385)

Full Name

RIPK3 antibody - N-terminal region

Gene Names

RIPK3; RIP3

Reactivity

Tested Species Reactivty: Human
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

$475/0.1 mL $905/2x0.1 mL $1,310/3x0.1 mL $1,690/4x0.1 mL $1,890/5x0.1 mL

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Application Data (Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)

FCM (Flow Cytometry) (CF150 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody (Center). AAA28789 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody (Center). AAA28789 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody (Center). AAA28789 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). AAA28789 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

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CF150, Polyclonal Antibody (Cat# AAA28789)

Full Name

CF150 Antibody (Center)

Gene Names

MB21D1; cGAS; h-cGAS; C6orf150

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistochemistry) (AAA30939 at 1/100 staining rat appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30939 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining human duodenum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30939 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30939 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Chk1 phosphorylation expression in UV treated HT29 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30939 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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Chk1, Polyclonal Antibody (Cat# AAA30939)

Full Name

Phospho-Chk1 (Ser280) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

WB (Western Blot) (Western blot analysis Rpt6 (Phospho-Ser120) using 293 whole cell lysates)

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Rpt6, Polyclonal Antibody (Cat# AAA29757)

Full Name

Rpt6 (Phospho-Ser120) Antibody

Gene Names

PSMC5; S8; p45; SUG1; SUG-1; TBP10; TRIP1; p45/SUG

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$305/0.05 mL $365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KO Validated] COX5A Rabbit pAb (AAA28619) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] COX5A Rabbit pAb (AAA28619) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KO Validated] COX5A Rabbit pAb (AAA28619) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] COX5A Rabbit pAb (AAA28619) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] COX5A Rabbit pAb (AAA28619) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and COX5A knockdown (KD) HeLa(KD) cells, using [KO Validated] COX5A Rabbit pAb (AAA28619) at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit pAb (AAA28619) at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

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COX5A, Polyclonal Antibody (Cat# AAA28619)

Full Name

[KO Validated] COX5A Rabbit pAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IHC (Immunohistochemistry) (Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)

WB (Western Blot) (Anti-5HT2A Receptor antibody, AAA11510, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue LysateLane 5: U87 Cell LysateLane 6: SMMC Cell LysateLane 7: HT1080 Cell LysateLane 8: COLO320 Cell Lysate)

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5HT2A Receptor, Polyclonal Antibody (Cat# AAA11510)

Full Name

Anti-5HT2A Receptor antibody

Gene Names

HTR2A; HTR2; 5-HT2A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

Application Data (Human C-Reactive Protein detected using Goat anti Human C-Reactive Protein (AAA12241))

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C-REACTIVE PROTEIN, Polyclonal Antibody (Cat# AAA12241)

Full Name

GOAT ANTI HUMAN C-REACTIVE PROTEIN

Gene Names

CRP; PTX1

Reactivity

Human

Applications

Immunodiffusion

Purity

Purified IgG by affinity chromatography.

Pricing

$440/1 mL $840/2x1 mL $1,205/3x1 mL $1,555/4x1 mL $1,680/5x1 mL

ELISA (Pig VEGF-A ELISA Recombinant Pig VEGF-A detected using Goat anti Pig VEGF-A as the capture reagent and Goat anti Pig VEGF-A:Biotin as the detection reagent followed by Streptavidin:HRP)

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VEGF-A, Polyclonal Antibody (Cat# AAA12254)

Full Name

Goat anti Pig VEGF-A: Biotin

Gene Names

VEGFA; VEGF

Applications

ELISA

Pricing

$825/0.05 mg $1,575/2x0.05 mg $2,285/3x0.05 mg $2,960/4x0.05 mg $3,385/5x0.05 mg

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IHC (Immunohistochemistry) (IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry) (IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

IHC (Immunohistchemistry) (IHC of mouse retina using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry) (IHC of human skin using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry) (IHC of human heart using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry) (IHC of human lymph node using anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

Application Data

Reactivity Chart

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CANX, Polyclonal Antibody (Cat# AAA13872)

Full Name

Calnexin Polyclonal Antibody

Gene Names

canx; zgc:63524; wu:fe06b12

Reactivity

Human, Rat, Mouse, Monkey, Canine proteins

Applications

Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry

Pricing

$390/0.4 mg $510/1 mg $975/2x1 mg $1,415/3x1 mg $1,825/4x1 mg $2,070/5x1 mg

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-METTL3 antibody (AAA19247).Overlay histogram showing HL-60 cells stained with AAA19247 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (AAA19247, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of METTL3 using anti- METTL3 antibody (AAA19247).METTL3 was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- METTL3 Antibody (AAA19247) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4 IHC analysis of METTL3 using anti-METTL3 antibody (AAA19247).METTL3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19247) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3 IHC analysis of METTL3 using anti-METTL3 antibody (AAA19247).METTL3 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19247) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19247).METTL3 was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19247) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of METTL3 using anti-METTL3 antibody (AAA19247).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human HELA whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: rat testis tissue lysatesLane 8: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody (Catalog # AAA192471) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for METTL3 at approximately 64KD. The expected band size for METTL3 is at 64KD.)

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METTL3, Polyclonal Antibody (Cat# AAA19247)

Full Name

Anti-METTL3 Antibody

Gene Names

METTL3; M6A; IME4; Spo8; MT-A70

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

WB (Western Blot) (Positive WB detected in: pat 293T Transfected lysate, 293T non-Transfected lysateAll lanes: pat antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 kDaObserved band size: 49 kDa)

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pat, Polyclonal Antibody (Cat# AAA26978)

Full Name

pat Antibody

Reactivity

Streptomyces viridochromogenes

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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WB (Western Blot) (Western blot of BAFF/BLyS in mouse and human cell lysates. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human).)

IHC (Immunohistochemistry) (Anti-BAFF antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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BAFF, Polyclonal Antibody (Cat# AAA12314)

Full Name

Rabbit Polyclonal (IgG) to Human BAFF

Gene Names

TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Protein G Purified

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

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IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

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Calponin, Polyclonal Antibody (Cat# AAA14404)

Full Name

Calponin Antibody

Gene Names

CNN1; SMCC; Sm-Calp; HEL-S-14

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Pricing

$520/0.1 mg $985/2x0.1 mg $1,425/3x0.1 mg $1,845/4x0.1 mg $2,030/5x0.1 mg

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WB (Western Blot) (CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody (AAA28787), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-CHAK1 Antibody (AAA28787) (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.)

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TRPM7 (CHAK1), Polyclonal Antibody (Cat# AAA28787)

Full Name

TRPM7 (CHAK1) Antibody (C-term)

Gene Names

TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK

Reactivity

Human, Mouse
Predicted: Rat

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (human cervix cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistchemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (human cervix cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry) (Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

FCM (Flow Cytometry) (Blank control: Lovo Cells(blue). Primary Antibody: Rabbit Anti-Giantin/AF488 Conjugated antibody (bs-13356R-AF488), Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.)

FCM (Flow Cytometry) (Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µg in 100 µL1X PBS containing 0.5% BSA(green).)

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Giantin, Polyclonal Antibody (Cat# AAA28815)

Full Name

Giantin Polyclonal Antibody

Gene Names

GOLGB1; GCP; GCP372; GOLIM1

Reactivity

Rat, Dog

Applications

Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (SERPINH1 Antibody (Center) (RB18952) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINH1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of SERPINH1 antibody (Center) (Cat#AAA28730) in HepG2 cell line lysates (35ug/lane). SERPINH1 (arrow) was detected using the purified Pab.)

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SERPINH1, Polyclonal Antibody (Cat# AAA28730)

Full Name

SERPINH1 Antibody (Center)

Gene Names

SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IF (Immunofluorescence) (AAA30921 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on Jurkat cell lysate using Phospho-CCR5(Ser349) Antibody.The lane on the left is treated with the antigen-specific peptide.)

