Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM (Flow Cytometry) (Figure 22. Flow Cytometry analysis of HepG2 cells using anti-HADH antibody (AAA19796).Overlay histogram showing HepG2 cells stained with AAA19796 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HADH Antibody (AAA19796, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 21. IF analysis of HADH using antiHADH antibody (AAA19796).HADH was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 20. IF analysis of HADH using anti-HADH antibody (AAA19796).HADH was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 15. IHC analysis of HADH using anti-HADH antibody (AAA19796).HADH was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HADH Antibody (AAA19796) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADH antigen affinity purified polyclonal antibody (#AAA19796) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HADH at approximately 34 kDa. The expected band size for HADH is at 34 kDa.)

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HADH, Polyclonal Antibody (Cat# AAA19796)

Full Name

Anti-HADH Antibody Picoband

Gene Names

HADH; HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: FUM knockout Hela cell lysate; Predicted MW: 55,50kd Observed MW: 50kd Primary Ab: 1ug/ml Rabbit Anti-Human FUM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Serum; Lane2: Human Hela Cells; Lane3: Porcine Liver Tissue.)

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Fumarase, Polyclonal Antibody (Cat# AAA21019)

Full Name

Fumarase (FUM) Polyclonal Antibody

Gene Names

FH; MCL; FMRD; LRCC; HLRCC; MCUL1

Reactivity

Human, Pig

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: SPTLC1 knockout Jurkat cell lysate; Predicted MW: 53kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Mouse SPTLC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Mouse.)

WB (Western Blot) (Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Mouse Brain Tissue; Lane4: Human Jurkat Cells.)

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Serine Palmitoyltransferase, Polyclonal Antibody (Cat# AAA21006)

Full Name

Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) Polyclonal Antibody

Gene Names

Sptlc1; Lcb1; C77762; AW552086; E030036H05

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $310/0.2 mL $475/0.5 mL $575/1 mL $1,515/5 mL $2,935/2x5 mL $4,275/3x5 mL $5,540/4x5 mL $6,750/5x5 mL

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WB (Western Blot) (Western blot analysis of ARSB in mouse lung tissue lysate with ARSB antibody at (A) 1 and (B) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of ARSB in human lung tissue with ARSB antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-ARSB / Arylsulfatase B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-ARSB / Arylsulfatase B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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ARSB / Arylsulfatase B, Polyclonal Antibody (Cat# AAA21268)

Full Name

Anti-ARSB / Arylsulfatase B Antibody (C-Terminus) IHC-plus

Gene Names

ARSB; ASB; G4S; MPS6

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Peptide Column Affinity Chromotography

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

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Knockout Validation (Knockout Validation: Lane 1: Wild-type HL60 cell lysate; Lane 2: GCA knockout HL60 cell lysate; Predicted MW: 24kd Observed MW: 27kd Primary Ab: 1ug/ml Rabbit Anti-Human GCA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Serum; Lane2: Human HL-60 Cells; Lane3: Mouse Sp2/0 Cells; Lane4: Rat Spleen Tissue.)

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Grancalcin, Polyclonal Antibody (Cat# AAA21015)

Full Name

Grancalcin (GCA) Polyclonal Antibody

Gene Names

GCA; GCL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$245/0.1 mL $305/0.2 mL $465/0.5 mL $565/1 mL $1,480/5 mL $2,865/2x5 mL $4,170/3x5 mL $5,405/4x5 mL $6,570/5x5 mL

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Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PALLD knockout Hela cell lysate; Predicted MW: 151,122~127,108kd Observed MW: 145kd Primary Ab: 1ug/ml Rabbit Anti-Human PALLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)

WB (Western Blot) (Western Blot: Sample: Human Hela Cells.)

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Palladin, Polyclonal Antibody (Cat# AAA21010)

Full Name

Palladin (PALLD) Polyclonal Antibody

Gene Names

PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Affinity Chromatography

Pricing

$240/0.1 mL $295/0.2 mL $445/0.5 mL $545/1 mL $1,410/5 mL $2,720/2x5 mL $3,965/3x5 mL $5,135/4x5 mL $6,250/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Tumor Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant TSPAN30, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human A375 Cells.)

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Tetraspanin 30, Polyclonal Antibody (Cat# AAA143108)

Full Name

Polyclonal Antibody to Tetraspanin 30 (TSPAN30)

Gene Names

CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30

Reactivity

Human, Pig

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohistochemistry) (Figure 4. IHC analysis of Factor D using anti-Factor D antibody (AAA124624).Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Factor D Antibody (AAA124624) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of Factor D using anti-Factor D antibody (AAA124624).Factor D was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Factor D Antibody (AAA124624) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Factor D using anti-Factor D antibody (AAA124624).Factor D was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Factor D Antibody (AAA124624) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Factor D using anti-Factor D antibody (AAA124624).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat RH35 cell lysates,Lane 2: rat PC-12 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.)

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Factor D, Polyclonal Antibody (Cat# AAA124624)

Full Name

Anti-Factor D Picoband antibody

Gene Names

Cfd; Df; Adn; EVE

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

WB (Western Blot) (Western Blot: Sample: Recombinant IFNg, Gallus.)

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Interferon Gamma (IFNg), Polyclonal Antibody (Cat# AAA131294)

Full Name

Polyclonal Antibody to Interferon Gamma (IFNg)

Reactivity

Chicken, Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded Stomach tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant MMP2, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human MCF7 Cells; Lane2: Human A431 Cells.)

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Matrix Metalloproteinase 2 (MMP2), Polyclonal Antibody (Cat# AAA131569)

Full Name

Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)

Gene Names

MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$170/0.1 mL $205/0.2 mL $305/0.5 mL $360/1 mL $905/5 mL $1,760/2x5 mL $2,570/3x5 mL $3,335/4x5 mL $4,055/5x5 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using CAB39 Polyclonal Antibody at dilution of 1:50)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer using CAB39 Polyclonal Antibody at dilution of 1:50)

WB (Western Blot) (Western Blot analysis of 293T and LoVo cell, Human brain malignant glioma tissue using CAB39 Polyclonal Antibody at dilution of 1:450)

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CAB39, Polyclonal Antibody (Cat# AAA166816)

Full Name

CAB39 Polyclonal Antibody

Gene Names

CAB39; MO25; CGI-66

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (BRSK1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRSK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (KIAA1811 Antibody (S685) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the KIAA1811 antibody detected the KIAA1811 protein (arrow).)

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BRSK1, Polyclonal Antibody (Cat# AAA284466)

Full Name

BRSK1 Antibody (C-term)

Gene Names

BRSK1; hSAD1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human lung cancer using PDLIM4 Polyclonal Antibody at dilution of 1:30)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using PDLIM4 Polyclonal Antibody at dilution of 1:30)

WB (Western Blot) (Western Blot analysis of A431 cell using PDLIM4 Polyclonal Antibody at dilution of 1:400)

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PDLIM4, Polyclonal Antibody (Cat# AAA168898)

Full Name

PDLIM4 Polyclonal Antibody

Gene Names

PDLIM4; RIL

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Uromodulin, Polyclonal Antibody (Cat# AAA142319)

Full Name

Polyclonal Antibody to Uromodulin (UMOD)

Gene Names

Umod; THP; Urehd1; urehr4

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of Netrin-1 antibody in paraffin-embedded human lung carcinoma tissue.)

WB (Western Blot) (Western blot analysis of lysate from COS7 cells, using Netrin-1 antibody.)

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Netrin-1, Polyclonal Antibody (Cat# AAA318467)

Full Name

Netrin-1 Antibody

Gene Names

NTN1; NTN1L

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:MouseTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Centaurin Alpha 2 (CENTa2), Polyclonal Antibody (Cat# AAA132349)

Full Name

Polyclonal Antibody to Centaurin Alpha 2 (CENTa2)

Gene Names

Adap2; Centa2

Reactivity

Mouse, Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Thymidine Phosphorylase (TP), Polyclonal Antibody (Cat# AAA131035)

Full Name

Polyclonal Antibody to Thymidine Phosphorylase (TP)

Gene Names

TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $255/0.2 mL $410/0.5 mL $490/1 mL $1,320/5 mL $2,565/2x5 mL $3,740/3x5 mL $4,860/4x5 mL $5,940/5x5 mL

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SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: AAA240111(NPHS1 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA240111(NPHS1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA240111(NPHS1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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NPHS1, Polyclonal Antibody (Cat# AAA240111)

Full Name

NPHS1 Antibody

Gene Names

NPHS1; CNF; NPHN; nephrin

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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FCM/FACS (Flow Cytometry) (EIF2C2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (EIF2C2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2C2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (EIF2C2 Antibody (N-term Q41) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).)

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EIF2C2, Polyclonal Antibody (Cat# AAA289596)

Full Name

EIF2C2 Antibody (N-term)

Gene Names

Ago2; Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493

Reactivity

Human (Predicted Reactivity: Bovine, Rabbit, Rat)

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:MouseTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Dipeptidyl Peptidase 9, Polyclonal Antibody (Cat# AAA143741)

Full Name

Polyclonal Antibody to Dipeptidyl Peptidase 9 (DPP9)

Gene Names

Dpp9; DPRP2; DPP IX; A330078I11; 6430584G11Rik

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using KSR1 Polyclonal Antibody at dilution 1:30)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using KSR1 Polyclonal Antibody at dilution 1:30)

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KSR1, Polyclonal Antibody (Cat# AAA168641)

Full Name

KSR1 Polyclonal Antibody

Gene Names

KSR1; KSR; RSU2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human CPB2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-CPB2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: k562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:48 kDa)

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Carboxypeptidase B2, Polyclonal Antibody (Cat# AAA256611)

Full Name

Anti-Carboxypeptidase B2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver cancer Tissue))

IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin- embedded rectum tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Pancreas lysate; Primary Ab: 2ug/ml Rabbit Anti-Human RNASE1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Ribonuclease A (RNASE1), Polyclonal Antibody (Cat# AAA133367)

Full Name

Polyclonal Antibody to Ribonuclease A (RNASE1)

Gene Names

RNASE1; RAC1; RIB1; RNS1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $255/0.2 mL $410/0.5 mL $490/1 mL $1,320/5 mL $2,565/2x5 mL $3,740/3x5 mL $4,860/4x5 mL $5,940/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on fromalin fixed paraffin- embedded spleen tissue))

IHC (Immunohistochemisry) (DAB staining on IHC-P;Samples: Mouse Cardiac Muscle Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse LTA4H AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot; Sample: Mouse Spleen lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse LTA4H AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot; Sample: Recombinant LTA4H, Mouse.)

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Leukotriene A4 Hydrolase, Polyclonal Antibody (Cat# AAA142023)

Full Name

Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H)

Reactivity

Mouse, Human, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Cytochrome P450 39A1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Cytochrome P450 39A1 expression in HeLa (A), HuvEc (B) whole cell lysates.)

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Cytochrome P450 39A1, Polyclonal Antibody (Cat# AAA104430)

Full Name

Anti-Cytochrome P450 39A1 Antibody

Reactivity

Human, Monkey, Pig

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: K562 cells, Hela cells, Jurkat cells, mouse heart tissue, Mouse spleen tissue, Primary antibody: AAA239216(NDUFS8 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA239216(NDUFS8 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA239216(NDUFS8 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200))

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NDUFS8, Polyclonal Antibody (Cat# AAA239216)

Full Name

NDUFS8 Antibody

Gene Names

NDUFS8; TYKY; CI-23k; CI23KD

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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FCM/FACS (Flow Cytometry) (MUC20 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of MUC20 Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (MUC20 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MUC20 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (MUC20 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MUC20 antibody detected the MUC20 protein (arrow).)

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MUC20, Polyclonal Antibody (Cat# AAA288268)

Full Name

MUC20 Antibody (C-term)

Gene Names

MUC20; MUC-20

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using GPR153 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using GPR153 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GPR153, Polyclonal Antibody (Cat# AAA318065)

Full Name

GPR153 Antibody

Gene Names

GPR153; PGR1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using FANCC antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat lung using FANCC antibody at dilution of 1:200 (x400 lens))

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FANCC antibody.)

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FANCC, Polyclonal Antibody (Cat# AAA168646)

Full Name

FANCC Antibody

Gene Names

FANCC; FA3; FAC; FACC

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human brain using PSMC1 Polyclonal Antibody at dilution of 1:25)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using PSMC1 Polyclonal Antibody at dilution of 1:25)

WB (Western Blot) (Western Blot analysis of 293T cell using PSMC1 Polyclonal Antibody at dilution of 1:350)

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PSMC1, Polyclonal Antibody (Cat# AAA169246)

Full Name

PSMC1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Tumor Necrosis Factor Alpha (TNFa), Polyclonal Antibody (Cat# AAA133538)

Full Name

Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Gene Names

TNF; TNFA; cTNF; TNLG1F

Reactivity

Human, Dog

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$200/0.1 mL $235/0.2 mL $370/0.5 mL $440/1 mL $1,180/5 mL $2,295/2x5 mL $3,355/3x5 mL $4,355/4x5 mL $5,320/5x5 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human TNIP1 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-TNIP1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:72 kDa(We are unsure as to the identity of these extra bands.))

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TNIP1, Polyclonal Antibody (Cat# AAA256180)

Full Name

Anti-TNIP1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VE-Cadherin (Phospho-Tyr731) Antibody)

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VE-Cadherin, Polyclonal Antibody (Cat# AAA318406)

Full Name

VE-Cadherin (Phospho-Tyr731) Antibody

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using AKT1/3 (Phospho-Tyr437/434) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AKT1/3 (Phospho-Tyr437/434) Antibody)

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AKT1/3, Polyclonal Antibody (Cat# AAA318283)

Full Name

AKT1/3 (Phospho-Tyr437/434) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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IHC (Immunohiostchemistry) (ARL8B Antibody-Anti-ARL8B/ARL8A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

WB (Western Blot) (ARL8B Antibody-ARL8B/ARL8A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

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ARL8B, Polyclonal Antibody (Cat# AAA162547)

Full Name

ARL8B Rabbit anti-Human Polyclonal (aa72-121) Antibody

Gene Names

ARL8B; Gie1; ARL10C

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Signal Transducer And Activator Of Transcription 5A (STAT5A), Polyclonal Antibody (Cat# AAA132755)

Full Name

Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5A (STAT5A)

Gene Names

STAT5A; MGF; STAT5

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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FCM/FACS (Flow Cytometry) (B-RAF Antibody (S445) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of B-RAF Antibody (S445) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S445) (RB10940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).)

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B-RAF, Polyclonal Antibody (Cat# AAA289044)

Full Name

B-RAF Antibody (S445)

Gene Names

BRAF; NS7; BRAF1; RAFB1; B-RAF1

Reactivity

Human (Predicted Reactivity: Chicken, Mouse)

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Fibronectin (FN), Polyclonal Antibody (Cat# AAA132816)

Full Name

Polyclonal Antibody to Fibronectin (FN)

Gene Names

FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$195/0.1 mL $215/0.2 mL $340/0.5 mL $390/1 mL $1,015/5 mL $1,980/2x5 mL $2,895/3x5 mL $3,755/4x5 mL $4,600/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Ataxin 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ataxin 2 expression in HeLa (A), U87MG (B), Jurkat (C) whole cell lysates.)

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Ataxin 2, Polyclonal Antibody (Cat# AAA104811)

Full Name

Anti-Ataxin 2 Antibody

Gene Names

ATXN2; ATX2; SCA2; ASL13; TNRC13

Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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Application Data

WB (Western Blot) (Western blot analysis of extracts of 293 cells, using Estrogen R ? antibody.)

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ESR1, Polyclonal Antibody (Cat# AAA167014)

Full Name

ESR1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CTBP2 Polyclonal Antibody at dilution 1:25)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using CTBP2 Polyclonal Antibody at dilution 1:25)

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CTBP2, Polyclonal Antibody (Cat# AAA169283)

Full Name

CTBP2 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.