Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry) (RNF19A Antibody (Center) (AAA288911)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF19A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (RNF19A Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF19A antibody detected the RNF19A protein (arrow).)

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RNF19A, Polyclonal Antibody (Cat# AAA288911)

Full Name

RNF19A Antibody (Center)

Gene Names

RNF19A; RNF19

Reactivity

Human (Predicted Reactivity: Mouse, Pig)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using ZNF265 antibody.)

WB (Western Blot) (Western blot analysis of lysates from HT-1080,HL-60 cell line (from left to right),using ZNF265 Antibody(AAA288169). AAA288169 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

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ZNF265, Polyclonal Antibody (Cat# AAA288169)

Full Name

ZNF265 Antibody

Gene Names

ZRANB2; ZIS; ZIS1; ZIS2; ZNF265

Reactivity

Human, mouse, rat

Applications

Immunofluorescence, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (RGS5 Antibody (N-term) (AAA288173)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RGS5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (RGS5 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RGS5 antibody detected the RGS5 protein (arrow).)

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RGS5, Polyclonal Antibody (Cat# AAA288173)

Full Name

RGS5 Antibody (N-term)

Gene Names

RGS5; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (IMPDH2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (IMPDH2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMPDH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of IMPDH2 Antibody (Center) in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane). IMPDH2 (arrow) was detected using the purified Pab.)

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IMPDH2, Polyclonal Antibody (Cat# AAA289491)

Full Name

IMPDH2 Antibody (Center)

Gene Names

IMPDH2; IMPD2; IMPDH-II

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat, Hamster, Zebrafish)

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 Antibody. The picture on the right is blocked with the synthesized peptide.)

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Rac1/CDC42, Polyclonal Antibody (Cat# AAA317103)

Full Name

Rac1/CDC42 Antibody

Gene Names

RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO and Jurkat cells, using GluR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GluR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using GluR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GluR1, Polyclonal Antibody (Cat# AAA317059)

Full Name

GluR1 Antibody

Gene Names

GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (AAA320752 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on HeLa cell lysate using Cyclin H Antibody.The lane on the left is treated with the antigen-specific peptide.)

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Cyclin H, Polyclonal Antibody (Cat# AAA320752)

Full Name

Cyclin H Antibody

Gene Names

CCNH; CAK; p34; p37; CycH

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis on A549 cell lysate using Claudin 5 Antibody, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA320778 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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Claudin 5, Polyclonal Antibody (Cat# AAA320778)

Full Name

Claudin 5 Antibody

Gene Names

CLDN5; AWAL; BEC1; TMVCF; TMDVCF; CPETRL1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA320896 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA320896 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA320896) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)

WB (Western Blot) (Western blot analysis on HuvEc cell lysate using Sodium Channel-pan Antibody, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis on rat muscle tissue lysate using Sodium Channel-pan Antibody)

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Sodium Channel-pan, Polyclonal Antibody (Cat# AAA320896)

Full Name

Sodium Channel-pan Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa and HUVEC cells, using Parkin Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Parkin Antibody. The picture on the right is blocked with the synthesized peptide.)

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Parkin, Polyclonal Antibody (Cat# AAA316787)

Full Name

Parkin Antibody

Gene Names

PRKN; PDJ; AR-JP; LPRS2; PARK2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of STEAP1 antibody in paraffin-embedded human prostate carcinoma tissue.)

WB (Western Blot) (Western blot analysis of lysate from A549, using STEAP1 antibody.)

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STEAP1, Polyclonal Antibody (Cat# AAA318494)

Full Name

STEAP1 Antibody

Gene Names

STEAP1; STEAP; PRSS24

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (AAA321586 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA321586 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of CDC25B phosphorylation expression in etoposide treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-CDC25B (Ser353) Antibody expression in etoposide treated COS7 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321586 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 33 degree C)

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CDC25B, Polyclonal Antibody (Cat# AAA321586)

Full Name

Phospho-CDC25B (Ser353) Antibody

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemistry) (AAA322418 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of IRF3 expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322418 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA322418 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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IRF3, Polyclonal Antibody (Cat# AAA322418)

Full Name

IRF3 Antibody

Gene Names

IRF3; IIAE7

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322456 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of WNK1 expression in EGF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322456 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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WNK1, Polyclonal Antibody (Cat# AAA322456)

Full Name

WNK1 Antibody

Gene Names

WNK1; KDP; PSK; p65; HSN2; HSAN2; PRKWNK1; PPP1R167

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA322769 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of LTBP1 using HeLa whole cell lysates)

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LTBP1, Polyclonal Antibody (Cat# AAA322769)

Full Name

LTBP1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (IHC image of diluted at 1:10 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of diluted at 1:10 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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HIST1H2AG, Polyclonal Antibody (Cat# AAA244074)

Full Name

Rabbit anti-Human HIST1H2AG Polyclonal Antibody

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from various samples, using CHMP4B antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: Rat brain; Lane 3: Mouse brain;)

IHC (Immunohiostchemistry) (AAA323080 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of mouse heart tissue, using CHMP4B antibody.)

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CHMP4B, Polyclonal Antibody (Cat# AAA323080)

Full Name

CHMP4B Antibody

Gene Names

CHMP4B; SNF7; CTPP3; Shax1; CHMP4A; SNF7-2; VPS32B; CTRCT31; Vps32-2; C20orf178; dJ553F4.4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA323973 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells using CBF beta antibody.)

IHC (Immunohiostchemistry) (AAA323973 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from 293, using CBF beta Antibody. Lane 1 was treated with the blocking peptide.)

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CBF beta, Polyclonal Antibody (Cat# AAA323973)

Full Name

CBF beta Antibody

Gene Names

CBFB; PEBP2B

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from rat spleen, using MLH3 antibody.)

WB (Western Blot) (Western blot analysis of extracts from K562, using MLH3 antibody.)

IHC (Immunohistochemisry) (AAA324307 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohiostchemistry) (AAA324307 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

IF (Immunofluorescence) (AAA324307 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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MLH3, Polyclonal Antibody (Cat# AAA324307)

Full Name

MLH3 Antibody

Gene Names

MLH3; HNPCC7

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Detection of human SAMHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody AAA212262 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by rabbit anti-SAMHD1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)

IHC (Immunohistochemistry) (Detection of human SAMHD1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SAMHD1 (Cat. No. AAA212262 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)

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SAMHD1, Polyclonal Antibody (Cat# AAA212262)

Full Name

Rabbit anti-SAMHD1 Antibody, Affinity Purified

Gene Names

SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $605/0.1 mg $940/5x0.01 mg $1,170/2x0.1 mg $1,710/3x0.1 mg $2,220/4x0.1 mg $2,475/5x0.1 mg

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WB (Western Blot) (Host: RabbitTarget Name: VEGFASample Type: Jurkat Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-VEGFA AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

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VEGFA, Polyclonal Antibody (Cat# AAA201045)

Full Name

VEGFA Antibody - C-terminal region

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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IHC (Immunohiostchemistry) (Anti- Bag3 Picoband antibody, AAA46393, IHC(P)IHC(P): Human Lung Cancer Tissue)

WB (Western Blot) (Anti- Bag3 Picoband antibody, AAA46393, Western blottingAll lanes: Anti Bag3 (AAA46393) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 62KDObserved bind size: 62KD)

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Bag3, Polyclonal Antibody (Cat# AAA46393)

Full Name

Anti-Bag3 Antibody

Gene Names

BAG3; BIS; MFM6; BAG-3; CAIR-1

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of VISA in Human Brain cells with VISA antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of VISA in human brain tissue with VISA antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

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VISA, Polyclonal Antibody (Cat# AAA39613)

Full Name

VISA Antibody

Gene Names

MAVS; IPS1; VISA; IPS-1; CARDIF

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

VISA Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL.)

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PDCD4, Polyclonal Antibody (Cat# AAA39991)

Full Name

PDCD4 Antibody

Gene Names

PDCD4; H731

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

PDCD4 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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WB (Western Blot) (Western blot of extracts from HUVEC cells, treated with PMA 125 ng/ml 30', using PKD1/PKC mu antibody. The lane on the right is treated with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence of A549 cells, using PKD1/PKC mu antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Anti-PKC Mu antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)

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PRKD1 / PKD Mu, Polyclonal Antibody (Cat# AAA51419)

Full Name

Rabbit Polyclonal (IgG) to Human PRKD1 / PKD Mu

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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IHC (Immunohiostchemistry) (AAA326712 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of Mouse spleen lysate, using FOXM1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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FOXM1, Polyclonal Antibody (Cat# AAA326712)

Full Name

FOXM1 Antibody

Gene Names

FOXM1; MPP2; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1A; FOXM1B; FOXM1C; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from HT-29 cells using HOXA6 antibody.)

IF (Immunofluorescence) (AAA324790 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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HOXA6, Polyclonal Antibody (Cat# AAA324790)

Full Name

HOXA6 Antibody

Gene Names

HOXA6; HOX1; HOX1B; HOX1.2

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA324709 staining MCF7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohiostchemistry) (AAA324709 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from MCF-7 cells, using ELOVL5 antibody.)

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ELOVL5, Polyclonal Antibody (Cat# AAA324709)

Full Name

ELOVL5 Antibody

Gene Names

ELOVL5; HELO1; SCA38; dJ483K16.1

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA327360 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of AGTR2 using LOVO whole lysates.)

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AGTR2, Polyclonal Antibody (Cat# AAA327360)

Full Name

AGTR2 Antibody

Gene Names

AGTR2; AT2; ATGR2; MRX88

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Peptide affinity purification

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of lysates from MCF7 cells, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The lane on the left is blocked with the phospho peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The picture on the right is blocked with the phospho peptide.)

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Estrogen Receptor-alpha, Polyclonal Antibody (Cat# AAA316490)

Full Name

Estrogen Receptor-alpha (Phospho-Ser106) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using GPR137C Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GPR137C Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using GPR137C Antibody. The picture on the right is blocked with the synthesized peptide.)

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GPR137C, Polyclonal Antibody (Cat# AAA318090)

Full Name

GPR137C Antibody

Gene Names

GPR137C; TM7SF1L2

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29 and K562 cells, using VIPR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using VIPR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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VIPR1, Polyclonal Antibody (Cat# AAA318234)

Full Name

VIPR1 Antibody

Gene Names

VIPR1; II; HVR1; RDC1; V1RG; VIPR; VIRG; VAPC1; VPAC1; VPAC1R; VIP-R-1; VPCAP1R; PACAP-R2; PACAP-R-2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohistochemisry) (AAA323526 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of DYRK4 expression in NCI-H292 cell line lysates ; developed using the ECL technique, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA323526 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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DYRK4, Polyclonal Antibody (Cat# AAA323526)

Full Name

DYRK4 Antibody

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of mouse testis tissue, using DIS3 antibody.)

IHC (Immunohistochemistry) (AAA323207 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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DIS3, Polyclonal Antibody (Cat# AAA323207)

Full Name

DIS3 Antibody

Gene Names

DIS3; RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of OR7E24 expression in 721_B whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA323624 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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OR7E24, Polyclonal Antibody (Cat# AAA323624)

Full Name

OR7E24 Antibody

Gene Names

OR7E24; HSHT2; OR19-8; OR7E24P; OR7E24Q

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA326086 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA326086 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of MCF7 cell lysate, using CDC25A Antibody. The lane on the left is treated with the antigen-specific peptide.)

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CDC25A, Polyclonal Antibody (Cat# AAA326086)

Full Name

CDC25A Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (Detection of mouse DHX9 by immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. AAA213837) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human DHX9 by immunohistochemistry. Sample: FFPE section of human stomach adenocarcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. AAA213837) used at a dilution of 1:250. Detection: DAB)

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DHX9, Polyclonal Antibody (Cat# AAA213837)

Full Name

Rabbit anti-DHX9 IHC Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.0025 mg $630/8 mL $940/5x0.0025 mg $1,220/2x8 mL $1,780/3x8 mL $2,315/4x8 mL $2,620/5x0.025 mg

WB (Western Blot)

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Lysozyme, Polyclonal Antibody (Cat# AAA71338)

Full Name

Rabbit anti Lysozyme Polyclonal Antibody

Gene Names

LYZ; LZM; LYZF1

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Western Blot

Purity

The Rabbit IgG is purified by Epitope Affinity Purification.

Pricing

$365/0.1 mg $705/2x0.1 mg $1,030/3x0.1 mg $1,335/4x0.1 mg $1,420/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of CAPN6 in Human Lung cells with CAPN6 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of CAPN6 in human lung tissue with CAPN6 antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/mL.)

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CAPN6, Polyclonal Antibody (Cat# AAA40123)

Full Name

CAPN6 Antibody

Gene Names

CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

CAPN6 Antibody is Protein A purified.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of RTP801 in mouse kidney tissue with RTP801 antibody at 20 μg/mL.Red: RTP801 Antibody (4495)Blue: DAPI staining)

IHC (Immunohiostchemistry) (Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 μg/mL.)

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RTP801, Polyclonal Antibody (Cat# AAA40135)

Full Name

RTP801 Antibody

Gene Names

DDIT4; Dig2; REDD1; REDD-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

RTP801 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

WB (Western Blot) (Western blot analysis of extracts of A-20 cell lines, using SLPI antibody.)

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SLPI, Polyclonal Antibody (Cat# AAA37068)

Full Name

SLPI Polyclonal Antibody

Gene Names

SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.)

WB (Western Blot) (Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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SIK3, Polyclonal Antibody (Cat# AAA39219)

Full Name

SIK3 Antibody

Gene Names

SIK3; L19; QSK; SIK-3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

SIK3 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of 293T cells using anti-PDIP/PDIA2 antibody (AAA126295).Overlay histogram showing 293T cells stained with AAA126295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIP/PDIA2 Antibody (AAA126295, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PDIP/PDIA2 using anti-PDIP/PDIA2 antibody (AAA126295).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human PCP tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIP/PDIA2 antigen affinity purified polyclonal antibody (#AAA126295) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDIP/PDIA2 at approximately 65 kDa. The expected band size for PDIP/PDIA2 is at 68 kDa.)

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PDIP/PDIA2, Polyclonal Antibody (Cat# AAA126295)

Full Name

Anti-PDIP/PDIA2 Antibody Picoband

Gene Names

PDIA2; PDI; PDA2; PDIP; PDIR

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Triadin/TRDN antibody (AAA126474).Overlay histogram showing MCF-7 cells stained with AAA126474 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Triadin/TRDN Antibody (AAA126474, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of Triadin/TRDN using anti-Triadin/TRDN antibody (AAA126474).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Triadin/TRDN antigen affinity purified polyclonal antibody (#AAA126474) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Triadin/TRDN at approximately 25 kDa. The expected band size for Triadin/TRDN is at 82 kDa.)

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Triadin/TRDN, Polyclonal Antibody (Cat# AAA126474)

Full Name

Anti-Triadin/TRDN Antibody Picoband

Gene Names

TRDN; TDN; TRISK; TRISK51; MGC88285; DKFZp779I2253

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CBS antibody (AAA125963).Overlay histogram showing CACO-2 cells stained with AAA125963 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBS Antibody (AAA125963, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of CBS using anti-CBS antibody (AAA125963).CBS was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CBS Antibody (AAA125963) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of CBS using anti-CBS antibody (AAA125963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: huamn HEK293 whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human K562 whole cell lysates,Lane 6: rat liver tissue lysates,Lane 7: rat pancreas tissue lysates,Lane 8: mouse liver tissue lysates,Lane 9: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (#AAA125963) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CBS at approximately 63 kDa. The expected band size for CBS is at 63 kDa.)

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CBS, Polyclonal Antibody (Cat# AAA125963)

Full Name

Anti-CBS Antibody Picoband

Gene Names

CBS; HIP4

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IP (Immunoprecipitation) (SAMM50 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-SAMM50 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SAMM50 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size :55 kDa)

IHC (Immunohiostchemistry) (Immunochemical staining of human SAMM50 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-SAMM50 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))

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SAMM50, Polyclonal Antibody (Cat# AAA256635)

Full Name

Anti-SAMM50 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A & Antigen Affinity

Pricing

$380/0.1 mL $735/2x0.1 mL $1,075/3x0.1 mL $1,390/4x0.1 mL $1,495/5x0.1 mL

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IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using AAA241298(JAG1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using AAA241298(JAG1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

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JAG1, Polyclonal Antibody (Cat# AAA241298)

Full Name

JAG1 Antibody

Gene Names

JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)

WB (Western Blot) (Gel: 10%SDS-PAGE Lysates (from left to right): Mouse testis tissue Amount of lysate: 20ug per lane Primary antibody: 1/100 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

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ADORA3, Polyclonal Antibody (Cat# AAA242707)

Full Name

ADORA3 Antibody

Gene Names

ADORA3; A3AR; AD026; bA552M11.5; RP11-552M11.7

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,485/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.