WB (Western Blot)
(Detection of mouse UBE2O by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CT26, 3T3-L1, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse UBE2O Polyclonal Antibody | anti-UBE2O antibody
Rabbit anti-UBE2O Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
UBE2O, Antibody; Rabbit anti-UBE2O Antibody, Affinity Purified; ubiquitin conjugating enzyme E2O; ubiquitin-protein ligase O; ubiquitin-conjugating enzyme E2-230K; Ubiquitin-conjugating enzyme E2 O; ubiquitin carrier protein O; E2-230K; E2/E3 hybrid ubiquitin-protein ligase UBE2O; (E3-independent) E2 ubiquitin-conjugating enzyme; ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin conjugating enzyme E2 O; anti-UBE2O antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-UBE2O antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 725 and 775
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse UBE2O by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CT26, 3T3-L1, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse UBE2O by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CT26, 3T3-L1, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human UBE2O by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, GaMG, Jurkat, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human UBE2O by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, GaMG, Jurkat, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human UBE2O by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for IP at 6 ug per reaction. UBE2O was also immunoprecipitated by a previous lot of this antibody (AAA211659 lot 3) and a second antibody against a different epitope of UBE2O (Antibody 2). For blotting immunoprecipitated UBE2O, AAA211659 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human UBE2O by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE2O antibody (AAA211659 lot 4) used for IP at 6 ug per reaction. UBE2O was also immunoprecipitated by a previous lot of this antibody (AAA211659 lot 3) and a second antibody against a different epitope of UBE2O (Antibody 2). For blotting immunoprecipitated UBE2O, AAA211659 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-UBE2O antibody
UBE2O (ubiquitin-conjugating enzyme E2O) is an E2 ubiquitin conjugating enzyme that is part of the three-step pathway responsible for the covalent attachment of ubiquitin to proteins for their targeted degradation.
Product Categories/Family for anti-UBE2O antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The UBE2O ube2o (Catalog #AAA211659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UBE2O Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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