Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.
WB (Western Blot) (Western blot analysis of extracts from HeLa cells, treated with TSA (400nM, 24hours), using EGFR (Phospho-Ser1026) antibody. EGFR (Phospho-Ser1026) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TOP2A (Phospho-Ser1106) antibody. Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using TOP2A (Phospho-Ser1106) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody. Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Phospho-Tyr749/681) antibody.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Inos (Phospho-Tyr151) antibody. Inos (Phospho-Tyr151) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (PEK/PERK (Phospho-Thr981) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Application Data (Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Phospho-Ser226) antibody (#A0432).)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using GR (Phospho-Ser226) antibody (#A0432).)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Application Data (53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
IHC (Immunohiostchemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody. Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using PLCG1 (phospho-Tyr771) antibody.)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLCG1 (phospho-Tyr771) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Calnexin (Phospho-Ser583) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Phospho-Ser583) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYT1 (Phospho-Ser83) antibody. MYT1 (Phospho-Ser83) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKR (Phospho-Thr258) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using PKR (Phospho-Thr258) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody. Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho-Ser64) antibody (#A0401).)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Ser64) antibody (#A0401).)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from HeLa cells using NF-kappaB p105/p50 (Ab-893) antibody.)
IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p105/p50 (phospho-Ser893) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody. CNOT2 (Phospho-Ser101) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using 14-3-3 beta/zeta (Phospho-Ser184/186) antibody. 14-3-3 beta/zeta (Phospho-Ser184/186) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody.)
WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody.)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (phospho-Ser1619) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using ETS1 (Phospho-Thr38) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ETS1 (Phospho-Thr38) antibody. Western blot analysis of extracts from HeLa cells, treated with Serum (20%, 15mins), using ETS1 (Phospho-Thr38) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot) (Western blot analysis of extracts from Mouse brain cells, using GRIN2B (Phospho-Ser1303) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (TNF-a +- Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).)
WB (Western Blot) (Western blot analysis of extracts from Hela cells treated with TNF-alpha (20ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody.)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (phospho-Ser254) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot) (Western blot analysis of extracts from A549 cells, treated with dexamethason (10nM, 1hour), using GR (Phospho-Ser203) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC delta (Phospho-Tyr313) antibody. Western blot analysis of extracts from HuvEc cells, treated with TNF-a (20ng/ml, 30mins), using PKC delta (Phospho-Tyr313) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from 293 cells using Src (Ab-529) antibody (Line1 and 2) and Src (phospho-Tyr529) antibody (Line 3 and 4).)
IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (phospho-Tyr529) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K7 (Phospho-Ser439) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using MAP3K7 (Phospho-Ser439) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1336) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using NMDAR2B (Phospho-Tyr1336) antibody.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using PKC zeta (phospho-Thr410) antibody.)
WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKC zeta (phospho-Thr410) antibody.)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta (phospho-Thr410) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho-Ser385) antibody. Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot) (Western blot analysis of extracts from JurKat cells, using EPHA7 (Phospho-Tyr791) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody. MLK1/2 (Phospho-Thr312/266) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using TSC2 (Phospho-Tyr1571) antibody. TSC2 (Phospho-Tyr1571) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Smad1 (Phospho-Ser187) antibody. Western blot analysis of extracts from mouse muscle cells, using Smad1 (Phospho-Ser187) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody. Western blot analysis of extracts from HuvEc cells (lane 1) and HT29 cells (lane 2), using hnRPD (Phospho-Ser83) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using C-RAF (Phospho-Thr269) antibody. C-RAF (Phospho-Thr269) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody. Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Phospho-Thr222) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using Bloom Syndrome Protein (Phospho-Thr99) antibody. Western blot analysis of extracts from HepG2 cells, using Bloom Syndrome Protein (Phospho-Thr99) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody (#A0414).)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk2 (Phospho-Thr387) Antibody (#A0414).)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho-Thr3) antibody (#A0434).)
IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Histone H3 (Phospho-Thr3) antibody (#A0434).)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr701) antibody. Western blot analysis of extracts from mouse brain cells, using Trk A (Phospho-Tyr701) antibody.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5mins), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).)
IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (phospho-Thr45) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HT29 cells, using SNAI1 (Phospho-Ser246) antibody. Immunofluorescence analysis of HuvEc cells, using SNAI1 (Phospho-Ser246) antibody.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGFBP-3 (Phospho-Ser183) antibody. IGFBP-3 (Phospho-Ser183) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of LOVO cells, using HNF4alpha (Phospho-Ser313) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using HNF4alpha (Phospho-Ser313) antibody. Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HNF4alpha (Phospho-Ser313) antibody.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HeLa cells, treated with TNF (10ng/ml, 30mins), using KIF2C (Phospho-Ser95) antibody. Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Phospho-Tyr453) antibody. Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) antibody.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using GRF-1 (Phospho-Tyr1105) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using GRF-1 (Phospho-Tyr1105) antibody.)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Aquaporin 2 (Phospho-Ser256) antibody. Aquaporin 2 (Phospho-Ser256) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
What Are Phospho Antibodies?
Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.
Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.
What Are The Key Applications of Phospho Antibodies?
1. Western Blotting
One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.
2. Staining of Fixed Cells (Immunocytochemistry)
Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.
3. Specificity Tests Using Peptides
In order to make sure that the antibody is only binding to the right target:
Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).
If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.
A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.
Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.
4. Genetic Confirmation
As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.
Why Buy Phospho Antibodies Through Us?
The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:
Standard methods and proper controls in all tests to ensure high quality.
These antibodies are tested and validated in different cell types and species.
High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.
FAQ
1. What Are Phospho-Specific Antibodies?
Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.
2. How to Detect Phosphorylated Proteins in a Western Blot?
To find out if a protein is phosphorylated using Western blot:
Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.
You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).
3. How to Choose the Best Antibody?
Here are some simple tips to help you pick the right antibody:
Know your target
Match your sample characteristics
Confirm the intended use is appropriate
Check “host” and “type”
Check the “quality” of the presented data/images
Appraise whether the available validation meets your needs
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