WB (Western Blot)
(Detection of human DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody AAA212183 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
Rabbit anti-Human DCTN2 Polyclonal Antibody | anti-DCTN2 antibody
Rabbit anti-DCTN2 Antibody, Affinity Purified
Gene Names
DCTN2; RBP50; DCTN50; HEL-S-77; DYNAMITIN
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DCTN2, Antibody; Rabbit anti-DCTN2 Antibody, Affinity Purified; dynactin 2 (p50); RBP50; p50 dynamitin; HEL-S-77; epididymis secretory sperm binding protein; epididymis secretory protein Li 77; DYNAMITIN; dynactin complex 50 kDa subunit; DCTN-50; DCTN50; 50 kDa dynein-associated polypeptide; dynactin subunit 2; Dynactin subunit 2; anti-DCTN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DCTN2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 351 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody AAA212183 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody AAA212183 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human DCTN2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DCTN2 (Cat. No. AAA212183 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human DCTN2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DCTN2 (Cat. No. AAA212183 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-DCTN2 antibody
Dynactin subunit 2 (DCTN2) modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. DCTN2 is involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13561].
Product Categories/Family for anti-DCTN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dynactin subunit 2 isoform 1
NCBI Official Synonym Full Names
dynactin subunit 2
NCBI Official Symbol
DCTN2
NCBI Official Synonym Symbols
RBP50; DCTN50; HEL-S-77; DYNAMITIN
NCBI Protein Information
dynactin subunit 2
UniProt Protein Name
Dynactin subunit 2
UniProt Gene Name
DCTN2
UniProt Synonym Gene Names
DCTN50; DCTN-50
UniProt Entry Name
DCTN2_HUMAN
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Product Notes
The DCTN2 dctn2 (Catalog #AAA212183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DCTN2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCTN2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DCTN2 dctn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCTN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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