At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using VAMP4 Polyclonal Antibody at dilution of 1:50(×200))
WB (Western Blot) (Western blot analysis of K562 cell and Mouse brain tissue lysates using VAMP4 Polyclonal Antibody at dilution of 1:400)
IF (Immunofluorescence) (Immunofluorescence analysis of human skin cells using FLG Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using FLG Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines using FLG Polyclonal Antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using CCNB1IP1 Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using CCNB1IP1 Polyclonal Antibody at dilution of 1:30(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using ANKS6 Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using ANKS6 Polyclonal Antibody at dilution of 1:55(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human prost ate cancer tissue using ELF5 Polyclonal Antibody at dilution of 1:75(×200))
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using ELF5 Polyclonal Antibody at dilution of 1:75(×200))
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PADI4 Polyclonal Antibody at dilution of 1:35(×200))
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using PADI4 Polyclonal Antibody at dilution of 1:35(×200))
WB (Western Blot) (Western blot analysis of 293T cell lysates using PADI4 Polyclonal Antibody at dilution of 1:400)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Mouse spinal cord using SLC2A13 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat brain using SLC2A13 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using SLC2A13 Polyclonal Antibody at dilution of 1:100 (40x lens).)
IF (Immunofluorescence) (AAA324076 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohistochemisry) (AAA324076 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using TAF15 antibody.)
WB (Western Blot) (Western blot analysis of extracts from 293 and rat spleen, using TAF15 Antibody.)
IHC (Immunohistochemisry) (AAA324911 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA324911 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts from A549 cells, using NUMA1 antibody.)
IF (Immunofluorescence) (AAA322279 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of eNOS expression in Insulin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (Fig: 3 Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using at 1: 2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.)
IHC (Immunohiostchemistry) (Fig: 2 Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using at 1: 2000. Hematoxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow.)
WB (Western Blot) (Fig: 1 Western blot analysis of TRAF6 in normal human tissues using at 1: 2000. TRAF6 is observed at ~66 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments.)
IHC (Immunohiostchemistry) ((10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
WB (Western Blot) (Staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using LTA4H antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LTA4H antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LRP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
ICC (Immunocytochemistry) (Immunocytochemistry of IKKa in Jurkat cells with IKK alpha antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of IKKa in Jurkat cells with IKK alpha antibody at 20 μg/mL.Green: IKK alpha Antibody (2025)Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 μg/mL.)
ICC (Immunocytochemistry) (Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1μg/mL.)
WB (Western Blot) (Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1172) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1172) Antibody.)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using AAA242021(ADAMTS1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA242021(ADAMTS1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
WB (Western Blot) (Western blot analysis of extracts from 293 cells (Lane 2) and HepG2 cells (Lane 3), using Keratin 5 antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using Keratin 5 antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 5 antibody.)
WB (Western Blot) (Western blot analysis of extracts from Rat brain tissue or calf intestinal phosphatase (CIP), using Synapsin (phospho-Ser549) Antibody.)
WB (Western Blot) (Western blot analysis of extract from rat brain and mouse brain tissue using Synapsin(phospho-Ser549) Antibody using)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
IHC (Immunohiostchemistry) (IHC image of AAA243551 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of AAA243551 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney using CYCS antibody at dilution of 1:200 (200x lens).)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human thyroid cancer using CYCS antibody at dilution of 1:200 (200x lens).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Colon cancer using CYCS antibody.)
WB (Western Blot) (Western blot analysis of extracts of mouse kidney cell line, using CYCS antibody.)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46503(CISD1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200))
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with Dysferlin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Dysferlin on human fetal skeletal muscle tissue lysate using anti-Dysferlin antibody at 1/500 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of PC-3M cells with IL1 alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL1 alpha antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution.)
IHC (Immunohiostchemistry) (C9orf156 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C9orf156 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of C9orf156 Antibody (C-term) in Hela, CEM, T47D cell line lysates (35ug/lane). C9orf156 (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (GSTA4 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human brain tissue reacted with GSTA4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of GSTA4 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). GSTA4 (arrow) was detected using the purified Pab.)
WB (Western Blot) (Mouse Cdk15 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Cdk15 antibody detected the Cdk15 protein (arrow).)
WB (Western Blot) (Mouse Cdk15 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Cdk15 antibody detected the Cdk15 protein (arrow).)
FCM/FACS (Flow Cytometry) (PRUNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (PRUNE Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of PRUNE Antibody (C-term) in Jurkat cell line lysates (35ug/lane). PRUNE (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry) (CCR1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry) (CCR1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CCR1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot) (Western blot analysis of CCR1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CCR1 (arrow) was detected using the purified Pab.)
WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using RASH/RASK Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RASH/RASK Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Detection of human CSN1 by western blot. Samples: Whole cell lysate (50 ug-E; 10 ug-T) from mock transfected (E) or CSN1 transfected (T) HEKHEK293T cells. Antibody: Affinity purified rabbit anti-CSN1 AAA210725 used at the indicated concentration. Detection: Chemiluminescence with a 10 second exposure.)
WB (Western Blot) (Detection of human CSN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody AAA210725 (lot AAA210725-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry) (Detection of human CSN1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSN1 (Cat. No. AAA210725) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
WB (Western Blot) (Detection of human BAG3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAG3 antibody AAA212023 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. BAG3 was also immunoprecipitated by rabbit anti-BAG3 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry) (Detection of human BAG3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BAG3 (Cat. No. AAA212023) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
WB (Western Blot) (Detection of human Nucleobindin 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation) (Detection of human Nucleobindin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nucleobindin 1 antibody AAA212967 (lot AAA212967-1) used for IP at 6 ug per reaction. Nucleobindin 1 was also immunoprecipitated by rabbit anti-Nucleobindin 1 antibody A304-332A. For blotting immunoprecipitated Nucleobindin 1, AAA212967 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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