Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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WB (Western Blot) (Western blot analysis of extracts from Colo cells, HepG2 cells and 293 cells using AOS1 antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, using AOS1 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.)

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SAE1, Polyclonal Antibody (Cat# AAA242897)

Full Name

SAE1 Antibody

Gene Names

SAE1; AOS1; SUA1; UBLE1A; HSPC140

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CHSY1 antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells and K562 cells, using CHSY1 antibody.)

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CHSY1, Polyclonal Antibody (Cat# AAA242609)

Full Name

CHSY1 Antibody

Gene Names

CHSY1; CHSY; CSS1; TPBS; ChSy-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105(phospho-Ser907) antibody.)

WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with TNF-α using NF-kappa;B p105(phospho-Ser907) antibody.)

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NFKB1, Polyclonal Antibody (Cat# AAA243174)

Full Name

Phospho-NFKB1 (Ser907) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using SAPK/JNK Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SAPK/JNK Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using SAPK/JNK Antibody. The picture on the right is blocked with the synthesized peptide.)

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SAPK/JNK, Polyclonal Antibody (Cat# AAA317082)

Full Name

SAPK/JNK Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29, 293, and HeLa cells, using HTR2B Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using HTR2B Antibody. The picture on the right is blocked with the synthesized peptide.)

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HTR2B, Polyclonal Antibody (Cat# AAA318018)

Full Name

HTR2B Antibody

Gene Names

HTR2B; 5-HT2B; 5-HT-2B; 5-HT(2B)

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HEPG2 823 293T AD293 HELA using SR-1D antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)

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SR-1D, Polyclonal Antibody (Cat# AAA318663)

Full Name

SR-1D Rabbit Polyclonal Antibody

Gene Names

HTR1D; HTRL; RDC4; HT1DA; 5-HT1D; HTR1DA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from K562 cells, using GPR119 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using GPR119 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GPR119, Polyclonal Antibody (Cat# AAA318061)

Full Name

GPR119 Antibody

Gene Names

GPR119; GPCR2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, using EDG4 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using EDG4 Antibody. The picture on the right is blocked with the synthesized peptide.)

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EDG4, Polyclonal Antibody (Cat# AAA318082)

Full Name

EDG4 Antibody

Gene Names

LPAR2; EDG4; LPA2; EDG-4; LPA-2

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Catenin-beta (Phospho-Tyr489) Antibody)

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Catenin-beta, Polyclonal Antibody (Cat# AAA318295)

Full Name

Catenin-beta (Phospho-Tyr489) Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using OR4F4 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using OR4F4 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR4F4, Polyclonal Antibody (Cat# AAA318197)

Full Name

OR4F4 Antibody

Gene Names

OR4F4; OR4F18; OLA-7501

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cell using NAMPT antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer using NAMPT antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded rat spleen using NAMPT antibody at dilution of 1:200 (200x lens).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human ovary cancer using NAMPT antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NAMPT antibody.)

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NAMPT, Polyclonal Antibody (Cat# AAA303851)

Full Name

NAMPT Antibody

Gene Names

NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$335/0.05 mL $425/0.1 mL $830/2x0.1 mL $1,205/3x0.1 mL $1,565/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin b3(Phospho-Tyr773) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells using Integrin b3(Phospho-Tyr773) Antibody and the same antibody preincubated with blocking peptide.)

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Integrin beta3, Polyclonal Antibody (Cat# AAA308011)

Full Name

Integrin beta3 (Phospho-Tyr773) Antibody

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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FCM/FACS (Flow Cytometry) (OC90 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (OC90 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the OC90 antibody detected the OC90 protein (arrow).)

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OC90, Polyclonal Antibody (Cat# AAA289526)

Full Name

OC90 Antibody (Center)

Gene Names

OC90; PLA2L

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).)

WB (Western Blot) (The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3) or DYKDDDDK-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)

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Aurora-C, Polyclonal Antibody (Cat# AAA289195)

Full Name

Aurora-C Antibody (N-term G11)

Gene Names

AURKC; AIE2; AIK3; ARK3; AurC; SPGF5; STK13; HEL-S-90; aurora-C

Reactivity

Human

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma using at dilution of 1:100.)

WB (Western Blot) (All lanes: Glutamate carboxypeptidase 2 antibody at 2ug/ml)

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Glutamate carboxypeptidase 2, Polyclonal Antibody (Cat# AAA309045)

Full Name

Glutamate carboxypeptidase 2 Polyclonal Antibody

Gene Names

FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$385/0.1 mg $745/2x0.1 mg $1,090/3x0.1 mg $1,410/4x0.1 mg $1,525/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of hela cells using EIF3H Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EIF3H Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (EIF3H Antibody (N-term) western blot analysis in Hela cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF3H antibody detected the EIF3H protein (arrow).)

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EIF3H, Polyclonal Antibody (Cat# AAA286544)

Full Name

EIF3H Antibody (C-term)

Gene Names

EIF3H; EIF3S3; eIF3-p40; eIF3-gamma

Reactivity

Human, mouse (Predicted Reactivity: Bovine, Chicken, Xenopus)

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemistry) (CD47 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry) (CD47 Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohiostchemistry) (CD47 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD47 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

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CD47, Polyclonal Antibody (Cat# AAA163139)

Full Name

CD47 Rabbit anti-Human Polyclonal Antibody

Gene Names

CD47; IAP; OA3; MER6

Reactivity

Human

Applications

Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using HMMR Antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA326746 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA326746 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HMMR, Polyclonal Antibody (Cat# AAA326746)

Full Name

HMMR Antibody

Gene Names

HMMR; CD168; IHABP; RHAMM

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA327114 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Hela cell lysate, using HPX Antibody. The lane on the left is treated with the antigen-specific peptide.)

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HPX, Polyclonal Antibody (Cat# AAA327114)

Full Name

HPX Antibody

Gene Names

HPX; HX

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA326687 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of HeLa using Histone H3 antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326687 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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Histone H3, Polyclonal Antibody (Cat# AAA326687)

Full Name

Histone H3 Antibody

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA324076 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemisry) (AAA324076 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using TAF15 antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 and rat spleen, using TAF15 Antibody.)

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TAF15, Polyclonal Antibody (Cat# AAA324076)

Full Name

TAF15 Antibody

Gene Names

TAF15; Npl3; RBP56; TAF2N; TAFII68

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from mouse muscle, mouse spleen, using HMGCR Antibody.)

WB (Western Blot) (Western blot analysis of extracts of hela, using HMGCR antibody. The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA326285 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HMGCR, Polyclonal Antibody (Cat# AAA326285)

Full Name

HMGCR Antibody

Gene Names

HMGCR; LDLCQ3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA322279 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of eNOS expression in Insulin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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eNOS, Polyclonal Antibody (Cat# AAA322279)

Full Name

eNOS Antibody

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA324911 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA324911 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from A549 cells, using NUMA1 antibody.)

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NUMA1, Polyclonal Antibody (Cat# AAA324911)

Full Name

NUMA1 Antibody

Gene Names

NUMA1; NUMA; NMP-22

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of KLHL1 in rat brain tissue with KLHL1 antibody at 10 ug/mL.)

WB (Western Blot) (Western blot analysis of KLHL1 in human brain cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug /ml.)

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KLHL1, Polyclonal Antibody (Cat# AAA307183)

Full Name

KLHL1 Antibody

Applications

Immunohistochemistry, Western Blot

Purity

immunoaffinity purified

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extract from MDA-MB-435 cells untreated or treated with TNF-alpha (20ng/ml, 5min) using NF-kappaB p100(phospho-Ser870) antibody ().)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p100(phospho- Ser870) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p100(phospho-Ser870) antibody (, Red).)

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NFkappaB-p100/p52, Polyclonal Antibody (Cat# AAA303198)

Full Name

NFkappaB-p100/p52(Phospho-Ser870) Antibody

Gene Names

NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/30.)

Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): 231 and Jurkat cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)

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PPARA, Polyclonal Antibody (Cat# AAA304663)

Full Name

PPARA Antibody

Gene Names

PPARA; PPAR; NR1C1; hPPAR; PPARalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of LEKTI staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of SCN4B expression in U87MG, Jurkat whole cell lysates.)

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LEKTI, Polyclonal Antibody (Cat# AAA310914)

Full Name

LEKTI Antibody

Gene Names

SPINK5; NS; NETS; LEKTI; LETKI; VAKTI

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity chromatography

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-DAXX-S213 Pab is used in Western blot to detect Phospho-DAXX-S213 in mouse thymus tissue lysate)

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Phospho-DAXX (S213), Polyclonal Antibody (Cat# AAA288614)

Full Name

Phospho-DAXX (S213) Antibody

Gene Names

DAXX; DAP6; EAP1; BING2

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (CYP26A1 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (CYP26A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP26A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of CYP26A1 Antibody (C-term) in mouse NIH-3T3 cell lysates (35ug/lane).CYP26A1 (arrow) was detected using the purified Pab.)

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CYP26A1, Polyclonal Antibody (Cat# AAA288472)

Full Name

CYP26A1 Antibody (C-term)

Gene Names

CYP26A1; CP26; CYP26; P450RAI; P450RAI1

Reactivity

Human, mouse

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Mucin 1 Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 3T3 cells using Mucin 1 Polyclonal Antibody)

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Mucin 1, Polyclonal Antibody (Cat# AAA304759)

Full Name

Mucin 1 Polyclonal Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/30.)

Application Data (Gel: 10%SDS-PAGE Lysates (from left to right): HT-29 and Jurkat cell Amount of lysate: 50ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

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SRF, Polyclonal Antibody (Cat# AAA305903)

Full Name

SRF Antibody

Gene Names

SRF; MCM1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (TRUB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRUB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (TRUB1 Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the TRUB1 antibody detected the TRUB1 protein (arrow).)

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TRUB1, Polyclonal Antibody (Cat# AAA289694)

Full Name

TRUB1 Antibody (Center)

Gene Names

TRUB1; PUS4

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Liver Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human U87MG cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human PTX3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Pentraxin 3, Long (PTX3), Polyclonal Antibody (Cat# AAA132922)

Full Name

Polyclonal Antibody to Pentraxin 3, Long (PTX3)

Gene Names

PTX3; TSG-14; TNFAIP5

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) (DABstainingonIHC-P.Samples:RatTissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Beta-Site APP Cleaving Enzyme 1 (bACE1), Polyclonal Antibody (Cat# AAA132982)

Full Name

Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1)

Gene Names

Bace1; Bace

Reactivity

Rat, Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

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Large Multifunctional Peptidase 7 (LMP7), Polyclonal Antibody (Cat# AAA135448)

Full Name

Polyclonal Antibody to Large Multifunctional Peptidase 7 (LMP7)

Gene Names

Psmb8; Lmp7; Lmp-7

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$215/0.1 mL $280/0.2 mL $435/0.5 mL $525/1 mL $1,420/5 mL $2,765/2x5 mL $4,035/3x5 mL $5,240/4x5 mL $6,405/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human Hela cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human PPL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Periplakin (PPL), Polyclonal Antibody (Cat# AAA130774)

Full Name

Polyclonal Antibody to Periplakin (PPL)

Reactivity

Human, Mouse, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Brain Tissue))

IHC (Immunohistochemisry) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human K562 cell lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse TOP2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Topoisomerase II Beta (TOP2b), Polyclonal Antibody (Cat# AAA135022)

Full Name

Polyclonal Antibody to Topoisomerase II Beta (TOP2b)

Gene Names

Top2b; Top-2; D230016L12Rik

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using COX16 Polyclonal Antibody at dilution 1:50)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using COX16 Polyclonal Antibody at dilution 1:50)

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COX16, Polyclonal Antibody (Cat# AAA168846)

Full Name

COX16 Polyclonal Antibody

Gene Names

COX16; HSPC203; C14orf112

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human 293T cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse PTGS2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Prostaglandin Endoperoxide Synthase 2 (PTGS2), Polyclonal Antibody (Cat# AAA133605)

Full Name

Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2)

Gene Names

Ptgs2; COX2; Cox-2; PES-2; PHS-2; Pghs2; TIS10; PGHS-2; PHS II; gripghs

Reactivity

Mouse, Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Affinity Chromatography

Pricing

$210/0.1 mL $265/0.2 mL $415/0.5 mL $500/1 mL $1,360/5 mL $2,645/2x5 mL $3,860/3x5 mL $5,010/4x5 mL $6,080/5x5 mL

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IHC (Immunohistochemistry) (Anti-MTNR1B / MT2 antibody IHC of human eye. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemisry) (Anti-MTNR1B / MT2 antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry) (Anti-MTNR1B / MT2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-MTNR1B antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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MTNR1B / MT2, Polyclonal Antibody (Cat# AAA52022)

Full Name

Rabbit Polyclonal to Human MTNR1B / MT2

Gene Names

MTNR1B; MT2; FGQTL2; MEL-1B-R

Reactivity

Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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FCM/FACS (Flow Cytometry) (CLDN8 Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot of CLDN8 Antibody in 293 cell line lysates (35 ug/lane). CLDN8 (arrow) was detected using the purified antibody.)

IHC (Immunohistochemistry) (Anti-CLDN8 / Claudin 8 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

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CLDN8 / Claudin 8, Polyclonal Antibody (Cat# AAA52600)

Full Name

Anti-CLDN8 / Claudin 8 Antibody (aa140-166) IHC-plus

Gene Names

CLDN8; HEL-S-79

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Immunoaffinity purified

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast using BRCA2 Polyclonal Antibody at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon using BRCA2 Polyclonal Antibody at dilution of 1:100)

WB (Western Blot) (Western Blot analysis of Rat testis using BRCA2 Polyclonal Antibody at dilution of 1:2000)

WB (Western Blot) (Western Blot analysis of Hela cells using BRCA2 Polyclonal Antibody at dilution of 1:2000)

WB (Western Blot) (Western Blot analysis of Jurkat, MCF-7 and A431 cells using BRCA2 Polyclonal Antibody at dilution of 1:2000)

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BRCA2, Polyclonal Antibody (Cat# AAA174076)

Full Name

BRCA2 Polyclonal Antibody

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$195/0.025 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

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WB (Western Blot) (Anti-DNAPKcs Antibody - Western Blot. Western blot of Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kD band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.)

IHC (Immunohistochemistry) (Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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PRKDC / DNA-PKcs, Polyclonal Antibody (Cat# AAA50737)

Full Name

Rabbit Polyclonal to Human PRKDC / DNA-PKcs

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs

Reactivity

Chimpanzee, Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$1,095/0.05 mg $2,125/2x0.05 mg $3,105/3x0.05 mg $4,025/4x0.05 mg $4,680/5x0.05 mg

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WB (Western Blot) (Western blot of Slitrk2 in rat brain tissue lysate with Slitrk2 antibody at (A) 1 and (B) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of Slitrk2 in Human Brain cells with Slitrk2 antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-SLITRK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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SLITRK2, Polyclonal Antibody (Cat# AAA50985)

Full Name

Rabbit Polyclonal to Human SLITRK2

Gene Names

SLITRK2; CXorf2; SLITL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$765/0.05 mg $1,495/2x0.05 mg $2,185/3x0.05 mg $2,835/4x0.05 mg $3,235/5x0.05 mg

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WB (Western Blot) (Antibody (0.3 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-5HT3A receptor antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

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HTR3A / 5-HT3A Receptor, Polyclonal Antibody (Cat# AAA51062)

Full Name

Goat Polyclonal to Human HTR3A / 5-HT3A Receptor

Gene Names

HTR3A; HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R

Reactivity

Chimpanzee, Gorilla, Human
Predicted Reactivity: Orangutan (at least 90% immunogen sequence identity)

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using FCGR2A antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FCGR2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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FCGR2A, Polyclonal Antibody (Cat# AAA36943)

Full Name

FCGR2A Polyclonal Antibody

Gene Names

FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Anti-Annexin IV antibody, AAA45479, IHC(P)IHC(P): Rat Pancreas Tissue)

IHC (Immunohiostchemistry) (Anti-Annexin IV antibody, AAA45479, IHC(P)IHC(P): Human Mammary Cancer Tissue)

WB (Western Blot) (Anti-Annexin IV antibody, AAA45479, Western blottingLane 1: Human Placenta Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: 293T Cell LysateLane 4: HEPA Cell LysateLane 5: NIH3T3 Cell Lysate)

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Annexin IV, Polyclonal Antibody (Cat# AAA45479)

Full Name

Anti-Annexin IV antibody

Gene Names

ANXA4; ANX4; PIG28; ZAP36; HEL-S-274

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohiostchemistry) (Anti- Oncostatin M Picoband antibody, AAA46343, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot) (Anti- Oncostatin M Picoband antibody, AAA46343, Western blottingAll lanes: Anti Oncostatin M (AAA46343) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HUT Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 40KD)

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Oncostatin M, Polyclonal Antibody (Cat# AAA46343)

Full Name

Anti-Oncostatin M Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.