Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U251 cells using anti-TRAT1 antibody (AAA126591).Overlay histogram showing U251 cells stained with AAA126591 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAT1 Antibody (AAA126591, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of TRAT1 using anti-TRAT1 antibody (AAA126591).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MOLT-4 whole cell lysates,Lane 2: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAT1 antigen affinity purified polyclonal antibody (#AAA126591) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRAT1 at approximately 27-30 kDa. The expected band size for TRAT1 is at 21 kDa.)

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TRAT1, Polyclonal Antibody (Cat# AAA126591)

Full Name

Anti-TRAT1 Antibody Picoband

Gene Names

TRAT1; TRIM; TCRIM; HSPC062

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemisry) (DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human COQ10B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: ))

IHC (Immunohiostchemistry) (DAB staining on IHC-P;Samples: Human Colon Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human COQ10B AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: ))

WB (Western Blot) (Western Blot: Sample: Recombinant COQ10B, Human.)

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Coenzyme Q10 Homolog B, Polyclonal Antibody (Cat# AAA142825)

Full Name

Polyclonal Antibody to Coenzyme Q10 Homolog B (COQ10B)

Reactivity

Human, Rat, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$230/0.1 mL $295/0.2 mL $485/0.5 mL $590/1 mL $1,605/5 mL $3,120/2x5 mL $4,555/3x5 mL $5,915/4x5 mL $7,195/5x5 mL

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IF (Immunofluorescence) (AAA322567 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis Neurexin I using Jurkat whole cell lysates)

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Neurexin I, Polyclonal Antibody (Cat# AAA322567)

Full Name

Neurexin I Antibody

Gene Names

NRXN1; PTHSL2; SCZD17; Hs.22998

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326233 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF6465) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)

IHC (Immunohiostchemistry) (AAA326233 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from rat muscle, using TPH1 Antibody.)

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TPH1, Polyclonal Antibody (Cat# AAA326233)

Full Name

TPH1 Antibody

Gene Names

TPH1; TPRH; TRPH

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of MGC-803, using CTSE antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326802 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CTSE, Polyclonal Antibody (Cat# AAA326802)

Full Name

CTSE Antibody

Gene Names

CTSE; CATE

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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Application Data (Anti-VEGF Picoband antibody, AAA45665-3.jpgAll lanes: Anti-VEGF(AAA45665) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 40ugPredicted bind size: 27KDObserved bind size: 27KD)

IHC (Immunohiostchemistry) (Anti-VEGF Picoband antibody, AAA45665-2.JPGIHC(P): Human Placenta Tissue)

IHC (Immunohistochemistry) (Anti-VEGF Picoband antibody, AAA45665-1.JPGIHC(P): Human Lung Cancer Tissue)

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VEGF, Polyclonal Antibody (Cat# AAA45665)

Full Name

Anti-VEGF Antibody

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of DBF4 expression in Jurkat (A), HepG2 (B), SW480 (C) whole cell lysates.)

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DBF4, Polyclonal Antibody (Cat# AAA105352)

Full Name

Anti-DBF4 Antibody

Gene Names

DBF4; ASK; CHIF; DBF4A; ZDBF1

Reactivity

Human, Bovine, Pig

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using AAA240157(PIWIL2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA240157(PIWIL2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PIWIL2, Polyclonal Antibody (Cat# AAA240157)

Full Name

PIWIL2 Antibody

Gene Names

PIWIL2; CT80; HILI; mili; PIWIL1L

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using JunB(Ab-79) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Ab-79) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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JUNB, Polyclonal Antibody (Cat# AAA242067)

Full Name

JUNB (Ab-79) Antibody

Gene Names

JUNB; AP-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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SDS-PAGE (Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: AAA241712(SGPL1 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 second)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA241712(SGPL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241712(SGPL1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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SGPL1, Polyclonal Antibody (Cat# AAA241712)

Full Name

SGPL1 Antibody

Gene Names

SGPL1; SPL; S1PL

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of THP-1 cells using anti-GPCR TGR5/GPBAR1 antibody (AAA126191).Overlay histogram showing THP-1 cells stained with AAA126191 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR TGR5/GPBAR1 Antibody (AAA126191, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of GPCR TGR5/GPBAR1 using anti-GPCR TGR5/GPBAR1 antibody (AAA126191).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates,Lane 2: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR TGR5/GPBAR1 antigen affinity purified polyclonal antibody (#AAA126191) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPCR TGR5/GPBAR1 at approximately 45 kDa. The expected band size for GPCR TGR5/GPBAR1 is at 35 kDa.)

