At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (HEK293 overexpressing HADH (RC201752) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and in the right panel (mock transfection in first and last lanes), tested by Origene.)
WB (Western Blot) ((0. 02ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) (TBX15 Antibody (N-term) (AAA288072) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the TBX15 antibody detected the TBX15 protein (arrow).)
IF (Immunofluorescence) (Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term)(AAA288072). HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). TBX15 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of SP1 Antibody (C-term P692) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry) (SP1 Antibody (C-term P692) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (SP1 Antibody (C-term P692) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (SP1 Antibody (C-term P692) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).)
FCM/FACS (Flow Cytometry) (DPP8 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (DPP8 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DPP8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of DPP8 antibody (C-term) in 293 cell line lysates (35ug/lane). DPP8 (arrow) was detected using the purified Pab.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human rectal cancer using TNFRSF1B Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using TNFRSF1B Polyclonal Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using TNFRSF1B Polyclonal Antibody at dilution of 1:200 (40x lens).)
IP (Immunoprecipitation) (LRRC59 was immunoprecipitated using:Lane A:0.5 mg HeLa HepG2 Jurkat A431 Whole Cell Lysate4 uL anti-LRRC59 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LRRC59 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa)
WB (Western Blot) (Anti-LRRC59 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohiostchemistry) (Immunochemical staining of human RHCG in human tonsil with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining of human RHCG in human esophagus with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ACAD9 Polyclonal Antibody at dilution of 1:10)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ACAD9 Polyclonal Antibody at dilution of 1:10)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using NT5C1A antibody.)
WB (Western Blot) (Western blot analysis of lysates from A549 cell line and human skeletal muscle tissue lysate(from left to right),using NT5C1A Antibody(AAA289224). AAA289224 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
FCM/FACS (Flow Cytometry) (MPV17 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with MPV17 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of MPV17 Antibody (N-term) in Hela cell line lysates (35ug/lane). MPV17 (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (IBTK Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (IBTK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IBTK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (IBTK Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IBTK antibody detected the IBTK protein (arrow).)
FCM/FACS (Flow Cytometry) (Overlay histogram showing A431 cells stained with AAA289114 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA289114, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot) (Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody (N-term). AAA289114 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot) (Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody (N-term). AAA289114 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). AAA289114 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using FDXR Polyclonal Antibody at dilution of 1:110(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using FDXR Polyclonal Antibody at dilution of 1:110(×200))
WB (Western Blot) (Western blot analysis of HepG2 cell lysate using FDXR Polyclonal Antibody at dilution of 1:1000)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GPR22 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using GPR22 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using GPR22 Polyclonal Antibody at dilution of 1:1000.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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