Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-IDI2 antibody (AAA127701).Overlay histogram showing Caco-2 cells stained with AAA127701 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDI2 Antibody (AAA127701, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of IDI2 using anti-IDI2 antibody .Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDI2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDI2 at approximately 27 kDa. The expected band size for IDI2 is at 27 kDa.)

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IDI2, Polyclonal Antibody (Cat# AAA127701)

Full Name

Anti-IDI2 Antibody Picoband

Gene Names

IDI2; IPPI2

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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DB (Dot Blot) (Histone H4K8ac antibody (pAb) tested by Dot blot. Dot blot analysis was used to confirm the specificity of the acetyl Lys8 histone H4 antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 0.1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: acetyl-Lys8 peptide. Lane 3: acetyl-Lys12 peptide. Lane 4: acetyl-Lys16 peptide. Lane 5: acetyl-Lys20 peptide.)

WB (Western Blot) (Histone H4K8ac antibody (pAb) tested by Western Blot. HeLa nuclear extract (20 ug per lane) probed with the Histone H4 acetyl Lys8 antibody (0.5 ug per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.)

IF (Immunofluorescence) (Histone H4K8ac antibody (pAb) tested by immunofluorescence. HeLa cells stained with Histone H4K8ac antibody (green) at a 1 ug/ml dilution. Tubulin staining was done with our alpha Tubulin antibody, (red) at a 1:1,000 dilution.)

ChIP (Chromatin Immunoprecipitation) (Histone H4K8ac antibody (pAb) tested by Chromatin IP. ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ug of Histone H4 acetyl Lys8 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 gene or the GAPDH gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

ChIP (Chromatin Immunoprecipitation) (Histone H4K8ac antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Histone H4K8ac binding sites. The image shows binding across a region of chromosome 12.)

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Histone H4K8ac, Polyclonal Antibody (Cat# AAA59978)

Full Name

Histone H4K8ac antibody (pAb)

Gene Names

HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116

Reactivity

Human, Mouse, Wide Range Predicted

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Dot Blot, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Purity

Protein A Chromatography

Pricing

$250/0.01 mg $725/0.1 mg $1,415/2x0.1 mg $2,070/3x0.1 mg $2,680/4x0.1 mg $3,100/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of ATAD3A in Daudi cells with ATAD3A antibody at 20 μg/mL.)

ICC (Immunocytochemistry) (Immunocytochemistry of ATAD3A in Daudi cells with ATAD3A antibody at 20 μg/mL.)

WB (Western Blot) (Western blot analysis of ATAD3A in Daudi cell lysate with ATAD3A antibody at 1 μg/mL .)

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ATAD3A, Polyclonal Antibody (Cat# AAA39670)

Full Name

ATAD3A Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ATAD3A Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of TEM2 in Human Colon cells with TEM2 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of TEM2 in human colon tissue with TEM2 antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of TEM2 in human colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.)

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TEM2, Polyclonal Antibody (Cat# AAA39710)

Full Name

TEM2 Antibody

Gene Names

RASD2; Rhes; TEM2; MGC:4834

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

TEM2 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.)

ICC (Immunocytochemistry) (Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.)

WB (Western Blot) (Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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ZC3HAV1, Polyclonal Antibody (Cat# AAA40392)

Full Name

ZC3HAV1 Antibody

Gene Names

ZC3HAV1; ZAP; ZC3H2; ARTD13; PARP13; FLB6421; ZC3HDC2

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

ZC3HAV1 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti-Annexin A3 antibody, AAA45051, IHC(P)IHC(P): Human Mammary Cancer Tissue)

WB (Western Blot) (Anti-Annexin A3 antibody, AAA45051, Western blottingAll lanes: Anti Annexin A3 (AAA45051) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: Rat Skeletal Muscle Tissue Lysate at 50ugLane 4: Rat Lung Tissue Lysate at 50ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: SMMC Whole Cell Lysate at 40ugLane 8: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 36KDObserved bind size: 36KD)

