Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using ADCK4 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using ADCK4 antibody.)

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ADCK4, Polyclonal Antibody (Cat# AAA242471)

Full Name

ADCK4 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HuvEc cells, using RPS4Y1 antibody.)

WB (Western Blot) (Western blot analysis of extracts from COLO cells, HeLa cells and HUVEC cells, using RPS4Y1 antibody.)

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RPS4Y1, Polyclonal Antibody (Cat# AAA242557)

Full Name

RPS4Y1 Antibody

Gene Names

RPS4Y1; S4; RPS4Y

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: AAA240908(CTSC Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using AAA240908(CTSC Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CTSC, Polyclonal Antibody (Cat# AAA240908)

Full Name

CTSC Antibody

Gene Names

CTSC; JP; HMS; JPD; PLS; CPPI; DPP1; DPPI; PALS; DPP-I; PDON1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA240395(CR2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using AAA240395(CR2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CR2, Polyclonal Antibody (Cat# AAA240395)

Full Name

CR2 Antibody

Gene Names

CR2; CR; C3DR; CD21; CVID7; SLEB9

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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SDS-PAGE (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Jurkat, A549, hepg2 and NIH/3T3 cell, Primary antibody: AAA241968(PKM Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA241968(PKM Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using AAA241968(PKM Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

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PKM, Polyclonal Antibody (Cat# AAA241968)

Full Name

PKM Antibody

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr69 or 51) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr69 or 51) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2(Phospho-Thr69 or 51) Antibody.)

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ATF2, Polyclonal Antibody (Cat# AAA243261)

Full Name

Phospho-ATF2 (Thr69 or 51) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of SiHa cells using anti-IFI44L antibody (AAA128225).Overlay histogram showing SiHa cells stained with AAA128225 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFI44L Antibody (AAA128225, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of IFI44L using anti-IFI44L antibody (AAA128225).IFI44L was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4ug/mL rabbit anti-IFI44L Antibody (AAA128225) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of IFI44L using anti-IFI44L antibody (AAA128225).IFI44L was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFI44L Antibody (AAA128225) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of IFI44L using anti-IFI44L antibody (AAA128225).IFI44L was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFI44L Antibody (AAA128225) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of IFI44L using anti-IFI44L antibody (AAA128225).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SiHa whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: mouse NRK whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFI44L antigen affinity purified polyclonal antibody (AAA128225) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFI44L at approximately 51 kDa. The expected band size for IFI44L is at 51 kDa.)

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IFI44L, Polyclonal Antibody (Cat# AAA128225)

Full Name

Anti-IFI44L Antibody Picoband

Gene Names

IFI44L; GS3686; C1orf29

Reactivity

Human, Monkey, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Cubilin (CUBN), Polyclonal Antibody (Cat# AAA132951)

Full Name

Polyclonal Antibody to Cubilin (CUBN)

Gene Names

Cubn; AA408369; AL022750; D2Wsu88e

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P;Samples: Human Prostate Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human PLAA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin- embedded prostate gland tissue)

WB (Western Blot) (Figure. Western blot; Sample: Recombinant PLAP, Human.)

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Phospholipase A2 Activating Protein (PLAA), Polyclonal Antibody (Cat# AAA133286)

Full Name

Polyclonal Antibody to Phospholipase A2 Activating Protein (PLAA)

Gene Names

PLAA; DOA1; PLAP; PLA2P; NDMSBA

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$210/0.1 mL $255/0.2 mL $410/0.5 mL $490/1 mL $1,320/5 mL $2,565/2x5 mL $3,740/3x5 mL $4,860/4x5 mL $5,940/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Brain Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: HumanStomach Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant SEPT5, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Porcine Heart Tissue; Lane3: Human HepG2 Cells.)

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Septin 5 (SEPT5), Polyclonal Antibody (Cat# AAA133347)

Full Name

Polyclonal Antibody to Septin 5 (SEPT5)

Gene Names

SEPT5; H5; CDCREL; PNUTL1; CDCREL1; CDCREL-1; HCDCREL-1

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Platelet Derived Growth Factor AA (PDGFAA), Polyclonal Antibody (Cat# AAA135307)

Full Name

Polyclonal Antibody to Platelet Derived Growth Factor AA (PDGFAA)

Reactivity

Mouse

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$210/0.1 mL $265/0.2 mL $415/0.5 mL $500/1 mL $1,360/5 mL $2,645/2x5 mL $3,860/3x5 mL $5,010/4x5 mL $6,080/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:RatTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Protein Disulfide Isomerase (PDI), Polyclonal Antibody (Cat# AAA131932)

Full Name

Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

Gene Names

P4hb; PDI; PDIR

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $555/1 mL $1,495/5 mL $2,915/2x5 mL $4,260/3x5 mL $5,530/4x5 mL $6,760/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:RatTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Apelin (APLN), Polyclonal Antibody (Cat# AAA132051)

Full Name

Polyclonal Antibody to Apelin (APLN)

Gene Names

Apln; Apel

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $290/0.2 mL $480/0.5 mL $585/1 mL $1,575/5 mL $3,055/2x5 mL $4,470/3x5 mL $5,795/4x5 mL $7,085/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant RI, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Mouse Heart Tissue.)

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Ribonuclease Inhibitor (RI), Polyclonal Antibody (Cat# AAA135339)

Full Name

Polyclonal Antibody to Ribonuclease Inhibitor (RI)

Gene Names

RNH1; RAI; RNH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $265/0.2 mL $420/0.5 mL $510/1 mL $1,385/5 mL $2,695/2x5 mL $3,935/3x5 mL $5,110/4x5 mL $6,225/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CES2 Polyclonal Antibody at dilution of 1:55(×200))

WB (Western Blot) (Western blot analysis of Human fetal liver tissue lysate using CES2 Polyclonal Antibody at dilution of 1:600)

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CES2, Polyclonal Antibody (Cat# AAA175908)

Full Name

CES2 Polyclonal Antibody

Gene Names

CES2; iCE; CE-2; PCE-2; CES2A1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer using E2F6 Polyclonal Antibody at dilution of 1:60)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer using E2F6 Polyclonal Antibody at dilution of 1:60)

WB (Western Blot) (Western Blot analysis of LoVo and A549 cell, Human hepatocellular carcinoma tissue, Jurkat and Hela cell using E2F6 Polyclonal Antibody at dilution of 1:650)

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E2F6, Polyclonal Antibody (Cat# AAA168736)

Full Name

E2F6 Polyclonal Antibody

Gene Names

E2F6; E2F-6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AOAH Polyclonal Antibody at dilution of 1:25(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AOAH Polyclonal Antibody at dilution of 1:25(×200))

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AOAH, Polyclonal Antibody (Cat# AAA176419)

Full Name

AOAH Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using KRT6A/KRT6B/KRT6C Polyclonal Antibody at dilution 1:40)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using KRT6A/KRT6B/KRT6C Polyclonal Antibody at dilution 1:40)

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KRT6A/KRT6B/KRT6C, Polyclonal Antibody (Cat# AAA168313)

Full Name

KRT6A/KRT6B/KRT6C Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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Application Data (Immunohistochemical staining of human liver with Rabbit anti Human GAPDH (AAA50052))

Application Data (Western blot analysis of whole cell lysate from HeLa, human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH (AAA50052) at 0.125(A), 0.25(B) and 0.5(C) ug/ml)

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GAPDH, Polyclonal Antibody (Cat# AAA50052)

Full Name

RABBIT ANTI GAPDH (N-TERMINAL)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$560/0.1 mg $1,080/2x0.1 mg $1,580/3x0.1 mg $2,050/4x0.1 mg $2,265/5x0.1 mg

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FCM/FACS (Flow Cytometry) (LTF Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot of LTF Antibody in MDA-MB231 cell line lysates (35 ug/lane). LTF (arrow) was detected using the purified antibody.)

WB (Western Blot) (Western blot of LTF Antibody in mouse spleen tissue lysates (35 ug/lane). LTF (arrow) was detected using the purified antibody.)

IHC (Immunohistochemistry) (Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

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LF / LTF / Lactoferrin, Polyclonal Antibody (Cat# AAA52632)

Full Name

Anti-LF / LTF / Lactoferrin Antibody IHC-plus

Gene Names

LTF; LF; HLF2; GIG12; HEL110

Reactivity

Mouse, Human

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (Western blot of DARC in mouse brain tissue lysate with DARC antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

IHC (Immunohiostchemistry) (Anti-DARC antibody IHC of human colon, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-DARC antibody IHC of human spleen, red pulp. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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DARC, Polyclonal Antibody (Cat# AAA50863)

Full Name

Rabbit Polyclonal to Human DARC

Gene Names

ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$765/0.05 mg $1,495/2x0.05 mg $2,185/3x0.05 mg $2,835/4x0.05 mg $3,235/5x0.05 mg

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WB (Western Blot) (Antibody (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-Pyruvate Carboxylase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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PC / Pyruvate Carboxylase, Polyclonal Antibody (Cat# AAA50882)

Full Name

Goat Polyclonal to Human PC / Pyruvate Carboxylase

Gene Names

PC; PCB

Reactivity

Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Goat (at least 90% immunogen sequence identity)

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

WB (Western Blot) ((1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GLuR5/GRIK1, Polyclonal Antibody (Cat# AAA60891)

Full Name

Goat anti-GLuR5/GRIK1 Antibody

Gene Names

GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1; gluR-5

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

WB (Western Blot) ((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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TCF2/VHNF1, Polyclonal Antibody (Cat# AAA61008)

Full Name

Goat anti-TCF2/VHNF1 Antibody

Gene Names

HNF1B; FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta; HNF-1-beta

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

Applications

Immunocytochemistry, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IF (Immunofluorescence) (Staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).)

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)

WB (Western Blot) ((1ug/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1ug/ml) negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

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FOXP3/SCURFIN, Polyclonal Antibody (Cat# AAA61014)

Full Name

Goat anti-FOXP3/SCURFIN Antibody

Gene Names

FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Immunocytochemistry, Flow Cytometry, Immunofluorescence, Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) ( )

IHC (Immunohistochemisry) (Anti-TCF7L2 antibody IHC of human small intestine.)

IHC (Immunohiostchemistry) (Anti-TCF7L2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-TCF7L2 antibody IHC of human placenta.)

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TCF7L2 / TCG4, Polyclonal Antibody (Cat# AAA51755)

Full Name

Rabbit Polyclonal (IgG) to Human TCF7L2 / TCG4

Gene Names

TCF7L2; TCF4; TCF-4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohiostchemistry) (AAA61652 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

WB (Western Blot) (AAA61652 (0.1ug/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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Alpha-synuclein, Polyclonal Antibody (Cat# AAA61652)

Full Name

Goat anti-Alpha-synuclein Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) ((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) ((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GLP-1-R, Polyclonal Antibody (Cat# AAA61687)

Full Name

Goat anti-GLP-1-R (mouse) Antibody

Gene Names

Glp1r; GLP-1R; GLP1Rc

Reactivity

Tested: Mouse; Expected from sequence similarity: Mouse

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using ATG7 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using ATG7 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using ATG7 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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ATG7, Polyclonal Antibody (Cat# AAA37292)

Full Name

ATG7 Polyclonal Antibody

Gene Names

ATG7; GSA7; APG7L; APG7-LIKE

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of PTPN5 in mouse brain tissue with PTPN5 antibody at 20 μg/ml.)

IHC (Immunohiostchemistry) (Immunohistochemistry of PTPN5 in mouse brain tissue with PTPN5 antibody at 5 μg/ml.)

WB (Western Blot) (Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.)

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PTPN5, Polyclonal Antibody (Cat# AAA40537)

Full Name

PTPN5 Antibody

Gene Names

PTPN5; STEP; PTPSTEP

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

PTPN5 antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti-Cystathionase antibody, AAA45171, IHC(P)IHC(P): Human Liver Tissue)

WB (Western Blot) (Anti-Cystathionase antibody, AAA45171, Western blottingLane 1: SMMC Cell LysateLane 2: HT180 Cell LysateLane 3: HELA Cell LysateLane 4: U87 Cell Lysate)

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Cystathionase, Polyclonal Antibody (Cat# AAA45171)

Full Name

Anti-Cystathionase antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (PSAT1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemisry) (PSAT1 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry) (PSAT1 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of PSAT1 expression in rat pancreas extract (lane 1) and HELA whole cell lysates (lane 2). PSAT1 at 40KD was detected using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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PSAT1, Polyclonal Antibody (Cat# AAA46843)

Full Name

Anti-PSAT1 Antibody

Gene Names

PSAT1; PSA; EPIP; NLS2; PSAT; PSATD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 10 % SDS PAGE gel and IKB alpha antibody at a dilution of 1:1000)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1:200 dilution.)

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IKB alpha, Polyclonal Antibody (Cat# AAA107608)

Full Name

IKB alpha antibody

Applications

Western Blot, Immunocytochemistry, Immunofluorescence

Purity

IKB alpha antibody was purified by antigen-affinity chromatography

Pricing

$1,375/0.1 mL $2,675/2x0.1 mL $3,905/3x0.1 mL $5,065/4x0.1 mL $5,950/5x0.1 mL

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FCM/FACS (Flow Cytometry) (METTL10 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (METTL10 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Anti-METTL10 Antibody (N-term) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST..)

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METTL10, Polyclonal Antibody (Cat# AAA283955)

Full Name

METTL10 Antibody (N-term)

Gene Names

METTL10; C10orf138

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (PSAPL1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (PSAPL1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PSAPL1 antibody detected the PSAPL1 protein (arrow).)

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PSAPL1, Polyclonal Antibody (Cat# AAA283857)

Full Name

PSAPL1 Antibody (N-term)

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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SDS-PAGE (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse heart tissue, Primary antibody: AAA240953(CDK19 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds)

IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AAA240953(CDK19 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA240953(CDK19 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

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CDK19, Polyclonal Antibody (Cat# AAA240953)

Full Name

CDK19 Antibody

Gene Names

CDK19; CDK11; CDC2L6; bA346C16.3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (AAA324634 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA324634) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using C1QBP antibody.)

IHC (Immunohiostchemistry) (AAA324634 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using C1QBP antibody.)

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C1QBP, Polyclonal Antibody (Cat# AAA324634)

Full Name

C1QBP Antibody

Gene Names

C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33; SF2AP32

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNAJC7 antibody at 1: 200 dilution.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNAJC7 antibody at 1: 100 dilution.)

Application Data (Sample(30 ug of whole cell lysate)A: 293T B: A4317.5% SDS PAGEPrimary antibody diluted at 1: 1500)

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DNAJC7, Polyclonal Antibody (Cat# AAA306172)

Full Name

DNAJC7 antibody

Gene Names

DNAJC7; DJ11; DJC7; TPR2; TTC2

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by antigen-affinity chromatography.

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using THOC5 antibody.)

WB (Western Blot) (Western blot analysis of extracts from JK cells and HepG2 cells, using THOC5 antiobdy.)

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THOC5, Polyclonal Antibody (Cat# AAA306685)

Full Name

THOC5 Antibody

Gene Names

THOC5; Fmip; PK1.3; fSAP79; C22orf19

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 ug/mL.)

WB (Western Blot) (Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.)

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DRAM, Polyclonal Antibody (Cat# AAA307650)

Full Name

DRAM Antibody

Gene Names

DRAM1; DRAM

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IP (Immunoprecipitation) (IMPA1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-IMPA1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IMPA1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size :30 kDa)

IHC (Immunohiostchemistry) (Immunochemical staining of human IMPA1 in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-IMPA1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)

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IMPA1, Polyclonal Antibody (Cat# AAA256117)

Full Name

Anti-IMPA1 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embeded human colorectal carcinoma using at dilution of 1:50.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embeded human placenta using at dilution of 1:50.)

WB (Western Blot) (All lanes: Prootein FAM110B antibody at 2ug/ml)

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Protein FAM110B, Polyclonal Antibody (Cat# AAA308714)

Full Name

Protein FAM110B Polyclonal Antibody

Gene Names

FAM110B; C8orf72

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$385/0.1 mg $745/2x0.1 mg $1,090/3x0.1 mg $1,410/4x0.1 mg $1,525/5x0.1 mg

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200)

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Imp3, Polyclonal Antibody (Cat# AAA309627)

Full Name

Imp3 Polyclonal Antibody

Gene Names

IMP3; BRMS2; MRPS4; C15orf12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Rat Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Rat CPR Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Cytochrome P450, Polyclonal Antibody (Cat# AAA142022)

Full Name

Polyclonal Antibody to Cytochrome P450 Reductase (CPR)

Gene Names

Por; CYPOR

Reactivity

Rat, Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $290/0.2 mL $480/0.5 mL $585/1 mL $1,575/5 mL $3,055/2x5 mL $4,470/3x5 mL $5,795/4x5 mL $7,085/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:HumanTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Gigaxonin, Polyclonal Antibody (Cat# AAA142634)

Full Name

Polyclonal Antibody to Gigaxonin (GAN)

Gene Names

GAN; GAN1; KLHL16

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) (IHC image diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

IHC (Immunohistochemistry) (IHC image diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

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CA9, Polyclonal Antibody (Cat# AAA243784)

Full Name

CA9 Antibody

Gene Names

CA9; MN; CAIX

Reactivity

Human

Applications

Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IF (Immunofluorescence) (AAA323855 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using CORO1A antibody.The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA323855 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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CORO1A, Polyclonal Antibody (Cat# AAA323855)

Full Name

CORO1A Antibody

Gene Names

CORO1A; p57; IMD8; TACO; CLABP; HCORO1; CLIPINA

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.