Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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Application Data

IF (Immunofluorescence) (AAA323658 staining HepG2 cells by ICC/IF.)

WB (Western Blot) (Western blot analysis of CD11b using K562 whole cell lysates)

IHC (Immunohistochemistry) (AAA323658 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CD11b, Polyclonal Antibody (Cat# AAA323658)

Full Name

CD11b Antibody

Gene Names

ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin. (Thermo Fisher Scientific).

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human PQBP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody AAA212021 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)

IHC (Immunohistochemistry) (Detection of human and mouse PQBP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 (Cat. No. AAA212021) used at a dilution of 1:200 (1 ug/ml) Detection: DAB)

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PQBP1, Polyclonal Antibody (Cat# AAA212021)

Full Name

Rabbit anti-PQBP1 Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $605/0.02 mg $940/5x0.002 mg $1,170/2x0.02 mg $1,710/3x0.02 mg $2,220/4x0.02 mg $2,475/5x0.02 mg

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FCM/FACS (Flow Cytometry) (COL5A2 Antibody (N-term)(AAA286923) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of COL5A2 Antibody (N-term)(AAA286923) in mouse lung tissue lysates (35ug/lane). COL5A2 (arrow) was detected using the purified Pab.)

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COL5A2, Polyclonal Antibody (Cat# AAA286923)

Full Name

COL5A2 Antibody (N-term)

Gene Names

COL5A2; EDSC

Reactivity

Human, mouse

Applications

Flow Cytometry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46694(UCN3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))

SDS-PAGE (Gel: 12%SDS-PAGE lysate: 40 ¦Œg, Lane: HT-29 cell lysate, Primary antibody: 46694£¨UCN3 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)

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UCN3, Polyclonal Antibody (Cat# AAA310475)

Full Name

UCN3 Antibody

Gene Names

UCN3; SCP; SPC; UCNIII

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of PPM1F (arrow) using PPM1F Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).)

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PPM1F, Polyclonal Antibody (Cat# AAA289363)

Full Name

PPM1F Antibody (C-term)

Gene Names

PPM1F; CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr172) Antibody. The picture on the right is blocked with the phospho peptide.)

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AMPK alpha, Polyclonal Antibody (Cat# AAA316054)

Full Name

AMPK alpha (Phospho-Thr172) Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HUVEC cells, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.)

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Arachidonate 5 Lipoxygenase, Polyclonal Antibody (Cat# AAA316290)

Full Name

Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody

Gene Names

ALOX5; 5-LO; 5LPG; LOG5; 5-LOX

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from mouse brain, using UBQLN4 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using UBQLN4 Antibody. The picture on the right is blocked with the synthesized peptide.)

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UBQLN4, Polyclonal Antibody (Cat# AAA317969)

Full Name

UBQLN4 Antibody

Gene Names

UBQLN4; A1U; A1Up; UBIN; CIP75; C1orf6

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide.)

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Histone H3, Polyclonal Antibody (Cat# AAA317996)

Full Name

Histone H3 (Acetyl-Lys23) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with TSA 400uM 24h, using Lys-Acetylated Proteins Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide.)

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Lys-Acetylated Proteins, Polyclonal Antibody (Cat# AAA318006)

Full Name

Lys-Acetylated Proteins Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MCF7 cells using Histone H3 Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of MCF-7 cells using Histone H3 Polyclonal Antibody)

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Histone H3, Polyclonal Antibody (Cat# AAA303071)

Full Name

Histone H3 Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from K562 cells (Lane 2), using Gastrin antiobdy. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells, using Gastrin antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Gastrin antibody.)

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Gastrin, Polyclonal Antibody (Cat# AAA302111)

Full Name

Gastrin Antibody

Gene Names

GAST; GAS

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29 and COS7 cells, using CNR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CNR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using CNR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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CNR1, Polyclonal Antibody (Cat# AAA318080)

Full Name

CNR1 Antibody

Gene Names

CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa, HUVEC, Jurkat, HepG2, and MCF-7 cells, using OR1D4/5 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using OR1D4/5 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR1D4/5, Polyclonal Antibody (Cat# AAA318251)

Full Name

OR1D4/5 Antibody

Gene Names

OR1D5; OR1D4; OR17-2; C17orf2; OR17-30; OR17-31

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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RN169, Polyclonal Antibody (Cat# AAA319802)

Full Name

RN169 Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$265/0.04 mL $365/0.1 mL $525/0.2 mL $1,015/2x0.2 mL $1,485/3x0.2 mL $1,930/4x0.2 mL $2,125/5x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Stomach Tissue))

IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Defensin Beta 1 (DEFb1), Polyclonal Antibody (Cat# AAA132022)

Full Name

Polyclonal Antibody to Defensin Beta 1 (DEFb1)

Gene Names

Defb1; CDK4

Reactivity

Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $555/1 mL $1,495/5 mL $2,915/2x5 mL $4,260/3x5 mL $5,530/4x5 mL $6,760/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Lymph node lysate; Primary Ab: 5ug/ml Rabbit Anti-Human Lnk Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Lymphocyte Adaptor Protein (Lnk), Polyclonal Antibody (Cat# AAA135342)

Full Name

Polyclonal Antibody to Lymphocyte Adaptor Protein (Lnk)

Gene Names

SH2B3; LNK; IDDM20

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ITSN2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using ITSN2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ITSN2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using ITSN2 Polyclonal Antibody at dilution of 1:1000.)

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ITSN2, Polyclonal Antibody (Cat# AAA177390)

Full Name

ITSN2 Polyclonal Antibody

Gene Names

ITSN2; SWA; SWAP; SH3D1B; SH3P18; PRO2015

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using GFPT1 antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using GFPT1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GFPT1 antibody.)

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GFPT1, Polyclonal Antibody (Cat# AAA174122)

Full Name

GFPT1 Polyclonal Antibody

Gene Names

GFPT1; GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ALKBH8 Polyclonal Antibody at dilution of 1:25(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ALKBH8 Polyclonal Antibody at dilution of 1:25(×200))

WB (Western Blot) (Western blot analysis of A549 and Jurkat cell lysates using ALKBH8 Polyclonal Antibody at dilution of 1:300)

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ALKBH8, Polyclonal Antibody (Cat# AAA176225)

Full Name

ALKBH8 Polyclonal Antibody

Gene Names

ALKBH8; ABH8; TRM9; MRT71; TRMT9; TRMT9A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using IRF5 Polyclonal Antibody at dilution of 1:25(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using IRF5 Polyclonal Antibody at dilution of 1:25(×200))

WB (Western Blot) (Western blot analysis of NIH/3T3 Hela Jurkat Hepg2 and A549 cell lysates using IRF5 Polyclonal Antibody at dilution of 1:250)

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IRF5, Polyclonal Antibody (Cat# AAA175794)

Full Name

IRF5 Polyclonal Antibody

Gene Names

IRF5; SLEB10

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using RELN Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells using RELN Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using RELN Polyclonal Antibody at 1:1000 dilution.)

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RELN, Polyclonal Antibody (Cat# AAA179528)

Full Name

RELN Polyclonal Antibody

Gene Names

RELN; RL; ETL7; LIS2; PRO1598

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse eye using SYT1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat eye using SYT1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using SYT1 Polyclonal Antibody at dilution of 1:200 (40x lens).)

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SYT1, Polyclonal Antibody (Cat# AAA177082)

Full Name

SYT1 Polyclonal Antibody

Gene Names

SYT1; P65; SYT; SVP65

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using MAD2L1BP Polyclonal Antibody at dilution 1:60)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer tissue using MAD2L1BP Polyclonal Antibody at dilution 1:60)

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MAD2L1BP, Polyclonal Antibody (Cat# AAA167302)

Full Name

MAD2L1BP Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human stomach tissue using Akt1 Polyclonal Antibody at dilution of 1:200.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus tissue using Akt1 Polyclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of various cells using Akt1 Polyclonal Antibody at dilution of 1:2000.)

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Akt1, Polyclonal Antibody (Cat# AAA172062)

Full Name

Akt1 Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human brain using AHSG Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using AHSG Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of Human plasma tissue using AHSG Polyclonal Antibody at dilution of 1:1350)

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AHSG, Polyclonal Antibody (Cat# AAA169960)

Full Name

AHSG Polyclonal Antibody

Gene Names

AHSG; AHS; A2HS; HSGA; FETUA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western blot of HOXA9 Antibody (C-term) in A2058 cell line lysates (35 ug/lane). HOXA9 (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry) (HOXA9 Antibody (C-term) flow cytometry of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Anti-HOXA9 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

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HOXA9, Polyclonal Antibody (Cat# AAA52410)

Full Name

Rabbit Polyclonal to Human HOXA9

Gene Names

HOXA9; HOX1; ABD-B; HOX1G; HOX1.7

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Ammonium sulfate precipitation

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (Western blot of extracts from HepG2 cells, using LEG9 Antibody. The lane on the right is treated with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence of NIH-3T3 cells, using LEG9 Antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Anti-LGALS9 / Galectin-9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

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LGALS9 / Galectin 9, Polyclonal Antibody (Cat# AAA52059)

Full Name

Rabbit Polyclonal (IgG) to Human LGALS9 / Galectin 9

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BTK antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using BTK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using BTK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using BTK antibody at dilution of 1:100 (40x lens).)