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CCR5, Polyclonal Antibody (Cat# AAA30921)

Full Name

Phospho-CCR5(Ser349) Antibody

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IF (Immunofluorescence) (AAA30935 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of GR phosphorylation expression in Heatshock treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistchemistry) (AAA30935 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30935 at 1/100 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining human duodenum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30935 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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GR, Polyclonal Antibody (Cat# AAA30935)

Full Name

Phospho-GR (Ser211) Antibody

Gene Names

NR3C1; GR; GCR; GRL; GCCR; GCRST

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-STAT3 (Ser727) expression in Rat brain lysate)

IF (Immunofluorescence) (AAA31042 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA31042 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31042 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31042 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31042 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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STAT3, Polyclonal Antibody (Cat# AAA31042)

Full Name

Phospho-STAT3 (Ser727) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-c-Jun (Ser243) expression in various lysates)

IHC (Immunohistchemistry) (AAA30981 at 1/100 staining rat intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30981 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30981 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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c-Jun, Polyclonal Antibody (Cat# AAA30981)

Full Name

Phospho-c-Jun (Ser243) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-c-Jun (Tyr170) expression in various lysates)

IHC (Immunohistchemistry) (AAA30984 at 1/100 staining rat intestinal sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining rat lung sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30984 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30984 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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c-Jun, Polyclonal Antibody (Cat# AAA30984)

Full Name

Phospho-c-Jun (Tyr170) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry) (AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30994 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of IGF1R phosphorylation expression in Insulin treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA30994 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-IGF1R (Tyr1161) expression in various lysates)

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IGF1R, Polyclonal Antibody (Cat# AAA30994)

Full Name

Phospho-IGF1R (Tyr1161) Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human heart tissue using (AAA28740) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human uterus tissue using (AAA28740) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Application Data (Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry) (COL5A1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (COL5A1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).)

WB (Western Blot) (COL5A1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).)

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COL5A1, Polyclonal Antibody (Cat# AAA28740)

Full Name

COL5A1 Antibody (N-term)

Gene Names

COL5A1; EDSC

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates. MMP12 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with (lane 2) blocking peptide in 293 cell line lysate. MMP12 (arrow) was detected using the purified Pab;)

WB (Western Blot) (Anti-MMP12 Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-MMP12 Antibody at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry) (Staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

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MMP12, Polyclonal Antibody (Cat# AAA28713)

Full Name

MMP12 Antibody (C-term)

Gene Names

MMP12; ME; HME; MME; MMP-12

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry;clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot of APG16L (L92) Pab in mouse brain tissue lysate.)

WB (Western Blot) (APG16L Antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein arrow).)

WB (Western Blot) (Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Cos7, HEK293, MEF, and Hela cells, left to right, respectively.Data courtesy of Drs.Jiefei Geng and Dan Klionsky, University of Michigan.)

IF (Immunofluorescence) (Immunofluorescent analysis of U251 cells, using ATG16L Antibody.U251 cells(right) were treated with Chloroquine (50 uM,16h).AAA28729 was diluted at 1:25 dilution.Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)

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ATG16L, Polyclonal Antibody (Cat# AAA28729)

Full Name

ATG16L Antibody

Gene Names

ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L

Reactivity

Human, mouse

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ?g extracts of HeLa cells using 3 ?g DBI antibody (AAA28262). Western blot was performed from the immunoprecipitate using DBI antibody (AAA28262) at a dilution of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using DBI Rabbit pAb (AAA28262) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (AAA28262) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (AAA28262) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using DBI Rabbit pAb (AAA28262) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of DBI in paraffin-embedded rat ovary using DBI Rabbit pAb (AAA28262) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of DBI in paraffin-embedded rat liver using DBI Rabbit pAb (AAA28262) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using DBI Rabbit pAb (AAA28262) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of various lysates using DBI Rabbit pAb (AAA28262) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using DBI antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using DBI Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using DBI Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DBI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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DBI, Polyclonal Antibody (Cat# AAA28262)

Full Name

DBI Polyclonal Antibody

Gene Names

DBI; EP; ACBP; ACBD1; CCK-RP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (AAA28214) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (AAA28214) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse heart using CLUH antibody (AAA28214) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human stomach using CLUH antibody (AAA28214) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta using CLUH antibody (AAA28214) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CLUH antibody (AAA28214) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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CLUH, Polyclonal Antibody (Cat# AAA28214)

Full Name

CLUH Polyclonal Antibody

Gene Names

CLUH; CLU1; KIAA0664

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide)

WB (Western Blot) (Western blot analysis of lysates from HepG2 cells. The lane on the right is blocked with the synthesized peptide.)

