At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution 1:100)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution 1:100)
IF (Immunofluorescence) (Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry) (Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 μg/mL.)
WB (Western Blot) (Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse spleen using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal cancer using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CLDN6 Polyclonal Antibody at dilution of 1:25(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using CLDN6 Polyclonal Antibody at dilution of 1:25(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ELOC Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using ELOC Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCDC85C Polyclonal Antibody at dilution of 1:30(×200))
WB (Western Blot) (Western blot analysis of Human fetal intestines tissue and Human breast cancer tissue lysates using CCDC85C Polyclonal Antibody at dilution of 1:400)
IP (Immunoprecipitation) (PIR was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PIR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PIR rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size :36 kDa)
WB (Western Blot) (Anti-PIR rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:36 kDa)
FCM/FACS (Flow Cytometry) (SFRS1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot) (Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of SFRS1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.)
IHC (Immunohiostchemistry) (AAA326355 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of A549 whole cell lysates, using FXN Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts of HeLa, using PCBP2antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326740 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A549 cells using anti-CEP55 antibody (AAA128234).Overlay histogram showing A549 cells stained with AAA128234 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP55 Antibody (AAA128234, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot) (Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (AAA128234).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human Hacat whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (AAA128234) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.)
WB (Western Blot) (TIRC7/TCIRG1 Antibody-Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry) (TIRC7/TCIRG1 Antibody-Overlay histogram showing U-2 OS cells stained with TCIRG1 Antibody (C-Term)(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (TCIRG1 Antibody (C-Term), 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot) (Insulin Antibody-Anti-Insulin antibody (AAA162956, 20 ug/mL) yields a specific band on capillary Western analysis (Protein Simple, WES, 2-40 kDa separation system) in 0.25 mg/mL human insulin overexpression lysate.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver damage using Somatostatin Receptor 2 (SSTR2) Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using Somatostatin Receptor 2 (SSTR2) Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using Somatostatin Receptor 2 (SSTR2) Polyclonal Antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using GHRL Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using GHRL Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using ARF5 Polyclonal Antibody at dilution of 1:55(×200))
WB (Western Blot) (Western blot analysis of Raji cell Mouse brain tissue Hela A375 and 231 cell lysates using ARF5 Polyclonal Antibody at dilution of 1:400)
IF (Immunofluorescence) (Immunofluorescence of SESTD1 in Human Brain cells with SESTD1 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry) (Immunohistochemistry of SESTD1 in human brain tissue with SESTD1 antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of SESTD1 in rat brain tissue lysate with SESTD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot) (Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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