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BTK, Polyclonal Antibody (Cat# AAA37198)

Full Name

BTK Polyclonal Antibody

Gene Names

BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.)

IHC (Immunohiostchemistry) (Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.)

WB (Western Blot) (Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.)

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DTX4, Polyclonal Antibody (Cat# AAA40629)

Full Name

DTX4 Antibody

Gene Names

DTX4; RNF155

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

DTX4 antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of JK cells using anti-NFIA antibody (AAA127185).Overlay histogram showing JK cells stained with AAA127185 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (AAA127185, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of NFIA using anti-NFIA antibody (AAA127185).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat RH35 whole cell lysates,Lane 6: mouse L929 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (#AAA127185) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFIA at approximately 70 kDa. The expected band size for NFIA is at 56 kDa.)

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NFIA, Polyclonal Antibody (Cat# AAA127185)

Full Name

Anti-NFIA Antibody Picoband

Gene Names

NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-ATL1 antibody (AAA127091).Overlay histogram showing SH-SY5Y cells stained with AAA127091 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ATL1 Antibody (AAA127091, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of ATL1 using anti-ATL1 antibody (AAA127091).ATL1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-ATL1 Antibody (AAA127091) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of ATL1 using anti-ATL1 antibody (AAA127091).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human SiHa whole cell lysates,Lane 3: human SH-SY5Y whole cell lysates,Lane 4: human U20S whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATL1 antigen affinity purified polyclonal antibody (#AAA127091) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATL1 at approximately 64 kDa. The expected band size for ATL1 is at 64 kDa.)

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ATL1, Polyclonal Antibody (Cat# AAA127091)

Full Name

Anti-ATL1 Antibody Picoband

Gene Names

ATL1; FSP1; GBP3; SPG3; HSN1D; SPG3A; AD-FSP; atlastin1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SEC61B antibody (AAA127438).Overlay histogram showing PC-3 cells stained with AAA127438 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC61B Antibody (AAA127438, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of SEC61B using anti-SEC61B antibody (AAA127438).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse C2C12 whole cell lysates,Lane 7: mouse RAW264.7 whole cell lysates,Lane 8: mouse 3T3-L1 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61B antigen affinity purified polyclonal antibody (#AAA127438) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SEC61B at approximately 15 kDa. The expected band size for SEC61B is at 10 kDa.)

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SEC61B, Polyclonal Antibody (Cat# AAA127438)

Full Name

Anti-SEC61B Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

WB (Western Blot) (Anti-IL-6 antibody, AAA45128, Western blottingLane 1: Recombinant Mouse IL-4 Protein 10ngLane 2: Recombinant Mouse IL-4 Protein 5ng)

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IL-6, Polyclonal Antibody (Cat# AAA45128)

Full Name

Anti-IL-6 antibody

Gene Names

Il6; ILg6; Ifnb2

Reactivity

Mouse, Rat

Applications

Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) (Anti-C5/C5a antibody, AAA45692, All Western blottingAll lanes: Anti-C5a(AAA45692) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 40ugLane 2: Mouse Liver Tissue Lysate at 40ugLane 3: NIH Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: PC12 Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 115KD)

IHC (Immunohistochemisry) (Anti-C5/C5a antibody, AAA45692, IHC(P)IHC(P): Rat Spleen Tissue)

IHC (Immunohiostchemistry) (Anti-C5/C5a antibody, AAA45692, IHC(P)IHC(P): Rat Liver Tissue)

IHC (Immunohistochemistry) (Anti-C5/C5a antibody, AAA45692, IHC(P)IHC(P): Mouse Spleen Tissue)

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C5/C5a, Polyclonal Antibody (Cat# AAA45692)

Full Name

Anti-C5/C5a antibody

Gene Names

C5; Hc; C5a; RGD1561905

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemisry) (Anti- SIRT7 Picoband antibody, AAA46507, IHC(P)IHC(P): Rat Spleen Tissue)