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Annexin A3, Polyclonal Antibody (Cat# AAA45051)

Full Name

Anti-Annexin A3 antibody

Gene Names

Anxa3; LC3; Anx3; LRRGT00047

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Anti-Tuberin Picoband antibody, AAA45660-5.jpgIHC(P): Human Mammary Cancer Tissue)

IHC (Immunohistochemistry) (Anti-Tuberin Picoband antibody, AAA45660-4.jpgIHC(P): Mouse Brain Tissue)

IHC (Immunohistochemisry) (Anti-Tuberin Picoband antibody, AAA45660-3.jpgIHC(P): Rat Brain Tissue)

Application Data (Anti-Tuberin Picoband antibody, AAA45660-2.jpgAll lanes: Anti Tuberin (AAA45660) at 0.5ug/mlLane 1: U20S Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 201KDObserved bind size: 201KD)

Application Data (Anti-Tuberin Picoband antibody, AAA45660-1.jpgAll lanes: Anti Tuberin (AAA45660) at 0.5ug/mlWB: Recombinant Human Tuberin Protein 0.5ngPredicted bind size: 41KDObserved bind size: 41KD)

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Tuberin, Polyclonal Antibody (Cat# AAA45660)

Full Name

Anti-Tuberin Antibody

Gene Names

TSC2; LAM; TSC4; PPP1R160

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemisry) (Anti-PRLR antibody, AAA45452, IHC(P)IHC(P): Rat Testis Tissue)

IHC (Immunohiostchemistry) (Anti-PRLR antibody, AAA45452, IHC(P)IHC(P): Human Mammary Cancer Tissue)

WB (Western Blot) (Figure 1. Western blot analysis of PRLR using anti- PRLR antibody (AAA45452).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PRLR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.)

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Prolactin Receptor, Polyclonal Antibody (Cat# AAA45452)

Full Name

Anti-Prolactin Receptor antibody

Gene Names

Prlr; Pr-1; Pr-3; AI987712; Prlr-rs1

Reactivity

Reacts with: Human, Rat
Predicted to work with: Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Anti- IKB beta Picoband antibody, AAA46351,IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemisry) (Anti- IKB beta Picoband antibody, AAA46351,IHC(P)IHC(P): Mouse Intestine Tissue)

IHC (Immunohiostchemistry) (Anti- IKB beta Picoband antibody, AAA46351,IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot) (Anti- IKB beta Picoband antibody, AAA46351, Western blottingAll lanes: Anti IKB beta (AAA46351) at 0.5ug/mlLane 1: Mouse Kidney Tissue Lysate at 50ugLane 2: RH35 Whole Cell Lysate at 40ugLane 3: NRK Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD)

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IKB beta, Polyclonal Antibody (Cat# AAA46351)

Full Name

Anti-IKB beta Antibody

Gene Names

NFKBIB; IKBB; TRIP9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

WB (Western Blot) (Western blot analysis of NOC3L expression in HEK293T (A), mouse liver (B), rat kidney (C) whole cell lysates.)

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NOC3L, Polyclonal Antibody (Cat# AAA105403)

Full Name

Anti-NOC3L Antibody

Gene Names

NOC3L; AD24; FAD24; C10orf117

Reactivity

Human, Mouse, Rat, Bovine, Pig

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of TSHZ1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of TSHZ1 expression in HEK293T (A), Raw264.7 (B) whole cell lysates.)

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TSHZ1, Polyclonal Antibody (Cat# AAA106141)

Full Name

Anti-TSHZ1 Antibody

Gene Names

TSHZ1; CAA; TSH1; NY-CO-33; SDCCAG33

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of human heart tissue, using LMOD3 antibody.)

IHC (Immunohistochemistry) (AAA322884 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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LMOD3, Polyclonal Antibody (Cat# AAA322884)

Full Name

LMOD3 Antibody

Gene Names

LMOD3; NEM10

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from mouse heart, using ARF6 Antibody.)

