Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p65 (phospho-Ser311) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B-p65 (phospho-Ser311) antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with IFN-α using NFκB-p65((phospho-Ser311) Antibody.)

Compare (5 max)

RELA, Polyclonal Antibody (Cat# AAA243340)

Full Name

Phospho-RELA (Ser311) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKalpha1 (Phospho-Ser487) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1(Phospho-Ser487)Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Phospho-Ser487)Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKa1(Phospho-Ser487)Antibody.)

Compare (5 max)

PRKAA1/PRKAA2, Polyclonal Antibody (Cat# AAA243365)

Full Name

Phospho-PRKAA1/PRKAA2 (Ser487) Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody. The lane on the right is treated with the synthesized peptide.)

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BIK, Polyclonal Antibody (Cat# AAA243242)

Full Name

Phospho-BIK (Thr33) Antibody

Gene Names

BIK; BP4; NBK; BIP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody. The lane on the right is treated with the synthesized peptide.)

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STAT5B, Polyclonal Antibody (Cat# AAA243250)

Full Name

Phospho-STAT5B (Ser731) Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK alpha (Phospho-Thr23) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody.)

Compare (5 max)

CHUK, Polyclonal Antibody (Cat# AAA243282)

Full Name

Phospho-CHUK (Thr23) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibody.)

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GRIN2B, Polyclonal Antibody (Cat# AAA243102)

Full Name

Phospho-GRIN2B (Tyr1474) Antibody

Gene Names

GRIN2B; MRD6; NR2B; hNR3; GluN2B; NMDAR2B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Phospho-Tyr753) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody.)

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PLCG2, Polyclonal Antibody (Cat# AAA243104)

Full Name

Phospho-PLCG2 (Tyr753) Antibody

Gene Names

PLCG2; FCAS3; APLAID

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Phospho-Ser639) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum using IRS-1(Phospho-Ser639) Antibody.)

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IRS1, Polyclonal Antibody (Cat# AAA243126)

Full Name

Phospho-IRS1 (Ser639) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1(Phospho-Ser1423) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using BRCA1(Phospho-Ser1423) Antibody.)

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BRCA1, Polyclonal Antibody (Cat# AAA243128)

Full Name

Phospho-BRCA1 (Ser1423) Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3(Phospho-Ser425) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using Smad3(Phospho-Ser425) Antibody.)

Compare (5 max)

SMAD3, Polyclonal Antibody (Cat# AAA243151)

Full Name

Phospho-SMAD3 (Ser425) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Phospho-Tyr936) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from C6 cell untreated or treated with serum usingc-kit(phospho-Tyr936) Antibody.)

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KIT, Polyclonal Antibody (Cat# AAA243161)

Full Name

Phospho-KIT (Tyr936) Antibody

Gene Names

KIT; PBT; SCFR; C-Kit; CD117

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(phospho-Tyr42) antibody.)

WB (Western Blot) (Western blot analysis of extract from Hela cells untreated or treated with serum using IkB-a(Phospho-Tyr42) antibody.)

Compare (5 max)

NFKBIA, Polyclonal Antibody (Cat# AAA243164)

Full Name

Phospho-NFKBIA (Tyr42) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105 (phospho-Ser893) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p105 (phospho-Ser893) antibody.)

Compare (5 max)

NFKB1, Polyclonal Antibody (Cat# AAA243171)

Full Name

Phospho-NFKB1 (Ser893) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the left is treated with antigen-specific peptide.)

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RB1, Polyclonal Antibody (Cat# AAA243204)

Full Name

Phospho-RB1 (Ser608) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemisry) (Immunofluorescence staining of Hela cells with AAA235168 at 1:31, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

ICC (Immunocytochemistry) (Immunocytochemistry analysis of AAA235168 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysateAll lanes: Phospho-Histone H3.1 (S10)antibody at 0.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)

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Histone H3.1, Monoclonal Recombinant Antibody (Cat# AAA235168)

Full Name

Phospho-Histone H3.1 (S10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

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UBF1, Monoclonal Antibody (Cat# AAA128174)

Full Name

Anti-Phospho-UBF1 (S484) Rabbit Monoclonal Antibody

Gene Names

UBTF; UBF; UBF1; UBF-1; NOR-90

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.)

WB (Western Blot) (Western blot analysis of Phospho-P70 S6 Kinase beta (S371) expression in 293 cell lysate.)

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P70 S6 Kinase beta, Monoclonal Antibody (Cat# AAA128176)

Full Name

Anti-Phospho-P70 S6 Kinase beta (S371) Rabbit Monoclonal Antibody

Gene Names

RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells, using ADCY1 antibody.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

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PKA C, Monoclonal Antibody (Cat# AAA128126)

Full Name

Anti-Phospho-PKA C (Thr197) Rabbit Monoclonal Antibody

Gene Names

PRKACG; KAPG; PKACg

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.)

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Phospholamban, Monoclonal Antibody (Cat# AAA128135)

Full Name

Anti-Phospho-Phospholamban (S16) Rabbit Monoclonal Antibody

Gene Names

PLN; PLB; CMD1P; CMH18

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYPT1 (Phospho-Thr696) antibody. Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) antibody.)

Application Data (P-peptide-+)

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MYPT1, Antibody (Cat# AAA112116)

Full Name

MYPT1 (Phospho-Thr696) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (FITC) $580/0.1 mL (AF350) $580/0.1 mL (AF488) $580/0.1 mL (AF555) $580/0.1 mL (AF568) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells using STAT3 (phospho-Ser727) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (phospho-Ser727) antibody.)

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STAT3, Antibody (Cat# AAA112118)

Full Name

STAT3 (Phospho-Ser727) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using AurA (Phospho-Ser342) antibody. AurA (Phospho-Ser342) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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AurA, Antibody (Cat# AAA112126)

Full Name

AurA (Phospho-Ser342) Antibody

Gene Names

AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from COLO205 cells, treated with Serum (20%, 15mins), using JIP1 (Phospho-Thr103) antibody. Immunofluorescence analysis of NIH/3T3 cells, using JIP1 (Phospho-Thr103) antibody.)

Application Data

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JIP1, Antibody (Cat# AAA112154)

Full Name

JIP1 (Phospho-Thr103) Antibody

Gene Names

MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK beta1 (Phospho-Ser181) antibody. Western blot analysis of extracts from COS7 cells treated with PMA (125ng/ml, 30mins), using AMPK beta1 (Phospho-Ser181) antibody.)

Application Data (P-peptide-+)

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AMPK beta1, Antibody (Cat# AAA111477)

Full Name

AMPK beta1 (Phospho-Ser181) Antibody

Gene Names

PRKAB1; AMPK; HAMPKb

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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WB (Western Blot) (Western blot analysis of extracts from K562 cells, using Met (phospho-Tyr1349) antibody. Western blot analysis of extracts from HepG2 cells, using Met (phospho-Tyr1349) antibody.)

Application Data

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Met, Antibody (Cat# AAA111478)

Full Name

Met (Phospho-Tyr1349) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PAK2 (Phospho-Ser20) antibody. Western blot analysis of extracts from 293 cells, treated with Sorbitol (0.4M, 30mins), using PAK2 (Phospho-Ser20) antibody.)

Application Data (P-peptide-+)

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PAK2, Antibody (Cat# AAA111486)

Full Name

PAK2 (Phospho-Ser20) Antibody

Gene Names

PAK2; PAK65; PAKgamma

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HepG2 cells, using c-Met (Phospho-Tyr1003) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using c-Met (Phospho-Tyr1003) antibody. Western blot analysis of extracts from HepG2 cells, using c-Met (Phospho-Tyr1003) antibody.)

Application Data (P-peptide-+)

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c-Met, Antibody (Cat# AAA111487)

Full Name

c-Met (Phospho-Tyr1003) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Line 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Line 3 and 4).)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.)

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4E-BP1, Antibody (Cat# AAA112080)

Full Name

4E-BP1 (Phospho-Thr36) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ERK3 (Phospho-Ser189) antibody. Western blot analysis of extracts from mouse brain, using ERK3 (Phospho-Ser189) antibody.)

Application Data (P-peptide-+)

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ERK3, Antibody (Cat# AAA111461)

Full Name

ERK3 (Phospho-Ser189) Antibody

Gene Names

MAPK6; ERK3; PRKM6; p97MAPK; HsT17250

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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Application Data (P-peptide-+)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain carcinoma tissue using CXCR4 (Phospho-Ser339) antibody. Western blot analysis of extracts from HUVEC cells, treated with etoposide (25uM, 24hours), using CXCR4 (Phospho-Ser339) antibody.)

Application Data (P-peptide-+)

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CXCR4, Antibody (Cat# AAA112193)

Full Name

CXCR4 (Phospho-Ser339) Antibody

Gene Names

CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells using Histone H3.1 (phospho-Ser10) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (phospho-Ser10) antibody.)

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Histone H3.1, Antibody (Cat# AAA112196)

Full Name

Histone H3.1 (Phospho-Ser10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from 293 cells (10% serum-treated, 15min) and HeLa cells (EGF-treated, 200ng/ml, 15min), using FKHR (Ab-319) antibody (Line 1, 2 and 3) and FKHR (phospho-Ser319) antibody (Line 4 and 5).)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (phospho-Ser319) antibody.)

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FKHR, Antibody (Cat# AAA112224)

Full Name

FKHR (Phospho-Ser319) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using PKD1/PKC mu (Phospho-Ser205) antibody. Western blot analysis of extracts from NIH/3T3 cells treated with Anisomycin (25mug/ml, 30mins) (lane 1) and NIH/3T3 cells treated with H2O2 (100muM, 30mins) (lane 2), using PKD1/PKC mu (Phospho-Ser205) antibody.)

Application Data (P-peptide-+)

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PKD1/PKC, Antibody (Cat# AAA112236)

Full Name

PKD1/PKC mu (Phospho-Ser205) Antibody

Gene Names

PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (Immunofluorescence analysis of Human lung tissue with Phospho-PPP1R12A (Thr853) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-PPP1R12A (Thr853) Polyclonal Antibody at dilution of 1:2000)

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MYPT1, Polyclonal Antibody (Cat# AAA172568)

Full Name

Phospho-MYPT1 (T853) Polyclonal Antibody

Gene Names

Ppp1r12a; Mypt1; AA792106; AV099298; D10Ertd625e; 1200015F06Rik; 5730577I22Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-Smad3 (Ser425) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse lung tissue with Phospho-Smad3 (Ser425) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Smad3 (Ser425) Polyclonal Antibody at dilution of 1:500)

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Smad3, Polyclonal Antibody (Cat# AAA172574)

Full Name

Phospho-Smad3 (S425) Polyclonal Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 3T3 cells with Phospho-Met (Tyr1356) Polyclonal Antibody)

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Met, Polyclonal Antibody (Cat# AAA172588)

Full Name

Phospho-Met (Y1356) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse liver tissue with Phospho-JNK1/2/3 (Thr183/Y185) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus tissue with Phospho-JNK1/2/3 (Thr183/Y185) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-JNK1/2/3 (Thr183/Y185) Polyclonal Antibody at dilution of 1:2000)

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JNK1/2/3, Polyclonal Antibody (Cat# AAA172608)

Full Name

Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 3T3 cells with Phospho-HDAC6 (Ser22) Polyclonal Antibody at dilution of 1:500)

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HDAC6, Polyclonal Antibody (Cat# AAA172376)

Full Name

Phospho-HDAC6 (S22) Polyclonal Antibody

Gene Names

HDAC6; HD6; JM21; CPBHM; PPP1R90

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-Stat1 (Tyr701) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat lung tissue with Phospho-Stat1 (Tyr701) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of COS-7 cells with Phospho-Stat1 (Tyr701) Polyclonal Antibody at dilution of 1:1000)

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Stat1, Polyclonal Antibody (Cat# AAA172394)

Full Name

Phospho-Stat1 (Y701) Polyclonal Antibody

Gene Names

Stat1; AA408197; 2010005J02Rik

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of COS7 cells using Phospho-IGF-IR (Tyr1165/Y1166) Polyclonal Antibody at dilution of 1:500.)

WB (Western Blot) (Western Blot analysis of K562 cells using Phospho-IGF-IR (Tyr1165/Y1166) Polyclonal Antibody at dilution of 1:500.)

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IGF-IR, Polyclonal Antibody (Cat# AAA172407)

Full Name

Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of Hela cells with Phospho-PKD2 (Ser876) Polyclonal Antibody at dilution of 1:1000)

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PKD2, Polyclonal Antibody (Cat# AAA172432)

Full Name

Phospho-PKD2 (S876) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus tissue with Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of Hela cells using Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:500)

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Chk2, Polyclonal Antibody (Cat# AAA172461)

Full Name

Phospho-Chk2 (T68) Polyclonal Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-ERK 1/2 (Tyr222/205) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human appendix tissue with Phospho-ERK 1/2 (Tyr222/205) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of KB cells with Phospho-ERK 1/2 (Tyr222/205) Polyclonal Antibody at dilution of 1:500)

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ERK 1/2, Polyclonal Antibody (Cat# AAA171643)

Full Name

Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody at dilution of 1:50)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody)

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ERK 1/2, Polyclonal Antibody (Cat# AAA171662)

Full Name

Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-I?B-? (Ser32/S36) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney tissue with Phospho-I?B-? (Ser32/S36) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of COS-7 cells with Phospho-I?B-? (Ser32/S36) Polyclonal Antibody)

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IkappaB-alpha, Polyclonal Antibody (Cat# AAA171690)

Full Name

Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-p38 (Thr180) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat lung tissue with Phospho-p38 (Thr180) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-p38 (Thr180) Polyclonal Antibody)

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p38, Polyclonal Antibody (Cat# AAA172001)

Full Name

Phospho-p38 (T180) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunofluorescence, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Stat6 (Tyr641) Polyclonal Antibody)

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Stat6, Polyclonal Antibody (Cat# AAA171800)

Full Name

Phospho-Stat6 (Y641) Polyclonal Antibody

Gene Names

STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 3T3 cells with Phospho-MerTK/Tyro3 (Tyr749/681) Polyclonal Antibody at dilution of 1:500)

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MerTK/Tyro3, Polyclonal Antibody (Cat# AAA171877)

Full Name

Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody

Gene Names

MERTK; MER; RP38; c-Eyk; c-mer; Tyro12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:1000)

Compare (5 max)

mTOR, Polyclonal Antibody (Cat# AAA172193)

Full Name

Phospho-mTOR (S2448) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-KIF20A (Ser528) Polyclonal Antibody)

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KIF20A, Polyclonal Antibody (Cat# AAA172206)

Full Name

Phospho-KIF20A (S528) Polyclonal Antibody

Gene Names

KIF20A; MKLP2; RAB6KIFL

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs