Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry)

Dilution Info

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IgG, Polyclonal Antibody (Cat# AAA59443)

Full Name

IgG

Reactivity

Human, Canine, Feline

Pricing

$215/5 Slides $215/3 mL (RTU) $240/0.1 mL (Concentrate) $280/0.5 mL (Concentrate) $280/7 mL (RTU) $340/1 mL (Concentrate) $400/15 mL (RTU) $780/2x15 mL (RTU) $1,130/3x15 mL (RTU) $1,325/5x1 mL (Concentrate) $1,465/4x15 mL (RTU) $1,615/5x15 mL (RTU)

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human placenta using AAA119690 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using AAA119690 at dilution 1:100)

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Bone marrow stromal antigen 2, Polyclonal Antibody (Cat# AAA119690)

Full Name

Rabbit anti-human Bone marrow stromal antigen 2 polyclonal Antibody(BST2)

Gene Names

BST2; CD317; TETHERIN

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Mouse Acvr2b Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Acvr2b antibody detected the Acvr2b protein (arrow).)

WB (Western Blot) (Mouse Acvr2b Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Acvr2b antibody detected the Acvr2b protein (arrow).)

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Acvr2b, Polyclonal Antibody (Cat# AAA288616)

Full Name

Mouse Acvr2b Antibody (Center)

Gene Names

Acvr2b; ActRIIB

Reactivity

Human, mouse

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat spleen using DPP3 Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat spinal cord using DPP3 Antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DPP3 antibody.)

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DPP3, Polyclonal Antibody (Cat# AAA311460)

Full Name

DPP3 Polyclonal Antibody

Gene Names

DPP3; DPPIII

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded humanbrain tissue reacted with Phospho-PUM1-Y83.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of PUM1 (arrow) using PUM1 Antibody (Y83). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM1 gene (Lane 2) (Origene Technologies).)

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PUM1, Polyclonal Antibody (Cat# AAA288373)

Full Name

PUM1 Antibody (Y83)

Gene Names

PUM1; PUMH; HSPUM; PUMH1; PUML1

Reactivity

Human (Predicted Reactivity: Chicken, Mouse)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 47256(TCF25 Antibody) at dilution 1/40.(Original magnification: 200))

IHC (Immunohiostchemistry) (The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 47256(TCF25 Antibody) at dilution 1/40.(Original magnification: 200))

SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Hela cell, Jurkat cell, NIH/3T3 cell, Human fetal brain tissue, 293T cell, K562 cell lysates, Primary antibody:47256(TCF25 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

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TCF25, Polyclonal Antibody (Cat# AAA310530)

Full Name

TCF25 Antibody

Gene Names

TCF25; Hulp1; NULP1; FKSG26; PRO2620; hKIAA1049

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of 293 cells using COL6A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of COL6A1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of COL6A1 antibody (C-term) in Y79 cell line lysates (35ug/lane). COL6A1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody (C-term). AAA289808 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

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COL6A1, Polyclonal Antibody (Cat# AAA289808)

Full Name

COL6A1 Antibody (C-term)

Gene Names

COL6A1; OPLL

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human brain cancer tissue using at dilution 1/50.)

Application Data (Gel: 8%SDS-PAGE Lysate: 40ug K562 cell Primary antibody: 1/283 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

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AARS, Polyclonal Antibody (Cat# AAA301542)

Full Name

AARS Antibody

Gene Names

AARS; CMT2N; EIEE29

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using DRD5 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Op9 cell lysate, using DRD5 antibody.)

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DRD5, Polyclonal Antibody (Cat# AAA306333)

Full Name

DRD5 antibody

Gene Names

DRD5; DBDR; DRD1B; DRD1L2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Ab-64) antibody.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Ab-64) antibody.)

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4E-BP1, Polyclonal Antibody (Cat# AAA306497)

Full Name

4E-BP1 (Ab-64) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 2.5 ug/mL.)

WB (Western Blot) (Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.)

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PD-1, Polyclonal Antibody (Cat# AAA307033)

Full Name

PD-1 Antibody

Gene Names

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using MCL1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MCL1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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MCL1, Polyclonal Antibody (Cat# AAA317273)

Full Name

MCL1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from A549 cells, using TAF5 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TAF5 Antibody. The picture on the right is blocked with the synthesized peptide.)

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TAF5, Polyclonal Antibody (Cat# AAA317536)

Full Name

TAF5 Antibody

Gene Names

TAF5; TAF2D; TAFII100; TAFII-100; TAF(II)100

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293, COLO, and HT-29 cells, using ABCC13 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ABCC13 Antibody. The picture on the right is blocked with the synthesized peptide.)

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ABCC13, Polyclonal Antibody (Cat# AAA317943)

Full Name

ABCC13 Antibody

Gene Names

ABCC13; PRED6; ABCC13P; C21orf73

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using AGTR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using AGTR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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AGTR1, Polyclonal Antibody (Cat# AAA318073)

Full Name

AGTR1 Antibody

Gene Names

AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using GABBR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using GABBR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GABBR1, Polyclonal Antibody (Cat# AAA318092)

Full Name

GABBR1 Antibody

Gene Names

GABBR1; GB1; GPRC3A; GABABR1; GABBR1-3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohistochemisry) (AAA322015 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA322015 staining U87-MG cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Claudin 11 expression in Mouse brain extract.The lane on the left is treated with the antigen-specific peptide.)

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Claudin 11, Polyclonal Antibody (Cat# AAA322015)

Full Name

Claudin 11 Antibody

Gene Names

CLDN11; OSP; OTM

Reactivity

Human, Mouse, Rat, Cow

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from A549 cells, using PP2A-alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PP2A-alpha Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells, using PP2A-alpha Antibody. The picture on the right is blocked with the synthesized peptide.)

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PP2A-alpha, Polyclonal Antibody (Cat# AAA316795)

Full Name

PP2A-alpha Antibody

Gene Names

PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of rat muscle cells using KLF12 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100)

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KLF12, Polyclonal Antibody (Cat# AAA318721)

Full Name

KLF12 Rabbit Polyclonal Antibody

Gene Names

KLF12; AP2REP; AP-2rep; HSPC122

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of A-Myb using LOVO whole cell lysates)

IF (Immunofluorescence) (AAA322474 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse muscle, using A-Myb Antibody.)

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A-Myb, Polyclonal Antibody (Cat# AAA322474)

Full Name

A-Myb Antibody

Gene Names

MYBL1; AMYB; A-MYB

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA322573 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

IF (Immunofluorescence) (AAA322573 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF9133 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

IHC (Immunohiostchemistry) (AAA322573 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using NMUR1 Antibody.)

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NMUR1, Polyclonal Antibody (Cat# AAA322573)

Full Name

NMUR1 Antibody

Gene Names

NMUR1; FM3; FM-3; GPC-R; GPR66; NMU1R; (FM-3)

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence staining of TP53BP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TP53BP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human TP53BP1 in human prostate with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

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TP53BP1, Polyclonal Antibody (Cat# AAA256477)

Full Name

Anti-TP53BP1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1(Phospho-Ser957) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with UV using SMC1(Phospho-Ser957) Antibody.)

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SMC1A, Polyclonal Antibody (Cat# AAA243112)

Full Name

Phospho-SMC1A (Ser957) Antibody

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunochemical staining of human ECHDC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human ECHDC2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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ECHDC2, Polyclonal Antibody (Cat# AAA256222)

Full Name

Anti-ECHDC2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohistochemisry) (AAA324058 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formal dehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells using RIN3 antibody.)

IF (Immunofluorescence) (AAA324058 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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RIN3, Polyclonal Antibody (Cat# AAA324058)

Full Name

RIN3 Antibody

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326641 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326641 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat, using CYP19A1 antibody. The lane on the left is treated with the antigen-specific peptide.)

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CYP19A1, Polyclonal Antibody (Cat# AAA326641)

Full Name

CYP19A1 Antibody

Gene Names

CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326757 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat, using HMGB1antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326757 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of various tissue sample, using HMGB1 Antibody.)

IHC (Immunohistochemistry) (AAA326757 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HMGB1, Polyclonal Antibody (Cat# AAA326757)

Full Name

HMGB1 Antibody

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using GRP antibody (A6380) at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GRP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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GRP, Polyclonal Antibody (Cat# AAA37602)

Full Name

GRP Polyclonal Antibody

Gene Names

GRP; BN; GRP-10; proGRP; preproGRP

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of NIBRIN (CT) in human lung tissue with NIBRIN (CT) antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of NIBRIN in mouse lung tissue lysate with NIBRIN antibody at 1 μg/mL.)

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NIBRIN, Polyclonal Antibody (Cat# AAA39916)

Full Name

NIBRIN Antibody

Gene Names

NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

NIBRIN Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of MD-1 in human spleen tissue with MD-1 antibody at 2 μg/mL.)

