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Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.


Viewing 4550-4600 of 5299 product results


product-image-AAA109538_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells, treated with TSA (400nM, 24hours), using EGFR (Phospho-Ser1026) antibody. EGFR (Phospho-Ser1026) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

EGFR, Antibody (Cat# AAA109538)

Full Name
EGFR (Phospho-Ser1026) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109574_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TOP2A (Phospho-Ser1106) antibody. Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using TOP2A (Phospho-Ser1106) antibody.)

TOP2A, Antibody (Cat# AAA109574)

Full Name
TOP2A (Phospho-Ser1106) Antibody
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109583_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody. Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Phospho-Tyr749/681) antibody.)

MER/SKY, Antibody (Cat# AAA109583)

Full Name
MER/SKY (Phospho-Tyr749/681) Antibody
Gene Names
MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109599_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Inos (Phospho-Tyr151) antibody. Inos (Phospho-Tyr151) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

iNOS, Antibody (Cat# AAA109599)

Full Name
iNOS (Phospho-Tyr151) Antibody
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109843_IHC13.jpg IHC (Immunohiostchemistry) (PEK/PERK (Phospho-Thr981) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

PEK/PERK, Antibody (Cat# AAA109843)

Full Name
PEK/PERK (Phospho-Thr981) Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human (Identities = 100%, Positives = 100%);
Mouse (Identities = 92%, Positives = 92%);
Rat (Identities = 92%, Positives = 92%)
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109910_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Phospho-Ser226) antibody (#A0432).)

GR, Antibody (Cat# AAA109910)

Full Name
GR (Phospho-Ser226) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109927_AD11.jpg Application Data (53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

53BP1, Antibody (Cat# AAA109927)

Full Name
53BP1 (Phospho-Ser25) Antibody
Gene Names
TP53BP1; TP53; p202; 53BP1; TDRD30; p53BP1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109931_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using PLCG1 (phospho-Tyr771) antibody.)

PLCG1, Antibody (Cat# AAA109931)

Full Name
PLCG1 (Phospho-Tyr771) Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110035_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.)

Calnexin, Antibody (Cat# AAA110035)

Full Name
Calnexin (Phospho-Ser583) Antibody
Gene Names
CANX; CNX; P90; IP90
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110044_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MYT1 (Phospho-Ser83) antibody. MYT1 (Phospho-Ser83) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

MYT1, Antibody (Cat# AAA110044)

Full Name
MYT1 (Phospho-Ser83) Antibody
Gene Names
PKMYT1; MYT1; PPP1R126
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109750_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKR (Phospho-Thr258) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using PKR (Phospho-Thr258) antibody.)

PKR, Antibody (Cat# AAA109750)

Full Name
PKR (Phospho-Thr258) Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109665_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody. Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.)

VE-Cadherin, Antibody (Cat# AAA109665)

Full Name
VE-Cadherin (Phospho-Tyr731) Antibody
Gene Names
CDH5; 7B4; CD144
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109706_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho-Ser64) antibody (#A0401).)

4E-BP1, Antibody (Cat# AAA109706)

Full Name
4E-BP1 (Phospho-Ser64) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109427_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using NF-kappaB p105/p50 (Ab-893) antibody.)

NF-kappaB p105/p50, Antibody (Cat# AAA109427)

Full Name
NF-kappaB p105/p50 (Phospho-Ser893) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110087_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody. CNOT2 (Phospho-Ser101) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

CNOT2, Antibody (Cat# AAA110087)

Full Name
CNOT2 (Phospho-Ser101) Antibody
Gene Names
CNOT2; NOT2; CDC36; NOT2H; HSPC131
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110128_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using 14-3-3 beta/zeta (Phospho-Ser184/186) antibody. 14-3-3 beta/zeta (Phospho-Ser184/186) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

14-3-3 beta/zeta, Antibody (Cat# AAA110128)

Full Name
14-3-3 beta/zeta (Phospho-Ser184/186) Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110132_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody.)

WAVE1, Antibody (Cat# AAA110132)

Full Name
WAVE1 (Phospho-Tyr125) Antibody
Gene Names
WASF1; WAVE; SCAR1; WAVE1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110323_IF11.jpg IF (Immunofluorescence) (PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody.)

POLR2A, Antibody (Cat# AAA110323)

Full Name
POLR2A (Phospho-Ser1619) Antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110331_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using ETS1 (Phospho-Thr38) antibody.)

ETS1, Antibody (Cat# AAA110331)

Full Name
ETS1 (Phospho-Thr38) Antibody
Gene Names
Ets1; vs; Tpl1; Ets-1; AI196000; AI448617; D230050P06
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110343_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from Mouse brain cells, using GRIN2B (Phospho-Ser1303) antibody.)

GRIN2B, Antibody (Cat# AAA110343)

Full Name
GRIN2B (Phospho-Ser1303) Antibody
Gene Names
GRIN2B; NR3; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110234_IF11.jpg IF (Immunofluorescence) (TNF-a +- Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).)

HSP90B, Antibody (Cat# AAA110234)

Full Name
HSP90B (Phospho-Ser254) Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110248_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from A549 cells, treated with dexamethason (10nM, 1hour), using GR (Phospho-Ser203) antibody.)

GR, Antibody (Cat# AAA110248)

Full Name
GR (Phospho-Ser203) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110253_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC delta (Phospho-Tyr313) antibody. Western blot analysis of extracts from HuvEc cells, treated with TNF-a (20ng/ml, 30mins), using PKC delta (Phospho-Tyr313) antibody.)

PKC delta, Antibody (Cat# AAA110253)

Full Name
PKC delta (Phospho-Tyr313) Antibody
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110283_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells using Src (Ab-529) antibody (Line1 and 2) and Src (phospho-Tyr529) antibody (Line 3 and 4).)

Src, Antibody (Cat# AAA110283)

Full Name
Src (Phospho-Tyr529) Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110284_IF11.gif IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using p90 RSK (Phospho-Thr573) antibody)

p90 RSK, Antibody (Cat# AAA110284)

Full Name
p90 RSK (Phospho-Thr573) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Pricing
product-image-AAA110557_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K7 (Phospho-Ser439) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using MAP3K7 (Phospho-Ser439) antibody.)

MAP3K7, Antibody (Cat# AAA110557)

Full Name
MAP3K7 (Phospho-Ser439) Antibody
Gene Names
MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110609_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1336) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using NMDAR2B (Phospho-Tyr1336) antibody.)

NMDAR2B, Antibody (Cat# AAA110609)

Full Name
NMDAR2B (Phospho-Tyr1336) Antibody
Gene Names
GRIN2B; NR3; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110638_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using PKC zeta (phospho-Thr410) antibody.)

PKC zeta, Antibody (Cat# AAA110638)

Full Name
PKC zeta (Phospho-Thr410) Antibody
Gene Names
PRKCZ; PKC2; PKC-ZETA
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110646_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.)

IRF-3, Antibody (Cat# AAA110646)

Full Name
IRF-3 (Phospho-Ser385) Antibody
Gene Names
IRF3; IIAE7
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110664_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from JurKat cells, using EPHA7 (Phospho-Tyr791) antibody.)

EPHA7, Antibody (Cat# AAA110664)

Full Name
EPHA7 (Phospho-Tyr791) Antibody
Gene Names
EPHA7; EHK3; EK11; EHK-3; HEK11
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110705_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody. MLK1/2 (Phospho-Thr312/266) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

MLK1/2, Antibody (Cat# AAA110705)

Full Name
MLK1/2 (Phospho-Thr312/266) Antibody
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110730_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using TSC2 (Phospho-Tyr1571) antibody. TSC2 (Phospho-Tyr1571) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

TSC2, Antibody (Cat# AAA110730)

Full Name
TSC2 (Phospho-Tyr1571) Antibody
Gene Names
TSC2; LAM; TSC4; PPP1R160
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110736_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Smad1 (Phospho-Ser187) antibody. Western blot analysis of extracts from mouse muscle cells, using Smad1 (Phospho-Ser187) antibody.)

Smad1, Antibody (Cat# AAA110736)

Full Name
Smad1 (Phospho-Ser187) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110765_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.)

B-RAF, Antibody (Cat# AAA110765)

Full Name
B-RAF (Phospho-Ser446) Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110513_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using hnRPD (Phospho-Ser83) antibody.)

hnRPD, Antibody (Cat# AAA110513)

Full Name
hnRPD (Phospho-Ser83) Antibody
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110516_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using C-RAF (Phospho-Thr269) antibody. C-RAF (Phospho-Thr269) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

C-RAF, Antibody (Cat# AAA110516)

Full Name
C-RAF (Phospho-Thr269) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110538_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody. Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using MAP2K3 (Phospho-Thr222) antibody.)

MAP2K3, Antibody (Cat# AAA110538)

Full Name
MAP2K3 (Phospho-Thr222) Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110195_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using Bloom Syndrome Protein (Phospho-Thr99) antibody. Western blot analysis of extracts from HepG2 cells, using Bloom Syndrome Protein (Phospho-Thr99) antibody.)

Bloom Syndrome, Antibody (Cat# AAA110195)

Full Name
Bloom Syndrome (Phospho-Thr99) Antibody
Gene Names
BLM; BS; RECQ2; RECQL2; RECQL3
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110216_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody (#A0414).)

Chk2, Antibody (Cat# AAA110216)

Full Name
Chk2 (Phospho-Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110218_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho-Thr3) antibody (#A0434).)

Histone H3, Antibody (Cat# AAA110218)

Full Name
Histone H3 (Phospho-Thr3) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110449_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr701) antibody. Western blot analysis of extracts from mouse brain cells, using Trk A (Phospho-Tyr701) antibody.)

Trk A, Antibody (Cat# AAA110449)

Full Name
Trk A (Phospho-Tyr701) Antibody
Gene Names
NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111954_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5mins), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).)

4E-BP1, Antibody (Cat# AAA111954)

Full Name
4E-BP1 (Phospho-Thr45) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111959_WB13.jpg WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HT29 cells, using SNAI1 (Phospho-Ser246) antibody. Immunofluorescence analysis of HuvEc cells, using SNAI1 (Phospho-Ser246) antibody.)

SNAI1, Antibody (Cat# AAA111959)

Full Name
SNAI1 (Phospho-Ser246) Antibody
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111974_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGFBP-3 (Phospho-Ser183) antibody. IGFBP-3 (Phospho-Ser183) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

IGFBP-3, Antibody (Cat# AAA111974)

Full Name
IGFBP-3 (Phospho-Ser183) Antibody
Gene Names
IGFBP3; IBP3; BP-53
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111996_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of LOVO cells, using HNF4alpha (Phospho-Ser313) antibody.)

HNF4alpha, Antibody (Cat# AAA111996)

Full Name
HNF4alpha (Phospho-Ser313) Antibody
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112000_WB13.jpg WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HeLa cells, treated with TNF (10ng/ml, 30mins), using KIF2C (Phospho-Ser95) antibody. Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) antibody.)

KIF2C, Antibody (Cat# AAA112000)

Full Name
KIF2C (Phospho-Ser95) Antibody
Gene Names
KIF2C; MCAK; CT139; KNSL6
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112002_WB13.jpg WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Phospho-Tyr453) antibody. Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) antibody.)

CD5, Antibody (Cat# AAA112002)

Full Name
CD5 (Phospho-Tyr453) Antibody
Gene Names
CD5; T1; LEU1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Pricing
product-image-AAA112009_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using GRF-1 (Phospho-Tyr1105) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using GRF-1 (Phospho-Tyr1105) antibody.)

GRF-1, Antibody (Cat# AAA112009)

Full Name
GRF-1 (Phospho-Tyr1105) Antibody
Gene Names
ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112011_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Aquaporin 2 (Phospho-Ser256) antibody. Aquaporin 2 (Phospho-Ser256) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Aquaporin 2, Antibody (Cat# AAA112011)

Full Name
Aquaporin 2 (Phospho-Ser256) Antibody
Gene Names
AQP2; AQP-CD; WCH-CD
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112013_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (phospho-Tyr1248) antibody.)

HER2, Antibody (Cat# AAA112013)

Full Name
HER2 (Phospho-Tyr1248) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

What Are Phospho Antibodies?


Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.


Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.


What Are The Key Applications of Phospho Antibodies?


1. Western Blotting


One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.


2. Staining of Fixed Cells (Immunocytochemistry)


Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.


3. Specificity Tests Using Peptides


In order to make sure that the antibody is only binding to the right target:


  • Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).

  • If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.

  • A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.

  • Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.


4. Genetic Confirmation


As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.


Why Buy Phospho Antibodies Through Us?


  • The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:

  • Standard methods and proper controls in all tests to ensure high quality.

  • These antibodies are tested and validated in different cell types and species.

  • High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.


FAQ


1. What Are Phospho-Specific Antibodies?


Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.


2. How to Detect Phosphorylated Proteins in a Western Blot?


To find out if a protein is phosphorylated using Western blot:


  • Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.

  • You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).


3. How to Choose the Best Antibody?


Here are some simple tips to help you pick the right antibody:


  • Know your target

  • Match your sample characteristics

  • Confirm the intended use is appropriate

  • Check “host” and “type”

  • Check the “quality” of the presented data/images

  • Appraise whether the available validation meets your needs


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