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GPCR TGR5/GPBAR1, Polyclonal Antibody (Cat# AAA126191)

Full Name

Anti-GPCR TGR5/GPBAR1 Antibody Picoband

Gene Names

GPBAR1; BG37; TGR5; M-BAR; GPCR19; GPR131

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse spinal cord using AXIN2 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using AXIN2 Polyclonal Antibody at dilution of 1:100 (40x lens).)

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AXIN2, Polyclonal Antibody (Cat# AAA177593)

Full Name

AXIN2 Polyclonal Antibody

Gene Names

AXIN2; AXIL; ODCRCS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U20S cells using anti-TMOD2 antibody (AAA127762).Overlay histogram showing U20S cells stained with AAA127762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMOD2 Antibody (AAA127762, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 2. IF analysis of TMOD2 and Tubulin beta using anti-TMOD2 antibody (AAA127762) and anti-Tubulin beta antibody (M05613-4).TMOD2 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-TMOD2 Antibody (AAA127762) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of TMOD2 using anti-TMOD2 antibody (AAA127762).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human SH-SY5Y whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMOD2 antigen affinity purified polyclonal antibody (#AAA127762) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TMOD2 at approximately 40 kDa. The expected band size for TMOD2 is at 40 kDa.)

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TMOD2, Polyclonal Antibody (Cat# AAA127762)

Full Name

Anti-TMOD2 Antibody Picoband

Gene Names

TMOD2; NTMOD; N-TMOD

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (AAA327213 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis of extracts from mouse kidney, using Catalase Antibody. Lane 1 was treated with the antigen-specific peptide.)

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Catalase, Polyclonal Antibody (Cat# AAA327213)

Full Name

Catalase Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA326390 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hepg2 whole cell lysates, using ALDH1A1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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ALDH1A1, Polyclonal Antibody (Cat# AAA326390)

Full Name

ALDH1A1 Antibody

Gene Names

ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA327422 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis STXBP1 using HeLa whole cell lysates)

IF (Immunofluorescence) (AAA327422 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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STXBP1, Polyclonal Antibody (Cat# AAA327422)

Full Name

STXBP1 Antibody

Gene Names

STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1

Reactivity

Human, Mouse, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer using MMP28 Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of Mouse heart tissue using MMP28 Polyclonal Antibody at dilution of 1:400)

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MMP28, Polyclonal Antibody (Cat# AAA166614)

Full Name

MMP28 Polyclonal Antibody

Gene Names

MMP28; MM28; MMP25; MMP-25; MMP-28; EPILYSIN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant SST, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Rat Heart Tissue; Lane2: Rat Stomach Tissue.)

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Somatostatin (SST), Polyclonal Antibody (Cat# AAA131647)

Full Name

Polyclonal Antibody to Somatostatin (SST)

Gene Names

SST; SMST

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $255/0.2 mL $410/0.5 mL $490/1 mL $1,320/5 mL $2,565/2x5 mL $3,740/3x5 mL $4,860/4x5 mL $5,940/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on IHC-P; Samples: Human Pancreas Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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CUB And Zona Pellucida Like Domains Protein 1 (CUZD1), Polyclonal Antibody (Cat# AAA133570)

Full Name

Polyclonal Antibody to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1)

Gene Names

CUZD1; ERG1; ERG-1; UO-44; ITMAP1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$230/0.1 mL $295/0.2 mL $485/0.5 mL $590/1 mL $1,605/5 mL $3,120/2x5 mL $4,555/3x5 mL $5,915/4x5 mL $7,195/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DEFA5 Polyclonal Antibody at dilution 1:15)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using DEFA5 Polyclonal Antibody at dilution 1:15)

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DEFA5, Polyclonal Antibody (Cat# AAA166249)

Full Name

DEFA5 Polyclonal Antibody

Gene Names

DEFA5; DEF5; HD-5

Reactivity

Human

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of Mouse lung using PRKCSH Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using PRKCSH Polyclonal Antibody at 1:1000 dilution.)

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PRKCSH, Polyclonal Antibody (Cat# AAA179403)

Full Name

PRKCSH Polyclonal Antibody

Gene Names

PRKCSH; PCLD; PLD1; G19P1; PKCSH; AGE-R2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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Application Data

Application Data

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USP29, Polyclonal Antibody (Cat# AAA303403)

Full Name

USP29 Antibody

Gene Names

USP29; HOM-TES-84/86

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purified

Pricing

$455/0.1 mL $890/2x0.1 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,870/5x0.1 mL

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WB (Western Blot) (Western blot analysis of K562 293T using FAT10 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

WB (Western Blot) (Western blot analysis of K562 using FAT10 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

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FAT10, Polyclonal Antibody (Cat# AAA318703)

Full Name

FAT10 Rabbit Polyclonal Antibody

Gene Names

UBD; FAT10; UBD-3; GABBR1

Reactivity

Human, Rat, Mouse

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of Hela cells using primary antibody diluted at 1:1000(4 degree C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25 degree C, 1 hour).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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PCNA, Polyclonal Antibody (Cat# AAA319927)

Full Name

PCNA Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$295/0.1 mL $845/0.5 mL $1,355/1 mL $2,635/2x1 mL $3,845/3x1 mL $4,990/4x1 mL $5,840/5x1 mL

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IP (Immunoprecipitation) (MAP2K2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-MAP2K2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MAP2K2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :47 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of MAP2K2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MAP2K2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

WB (Western Blot) (Anti-MAP2K2 rabbit polyclonal antibody at 1:2000 dilutionLane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa(We are unsure as to the identity of these extra bands.))

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MEK2, Polyclonal Antibody (Cat# AAA256582)

Full Name

Anti-MEK2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image of AAA243495 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA243495 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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ATF5, Polyclonal Antibody (Cat# AAA243495)

Full Name

ATF5 Antibody

Gene Names

ATF5; ATFX; HMFN0395

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of rat brain tissue, using CPLX1 antibody.)

IHC (Immunohiostchemistry) (AAA323016 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using CPLX1 antibody. Lane 1 was treated with the blocking peptide.)

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CPLX1, Polyclonal Antibody (Cat# AAA323016)

Full Name

CPLX1 Antibody

Gene Names

CPLX1; CPX1; CPX-I; EIEE63

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from A549 cells, using CDH7 antibody.The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA324221 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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CDH7, Polyclonal Antibody (Cat# AAA324221)

Full Name

CDH7 Antibody

Gene Names

CDH7; CDH7L1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using ACTN1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA326073 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326073 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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ACTN1, Polyclonal Antibody (Cat# AAA326073)

Full Name

ACTN1 Antibody

Gene Names

ACTN1; BDPLT15

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326680 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemisry) (AAA326680 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of PC-3, using TOLLIP antibody. The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of extracts from mouse muscle, using TOLLIP Antibody.)

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TOLLIP, Polyclonal Antibody (Cat# AAA326680)

Full Name

TOLLIP Antibody

Gene Names

TOLLIP; IL-1RAcPIP

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SGPL1 Polyclonal Antibody at dilution 1:40)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using SGPL1 Polyclonal Antibody at dilution 1:40)

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SGPL1, Polyclonal Antibody (Cat# AAA167310)

Full Name

SGPL1 Polyclonal Antibody

Gene Names

SGPL1; SPL; S1PL

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using NDUFA9 Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of Hela cell using NDUFA9 Polyclonal Antibody at dilution of 1:400)

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NDUFA9, Polyclonal Antibody (Cat# AAA167703)

