At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using FDXR Polyclonal Antibody at dilution of 1:110(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using FDXR Polyclonal Antibody at dilution of 1:110(×200))
WB (Western Blot) (Western blot analysis of HepG2 cell lysate using FDXR Polyclonal Antibody at dilution of 1:1000)
WB (Western Blot) (Western blot of extracts from A549 cells, using ST14 Antibody. The lane on the right is treated with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence of A549 cells, using ST14 Antibody. The picture on the right is treated with the synthesized peptide.)
IHC (Immunohiostchemistry) (Anti-ST14 / Matriptase antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry) (Anti-ST14 / Matriptase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohiostchemistry) (AAA325892 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hepg2 whole cell lysates, using VHL Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA325398 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from K562 cells, using BAG4 antibody.)
IF (Immunofluorescence) (AAA325398 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA241592(PNPT1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using AAA241592(PNPT1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
IP (Immunoprecipitation) (STX18 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-STX18 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STX18 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size :43 kDa)
WB (Western Blot) (Anti-STX18 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:43 kDa)
IP (Immunoprecipitation) (CALD1 was immunoprecipitated using:Lane A:0.5 mg HeLa 293T Whole Cell Lysate4 uL anti-CALD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CALD1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 93 kDaObserved band size :93 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of CALD1 in U2OS cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CALD1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot) (Anti-CALD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:93 kDaObserved band size:75 kDa)
IP (Immunoprecipitation) (HSPA2 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-HSPA2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HSPA2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 70 kDaObserved band size :70 kDa)
IHC (Immunohiostchemistry) (Immunochemical staining of human HSPA2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-HSPA2 rabbit polyclonal antibody at 1:2000dilutionLane A: MCF-7 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: A431 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation) (ADD1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-ADD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ADD1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 120 kDaObserved band size :120 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of ADD1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human ADD1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.)
IHC (Immunohiostchemistry) (Immunochemical staining of human ADD1 in human kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-ADD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:81 kDa)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using Histone H3 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H3 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using Histone H3 Polyclonal Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using Histone H3 Polyclonal Antibody at dilution of 1:1000.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using KIR2DL3/1/4/S4 Polyclonal Antibody at dilution 1:40)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer tissue using KIR2DL3/1/4/S4 Polyclonal Antibody at dilution 1:40)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse lung using TNNC2 Polyclonal antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human colon using TNNC2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using TNNC2 Polyclonal Antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GHDC Polyclonal Antibody at dilution of 1:90(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using GHDC Polyclonal Antibody at dilution of 1:90(×200))
IP (Immunoprecipitation) (PPID was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-PPID rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPID rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :43 kDa)
IHC (Immunohiostchemistry) (Immunochemical staining of human PPID in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot) (Anti-PPID rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:41 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot) (MED1/TRAP220 Antibody-Western blot analysis of TRAP220 expression in HeLa (A); HUVEC (B); Jurkat (C) whole cell lysates.)
ICC (Immunocytochemistry) (MED1/TRAP220 Antibody-Immunofluorescent analysis of TRAP220 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry) (RBPMS/Hermes Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot) (RBPMS/Hermes Antibody-Human muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot) (The anti-Phospho-Rad9-S272 Pab is used in Western blot to detect Phospho-Rad9-S272 in HL60 (left), Hela(middle), and Y79(right)tissue lysates.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human Lung tissue using SLC34A2 Polyclonal Antibody at dilution of 1:60)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer using SLC34A2 Polyclonal Antibody at dilution of 1:50)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using SLC34A2 Polyclonal Antibody at dilution of 1:50)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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