At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using GPR103 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using GPR103 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4 degree over night)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
IF (Immunofluorescence) (AAA322099 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohiostchemistry) (AAA322099 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of HSP70 expression in Hela cells)
IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using Anti-LYPLA2 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohistochemistry) (Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ERCC5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Lanes:Lane 1: 30ug human 293T lysateLane 2: 30ug human BEL7402 cell lysateLane 3: 30ug human SMMC772 lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit-HRP Anti-rabbit-HRPSecondary Antibody Dilution:1:10,000Gene Name:TNS4 aSubmitted by:Dr Frankie Ko Chi Fat, Lo-Kong Chan, Irene O.L. Ng, Judy Wai Ping Yam; Department of Pathology, The University of Hong Kong)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using BLOC1S6 Polyclonal Antibody at dilution of 1:95(×200))
WB (Western Blot) (Western blot analysis of 293T K562 and HepG2 cell lysates using BLOC1S6 Polyclonal Antibody at dilution of 1:650)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HepG2 cells using anti-VPS15/PIK3R4 antibody (AAA127389).Overlay histogram showing HepG2 cells stained with AAA127389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS15/PIK3R4 Antibody (AAA127389, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (AAA127389).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS15/PIK3R4 antigen affinity purified polyclonal antibody (#AAA127389) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VPS15/PIK3R4 at approximately 150 kDa. The expected band size for VPS15/PIK3R4 is at 153 kDa.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HEL cells using anti-SVOPL antibody (AAA127898).Overlay histogram showing HEL cells stained with AAA127898 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SVOPL Antibody (AAA127898, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot) (Figure 1. Western blot analysis of SVOPL using anti-SVOPL antibody (AAA127898).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human MOLT-4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SVOPL antigen affinity purified polyclonal antibody (#AAA127898) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SVOPL at approximately 37 kDa. The expected band size for SVOPL is at 54,37 kDa.)
IHC (Immunohiostchemistry) (Anti- PAPP A Picoband antibody, AAA46565, IHC(P)IHC(P): Human Placenta Tissue)
WB (Western Blot) (Anti- PAPP A Picoband antibody, AAA46565, Western blottingAll lanes: Anti PAPP A (AAA46565) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HT1080 Whole Cell Lysate at 40ugLane 3: SKOV Whole Cell Lysate at 40ugLane 4: 22RV1 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: MM231 Whole Cell Lysate at 40ugPredicted bind size: 181KDObserved bind size: 181KD)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A431 cells using anti-CDC7 antibody (AAA126121).Overlay histogram showing A431 cells stained with AAA126121 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC7 Antibody (AAA126121, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of CDC7 using anti-CDC7 antibody (AAA126121).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC7 antigen affinity purified polyclonal antibody (#AAA126121) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC7 at approximately 64 kDa. The expected band size for CDC7 is at 64 kDa.)
FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U87 cells using anti-Gastrin/GAST antibody (AAA126323).Overlay histogram showing U87 cells stained with AAA126323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (AAA126323, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-Gastrin/GAST antibody (AAA126323).Overlay histogram showing Caco-2 cells stained with AAA126323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (AAA126323, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot) (Figure 1. Western blot analysis of Gastrin/GAST using anti-Gastrin/GAST antibody (AAA126323).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysates,Lane 2: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastrin/GAST antigen affinity purified polyclonal antibody (#AAA126323) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Gastrin/GAST at approximately 20 kDa. The expected band size for Gastrin/GAST is at 11 kDa.)
WB (Western Blot) (HEK293 overexpressing HAVCR2 (RC209440) and probed (mock transfection in first lane), tested by Origene.)
WB (Western Blot) ((0.3ug/ml) staining of human tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IHC (Immunohiostchemistry) (AAA60852 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot) ((0.3ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
SDS-PAGE (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse lung tissue, Primary antibody: AAA240025(OLR1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA240025(OLR1 Antibody) at dilution 1/12, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA240025(OLR1 Antibody) at dilution 1/12, on the right is treated with synthetic peptide. (Original magnification: ×200))
WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using OCT2 antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot) (Western blot analysis of extracts from COS7 cells, using OCT2 antibody.)
IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using OCT2 antibody.)
WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using Claudin 2 Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Claudin 2 Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF12138 1:200) and mouse antibeta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from Hela, using TELO2 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot) (Western blot analysis of extracts of A431 cells, using TELO2 antibody.)
IHC (Immunohiostchemistry) (AAA322963 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from HepG2, using TELO2 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot) (Western blot analysis of extracts of HEK-293 cells, using GEFT antibody.)
IHC (Immunohistochemistry) (AAA323227 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326772 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts of LoVo, using PDCD4 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326772 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hepg2 whole cell lysates, using ALDH4A1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326793 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of SW480, using PRKACB antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326978 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemistry) (AAA326978 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from rat liver, rat brain, using CKI-alpha Antibody.)
WB (Western Blot) (Western blot analysis of extracts from HeLa cells, using CKI-alpha antibody.)
IF (Immunofluorescence) (AAA323888 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemistry) (AAA323888 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis on mouse brain lysates using VPS72 Antibody)
IHC (Immunohiostchemistry) (AAA323997 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA323997 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence) (AAA324033 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from HT-29 cells, using MAGI2 antibody.)
IHC (Immunohistochemisry) (AAA326514 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326514 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of Hela cell lysate, using IFI16 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) ((2ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) ((2ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot) ((1ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Application Data (Detection of Mouse anti Bovine CD28 in a flow cytometric analysis of bovine peripheral blood monocytes using Goat anti Mouse IgG H+L:FITC)
Application Data (Detection of Mouse anti Equine CD44 in a flow cytometric analysis of equine peripheral blood lymphocytes using Goat anti Mouse H+L:FITC)
Application Data (Detection of Mouse anti Bovine CD28 in a flow cytometric analysis of bovine peripheral blood monocytes using Goat anti Mouse IgG H+L:FITC)
Application Data (Detection of Rabbit anti Human α-adaptin in a flow cytometric analysis of mouse bone marrow cells using Goat anti Mouse IgG H+L:FITC)
Application Data (Detection of Mouse anti Rabbit CD44 in a flow cytometric analysis of rabbit peripheral blood lymphocytes using Goat anti Rabbit IgG H+L:FITC)
IHC (Immunohiostchemistry) (Anti-Collagen IV Picoband antibody, AAA45744-2.JPGIHC(P): Human Lung Cancer Tissue)
Application Data (Anti-Collagen IV Picoband antibody, AAA45744-1.jpgAll lanes: Anti Collagen IV (AAA45744) at 0.5ug/mlWB: Recombinant Human Collagen IV Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
IHC (Immunohiostchemistry) (Immunohistochemistry of caspase-14 in human liver tissue with caspase-14 antibody at 2.5 μg/mL.)
WB (Western Blot) (Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using FAM19A4 Polyclonal Antibody at dilution of 1:65(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using FAM19A4 Polyclonal Antibody at dilution of 1:65(×200))
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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