Full Name

NDUFA9 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain using BMP4 Polyclonal Antibody at dilution of 1:25)

WB (Western Blot) (Western Blot analysis of Mouse intestinumm tenue tissue using BMP4 Polyclonal Antibody at dilution of 1:450)

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BMP4, Polyclonal Antibody (Cat# AAA169369)

Full Name

BMP4 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-C11orf24 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateC11orf24 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)

IHC (Immunohistochemistry) (Rabbit Anti-C11orf24 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult LiverObserved Staining: Membrane but only in connective tissue cells in the interlobular septum, very low tissue distributionPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

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C11orf24, Polyclonal Antibody (Cat# AAA200027)

Full Name

C11orf24 antibody - N-terminal region

Gene Names

C11orf24; DM4E3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using SPARCL1 Polyclonal Antibody at dilution 1:30)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SPARCL1 Polyclonal Antibody at dilution 1:30)

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SPARCL1, Polyclonal Antibody (Cat# AAA170550)

Full Name

SPARCL1 Polyclonal Antibody

Gene Names

SPARCL1; SC1; MAST9; PIG33; MAST 9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of 3T3 cells with PAK5 Polyclonal Antibody.)

WB (Western Blot) (Western Blot analysis of Mouse brain tissue with PAK5 Polyclonal Antibody.)

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PAK5, Polyclonal Antibody (Cat# AAA172138)

Full Name

PAK5 Polyclonal Antibody

Gene Names

PAK7; PAK5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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WB (Western Blot) (Western Blot analysis of Mouse heart tissue using ENTPD1 Polyclonal Antibody at dilution of 1/200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using ENTPD1 Polyclonal Antibody at dilution of 1/25)

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ENTPD1, Polyclonal Antibody (Cat# AAA173350)

Full Name

ENTPD1 Polyclonal Antibody

Gene Names

ENTPD1; CD39; SPG64; ATPDase; NTPDase-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ESR2 antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ESR2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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ESR2, Polyclonal Antibody (Cat# AAA37331)

Full Name

ESR2 Polyclonal Antibody

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of JK cells using anti-MIS12 antibody (AAA127408).Overlay histogram showing JK cells stained with AAA127408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIS12 Antibody (AAA127408, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of MIS12 using anti-MIS12 antibody (AAA127408).MIS12 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MIS12 Antibody (AAA127408) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of MIS12 using anti-MIS12 antibody (AAA127408).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human Hacat whole cell lysates,Lane 6: human U251 whole cell lysates,Lane 7: human SH-SY5Y whole cell lysates,Lane 8: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIS12 antigen affinity purified polyclonal antibody (#AAA127408) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIS12 at approximately 25 kDa. The expected band size for MIS12 is at 24 kDa.)

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MIS12, Polyclonal Antibody (Cat# AAA127408)

Full Name

Anti-MIS12 Antibody Picoband

Gene Names

MIS12; MTW1; hMis12; KNTC2AP; 2510025F08Rik

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot analysis of extracts of K562, using CDC25C antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326312 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326312 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CDC25C, Polyclonal Antibody (Cat# AAA326312)

Full Name

CDC25C Antibody

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA327106 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA327106 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of MCF7 cell lysate, using TACR1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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TACR1, Polyclonal Antibody (Cat# AAA327106)

Full Name

TACR1 Antibody

Gene Names

TACR1; SPR; NK1R; NKIR; TAC1R

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (VDAC2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) HIER using 10 mM sodium citrate buffer pH 6.0)

IHC (Immunohiostchemistry) (VDAC2 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) HIER using 10 mM sodium citrate buffer pH 6.0)

IHC (Immunohistochemistry) (VDAC2 Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) HIER using 10 mM sodium citrate buffer pH 6.0)

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VDAC2, Polyclonal Antibody (Cat# AAA163314)

Full Name

VDAC2 Rabbit anti-Human Polyclonal (C-terminal domain of human) Antibody

Gene Names

VDAC2; POR

Reactivity

Human

Applications

Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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IHC (Immunohiostchemistry) (NR0B1/DAX1 Antibody-Anti-DAX1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

WB (Western Blot) (NR0B1/DAX1 Antibody-NR0B1/DAX-1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

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NR0B1/DAX1, Polyclonal Antibody (Cat# AAA162484)

Full Name

NR0B1/DAX1 Rabbit anti-Human Polyclonal (aa359-408) Antibody

Gene Names

NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Western blot analysis of Anti-PEX11B Antibody at a 1:500 dilution. Lane 1: MCF-7 cell lysate. Lane 2: Raw264.7 cell lysate. Lane 3: PC12 cell lysate.)