WB (Western Blot) (Western Blot analysis of various cells)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/30,)

WB (Western Blot) (Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: HepG2 cells Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes)

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MET, Polyclonal Antibody (Cat# AAA29724)

Full Name

MET Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antigen Affinity Purification

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFkappaB-p65 (Phospho-Ser276) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NFkappaB-p65 (Phospho-Ser276) antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser276) antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p65 (Phospho-Ser276) Antibody (left) or the same antibody preincubated with blocking peptide #51011 (right).)

WB (Western Blot) (Western blot analysis of extract from Hela cells using NF-kappaB p65 (phospho-Ser276) antibody. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non- phospho peptide blocking peptides #61011. Lane 3: The antibody is preincubated with phospho peptide blocking peptides #51011.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NFkappaB-p65 (Phospho-Ser276) Antibody.)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA29703)

Full Name

NFkappaB-p65(Phospho-Ser276) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$305/0.05 mL $365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human seminoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human vascular cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human duodenum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31055 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31055 at 1/200 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human lymphoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human esophageal carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31055 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31055 at 1/200 staining human liver tissue cells by ICC/IF. Cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (1/200 in BSA) for 1 hour. An Alexa Fluor 488-conjugated Goat anti-rabbit antibody was used as the secondary.)

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LIMK1, Polyclonal Antibody (Cat# AAA31055)

Full Name

Phospho-LIMK1 (Thr508) Antibody

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-c-Jun (Thr239) expression in various lysates)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining rat intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30982 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30982 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30982 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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c-Jun, Polyclonal Antibody (Cat# AAA30982)

Full Name

Phospho-c-Jun (Thr239) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30991 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30991 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30991 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA30991 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of SP1 phosphorylation expression in A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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SP1, Polyclonal Antibody (Cat# AAA30991)

Full Name

Phospho-SP1 (Thr453) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Phospho-Tau (Ser404) expression in various lysates)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30999 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30999 at 1/200 staining Human esophagus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Human prostate tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30999 at 1/200 staining Human prostate tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Tau phosphorylation expression in UV treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Tau, Polyclonal Antibody (Cat# AAA30999)

Full Name

Phospho-Tau (Ser404) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry) (Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(F)IHC(F): Mouse Lung Tissue)

IHC (Immunohistochemistry) (Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(F)IHC(F): Rat Lung Tissue)

IHC (Immunohistochemistry) (Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemistry) (Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(P)IHC(P): Rat Lung Tissue)

IHC (Immunohistochemistry) (Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(P)IHC(P): Mouse Lung Tissue)

WB (Western Blot) (Anti- SFTP A1/2 Picoband antibody, AAA11644, Western blottingAll lanes: Anti SFTP (AAA11644) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Lung Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 26KD)

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SFTPA1/2, Polyclonal Antibody (Cat# AAA11644)

Full Name

Anti-SFTPA1/2 Antibody

Gene Names

SFTPA2; PSAP; PSPA; SP-A; SPA2; PSP-A; SFTP1; SPAII; COLEC5; SFTPA2B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Applications: Western BlotSample: 22RV1 cytosolic and membrane fractionsDetection: Enhanced chemiluminescence"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system.")

IHC (Immunohistochemistry) (Anti-NOX2/gp91phox antibody, AAA11515, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot) (Anti-NOX2/gp91phox antibody, AAA11515, Western blottingLane 1: HELA Cell LysateLane 2: JURKAT Cell LysateLane 3: MCF-7 Cell LysateLane 4: SMMC Cell Lysate)

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NOX2/gp91phox, Polyclonal Antibody (Cat# AAA11515)

Full Name

Anti-NOX2/gp91phox antibody

Gene Names

CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX

Reactivity

Reacts with Human , Rat
Predicted to work with: Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing Hela cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SiHa cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing SiHa cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 5. Western blot analysis of Calbindin using anti-Calbindin antibody (AAA11603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calbindin at approximately 37KD. The expected band size for Calbindin is at 30KD.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Calbindin using anti-Calbindin antibody (AAA11603). Calbindin was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (AAA11603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (AAA11603). Calbindin was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (AAA11603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (AAA11603). Calbindin was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (AAA11603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (AAA11603). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (AAA11603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Calbindin, Polyclonal Antibody (Cat# AAA11603)

Full Name

Anti-Calbindin Antibody

Gene Names

CALB1; CALB

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.