WB (Western Blot) (Western blot analysis of A431 using ART6 antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA325952 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA325952 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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ARF6, Polyclonal Antibody (Cat# AAA325952)

Full Name

ARF6 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA323761 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells using TALL-2 antibody.)

IHC (Immunohistochemistry) (AAA323761 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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TALL-2, Polyclonal Antibody (Cat# AAA323761)

Full Name

TALL-2 Antibody

Gene Names

TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from rat spleen, using PTHR1 Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using PTHR1 antibody.The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA325529 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA325529 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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PTHR1, Polyclonal Antibody (Cat# AAA325529)

Full Name

PTHR1 Antibody

Gene Names

PTH1R; PFE; PTHR; PTHR1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/30.)

Application Data (Gel: 8%SDS-PAGE Lysates (from left to right): Mouse brain and human fetal brain tissue, hela, Raw264.7, NIH/3T3 and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

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NDUFS3, Polyclonal Antibody (Cat# AAA305531)

Full Name

NDUFS3 Antibody

Gene Names

NDUFS3; CI-30

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/20.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue using at dilution 1/20.)

WB (Western Blot) (Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: Hela cell Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

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FASN, Polyclonal Antibody (Cat# AAA309122)

Full Name

FASN Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Ab-695) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells using PKCth(Ab-695) Antibody and the same antibody preincubated with blocking peptide.)

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PRKCQ, Polyclonal Antibody (Cat# AAA242210)

Full Name

PRKCQ (Ab-695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of HEK-293 cells, using alpha Adaptin antibody.)

IHC (Immunohistochemistry) (AAA323171 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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alpha Adaptin, Polyclonal Antibody (Cat# AAA323171)

Full Name

alpha Adaptin Antibody

Gene Names

AP2A1; ADTAA; CLAPA1; AP2-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of OR51M1 Antibody expression in SiHa cells lysates.The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of OR51M1 expression in SiHa cell line lysates ;, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA323620 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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OR51M1, Polyclonal Antibody (Cat# AAA323620)

Full Name

OR51M1 Antibody

Gene Names

OR51M1; OR11-40; HOR5'Beta7

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA323652 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA323652 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Bcl6 using HUVEC whole cell lysates)

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BCL6, Polyclonal Antibody (Cat# AAA323652)

Full Name

BCL6 Antibody

Gene Names

BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA323911 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA323911 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using EFNA5 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells, using EFNA5 antibody.)

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EFNA5, Polyclonal Antibody (Cat# AAA323911)

Full Name

EFNA5 Antibody

Gene Names

EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (SEMA3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEMA3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (SEMA3G Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SEMA3G antibody detected the SEMA3G protein (arrow).)

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SEMA3G, Polyclonal Antibody (Cat# AAA288908)

Full Name

SEMA3G Antibody (Center)

Gene Names

SEMA3G; sem2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (AAA321815 at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis of Caspase 4 expression in mouse brain and lung.)

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Caspase 4, Polyclonal Antibody (Cat# AAA321815)

Full Name

Caspase 4 Antibody

Gene Names

CASP4; TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA321376 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of C2C12 cells treated with CIP, using Phospho-PTPalpha (Tyr798/789) Antibody.)

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PTPalpha, Polyclonal Antibody (Cat# AAA321376)

Full Name

Phospho-PTPalpha (Tyr798/789) Antibody

Gene Names

PTPRA; LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of S-100A10 antibody in paraffin-embedded human lung carcinoma tissue.)

WB (Western Blot) (Western blot analysis of lysate from COLO cells, using S-100A10 antibody.)

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S-100A10, Polyclonal Antibody (Cat# AAA318489)

Full Name

S-100A10 Antibody

Gene Names

S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with etoposide 25uM 24h, using JAK2 (Phospho-Tyr570) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using JAK2 (Phospho-Tyr570) Antibody)

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JAK2, Polyclonal Antibody (Cat# AAA318337)

Full Name

JAK2 (Phospho-Tyr570) Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using VIPR2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using VIPR2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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VIPR2, Polyclonal Antibody (Cat# AAA318235)

Full Name

VIPR2 Antibody

Gene Names

VIPR2; VPAC2; VPAC2R; VIP-R-2; VPCAP2R; PACAP-R3; DUP7q36.3; PACAP-R-3; C16DUPq36.3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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Stathmin, Polyclonal Antibody (Cat# AAA319488)

Full Name

Stathmin (Phospho-Ser38) Antibody

Gene Names

STMN2; SCG10; SCGN10

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322128 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Chk1 expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322128 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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CHK1, Polyclonal Antibody (Cat# AAA322128)

Full Name

CHK1 Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA322383 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of FAK expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA322383 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 63 degree C)

IF (Immunofluorescence) (AAA322383 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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FAK, Polyclonal Antibody (Cat# AAA322383)

Full Name

FAK Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of CD31 expression in mouse lung tissue lysates.)

WB (Western Blot) (Western blot analysis of PECAM-1 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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CD31, Polyclonal Antibody (Cat# AAA322240)

Full Name

CD31 Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322254 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322254 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of ATF1 expression in COLO205 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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ATF1, Polyclonal Antibody (Cat# AAA322254)

Full Name

ATF1 Antibody

Gene Names

ATF1; TREB36; EWS-ATF1; FUS/ATF-1

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using ASNS Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer using ASNS Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of 231 and NIH/3T3 cell using ASNS Polyclonal Antibody at dilution of 1:800)

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ASNS, Polyclonal Antibody (Cat# AAA166730)

Full Name

ASNS Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:MouseTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Myeloperoxidase (MPO), Polyclonal Antibody (Cat# AAA132150)

Full Name

Polyclonal Antibody to Myeloperoxidase (MPO)

Gene Names

Mpo; mKIAA4033

Reactivity

Mouse, Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $265/0.2 mL $415/0.5 mL $500/1 mL $1,360/5 mL $2,645/2x5 mL $3,860/3x5 mL $5,010/4x5 mL $6,080/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Lung lysate; Primary Ab: 2ug/ml Rabbit Anti-Human RALBP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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RalA Binding Protein 1 (RALBP1), Polyclonal Antibody (Cat# AAA131139)

Full Name

Polyclonal Antibody to RalA Binding Protein 1 (RALBP1)

Gene Names

RALBP1; RIP1; RLIP1; RLIP76

Reactivity

Human, Mouse, Rat, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using KIFC1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse testis using KIFC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using KIFC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat testis using KIFC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

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KIFC1, Polyclonal Antibody (Cat# AAA177618)

Full Name

KIFC1 Polyclonal Antibody

Gene Names

KIFC1; HSET; KNSL2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PTGES3 Polyclonal Antibody at dilution 1:35)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer tissue using PTGES3 Polyclonal Antibody at dilution 1:35)

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PTGES3, Polyclonal Antibody (Cat# AAA169260)

Full Name

PTGES3 Polyclonal Antibody

Gene Names

PTGES3; P23; TEBP; cPGES

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colon cancer using PLEKHG6 Polyclonal Antibody at dilution of 1:20)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using PLEKHG6 Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of 293T cell using PLEKHG6 Polyclonal Antibody at dilution of 1:400)

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PLEKHG6, Polyclonal Antibody (Cat# AAA167375)

Full Name

PLEKHG6 Polyclonal Antibody

Gene Names

PLEKHG6; MyoGEF

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using SAA4 Polyclonal Antibody at dilution of 1:35(×200))

WB (Western Blot) (Western blot analysis of Human normal stomach tissue lysate using SAA4 Polyclonal Antibody at dilution of 1:350)

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SAA4, Polyclonal Antibody (Cat# AAA176146)

Full Name

SAA4 Polyclonal Antibody

Gene Names

SAA4; CSAA; C-SAA

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human appendix using PML Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using PML Polyclonal Antibody at 1:1000 dilution.)

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PML, Polyclonal Antibody (Cat# AAA179536)

Full Name

PML Polyclonal Antibody

Gene Names

PML; MYL; RNF71; PP8675; TRIM19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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Application Data (Anti-Cathepsin D Picoband antibody, AAA45687-3.jpgAll lanes: Anti-Cathepsin D(AAA45687) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Mouse Thymus Tissue Lysate at 40ugLane 4: NEURO Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)

IHC (Immunohiostchemistry) (Anti-Cathepsin D Picoband antibody, AAA45687-2.JPGIHC(P): Mouse Liver Tissue)

IHC (Immunohistochemistry) (Anti-Cathepsin D Picoband antibody, AAA45687-1.JPGIHC(P): Mouse Kidney Tissue)

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Cathepsin D, Polyclonal Antibody (Cat# AAA45687)

Full Name

Anti-Cathepsin D Antibody

Gene Names

Ctsd; CD; CatD

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight550) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HEL cells using anti-SEL1L antibody (AAA126301).Overlay histogram showing HEL cells stained with AAA126301 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEL1L Antibody (AAA126301, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of SEL1L using anti-SEL1L antibody (AAA126301).SEL1L was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-SEL1L Antibody (AAA126301) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of SEL1L using anti-SEL1L antibody (AAA126301).SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SEL1L Antibody (AAA126301) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of SEL1L using anti-SEL1L antibody (AAA126301).SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SEL1L Antibody (AAA126301) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SEL1L using anti-SEL1L antibody (AAA126301).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: rat pancreas tissue lysates,Lane 4: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody (#AAA126301) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SEL1L at approximately 100 kDa. The expected band size for SEL1L is at 89 kDa.)

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SEL1L, Polyclonal Antibody (Cat# AAA126301)

Full Name

Anti-SEL1L Antibody Picoband

Gene Names

SEL1L; SEL1L1; PRO1063; SEL1-LIKE

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GRM2, Polyclonal Antibody (Cat# AAA61526)

Full Name

Goat anti-GRM2 Antibody

Gene Names

GRM2; GLUR2; mGlu2; GPRC1B; MGLUR2

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Rabbit

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) (HEK293 overexpressing KU70 (RC204048) and probed (mock transfection in first lane), tested by Origene.)

WB (Western Blot) ((0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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KU70/XRCC6, Polyclonal Antibody (Cat# AAA61238)

Full Name

Goat anti-KU70/XRCC6 Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embeded human mammary gland using AAA81385 at dilution of 1:50)

WB (Western Blot) (All lanes: Rho GDP-dissociation inhibitor 1 antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 24,19 kDaObserved band size: 40 kDa)

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Rho GDP-dissociation inhibitor 1, Polyclonal Antibody (Cat# AAA81385)

Full Name

Rabbit anti-human Rho GDP-dissociation inhibitor 1 polyclonal Antibody

Gene Names

ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$235/0.05 mg $345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,490/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA81429 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of AAA81429 diluted at 1:600 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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Histone H2B type 1-C/E/F/G/I, Polyclonal Antibody (Cat# AAA81429)

Full Name

Rabbit anti-human Histone H2B type 1-C/E/F/G/I polyclonal Antibody

Gene Names

HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8

Reactivity

Human

Applications

Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$235/0.05 mg $345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,490/5x0.1 mg

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: 293T cells, hepG2 cells, A549 cells, Primary antibody: AAA240976(CKAP4 Antibody) at dilution 1/1250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240976(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA240976(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CKAP4, Polyclonal Antibody (Cat# AAA240976)

Full Name

CKAP4 Antibody

Gene Names

CKAP4; p63; CLIMP-63; ERGIC-63

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human colon cancer tissue, Primary antibody: AAA240504(PRKAR1B Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA240504(PRKAR1B Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA240504(PRKAR1B Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PRKAR1B, Polyclonal Antibody (Cat# AAA240504)

Full Name

PRKAR1B Antibody

Gene Names

PRKAR1B; PRKAR1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.