Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG (1ug) instead of AAA235567 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA235567 (3ug)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20ug))

IHC (Immunohistochemisry) (IHC image of AAA235567 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry) (IHC image of AAA235567 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in Hela whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not)All lanes Phospho-JAK2 antibody at 0.75ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120 KDaObserved band size: 120 KDa)

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JAK2, Monoclonal Recombinant Antibody (Cat# AAA235567)

Full Name

Phospho-JAK2 (Y1007 + Y1008) Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-LAT in A549 whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA012767A191phHU in A549 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA012767A191phHU(3ug)+ A549 whole cell lysate(1mg)Lane 3: A549 whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemisry) (IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry) (IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Jurkat whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)All lanes?Phospho-LAT antibody at 2.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

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LAT, Monoclonal Recombinant Antibody (Cat# AAA235569)

Full Name

Phospho-LAT (Y191) Antibody

Gene Names

LAT; LAT1; pp36; IMD52

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA013456A185phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA013456A185phHU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate,HepG2 whole cell lysate(treated with EGF or not)All lanes?Phospho-MAPK3 antibody at 2.35ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDa)

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MAPK3, Monoclonal Recombinant Antibody (Cat# AAA235571)

Full Name

Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with EGF or not)All lanes?Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46,54 KDaObserved band size: 46,54 KDa)

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MAPK8/MAPK9/MAPK10, Monoclonal Recombinant Antibody (Cat# AAA235572)

Full Name

Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with CSB-RA015761A32phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-NFKBIA antibody at 1.07ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39 KDaObserved band size: 39 KDa)

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NFKBIA, Monoclonal Recombinant Antibody (Cat# AAA235576)

Full Name

Phospho-NFKBIA (S32) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI; EDAID2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-AP-1 (T239) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-AP-1 (T239) Polyclonal Antibody)

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JUN, Polyclonal Antibody (Cat# AAA235766)

Full Name

Phospho-JUN (T239) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of VEC A549 cells using Phospho-ATF-2 (S62) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-ATF-2 (S62) Polyclonal Antibody)

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ATF2, Polyclonal Antibody (Cat# AAA235769)

Full Name

Phospho-ATF2 (S62) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-Catenin-beta (S37) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Catenin-beta (S37) Polyclonal Antibody)

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CTNNB1, Polyclonal Antibody (Cat# AAA235772)

Full Name

Phospho-CTNNB1 (S37) Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-Cdc2 (T161) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-Cdc2 (T161) Polyclonal Antibody)

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CDK1, Polyclonal Antibody (Cat# AAA235775)

Full Name

Phospho-CDK1 (T161) Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of VEC 293 A549 cells using Phospho-Cdc25B (S323) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Cdc25B (S323) Polyclonal Antibody)

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CDC25B, Polyclonal Antibody (Cat# AAA235776)

Full Name

Phospho-CDC25B (S323) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of VEC 293 A549 cells using Phospho-Cdc25C (S216) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HuvEc cells using Phospho-Cdc25C (S216) Polyclonal Antibody)

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CDC25C, Polyclonal Antibody (Cat# AAA235777)

Full Name

Phospho-CDC25C (S216) Antibody

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of Jurkat-UV cells using Phospho-Chk2 (T68) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-Chk2 (T68) Polyclonal Antibody)

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CHEK2, Polyclonal Antibody (Cat# AAA235779)

Full Name

Phospho-CHEK2 (T68) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-Connexin 43 (S368) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HuvEc cells using Phospho-Connexin 43 (S368) Polyclonal Antibody)

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GJA1, Polyclonal Antibody (Cat# AAA235781)

Full Name

Phospho-GJA1 (S368) Antibody

Gene Names

GJA1; HSS; CX43; GJAL; ODDD; AVSD3; HLHS1; DFNB38

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-c-Src (Y529) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-c-Src (Y529) Polyclonal Antibody)

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SRC, Polyclonal Antibody (Cat# AAA235783)

Full Name

Phospho-SRC (Y529) Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of KB MCF7 cells using Phospho-ERK 1/2 (T202) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-ERK 1/2 (T202) Polyclonal Antibody)

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MAPK3/MAPK1, Polyclonal Antibody (Cat# AAA235788)

Full Name

Phospho-MAPK3/MAPK1 (T202) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-ERK 1/2 (Y204) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-ERK 1/2 (Y204) Polyclonal Antibody)

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MAPK3/MAPK1, Polyclonal Antibody (Cat# AAA235789)

Full Name

Phospho-MAPK3/MAPK1 (Y204) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of VEC 293 cells using Phospho-HDAC8 (S39) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-HDAC8 (S39) Polyclonal Antibody)

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HDAC8, Polyclonal Antibody (Cat# AAA235797)

Full Name

Phospho-HDAC8 (S39) Antibody

Gene Names

HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-Histone H3 (S10) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-Histone H3 (S10) Polyclonal Antibody)

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Histone H3, Polyclonal Antibody (Cat# AAA235798)

Full Name

Phospho-Histone H3 (S10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody)

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IGF1R, Polyclonal Antibody (Cat# AAA235801)

Full Name

Phospho-IGF1R (Y1165/Y1166) Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-IKKalpha (T23) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-IKKalpha (T23) Polyclonal Antibody)

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CHUK, Polyclonal Antibody (Cat# AAA235802)

Full Name

Phospho-CHUK (T23) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of various cells using Phospho-IRS-1 (S794) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Rat cells using Phospho-IRS-1 (S794) Polyclonal Antibody)

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IRS1, Polyclonal Antibody (Cat# AAA235804)

Full Name

Phospho-IRS1 (S794) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of various cells using Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody)

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NFKBIA, Polyclonal Antibody (Cat# AAA235805)

Full Name

Phospho-NFKBIA (S32/S36) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of customer's lysis using Phospho-mTOR (S2448) antibody.)

WB (Western Blot) (Western Blot analysis of HY926 cells using Phospho-mTOR (S2448) Polyclonal Antibody)

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MTOR, Polyclonal Antibody (Cat# AAA235812)

Full Name

Phospho-MTOR (S2448) Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-NFkappaB-p65 (S536) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-NFkappaB-p65 (S536) Polyclonal Antibody)

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RELA, Polyclonal Antibody (Cat# AAA235814)

Full Name

Phospho-RELA (S536) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of customer's using Phospho-p53 (T18) Polyclonal Antibody.)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-p53 (T18) Polyclonal Antibody)

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TP53, Polyclonal Antibody (Cat# AAA235816)

Full Name

Phospho-TP53 (T18) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HELA PC3 MCF7 22RV1 cells using Phospho-PKC zeta (T410) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 823 cells using Phospho-PKC zeta (T410) Polyclonal Antibody)

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PRKCZ, Polyclonal Antibody (Cat# AAA235819)

Full Name

Phospho-PRKCZ (T410) Antibody

Gene Names

PRKCZ; PKC2; PKC-ZETA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of SH-SY5Y MOUSE-BRAIN cells using Phospho-Stat1 (Y701) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-Stat1 (Y701) Polyclonal Antibody)

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STAT1, Polyclonal Antibody (Cat# AAA235821)

Full Name

Phospho-STAT1 (Y701) Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-Stat3 (S727) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-Stat3 (S727) Polyclonal Antibody)

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STAT3, Polyclonal Antibody (Cat# AAA235822)

Full Name

Phospho-STAT3 (S727) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of KB MCF7 cells using Phospho-Stat3 (Y705) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-Stat3 (Y705) Polyclonal Antibody)

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STAT3, Polyclonal Antibody (Cat# AAA235823)

Full Name

Phospho-STAT3 (Y705) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of 293 293T-UV VEC HELA cells using Phospho-Tau (S396) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Tau (S396) Polyclonal Antibody)

Compare (5 max)

MAPT, Polyclonal Antibody (Cat# AAA235824)

Full Name

Phospho-MAPT (S396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of RAT-KIDNEY cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of RAT-kidney cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

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GSK3B, Polyclonal Antibody (Cat# AAA235827)

Full Name

Phospho-GSK3B (S9) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of KB 22RV1 cells using Phospho-KOR-1 (S369) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Rat-kidney cells using Phospho-KOR-1 (S369) Polyclonal Antibody)

Compare (5 max)

OPRK1, Polyclonal Antibody (Cat# AAA235830)

Full Name

Phospho-OPRK1 (S369) Antibody

Gene Names

OPRK1; KOR; OPRK

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Akt1 (S129) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HEPG2 cells using Phospho-Akt1 (S129) Polyclonal Antibody)

Compare (5 max)

AKT1, Polyclonal Antibody (Cat# AAA235832)

Full Name

Phospho-AKT1 (S129) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Smad2/3 (T8) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Smad2/3 (T8) Polyclonal Antibody)

Compare (5 max)

SMAD2/SMAD3, Polyclonal Antibody (Cat# AAA235834)

Full Name

Phospho-SMAD2/SMAD3 (T8) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Akt (T308) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HEPG2 cells using Phospho-Akt (T308) Polyclonal Antibody)

Compare (5 max)

AKT1, Polyclonal Antibody (Cat# AAA235837)

Full Name

Phospho-AKT1 (T308) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of KB MCF7 cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody)

Compare (5 max)

MAPK1/MAPK3, Polyclonal Antibody (Cat# AAA235838)

Full Name

Phospho-MAPK1/MAPK3 (Y222/205) Antibody

Gene Names

MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of RAT-MUSCLE cells using Phospho-Smad2 (S467) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Smad2 (S467) Polyclonal Antibody)

Compare (5 max)

SMAD2, Polyclonal Antibody (Cat# AAA235842)

Full Name

Phospho-SMAD2 (S467) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of MOUSE-INTESTINE cells using Phospho-Lyn (Y508) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Lyn (Y508) Polyclonal Antibody)

Compare (5 max)

LYN, Polyclonal Antibody (Cat# AAA235845)

Full Name

Phospho-LYN (Y508) Antibody

Gene Names

LYN; JTK8; p53Lyn; p56Lyn

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody)

Compare (5 max)

SYK, Polyclonal Antibody (Cat# AAA235846)

Full Name

Phospho-SYK (Y348) Antibody

Gene Names

SYK; p72-Syk

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-FAK (Y407) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-FAK (Y407) Polyclonal Antibody)

Compare (5 max)

PTK2, Polyclonal Antibody (Cat# AAA235848)

Full Name

Phospho-PTK2 (Y407) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293T AD293 cells using Phospho-FAK (Y397) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-FAK (Y397) Polyclonal Antibody)

Compare (5 max)

PTK2, Polyclonal Antibody (Cat# AAA235853)

Full Name

Phospho-PTK2 (Y397) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293T HELA cells using Phospho-Akt1/3 (Y437/434) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-Akt1/3 (Y437/434) Polyclonal Antibody)

Compare (5 max)

AKT1/AKT3, Polyclonal Antibody (Cat# AAA235856)

Full Name

Phospho-AKT1/AKT3 (Y437/434) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-FRS2 (Y436) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS cells using Phospho-FRS2 (Y436) Polyclonal Antibody)

Compare (5 max)

FRS2, Polyclonal Antibody (Cat# AAA235857)

Full Name

Phospho-FRS2 (Y436) Antibody

Gene Names

FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha

Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Polyclonal Antibody)

Compare (5 max)

YWHAZ, Polyclonal Antibody (Cat# AAA235862)

Full Name

Phospho-YWHAZ (S58) Antibody

Gene Names

YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-CREB-1 (S142) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-CREB-1 (S142) Polyclonal Antibody)

Compare (5 max)

CREB1, Polyclonal Antibody (Cat# AAA235863)

Full Name

Phospho-CREB1 (S142) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-HSP90beta (S254) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-HSP90beta (S254) Polyclonal Antibody)

Compare (5 max)

HSP90AB1, Polyclonal Antibody (Cat# AAA235864)

Full Name

Phospho-HSP90AB1 (S254) Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC 293 A549 cells using Phospho-TH (S19) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-TH (S19) Polyclonal Antibody)

Compare (5 max)

TH, Polyclonal Antibody (Cat# AAA235866)

Full Name

Phospho-TH (S19) Antibody

Gene Names

TH; TYH; DYT14; DYT5b

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Ets-1 (T38) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Ets-1 (T38) Polyclonal Antibody)

Compare (5 max)

ETS1, Polyclonal Antibody (Cat# AAA235869)

Full Name

Phospho-ETS1 (T38) Antibody

Gene Names

ETS1; ETS-1; EWSR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of LOVO cells using Phospho-MOR-1 (S375) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Lovo cells using Phospho-MOR-1 (S375) Polyclonal Antibody)

Compare (5 max)

OPRM1, Polyclonal Antibody (Cat# AAA235872)

Full Name

Phospho-OPRM1 (S375) Antibody

Gene Names

OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

WB (Western Blot) (Western Blot analysis of HepG2 cells using Phospho-Nur77 (S351) Polyclonal Antibody)

Compare (5 max)

NR4A1, Polyclonal Antibody (Cat# AAA235682)

Full Name

Phospho-NR4A1 (S351) Antibody

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs