Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of extracts from JK cells untreated or treated with PMA (200nM, 30mins), using PKC theta (Ab-676) antibody (Line 1 and 2) and PKC theta (phospho-Ser676) antibody (Line 3 and 4).)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC theta (phospho-Ser676) antibody.)

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PKC theta, Antibody (Cat# AAA111782)

Full Name

PKC theta (Phospho-Ser676) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with IFN-alpha (1000U/ml, 18 hours), using eIF2alpha (Ab-51) antibody (Line 1 and 2) and eIF2alpha (phospho-Ser51) antibody (Line 3 and 4).)

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eIF2alpha, Antibody (Cat# AAA111783)

Full Name

eIF2alpha (Phospho-Ser51) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Breast Tumor Kinase (Phospho-Tyr447) antibody. Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) antibody.)

Application Data

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Breast Tumor Kinase, Antibody (Cat# AAA111798)

Full Name

Breast Tumor Kinase (Phospho-Tyr447) Antibody

Gene Names

PTK6; BRK

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from Jurkat cells using CHK2 (Phospho-Thr387) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK2 (Phospho-Thr387) Antibody.)

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CHK2, Antibody (Cat# AAA111685)

Full Name

CHK2 (Phospho-Thr387) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Phospho-Ser22+Ser23) antibody. Western blot analysis of extracts from mouse heart cells, using TNNI3 (Phospho-Ser22+Ser23) antibody.)

Application Data (P-peptide-+)

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TNNI3, Antibody (Cat# AAA111704)

Full Name

TNNI3 (Phospho-Ser22+Ser23) Antibody

Gene Names

TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human testis tissue using c-Jun (Phospho-Thr231) antibody. Immunofluorescence analysis of HeLa cells, using c-Jun (Phospho-Thr231) antibody.)

Application Data (P-peptide-+)

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c-Jun, Antibody (Cat# AAA111708)

Full Name

c-Jun (Phospho-Thr231) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) antibody. Western blot analysis of extracts from COS7 cells, using MDM2 (Phospho-Ser166) antibody.)

Application Data (P-peptide-+)

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MDM2, Antibody (Cat# AAA111733)

Full Name

MDM2 (Phospho-Ser166) Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Ku70 (Phospho-Ser5) antibody. Western blot analysis of extracts from HeLa cells, using Ku70 (Phospho-Ser5) antibody.)

Application Data (P-peptide-+)

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Ku70, Antibody (Cat# AAA111562)

Full Name

Ku70 (Phospho-Ser5) Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, using TISB (Phospho-Ser92) antibody.)

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TISB, Antibody (Cat# AAA111575)

Full Name

TISB (Phospho-Ser92) Antibody

Gene Names

ZFP36L1; BRF1; ERF1; cMG1; ERF-1; Berg36; TIS11B; RNF162B

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with TNF-alpha (20ng/ml, 30mins), using NF-kappaB p65 (phospho-Ser311) antibody (Line 1 and 2).)

IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (phospho-Ser311) antibody.)

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NF-kappaB p65, Antibody (Cat# AAA111586)

Full Name

NF-kappaB p65 (Phospho-Ser311) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using CDC16/APC6 (Phospho-Ser560) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC16/APC6 (Phospho-Ser560) antibody. Western blot analysis of extracts from HuvEc cells, using CDC16/APC6 (Phospho-Ser560) antibody.)

Application Data (P-peptide-+)

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CDC16/APC6, Antibody (Cat# AAA111883)

Full Name

CDC16/APC6 (Phospho-Ser560) Antibody

Gene Names

CDC16; APC6; CUT9; ANAPC6; CDC16Hs

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using p47 phox (Phospho-Ser370) antibody. p47 phox (Phospho-Ser370) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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p47 phox, Antibody (Cat# AAA111922)

Full Name

p47 phox (Phospho-Ser370) Antibody

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from K562 cells, using B-Myb (Phospho-Ser577/581) antibody. Immunofluorescence analysis of HeLa cells, t using B-Myb (Phospho-Ser577/581) antibody.)

Application Data

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B-Myb, Antibody (Cat# AAA111923)

Full Name

B-Myb (Phospho-Ser577/581) Antibody

Gene Names

MYBL2; BMYB; B-MYB

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using GRK1 (Phospho-Ser21) antibody. Western blot analysis of extracts from COS7 cells, treated with TNF (20ng/ml, 5mins), using GRK1 (Phospho-Ser21) antibody.)

Application Data (P-peptide-+)

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GRK1, Antibody (Cat# AAA111932)

Full Name

GRK1 (Phospho-Ser21) Antibody

Gene Names

GRK1; RK; RHOK; GPRK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Peptide-+ Western blot analysis of extracts from Jurkat cells, using Lck (Phospho-Tyr393) antibody. Immunofluorescence analysis of HeLa cells, using Lck (Phospho-Tyr393) antibody.)

Application Data

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Lck, Antibody (Cat# AAA111951)

Full Name

Lck (Phospho-Tyr393) Antibody

Gene Names

LCK; LSK; YT16; IMD22; p56lck; pp58lck

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using STAT1 (Phospho-Ser727) antibody. Western blot analysis of extracts from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) antibody.)

Application Data (P-peptide-+)

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STAT1, Antibody (Cat# AAA111855)

Full Name

STAT1 (Phospho-Ser727) Antibody

Gene Names

STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human testis tissue using Rb (Phospho-Thr821) antibody. Immunofluorescence analysis of HeLa cells, using Rb (Phospho-Thr821) antibody.)

Application Data (P-peptide-+)

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Retinoblastoma, Antibody (Cat# AAA111859)

Full Name

Retinoblastoma (Phospho-Thr821) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.)

Application Data (P-peptide-+)

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JAK2, Antibody (Cat# AAA111625)

Full Name

JAK2 (Phospho-Tyr221) Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta tissue using CDC37 (Phospho-Ser13) antibody. Western blot analysis of extracts from NIH/3T3 cells, using CDC37 (Phospho-Ser13) antibody.)

Application Data (P-peptide-+)

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CDC37, Antibody (Cat# AAA111637)

Full Name

CDC37 (Phospho-Ser13) Antibody

Gene Names

CDC37; P50CDC37

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from K562 cells, using Epo-R (Phospho-Tyr368) antibody. Immunofluorescence analysis of HepG2 cells, using Epo-R (Phospho-Tyr368) antibody.)

Application Data

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Epo-R, Antibody (Cat# AAA111663)

Full Name

Epo-R (Phospho-Tyr368) Antibody

Gene Names

EPOR; EPO-R

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human heart tissue using NFAT2 (Phospho-Ser294) antibody.)

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NFAT2, Antibody (Cat# AAA111670)

Full Name

NFAT2 (Phospho-Ser294) Antibody

Gene Names

NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with AAA234321 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

ChIP (Chromatin Immunoprecipitation) (Chromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1B (AAA234321) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysateAll lanes: HIST1H1B antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23 KDaObserved band size: 32 KDa)

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HIST1H1B, Polyclonal Antibody (Cat# AAA234321)

Full Name

Phospho-HIST1H1B (T154) Antibody

Gene Names

HIST1H1B; H1; H1B; H1.5; H1F5; H1s-3

Reactivity

Human

Applications

Chromatin immunoprecipitation, Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemistry analysis of HepG2 cells using AAA234323 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in: MCF-7 whole cell lysateAll lanes: HIST1H1B antibody at 1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23 KDaObserved band size: 32 KDa)

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HIST1H1B, Polyclonal Antibody (Cat# AAA234323)

Full Name

Phospho-HIST1H1B (S17) Antibody

Gene Names

HIST1H1B; H1; H1B; H1.5; H1F5; H1s-3

Reactivity

Human

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA234326 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

WB (Western Blot) (Western BlotPositive WB detected in: Rat kidney tissueAll lanes: HIST1H1B antibody at 1ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23 KDaObserved band size: 32 KDa)

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HIST1H1B, Polyclonal Antibody (Cat# AAA234326)

Full Name

Phospho-HIST1H1B (S188) Antibody

Gene Names

HIST1H1B; H1; H1B; H1.5; H1F5; H1s-3

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using AAA234341 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

ICC (Immunocytochemistry) (Immunocytochemistry analysis of Hela cells using AAA234341 at dilution of 1:100)

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HIST1H1E, Polyclonal Antibody (Cat# AAA234341)

Full Name

Phospho-HIST1H1E (S35) Antibody

Gene Names

HIST1H1E; H1E; H1.4; H1F4; RMNS; H1s-4; dJ221C16.5

Reactivity

Human

Applications

Immunofluorescence

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of hela with AAA234373, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Mouse spleen tissueAll lanes: HIST1H1E antibody at 1.86ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 22 KDaObserved band size: 22 KDa)

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HIST1H1E, Polyclonal Antibody (Cat# AAA234373)

Full Name

Phospho-HIST1H1E (T17) Antibody

Gene Names

HIST1H1E; H1E; H1.4; H1F4; RMNS; H1s-4; dJ221C16.5

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of hela with AAA234374, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateAll lanes: H2AFX antibody at 1.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 16 KDaObserved band size: 16 KDa)

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H2AFX, Polyclonal Antibody (Cat# AAA234374)

Full Name

Phospho-H2AFX (S139) Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human

Applications

Chromatin immunoprecipitation, Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$240/0.05 mL $360/0.1 mL $695/2x0.1 mL $1,010/3x0.1 mL $1,315/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Immunofluorescence staining of MCF-7 cells with AAA235171 at 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of AAA235171 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA235171 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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Histone H1.4, Monoclonal Recombinant Antibody (Cat# AAA235171)

Full Name

Phospho-Histone H1.4 (T17) Antibody

Gene Names

HIST1H1E; H1E; H1.4; H1F4; RMNS; H1s-4; dJ221C16.5

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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Application Data (Overlay histogram showing Hela cells stained with AAA235162 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)

ICC (Immunocytochemistry) (Immunocytochemistry analysis of AAA235162 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysateAll lanes: Phospho-Histone H3 (T3) antibody at 1.41ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 16 KDaObserved band size: 16 KDa)

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Histone H3.3, Monoclonal Recombinant Antibody (Cat# AAA235162)

Full Name

Phospho-Histone H3.3 (T3) Antibody

Gene Names

H3F3A; H3F3; H3.3A

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image of CSB-RA010833A82phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-HSPB1 antibody at 0.73ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 27 KDaObserved band size: 27 KDa)

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HSPB1, Monoclonal Recombinant Antibody (Cat# AAA235562)

Full Name

Phospho-HSPB1 (S82) Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG (1ug) instead of AAA235567 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA235567 (3ug)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20ug))

IHC (Immunohistochemisry) (IHC image of AAA235567 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry) (IHC image of AAA235567 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in Hela whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not)All lanes Phospho-JAK2 antibody at 0.75ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120 KDaObserved band size: 120 KDa)

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JAK2, Monoclonal Recombinant Antibody (Cat# AAA235567)

Full Name

Phospho-JAK2 (Y1007 + Y1008) Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-LAT in A549 whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA012767A191phHU in A549 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA012767A191phHU(3ug)+ A549 whole cell lysate(1mg)Lane 3: A549 whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemisry) (IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry) (IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Jurkat whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)All lanes?Phospho-LAT antibody at 2.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

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LAT, Monoclonal Recombinant Antibody (Cat# AAA235569)

Full Name

Phospho-LAT (Y191) Antibody

Gene Names

LAT; LAT1; pp36; IMD52

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA013456A185phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA013456A185phHU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate,HepG2 whole cell lysate(treated with EGF or not)All lanes?Phospho-MAPK3 antibody at 2.35ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDa)

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MAPK3, Monoclonal Recombinant Antibody (Cat# AAA235571)

Full Name

Phospho-MAPK3 (T202) + MAPK1 (T185) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with EGF or not)All lanes?Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46,54 KDaObserved band size: 46,54 KDa)

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MAPK8/MAPK9/MAPK10, Monoclonal Recombinant Antibody (Cat# AAA235572)

Full Name

Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with CSB-RA015761A32phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-NFKBIA antibody at 1.07ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39 KDaObserved band size: 39 KDa)

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NFKBIA, Monoclonal Recombinant Antibody (Cat# AAA235576)

Full Name

Phospho-NFKBIA (S32) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI; EDAID2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-PAK4/PAK5/PAK6 antibody at 2.15ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,75,80 KDaObserved band size: 75,80 KDa)

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PAK4/PAK5/PAK6, Monoclonal Recombinant Antibody (Cat# AAA235578)

Full Name

Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Antibody

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA018327A05phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

ICC (Immunocytochemistry) (Immunocytochemistry analysis of CSB-RA018327A05phHU diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysateAll lanes?Phospho-POLR2A antibody at 0.75ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 270 KDaObserved band size: 270 KDa)

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POLR2A, Monoclonal Recombinant Antibody (Cat# AAA235580)

Full Name

Phospho-POLR2A (S5) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA019284A621phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-RAF1 antibody at 1.525ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73 KDaObserved band size: 73 KDa)

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RAF1, Monoclonal Recombinant Antibody (Cat# AAA235585)

Full Name

Phospho-RAF1 (S621) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-RB1 in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA019386A780phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA019386A780phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of K562 cells with CSB-RA019386A780phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of CSB-RA019386A780phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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RB1, Monoclonal Recombinant Antibody (Cat# AAA235586)

Full Name

Phospho-RB1 (S780) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunohistochemistry

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image of CSB-RA022812A705phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate,Jurkat whole cell lysate(treated with EGF or Pervanadate)All lanes?Phospho-STAT3 antibody at 0.925ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88 KDaObserved band size: 88 KDa)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA235591)

Full Name

Phospho-STAT3 (Tyr705) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of 293 cells(treated with 50mM Calyculin A for 30min) with CSB-RA024077A55phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with Calyculin A or Pervanadate)All lanes?Phospho-TP53 antibody at 1.28ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 KDaObserved band size: 53 KDa)

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TP53, Monoclonal Recombinant Antibody (Cat# AAA235596)

Full Name

Phospho-TP53 (T55) Antibody

Gene Names

TP53; P53; BCC7; LFS1; BMFS5; TRP53

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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WB (Western Blot) (Western blot analysis of customer's lysis using Phospho-mTOR (S2448) antibody.)

WB (Western Blot) (Western Blot analysis of HY926 cells using Phospho-mTOR (S2448) Polyclonal Antibody)

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MTOR, Polyclonal Antibody (Cat# AAA235812)

Full Name

Phospho-MTOR (S2448) Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-NFkappaB-p65 (S536) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-NFkappaB-p65 (S536) Polyclonal Antibody)

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RELA, Polyclonal Antibody (Cat# AAA235814)

Full Name

Phospho-RELA (S536) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of customer's using Phospho-p53 (T18) Polyclonal Antibody.)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-p53 (T18) Polyclonal Antibody)

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TP53, Polyclonal Antibody (Cat# AAA235816)

Full Name

Phospho-TP53 (T18) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of HELA PC3 MCF7 22RV1 cells using Phospho-PKC zeta (T410) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 823 cells using Phospho-PKC zeta (T410) Polyclonal Antibody)

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PRKCZ, Polyclonal Antibody (Cat# AAA235819)

Full Name

Phospho-PRKCZ (T410) Antibody

Gene Names

PRKCZ; PKC2; PKC-ZETA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of SH-SY5Y MOUSE-BRAIN cells using Phospho-Stat1 (Y701) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-Stat1 (Y701) Polyclonal Antibody)

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STAT1, Polyclonal Antibody (Cat# AAA235821)

Full Name

Phospho-STAT1 (Y701) Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-Stat3 (S727) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-Stat3 (S727) Polyclonal Antibody)

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STAT3, Polyclonal Antibody (Cat# AAA235822)

Full Name

Phospho-STAT3 (S727) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of KB MCF7 cells using Phospho-Stat3 (Y705) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-Stat3 (Y705) Polyclonal Antibody)

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STAT3, Polyclonal Antibody (Cat# AAA235823)

Full Name

Phospho-STAT3 (Y705) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of 293 293T-UV VEC HELA cells using Phospho-Tau (S396) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Tau (S396) Polyclonal Antibody)

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MAPT, Polyclonal Antibody (Cat# AAA235824)

Full Name

Phospho-MAPT (S396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of RAT-KIDNEY cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of RAT-kidney cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

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GSK3B, Polyclonal Antibody (Cat# AAA235827)

Full Name

Phospho-GSK3B (S9) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs