Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us

Filters


Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.


Viewing 4850-4900 of 5299 product results


product-image-AAA111824_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr301/302) antibody.)

A-RAF, Antibody (Cat# AAA111824)

Full Name
A-RAF (Phospho-Tyr302) Antibody
Gene Names
ARAF; PKS2; A-RAF; ARAF1; RAFA1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111841_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) antibody. Western blot analysis of extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins), using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) antibody.)

PKD1/2/3/PKC, Antibody (Cat# AAA111841)

Full Name
PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody
Gene Names
PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111862_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PFKFB2 (Phospho-Ser483) antibody. Western blot analysis of extracts from 293 cells, treated with Heat shock, using PFKFB2 (Phospho-Ser483) antibody.)

PFKFB2, Antibody (Cat# AAA111862)

Full Name
PFKFB2 (Phospho-Ser483) Antibody
Gene Names
PFKFB2; PFK-2/FBPase-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111863_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using PPAR-beta (Phospho-Thr1457) antibody.)

PPAR-BP, Antibody (Cat# AAA111863)

Full Name
PPAR-BP (Phospho-Thr1457) Antibody
Gene Names
MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111874_IHC13.jpg IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) antibody. Immunofluorescence analysis of HeLa cells, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) antibody.)

DDX5/DEAD-box, Antibody (Cat# AAA111874)

Full Name
DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody
Gene Names
DDX5; p68; HLR1; G17P1; HUMP68
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111956_WB13.jpg WB (Western Blot) (Western blot analysis of the extracts from NIH/3T3 cells using HDAC5 (Ab-498) Antibody and HDAC5 (Phospho-Ser498) Antibody.)

HDAC5, Antibody (Cat# AAA111956)

Full Name
HDAC5 (Phospho-Ser498) Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111973_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using Bcr (Phospho-Tyr360) antibody.)

Bcr, Antibody (Cat# AAA111973)

Full Name
Bcr (Phospho-Tyr360) Antibody
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112019_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using Ku80 (Phospho-Thr714) Antibody (#A0450).)

Ku80, Antibody (Cat# AAA112019)

Full Name
Ku80 (Phospho-Thr714) Antibody
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112027_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using Pin1 (Phospho-Ser16) antibody. Western blot analysis of extracts from COS-7 cells, treated with insulin (0.01U/ml, 15mins), using Pin1 (Phospho-Ser16) antibody.)

Pin1, Antibody (Cat# AAA112027)

Full Name
Pin1 (Phospho-Ser16) Antibody
Gene Names
PIN1; DOD; UBL5
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111812_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.)

SAPK/JNK, Antibody (Cat# AAA111812)

Full Name
SAPK/JNK (Phospho-Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110958_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with PMA. Left: Using ERK1/2 (phospho-Thr202/Tyr204) antibody. Right: Using the same antibody preincubated with synthesized peptide.)

ERK1/2, Antibody (Cat# AAA110958)

Full Name
ERK1/2 (Phospho-Thr202/Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110960_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from A2780 cells (Lane 1 and 2) and MDA-MB-435 cells (Lane 3), using CDK2 (phospho-Thr160) antibody.)

CDK2, Antibody (Cat# AAA110960)

Full Name
CDK2 (Phospho-Thr160) Antibody
Gene Names
CDK2; CDKN2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110961_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from HUVEC cells, using ICAM-1 (Ab-512) Antibody and ICAM-1 (Phospho-Tyr512) Antibody.)

ICAM-1, Antibody (Cat# AAA110961)

Full Name
ICAM-1(Phospho-Tyr512) Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110968_WB13.jpg WB (Western Blot) (UV-+ Western blot analysis of extracts from NIH/3T3 cells using c-Jun (phospho-Ser63) antibody)

c-Jun, Antibody (Cat# AAA110968)

Full Name
c-Jun (Phospho-Ser63) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110975_WB13.jpg WB (Western Blot) (Western blot analysis of extract from Jurkat cells, untreated or treated with PMA (1ng/ml, 5min), using Pyk2 (Ab-402) antibody and Pyk2 (phospho-Tyr402) antibody.)

Pyk2, Antibody (Cat# AAA110975)

Full Name
Pyk2 (Phospho-Tyr402) Antibody
Gene Names
PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110981_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells treated with UV (20min), using BRCA1 (phospho-Ser1423) antibody.)

BRCA1, Antibody (Cat# AAA110981)

Full Name
BRCA1 (Phospho-Ser1423) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110984_WB13.jpg WB (Western Blot) (Western blot analysis of extract from SW 626 cells, using beta-Catenin (Ab-37) antibody and beta-Catenin (phospho-Ser37) antibody.)

beta-Catenin, Antibody (Cat# AAA110984)

Full Name
beta-Catenin (Phospho-Ser37) Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110998_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kappaB p65 (Ab-529) antibody and NF-kappaB p65 (phospho-Ser529) antibody.)

NFkappaB-p65, Antibody (Cat# AAA110998)

Full Name
NFkappaB-p65 (Phospho-Ser529) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111000_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with Heat Shock or UV, using HSP27 (Ab-82) antibody and HSP27 (Phospho-Ser82) antibody.)

HSP27, Antibody (Cat# AAA111000)

Full Name
HSP27 (Phospho-Ser82) Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111013_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from ovary cancer cells using Myc (phospho-Thr58) antibody.)

Myc, Antibody (Cat# AAA111013)

Full Name
Myc (Phospho-Thr58) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111019_IHC15.gif IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 (phospho-Tyr221) antibody.)

JAK2, Antibody (Cat# AAA111019)

Full Name
JAK2 (Phospho-Tyr221) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111021_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using JunB (phospho-Ser259) antibody.)

JunB, Antibody (Cat# AAA111021)

Full Name
JunB (Phospho-Ser259) Antibody
Gene Names
JUNB; AP-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111022_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from MCF7 cells, using HSF1 (phospho-Ser303) antibody.)

HSF1, Antibody (Cat# AAA111022)

Full Name
HSF1 (phospho-Ser303) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111032_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with TNF-alpha using NF-kappaB p105/p50 (phospho-Ser907) antibody.)

NFkappaB-p105/p50, Antibody (Cat# AAA111032)

Full Name
NFkappaB-p105/p50 (Phospho-Ser907) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110685_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with Forskolin (40nM, 30mins), using DARPP-32 (phospho-Thr75) antibody.)

DARPP-32, Antibody (Cat# AAA110685)

Full Name
DARPP-32 (Phospho-Thr75) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110702_WB13.jpg WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody. Immunofluorescence analysis of HuvEc cells, using Kir6.2 (Phospho-Thr224) antibody.)

Kir6.2, Antibody (Cat# AAA110702)

Full Name
Kir6.2 (Phospho-Thr224) Antibody
Gene Names
KCNJ11; BIR; HHF2; PHHI; IKATP; TNDM3; KIR6.2; MODY13
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110703_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with serum (20%, 15mins), using OSR1 (Phospho-Thr185) antibody.)

OSR1, Antibody (Cat# AAA110703)

Full Name
OSR1 (Phospho-Thr185) Antibody
Gene Names
OXSR1; OSR1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110478_AD13.png Application Data (P-peptide-+Immunohistochemistry analysis of paraffin-embedded human lung carcinoma using CDC25A (Phospho-Ser75) Antibody.Western blot analysis of extracts from HuvEc, using CDC25A (Phospho-Ser75) Antibody.)

CDC25A, Antibody (Cat# AAA110478)

Full Name
CDC25A (Phospho-Ser75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
Pricing
product-image-AAA110515_AD11.jpg Application Data (p16-INK4a (Phospho-Ser152) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

p16-INK4a, Antibody (Cat# AAA110515)

Full Name
p16-INK4a (Phospho-Ser152) Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human (Identities = 100%, Positives = 100%)
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110522_WB13.jpg WB (Western Blot) (Peptide-+ Western blot analysis of extracts from HT-29 cells, using IRS-1 (Phospho-Ser312) antibody. Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) antibody.)

IRS-1, Antibody (Cat# AAA110522)

Full Name
IRS-1 (Phospho-Ser312) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110529_AD11.png Application Data (P-peptide - +Immunofluorescence analysis of A549 cells, using Akt (Phospho-Ser473) Antibody)

Akt, Antibody (Cat# AAA110529)

Full Name
Akt (Phospho-Ser473) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA109174_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mnk1 (Phospho-Thr385) antibody. Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Phospho-Thr385) antibody.)

Mnk1, Antibody (Cat# AAA109174)

Full Name
Mnk1 (Phospho-Thr385) Antibody
Gene Names
MKNK1; MNK1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109201_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using Mst1/2 (Phospho-Thr183) antibody. Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1/2 (Phospho-Thr183) antibody.)

Mst1/2, Antibody (Cat# AAA109201)

Full Name
Mst1/2 (Phospho-Thr183) Antibody
Gene Names
STK4; KRS2; MST1; YSK3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109214_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), using HSL (Phospho-Ser855/554) antibody (#A0074).)

HSL, Antibody (Cat# AAA109214)

Full Name
HSL (Phospho-Ser855/554) Antibody
Gene Names
LIPE; HSL; LHS; AOMS4; FPLD6
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110564_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody. Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody.)

BLNK, Antibody (Cat# AAA110564)

Full Name
BLNK (Phospho-Tyr84) Antibody
Gene Names
BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110579_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody. Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using TOB1 (Phospho-Ser164) antibody.)

TOB1, Antibody (Cat# AAA110579)

Full Name
TOB1 (Phospho-Ser164) Antibody
Gene Names
TOB1; TOB; TROB; APRO5; APRO6; PIG49; TROB1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatographyusing non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110623_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody. Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody.)

UBF, Antibody (Cat# AAA110623)

Full Name
UBF (Phospho-Ser484) Antibody
Gene Names
UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111066_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells using GATA-1 (phospho-Ser142) antibody.)

GATA1, Antibody (Cat# AAA111066)

Full Name
GATA1 (Phospho-Ser142) Antibody
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111072_IHC15.jpg IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 (phospho-Tyr1007) antibody.)

JAK2, Antibody (Cat# AAA111072)

Full Name
JAK2 (Phospho-Tyr1007) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111075_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser795) antibody.)

Rb, Antibody (Cat# AAA111075)

Full Name
Rb (Phospho-Ser795) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111089_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells treated with TNF-alpha and using NF-kappaB p105/p50 (phospho-Ser337) antibody.)

NF-kappaB p105/p50, Antibody (Cat# AAA111089)

Full Name
NF-kappaB p105/p50 (Phospho-Ser337) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111090_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody and ADD1 (Phospho-Ser726) antibody.)

ADD1, Antibody (Cat# AAA111090)

Full Name
ADD1 (Phospho-Ser726) Antibody
Gene Names
ADD1; ADDA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111094_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells using ASK1 (phospho-Ser83) antibody.)

ASK1, Antibody (Cat# AAA111094)

Full Name
ASK1 (Phospho-Ser83) Antibody
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111102_IHC15.jpg IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (phospho-Tyr1248) antibody.)

HER2, Antibody (Cat# AAA111102)

Full Name
HER2 (Phospho-Tyr1248) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111103_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with UV using c-Jun (phospho-Thr91) antibody.)

c-Jun, Antibody (Cat# AAA111103)

Full Name
c-Jun (Phospho-Thr91) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111108_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from A2780 cells using cdc25A (Ab-75) antibody and cdc25A (phospho-Ser75) antibody)

cdc25A, Antibody (Cat# AAA111108)

Full Name
cdc25A (Phospho-Ser75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111110_WB13.jpg WB (Western Blot) (Western blot analysis of extract from 293 cells, untreated or treated with insulin (40uM, 30min), using GSK3beta (Ab-216) Antibody and GSK3beta (Phospho-Tyr216) Antibody.)

GSK3beta, Antibody (Cat# AAA111110)

Full Name
GSK3beta (Phospho-Tyr216) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

IGF-1R, Antibody (Cat# AAA111114)

Full Name
IGF-1R (Phospho-Tyr1280) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110885_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells using BAD (phospho-Ser155) antibody.)

BAD, Antibody (Cat# AAA110885)

Full Name
BAD (Phospho-Ser155) Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110891_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells using NF-kappaB p65 (phospho-Ser276) antibody.)

NFkappaB-p65, Antibody (Cat# AAA110891)

Full Name
NFkappaB-p65 (Phospho-Ser276) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

What Are Phospho Antibodies?


Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.


Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.


What Are The Key Applications of Phospho Antibodies?


1. Western Blotting


One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.


2. Staining of Fixed Cells (Immunocytochemistry)


Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.


3. Specificity Tests Using Peptides


In order to make sure that the antibody is only binding to the right target:


  • Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).

  • If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.

  • A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.

  • Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.


4. Genetic Confirmation


As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.


Why Buy Phospho Antibodies Through Us?


  • The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:

  • Standard methods and proper controls in all tests to ensure high quality.

  • These antibodies are tested and validated in different cell types and species.

  • High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.


FAQ


1. What Are Phospho-Specific Antibodies?


Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.


2. How to Detect Phosphorylated Proteins in a Western Blot?


To find out if a protein is phosphorylated using Western blot:


  • Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.

  • You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).


3. How to Choose the Best Antibody?


Here are some simple tips to help you pick the right antibody:


  • Know your target

  • Match your sample characteristics

  • Confirm the intended use is appropriate

  • Check “host” and “type”

  • Check the “quality” of the presented data/images

  • Appraise whether the available validation meets your needs


Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.