IHC (Immunohiostchemistry) (Anti- SIRT7 Picoband antibody, AAA46507, IHC(P)IHC(P): Mouse Spleen Tissue)

WB (Western Blot) (Anti- SIRT7 Picoband antibody, AAA46507, Western blottingAll lanes: Anti SIRT7 (AAA46507) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)

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SIRT7, Polyclonal Antibody (Cat# AAA46507)

Full Name

Anti-SIRT7 Antibody

Gene Names

SIRT7; SIR2L7

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (DHODH was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- DHODH Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemisry) (DHODH was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- DHODH Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry) (DHODH was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- DHODH Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of DHODH expression in rat liver extract (lane 1), mouse spleen extract (lane 2) and HEPG2 whole cell lysates (lane 3). DHODH at 43KD was detected using rabbit anti- DHODH Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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DHODH, Polyclonal Antibody (Cat# AAA46830)

Full Name

Anti-DHODH Antibody

Gene Names

DHODH; URA1; POADS; DHOdehase

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Figure 5. IF analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (AAA126213).DEP1/PTPRJ was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-DEP1/PTPRJ Antibody (AAA126213) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HEL cells using anti-DEP1/PTPRJ antibody (AAA126213).Overlay histogram showing HEL cells stained with AAA126213 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DEP1/PTPRJ Antibody (AAA126213, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (AAA126213).DEP1/PTPRJ was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-DEP1/PTPRJ Antibody (AAA126213) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (AAA126213).DEP1/PTPRJ was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-DEP1/PTPRJ Antibody (AAA126213) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (AAA126213).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEP1/PTPRJ antigen affinity purified polyclonal antibody (#AAA126213) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DEP1/PTPRJ at approximately 240 kDa. The expected band size for DEP1/PTPRJ is at 146 kDa.)

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DEP1/PTPRJ, Polyclonal Antibody (Cat# AAA126213)

Full Name

Anti-DEP1/PTPRJ Antibody Picoband

Gene Names

PTPRJ; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

WB (Western Blot) ((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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RASSF6, Polyclonal Antibody (Cat# AAA61328)

Full Name

Goat anti-RASSF6 Antibody

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohiostchemistry) ((3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GBX2, Polyclonal Antibody (Cat# AAA61117)

Full Name

Goat anti-GBX2 Antibody

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohistochemistry) ((3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) ((0.1ug/ml) staining of Pig Skeletal Muscle (A) and Heart (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) ((0.3ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) ((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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CAV3, Polyclonal Antibody (Cat# AAA61412)

Full Name

Goat anti-CAV3 Antibody

Gene Names

CAV3; LQT9; VIP21; LGMD1C; VIP-21

Reactivity

Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot)

WB (Western Blot)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer. Magnification: 20x. Slice thickness: 7 microns)

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GRP78 (Bip), Polyclonal Antibody (Cat# AAA103517)

Full Name

GRP78 Antibody: RPE

Gene Names

Hspa5; BIP; GRP78

Reactivity

Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus

Applications

Immunofluorescence, Western Blot

Pricing

$650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,625/5x0.2 mL

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IF (Immunofluorescence)

IF (Immunofluorescence)

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Leptospira Lig A/B, Polyclonal Antibody (Cat# AAA76876)

Full Name

Anti-Canine Leptospira Lig A/B Antibody-Affinity Purified

Reactivity

Canine/Dog

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Antigen Affinity Purified

Pricing

$525/1 mg $1,015/2x1 mg $1,485/3x1 mg $1,930/4x1 mg $2,165/5x1 mg

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: A431 cell, Primary antibody: AAA242033(ABCC1 Antibody) at dilution 1/350 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA242033(ABCC1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA242033(ABCC1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

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ABCC1, Polyclonal Antibody (Cat# AAA242033)

Full Name

ABCC1 Antibody

Gene Names

ABCC1; MRP; ABCC; GS-X; MRP1; ABC29

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Ab-358) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60, MCF and K562 cells using Myc(Ab-358) Antibody.)

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MYC, Polyclonal Antibody (Cat# AAA242073)

Full Name

MYC (Ab-358) Antibody

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using AAA240093(CHRNA10 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA240093(CHRNA10 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CHRNA10, Polyclonal Antibody (Cat# AAA240093)

Full Name

CHRNA10 Antibody

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.