IHC (Immunohistochemistry) (Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

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PEX11B, Polyclonal Antibody (Cat# AAA162169)

Full Name

Anti-PEX11B Antibody (aa132-177) IHC-plus

Gene Names

PEX11B; PEX14B; PEX11-BETA

Reactivity

Mouse, Rat, Human

Applications

Immunoprecipitation, Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IF (Immunofluorescence) (Detection of H2AK9ac by immunofluorescence. U2OS cells were stained with H2AK9ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK9ac antibody staining. Right panel: merge.)

DB (Dot Blot) (Specificity data: Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys9 pAb for acetyl Lys9 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide acetylated at Lys5 of H2A. Lane 2: Unmodified Lys5 peptide. Lane 3: Peptide acetylated at Lys9 of H2A. Lane 4: Unmodified Lys9 peptide.)

WB (Western Blot) (Histone H2A acetyl Lys9 pAb tested by Western blot. Western blot probed with Histone H2A acetyl Lys9 polyclonal antibody (1:5,000 dilution). Lane 1: 20 ng recombinant histone H2A. Lane 2: 5 ug acid extract of HeLa cells. Lane 3: 5 ug acid extract of HeLa cells treated with sodium butyrate.)

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Histone H2AK9ac, Polyclonal Antibody (Cat# AAA59828)

Full Name

Histone H2AK9ac antibody (pAb)

Gene Names

HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101

Reactivity

Human, Wide Range Predicted

Applications

Western Blot, Immunofluorescence, Dot Blot

Pricing

$250/0.01 mL $705/0.2 mL $1,375/2x0.2 mL $2,005/3x0.2 mL $2,605/4x0.2 mL $3,015/5x0.2 mL

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IHC (Immunohiostchemistry) (IHC image of AAA243536 diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Mouse brain tissue, Mouse liver tissueAll lanes: HSPD1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62, 18 kDaObserved band size: 62 kDa)

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HSPD1, Polyclonal Antibody (Cat# AAA243536)

Full Name

HSPD1 Antibody

Gene Names

HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

>95%, Protein G Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using AAA240145(PAX9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240145(PAX9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PAX9, Polyclonal Antibody (Cat# AAA240145)

Full Name

PAX9 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

SDS-PAGE (Gel: 8%SDS-PAGE lysate40 ¦Œg, Lane 1-4: LoVo and A549 cell Mouseliver tissue and Rat liver tissue lysatesPrimary antibody: 46511£¨CLCN7 Antibody) at dilution 1/300Secondary antibody: Goat anti rabbit IgG at 1/8000 dilutionExposure time: 20 seconds)

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CLCN7, Polyclonal Antibody (Cat# AAA310373)

Full Name

CLCN7 Antibody

Gene Names

CLCN7; CLC7; CLC-7; OPTA2; OPTB4; PPP1R63

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (FOXF2 Antibody) at dilution 1/100. (Original magnification: -200)

Application Data (Gel: 8%SDS-PAGELysate: 10 ugLane : Mouse small intestines tissue lysatePrimary antibody: (FOXF2 Antibody) at dilution 1/800Secondary antibody: (HRP-conjugated Goat anti rabbit IgG) at 1/20000 dilutionExposure time: 10 seconds (Detected by Chemiluminescence Imager))

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FOXF2, Polyclonal Antibody (Cat# AAA308029)

Full Name

FOXF2 Antibody

Gene Names

FOXF2; FKHL6; FREAC2; FREAC-2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.