WB (Western Blot) (Western blot analysis of MD-1 in Daudi cell lysate with MD-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

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MD-1, Polyclonal Antibody (Cat# AAA39853)

Full Name

MD-1 Antibody

Gene Names

LY86; MD1; MD-1; MMD-1; dJ80N2.1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

MD-1 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti-Lamin B2 antibody, AAA45083, IHC(P)IHC(P): Rat Intestine Tissue)

WB (Western Blot) (Anti-Lamin B2 antibody, AAA45083, Western blottingLane 1: HELA Cell LysateLane 2: U87 Cell Lysate)

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Lamin B2, Polyclonal Antibody (Cat# AAA45083)

Full Name

Anti-Lamin B2 antibody

Gene Names

LMNB2; LMN2; LAMB2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μg/ml.Red: S1PR2 Antibody (8229)Blue: DAPI staining)

IHC (Immunohiostchemistry) (Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.)

WB (Western Blot) (Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.)

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S1PR2, Polyclonal Antibody (Cat# AAA40843)

Full Name

S1PR2 Antibody

Gene Names

S1PR2; EDG5; H218; LPB2; S1P2; AGR16; EDG-5; DFNB68; Gpcr13

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Fig: 2 Formalin-fixed, paraffin-embedded mouse pancreas stained for TRAF2 expression using at 1: 2000. Hematoxylin eosin counterstain. A1 is a high magnification of A. Expression of TRAF2 is seen in the pancreatic islets.)

IHC (Immunohistochemistry) (Fig: 1 Formalin-fixed, paraffin-embedded mouse testis/epidymis stained for TRAF2 expression using at 1: 2000. Hematoxylin eosin counterstain.)

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TRAF-2, Polyclonal Antibody (Cat# AAA78363)

Full Name

Polyclonal Antibody to TRAF-2

Gene Names

TRAF2; TRAP; TRAP3; MGC:45012

Reactivity

Mouse

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Serum

Pricing

$365/0.05 mL $705/2x0.05 mL $1,030/3x0.05 mL $1,335/4x0.05 mL $1,460/5x0.05 mL

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HEL cells using anti-SEPT6/SEPTIN6 antibody (AAA126584).Overlay histogram showing HEL cells stained with AAA126584 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT6/SEPTIN6 Antibody (AAA126584, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of ANA-1 cells using anti-SEPT6/SEPTIN6 antibody (AAA126584).Overlay histogram showing ANA-1 cells stained with AAA126584 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT6/SEPTIN6 Antibody (AAA126584, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (AAA126584).SEPT6/SEPTIN6 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SEPT6/SEPTIN6 Antibody (AAA126584) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (AAA126584).SEPT6/SEPTIN6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SEPT6/SEPTIN6 Antibody (AAA126584) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (AAA126584).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human MOLT-4 whole cell lysates,Lane 3: rat thymus tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT6/SEPTIN6 antigen affinity purified polyclonal antibody (#AAA126584) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SEPT6/SEPTIN6 at approximately 55 kDa. The expected band size for SEPT6/SEPTIN6 is at 50 kDa.)

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SEPT6/SEPTIN6, Polyclonal Antibody (Cat# AAA126584)

Full Name

Anti-SEPT6/SEPTIN6 Antibody Picoband

Gene Names

SEPT6; SEP2; SEPT2; RP5-876A24.2

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Figure 5. IHC analysis of PIPOX using anti-PIPOX antibody (AAA127656).PIPOX was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PIPOX Antibody (AAA127656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PIPOX using anti-PIPOX antibody (AAA127656).PIPOX was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PIPOX Antibody (AAA127656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of PIPOX using anti-PIPOX antibody (AAA127656).PIPOX was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PIPOX Antibody (AAA127656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of PIPOX using anti-PIPOX antibody (AAA127656).PIPOX was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PIPOX Antibody (AAA127656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PIPOX using anti-PIPOX antibody (AAA127656).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: rat liver tissue lysates,Lane 3: mouse liver tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIPOX antigen affinity purified polyclonal antibody (#AAA127656) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIPOX at approximately 40 kDa. The expected band size for PIPOX is at 44 kDa.)

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PIPOX, Polyclonal Antibody (Cat# AAA127656)

Full Name

Anti-PIPOX Antibody Picoband

Gene Names

PIPOX; LPIPOX

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot of extracts from SKOV3 cells, using Cyclin A1 Antibody. The lane on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Anti-CCNA1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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CCNA1 / Cyclin A1, Polyclonal Antibody (Cat# AAA51426)

Full Name

Rabbit Polyclonal (IgG) to Human CCNA1 / Cyclin A1

Gene Names

CCNA1; CT146

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (Western blot of HAVCR1/TIM-1 in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate.)

IHC (Immunohistochemistry) (Anti-TIM-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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HAVCR1 / KIM-1, Polyclonal Antibody (Cat# AAA50844)

Full Name

Rabbit Polyclonal (IgG) to Human HAVCR1 / KIM-1

Gene Names

HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1

Reactivity

Human, Mouse
Predicted Reactivity: Gorilla, Monkey (at least 90% immunogen sequence identity)

Applications

Western Blot, Immunohistochemistry

Purity

Protein G Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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WB (Western Blot) (Western blot analysis of extracts from rat muscle, using SQLE antibody. Lane 1 was treated with the blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts of A549 cells, using SQLE antibody.)

IHC (Immunohistochemistry) (AAA322890 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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SQLE, Polyclonal Antibody (Cat# AAA322890)

Full Name

SQLE Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA326376 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using HMBS Antibody. The lane on the left is treated with the antigen-specific peptide.)

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HMBS, Polyclonal Antibody (Cat# AAA326376)

Full Name

HMBS Antibody

Gene Names

HMBS; UPS; PBGD; PORC; PBG-D

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using PDGFR beta (Phospho-Tyr740) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PDGFR beta (Phospho-Tyr740) Antibody)

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PDGFR beta, Polyclonal Antibody (Cat# AAA318371)

Full Name

PDGFR beta (Phospho-Tyr740) Antibody

Gene Names

PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells treated with UV, using HSP27 (Phospho-Ser15) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP27 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.)

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HSP27, Polyclonal Antibody (Cat# AAA316511)

Full Name

HSP27 (Phospho-Ser15) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser602) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser602) Antibody. The picture on the right is blocked with the phospho peptide.)

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B-RAF, Polyclonal Antibody (Cat# AAA316297)

Full Name

B-RAF (Phospho-Ser602) Antibody

Gene Names

BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29 cells, treated with calyculinA 50ng/ml 30', using CBP Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CBP Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using CBP Antibody. The picture on the right is blocked with the synthesized peptide.)

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CBP, Polyclonal Antibody (Cat# AAA318016)

Full Name

CBP Antibody

Gene Names

CREBBP; CBP; RSTS; KAT3A; RSTS1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from A549 cells, using COX41 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using COX41 Antibody. The picture on the right is blocked with the synthesized peptide.)

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COX41, Polyclonal Antibody (Cat# AAA317717)

Full Name

COX41 Antibody

Gene Names

COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa and HepG2 cells, using OR5M1/5M10 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using OR5M1/5M10 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR5M1/5M10, Polyclonal Antibody (Cat# AAA318255)

Full Name

OR5M1/5M10 Antibody

Gene Names

OR5M1; OST050; OR11-208

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from rat brain, using OR51A1P Antibody. Lane 1 was treated with the blocking peptide.)

WB (Western Blot) (Western blot analysis of OR51A1P expression in MDA-MB453 cell line lysate ;, The lane on the left is treated with the antigen-specific peptide.)

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OR51A1P, Polyclonal Antibody (Cat# AAA323552)

Full Name

OR51A1P Antibody

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human THOC7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC7 antibody AAA213138 (lot AAA213138-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of human THOC7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THOC7 antibody AAA213138 (lot AAA213138-1) used for IP at 6 ug per reaction. THOC7 was also immunoprecipitated by rabbit anti-THOC7 antibodies and BL20424. For blotting immunoprecipitated THOC7, AAA213138 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

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THOC7, Polyclonal Antibody (Cat# AAA213138)

Full Name

Rabbit anti-THOC7 Antibody, Affinity Purified

Gene Names

THOC7; fSAP24; NIF3L1BP1

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $605/0.1 mg $940/5x0.01 mg $1,170/2x0.1 mg $1,710/3x0.1 mg $2,220/4x0.1 mg $2,475/5x0.1 mg

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0.1ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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XRCC4-like factor/NHEJ1, Polyclonal Antibody (Cat# AAA61185)

Full Name

Goat anti-XRCC4-like factor/NHEJ1 Antibody

Gene Names

NHEJ1; XLF

Reactivity

Tested: Human; Expected from sequence similarity: Human, Dog

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohiostchemistry) ((2ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot) (HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)

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FOXC2, Polyclonal Antibody (Cat# AAA60938)

Full Name

Goat anti-FOXC2 Antibody

Gene Names

FOXC2; LD; MFH1; MFH-1; FKHL14

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.