At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
FCM/FACS (Flow Cytometry) (RAB10 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (RAB10 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB10 antibody detected the RAB10 protein (arrow).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colon cancer using SYN1 Polyclonal Antibody at dilution of 1:50)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using SYN1 Polyclonal Antibody at dilution of 1:50)
WB (Western Blot) (Western Blot analysis of A375 and 231 cell, 293T cell and Human hepatocellular carcinoma tissue using SYN1 Polyclonal Antibody at dilution of 1:550)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of MLH1 using anti-MLH1 antibody (AAA125961).MLH1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLH1 Antibody (AAA125961) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of MLH1 using anti-MLH1 antibody (AAA125961).MLH1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLH1 Antibody (AAA125961) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemisry) (Figure 3. IHC analysis of MLH1 using anti-MLH1 antibody (AAA125961).MLH1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLH1 Antibody (AAA125961) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry) (Figure 2. IHC analysis of MLH1 using anti-MLH1 antibody (AAA125961).MLH1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLH1 Antibody (AAA125961) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of MLH1 using anti-MLH1 antibody (AAA125961).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Daudi whole cell lysates,Lane 2: human T-47D whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human U251 whole cell lysates,Lane 6: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (#AAA125961) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85 kDa. The expected band size for MLH1 is at 85 kDa.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of AMBRA1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMBRA1(arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot) (The anti-LOK Pab is used in Western blot to detect LOK in Jurkat cell lysate.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of NCI-H292 cells using ABCC1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of ABCC1 antibody (C-term) in Ramos cell line lysates (35ug/lane). ABCC1 (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of lysates from K562 cells treated with forskolin 40nM 30' and 293 cells treated with PMA 125ng/ml 30', using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The lane on the right is blocked with the phospho peptide.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is blocked with the phospho peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: AAA239184(MVK Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AAA239184(MVK Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA239184(MVK Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SLC27A2 Polyclonal Antibody at dilution 1:20)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SLC27A2 Polyclonal Antibody at dilution 1:20)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CYP11B2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse adrenal gland using CYP11B2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat adrenal gland using CYP11B2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HEL cells using anti-TREML2 antibody (AAA126538).Overlay histogram showing HEL cells stained with AAA126538 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TREML2 Antibody (AAA126538, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of TREML2 using anti-TREML2 antibody (AAA126538).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEL whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: rat C6 whole cell lysates,Lane 4: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREML2 antigen affinity purified polyclonal antibody (#AAA126538) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TREML2 at approximately 70 kDa. The expected band size for TREML2 is at 35 kDa.)
IF (Immunofluorescence) (Immunofluorescence staining of DGCR8 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DGCR8 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
IHC (Immunohiostchemistry) (Immunochemical staining of human DGCR8 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-DGCR8 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:86 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohiostchemistry) (Immunochemical staining of human YARS2 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human YARS2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
FCM/FACS (Flow Cytometry) (PRR19 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (PRR19 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PRR19 antibody detected the PRR19 protein (arrow).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using RPL14 Polyclonal Antibody at dilution of 1:30(×200))
WB (Western Blot) (Western blot analysis of 293T cell lysates using RPL14 Polyclonal Antibody at dilution of 1:300)
WB (Western Blot) (Western blot analysis of lysates from COLO cells, using Rhodopsin Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rhodopsin Antibody. The picture on the right is blocked with the synthesized peptide.)
FCM/FACS (Flow Cytometry) (DPP8 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (DPP8 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DPP8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of DPP8 antibody (C-term) in 293 cell line lysates (35ug/lane). DPP8 (arrow) was detected using the purified Pab.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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