Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western Blot analysis of HELA A431 cells using Phospho-EphB1/2 (Y594/604) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Hela cells using Phospho-EphB1/2 (Y594/604) Polyclonal Antibody)

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EPHB1/EPHB2, Polyclonal Antibody (Cat# AAA235840)

Full Name

Phospho-EPHB1/EPHB2 (Y594/604) Antibody

Gene Names

EPHB1; ELK; NET; Hek6; EPHT2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-p63 (S455) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-p63 (S455) Polyclonal Antibody)

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TP63, Polyclonal Antibody (Cat# AAA235849)

Full Name

Phospho-TP63 (S455) Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of MCF7 PC-3 HELA KB cells using Phospho-YAP (S127) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of MCF7 cells using Phospho-YAP (S127) Polyclonal Antibody)

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YAP1, Polyclonal Antibody (Cat# AAA235851)

Full Name

Phospho-YAP1 (S127) Antibody

Gene Names

YAP1; YAP; YKI; YAP2; YAP65

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-Smad3 (S208) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-Smad3 (S208) Polyclonal Antibody)

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SMAD3, Polyclonal Antibody (Cat# AAA235854)

Full Name

Phospho-SMAD3 (S208) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody)

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DUSP1/DUSP4, Polyclonal Antibody (Cat# AAA235858)

Full Name

Phospho-DUSP1/DUSP4 (S296/318) Antibody

Gene Names

DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-PKC delta (S645) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HuvEc cells using Phospho-PKC delta (S645) Polyclonal Antibody)

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PRKCD, Polyclonal Antibody (Cat# AAA235861)

Full Name

Phospho-PRKCD (S645) Antibody

Gene Names

PRKCD; MAY1; PKCD; nPKC-delta

Reactivity

Human, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of 293T AD293 22RV1 HELA cells using Phospho-c-Myc (S62) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-c-Myc (S62) Polyclonal Antibody)

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MYC, Polyclonal Antibody (Cat# AAA235865)

Full Name

Phospho-MYC (S62) Antibody

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-Caldesmon (S789) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Caldesmon (S789) Polyclonal Antibody)

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CALD1, Polyclonal Antibody (Cat# AAA235868)

Full Name

Phospho-CALD1 (S789) Antibody

Gene Names

CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IHC (Immunohiostchemistry) (IHC image of CSB-RA017407A144phHU diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate(treated with EGF or not)All lanes?Phospho-PAK1/PAK2/PAK3 antibody at 1.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65 KDaObserved band size: 65 KDa)

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PAK1/PAK2/PAK3, Monoclonal Recombinant Antibody (Cat# AAA235577)

Full Name

Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody

Gene Names

PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-POLR2A in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA018327A02phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA018327A02phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA018327A02phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA018327A02phHU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysateAll lanes?Phospho-POLR2A antibody at 1.02ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 270 KDaObserved band size: 270 KDa)

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POLR2A, Monoclonal Recombinant Antibody (Cat# AAA235579)

Full Name

Phospho-POLR2A (S2) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with CSB-RA018699A638phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,A549 whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-PRKCA antibody at 0.68ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 80 KDaObserved band size: 80 KDa)

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PRKCA, Monoclonal Recombinant Antibody (Cat# AAA235582)

Full Name

Phospho-PRKCA (T638) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG(1ug)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA019025A542phHU(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20ug))

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)All lanes?Phospho-PTPN11 antibody at 0.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68 KDaObserved band size: 68 KDa)

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PTPN11, Monoclonal Recombinant Antibody (Cat# AAA235583)

Full Name

Phospho-PTPN11 (Y542) Antibody

Gene Names

PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-RPS6KB1 in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA020470A421phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA020470A421phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA020470A421phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-RPS6KB1 antibody at 0.93ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 70 KDaObserved band size: 70 KDa)

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RPS6KB1, Monoclonal Recombinant Antibody (Cat# AAA235587)

Full Name

Phospho-RPS6KB1 (T421+S424) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with CSB-RA021912A129phHU at 1:206,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of CSB-RA021912A129phHU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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SNCA, Monoclonal Recombinant Antibody (Cat# AAA235588)

Full Name

Phospho-SNCA (S129) Antibody

Gene Names

SNCA; PD1; NACP; PARK1; PARK4

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells(treated with 100mM Calyculin A for 30min) with CSB-RA022810A727phHU at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA022810A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?HepG2 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-STAT1 antibody at 1.065ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 87 KDaObserved band size: 87 KDa)

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STAT1, Monoclonal Recombinant Antibody (Cat# AAA235590)

Full Name

Phospho-STAT1 (S727) Antibody

Gene Names

STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA024077A33phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of CSB-RA024077A33phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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TP53, Monoclonal Recombinant Antibody (Cat# AAA235595)

Full Name

Phospho-TP53 (S33) Antibody

Gene Names

TP53; P53; BCC7; LFS1; BMFS5; TRP53

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA618984A380phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA618984A380phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysateAll lanes?Phospho-RPS6KA1 antibody at 1.75ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

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RPS6KA1, Monoclonal Recombinant Antibody (Cat# AAA235604)

Full Name

Phospho-RPS6KA1 (S380) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with CSB-RA007795A724phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate,A549 whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-PRKAA2 antibody at 1.95ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 KDaObserved band size: 62 KDa)

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PRKAA2, Monoclonal Recombinant Antibody (Cat# AAA235606)

Full Name

Phospho-PRKAA2 (Thr172) Antibody

Gene Names

PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-MEF-2 (S408) Polyclonal Antibody)

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MEF2A, Polyclonal Antibody (Cat# AAA235652)

Full Name

Phospho-MEF2A (S408) Antibody

Gene Names

MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of L929 RAT-KIDNEY MOUSE-BRAIN cells using Phospho-AMPKalpha1/2 (T183/172) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of KB cells using Phospho-AMPKalpha1/2 (T183/172) Polyclonal Antibody)

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PRKAA1/PRKAA2, Polyclonal Antibody (Cat# AAA236704)

Full Name

Phospho-PRKAA1/PRKAA2 (T183/172) Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HEPG2 KB using Phospho-Akt1 (S246) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of NIH-3T3 K562 cells using Phospho-Akt1 (S246) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-Akt1 (S246) Polyclonal Antibody)

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AKT1, Polyclonal Antibody (Cat# AAA236712)

Full Name

Phospho-AKT1 (S246) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of KB using Phospho-Akt1 (Y474) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T 22RV1 cells using Phospho-Akt1 (Y474) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-Akt1 (Y474) Polyclonal Antibody)

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AKT1, Polyclonal Antibody (Cat# AAA236718)

Full Name

Phospho-AKT1 (Y474) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-Bcl-2 (S70) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-Bcl-2 (S70) Polyclonal Antibody)

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BCL2, Polyclonal Antibody (Cat# AAA236577)

Full Name

Phospho-BCL2 (S70) Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

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WB (Western Blot) (Western Blot analysis of 22RV1 HELA cells using Phospho-p16 (S326) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of JK cells using Phospho-p16 (S326) Polyclonal Antibody)

Compare (5 max)

CDKN2A, Polyclonal Antibody (Cat# AAA236582)

Full Name

Phospho-CDKN2A (S326) Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293T AD293 22RV1 HELA cells using Phospho-MYPT1 (T853) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293T cells using Phospho-MYPT1 (T853) Polyclonal Antibody)

Compare (5 max)

PPP1R12A, Polyclonal Antibody (Cat# AAA236612)

Full Name

Phospho-PPP1R12A (T853) Antibody

Gene Names

PPP1R12A; MBS; M130; MYPT1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of customer's lysis using Phospho-FAK (S843) antibody.)

WB (Western Blot) (Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-FAK (S843) antibody.)

Compare (5 max)

PTK2, Polyclonal Antibody (Cat# AAA236619)

Full Name

Phospho-PTK2 (S843) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

Standard Curve (Sample)

Compare (5 max)

p-tau202 (Phospho Tau 202), ELISA Kit (Cat# AAA251148)

Full Name

Human p-tau202 (Phospho Tau 202) ELISA Kit

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

Compare (5 max)

p-tau231 (Phospho Tau 231), ELISA Kit (Cat# AAA251042)

Full Name

Human p-tau231 (Phospho Tau 231) ELISA Kit

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

< >

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Phospho-Ser259) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 and MDA cells untreated or treated with PMA using Raf1(Phospho-Ser259) Antibody.)

Compare (5 max)

RAF1, Polyclonal Antibody (Cat# AAA243041)

Full Name

Phospho-RAF1 (Ser259) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1(Phospho-Ser389) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using Elk1(Phospho-Ser389) Antibody.)

Compare (5 max)

ELK1, Polyclonal Antibody (Cat# AAA243053)

Full Name

Phospho-ELK1 (Ser389) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1(Phospho-Tyr14) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with H2O2(lane 2) using Caveolin-1(Phospho-Tyr14) Antibody.)

Compare (5 max)

CAV1, Polyclonal Antibody (Cat# AAA243074)

Full Name

Phospho-CAV1 (Tyr14) Antibody

Gene Names

CAV1; CGL3; BSCL3; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser37) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53(Phospho-Ser37) Antibody.)

Compare (5 max)

TP53, Polyclonal Antibody (Cat# AAA243077)

Full Name

Phospho-TP53 (Ser37) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser396) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

MAPT, Polyclonal Antibody (Cat# AAA243079)

Full Name

Phospho-MAPT (Ser396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using HDAC8(Phospho-Ser39) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Compare (5 max)

HDAC8, Polyclonal Antibody (Cat# AAA243089)

Full Name

Phospho-HDAC8 (Ser39) Antibody

Gene Names

HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser807) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody.)

Compare (5 max)

RB1, Polyclonal Antibody (Cat# AAA243090)

Full Name

Phospho-RB1 (Ser807) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibody.)

Compare (5 max)

GRIN2B, Polyclonal Antibody (Cat# AAA243102)

Full Name

Phospho-GRIN2B (Tyr1474) Antibody

Gene Names

GRIN2B; MRD6; NR2B; hNR3; GluN2B; NMDAR2B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Phospho-Tyr753) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody.)

Compare (5 max)

PLCG2, Polyclonal Antibody (Cat# AAA243104)

Full Name

Phospho-PLCG2 (Tyr753) Antibody

Gene Names

PLCG2; FCAS3; APLAID

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Ser15) Antibody.)

Compare (5 max)

MYL9, Polyclonal Antibody (Cat# AAA243038)

Full Name

Phospho-MYL9 (Ser15) Antibody

Gene Names

MYL9; LC20; MLC2; MRLC1; MYRL2; MLC-2C

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6(Phospho-Tyr24) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with starvation(lane 2) using CDK6(phospho-Tyr24) Antibody.)

Compare (5 max)

CDK6, Polyclonal Antibody (Cat# AAA243328)

Full Name

Phospho-CDK6 (Tyr24) Antibody

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extract from MDA-MB-435 cells untreated or treated with TNF-alpha; (20ng/ml, 5min) using NF-κB p100(phospho-Ser870) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p100(phospho- Ser870) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p100(phospho-Ser870) antibody.)

Compare (5 max)

NFKB2, Polyclonal Antibody (Cat# AAA243335)

Full Name

Phospho-NFKB2 (Ser870) Antibody

Gene Names

NFKB2; p52; p105; H2TF1; LYT10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (Lane 3 and 4).)

Compare (5 max)

FOXO3, Polyclonal Antibody (Cat# AAA243339)

Full Name

Phospho-FOXO3 (Ser253) Antibody

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p65 (phospho-Ser311) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B-p65 (phospho-Ser311) antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with IFN-α using NFκB-p65((phospho-Ser311) Antibody.)

Compare (5 max)

RELA, Polyclonal Antibody (Cat# AAA243340)

Full Name

Phospho-RELA (Ser311) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKalpha1 (Phospho-Ser487) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1(Phospho-Ser487)Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Phospho-Ser487)Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKa1(Phospho-Ser487)Antibody.)

Compare (5 max)

PRKAA1/PRKAA2, Polyclonal Antibody (Cat# AAA243365)

Full Name

Phospho-PRKAA1/PRKAA2 (Ser487) Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

BIK, Polyclonal Antibody (Cat# AAA243242)

Full Name

Phospho-BIK (Thr33) Antibody

Gene Names

BIK; BP4; NBK; BIP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

STAT5B, Polyclonal Antibody (Cat# AAA243250)

Full Name

Phospho-STAT5B (Ser731) Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK alpha (Phospho-Thr23) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody.)

Compare (5 max)

CHUK, Polyclonal Antibody (Cat# AAA243282)

Full Name

Phospho-CHUK (Thr23) Antibody

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

ADD1, Polyclonal Antibody (Cat# AAA243296)

Full Name

Phospho-ADD1 (Ser726) Antibody

Gene Names

ADD1; ADDA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Phospho-Ser303) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF7 cells using HSF1(phospho-Ser303) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

Compare (5 max)

HSF1, Polyclonal Antibody (Cat# AAA243310)

Full Name

Phospho-HSF1 (Ser303) Antibody

Gene Names

HSF1; HSTF1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Phospho-Ser411) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser411) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser411) Antibody.)

Compare (5 max)

RPS6KB1, Polyclonal Antibody (Cat# AAA243313)

Full Name

Phospho-RPS6KB1 (Ser411) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(phospho-Tyr42) antibody.)

WB (Western Blot) (Western blot analysis of extract from Hela cells untreated or treated with serum using IkB-a(Phospho-Tyr42) antibody.)

Compare (5 max)

NFKBIA, Polyclonal Antibody (Cat# AAA243164)

Full Name

Phospho-NFKBIA (Tyr42) Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs