Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-CD71 (S24) Polyclonal Antibody)

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TFRC, Polyclonal Antibody (Cat# AAA235684)

Full Name

Phospho-TFRC (S24) Antibody

Gene Names

TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody)

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REL, Polyclonal Antibody (Cat# AAA235686)

Full Name

Phospho-REL (S503) Antibody

Gene Names

REL; C-Rel

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-PAK4/PAK5/PAK6 antibody at 2.15ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,75,80 KDaObserved band size: 75,80 KDa)

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PAK4/PAK5/PAK6, Monoclonal Recombinant Antibody (Cat# AAA235578)

Full Name

Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Antibody

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA018327A05phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

ICC (Immunocytochemistry) (Immunocytochemistry analysis of CSB-RA018327A05phHU diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysateAll lanes?Phospho-POLR2A antibody at 0.75ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 270 KDaObserved band size: 270 KDa)

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POLR2A, Monoclonal Recombinant Antibody (Cat# AAA235580)

Full Name

Phospho-POLR2A (S5) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA019284A621phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-RAF1 antibody at 1.525ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73 KDaObserved band size: 73 KDa)

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RAF1, Monoclonal Recombinant Antibody (Cat# AAA235585)

Full Name

Phospho-RAF1 (S621) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-RB1 in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA019386A780phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA019386A780phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of K562 cells with CSB-RA019386A780phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of CSB-RA019386A780phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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RB1, Monoclonal Recombinant Antibody (Cat# AAA235586)

Full Name

Phospho-RB1 (S780) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Immunohistochemistry

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IHC (Immunohiostchemistry) (IHC image of CSB-RA022812A705phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate,Jurkat whole cell lysate(treated with EGF or Pervanadate)All lanes?Phospho-STAT3 antibody at 0.925ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88 KDaObserved band size: 88 KDa)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA235591)

Full Name

Phospho-STAT3 (Tyr705) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of 293 cells(treated with 50mM Calyculin A for 30min) with CSB-RA024077A55phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?293 whole cell lysate(treated with Calyculin A or Pervanadate)All lanes?Phospho-TP53 antibody at 1.28ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 KDaObserved band size: 53 KDa)

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TP53, Monoclonal Recombinant Antibody (Cat# AAA235596)

Full Name

Phospho-TP53 (T55) Antibody

Gene Names

TP53; P53; BCC7; LFS1; BMFS5; TRP53

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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WB (Western Blot) (Western blot analysis of 293T 3T3 lysis using Phospho-IRS-1 (S636) antibody.)

WB (Western Blot) (Western Blot analysis of RAW using Phospho-IRS-1 (S636) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HELA 22RV1 KB NIH-3T3 cells using Phospho-IRS-1 (S636) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody)

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IRS1, Polyclonal Antibody (Cat# AAA236774)

Full Name

Phospho-IRS1 (S636) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1(Phospho-Ser383) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1(Phospho-Ser383) Antibody.)

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ELK1, Polyclonal Antibody (Cat# AAA243040)

Full Name

Phospho-ELK1 (Ser383) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PDK1(Phospho-Ser241) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1(Phospho-Ser241) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1(Phospho-Ser241) Antibody.)

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PDPK1, Polyclonal Antibody (Cat# AAA243257)

Full Name

Phospho-PDPK1 (Ser241) Antibody

Gene Names

PDPK1; PDK1; PDPK2; PRO0461

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p90RSK(Phospho-Thr348) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK(Phospho-Thr348) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK(Phospho-Thr348) Antibody.)

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RPS6KA1, Polyclonal Antibody (Cat# AAA243276)

Full Name

Phospho-RPS6KA1 (Thr348) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

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CFL1, Polyclonal Antibody (Cat# AAA243287)

Full Name

Phospho-CFL1 (Ser3) Antibody

Gene Names

CFL1; CFL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody.)

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MAPK3, Polyclonal Antibody (Cat# AAA243303)

Full Name

Phospho-MAPK3 (Tyr204) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) Antibody.)

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NF2, Polyclonal Antibody (Cat# AAA243311)

Full Name

Phospho-NF2 (Ser518) Antibody

Gene Names

NF2; ACN; SCH; BANF

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody.)

WB (Western Blot) (Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody.)

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PKM, Polyclonal Antibody (Cat# AAA243168)

Full Name

Phospho-PKM (Ser37) Antibody

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts using NF-kappaB p65 (phospho-Ser468) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p65 (phospho-Ser468) antibody.)

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RELA, Polyclonal Antibody (Cat# AAA243170)

Full Name

Phospho-RELA (Ser468) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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BLM, Polyclonal Antibody (Cat# AAA243194)

Full Name

Phospho-BLM (Thr99) Antibody

Gene Names

BLM; BS; RECQ2; RECQL2; RECQL3

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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MAP2K7, Polyclonal Antibody (Cat# AAA243210)

Full Name

Phospho-MAP2K7 (Ser271) Antibody

Gene Names

MAP2K7; MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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PLD2, Polyclonal Antibody (Cat# AAA243228)

Full Name

Phospho-PLD2 (Tyr169) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Phospho-Thr334) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2(Phospho-Thr334) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using MAPKAPK-2(Phospho-Thr334) Antibody.)

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MAPKAPK2, Polyclonal Antibody (Cat# AAA243318)

Full Name

Phospho-MAPKAPK2 (Thr334) Antibody

Gene Names

MAPKAPK2; MK2; MK-2; MAPKAP-K2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody.)

WB (Western Blot) (Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCbeta (phospho-Thr641) antibody.)

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PRKCB, Polyclonal Antibody (Cat# AAA243332)

Full Name

Phospho-PRKCB (Thr641) Antibody

Gene Names

PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Period Circadian Protein 2 (Phospho-Ser662) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 3T3lbnotHeLa and K562 cells, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Period Circadian Protein 2 (Phospho-Ser662) antibody. The lane on the right is treated with the synthesized peptide.)

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PER2, Polyclonal Antibody (Cat# AAA243347)

Full Name

Phospho-PER2 (Ser662) Antibody

Gene Names

PER2; FASPS; FASPS1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Phospho-Ser189) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Phospho-Ser189) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody.)

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MAP2K3, Polyclonal Antibody (Cat# AAA243361)

Full Name

Phospho-MAP2K3 (Ser189) Antibody

Gene Names

MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser51) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody.)

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EIF2S1, Polyclonal Antibody (Cat# AAA243370)

Full Name

Phospho-EIF2S1 (Ser51) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-GATA3 (S308) Jurkat cell lysate treated with Bromo-cAMP (AAA124531).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GATA3)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-GATA3 (S308) Antibody(AAA124531)GATA3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (AAA124531)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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GATA3, Monoclonal Antibody (Cat# AAA124531)

Full Name

Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody

Gene Names

GATA3; HDR; HDRS

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate (AAA124532).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKDC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKDC)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-DNA PKcs (S2056) Antibody(AAA124532)PRKDC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKDC Antibody (AAA124532)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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DNA PKcs, Monoclonal Antibody (Cat# AAA124532)

Full Name

Anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal Antibody

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western Blot analysis of Phospho-LAT in Jurkat cell lysate.)

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LAT, Monoclonal Antibody (Cat# AAA124536)

Full Name

Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody

Gene Names

LAT; LAT1; pp36; IMD52

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western blot analysis of Phospho-Synapsin I (S9) expression in (1) Human brain lysate; (2) Human brain lysate treated with AP (AAA124538).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SYN1)

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Synapsin I, Monoclonal Antibody (Cat# AAA124538)

Full Name

Anti-Phospho-Synapsin I (S9) Rabbit Monoclonal Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-Histone H14 (T17) expression in Jurkat cell lysate (AAA124540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H1E monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1H1E)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon, using Phospho-Histone H14 (T17) Antibody(AAA124540)HIST1H1E was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H1E Antibody (AAA124540)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Histone H1.4, Monoclonal Antibody (Cat# AAA124540)

Full Name

Anti-Phospho-Histone H1.4 (T17) Rabbit Monoclonal Antibody

Gene Names

HIST1H1E; H1E; H1.4; H1F4; RMNS; H1s-4; dJ221C16.5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-c-Myc (S62) expression in HeLa cell lysate (AAA124524).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYC)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody (AAA124524)MYC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYC Antibody (AAA124524)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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c-Myc, Monoclonal Antibody (Cat# AAA124524)

Full Name

Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody

Gene Names

MYC; MRTL; MYCC; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

Standard Curve (Sample)

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Phospho-Tyrosine Kinase 2, ELISA Kit (Cat# AAA208354)

Full Name

Mouse Phospho-Tyrosine Kinase 2 ELISA Kit

Reactivity

Mouse

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y493 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody was used at 3 ug/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated. For blotting immunoprecipitated Phospho ZAP70, anti-Phospho ZAP70 (Y493) antibody AAA211719 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies (for phosphorylation at Y319), AAA211719 (for phosphorylation at Y493), and (for phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, antibody (for total ZAP70) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

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ZAP70, Polyclonal Antibody (Cat# AAA211719)

Full Name

Rabbit anti-Phospho ZAP70 (Y493) Antibody, Affinity Purified

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human

Applications

Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

Standard Curve (Sample)

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Phospho Glycogen Synthase Kinase 3beta (PGSK3beta), ELISA Kit (Cat# AAA207259)

Full Name

Mouse Phospho Glycogen Synthase Kinase 3beta (PGSK3beta) ELISA Kit

Reactivity

Mouse

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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WB (Western Blot) (Detection of human Phospho MCM2 (S53) by western blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous HEK293T cells. For IP/WB, MCM2 was immunoprecipitated using which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody AAA211126 (lot AAA211126-4) used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)

IHC (Immunohistochemistry) (Detection of human and mouse Phospho MCM2 (S53) by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) (Cat. No. AAA211126 Lot4) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA211126)

Full Name

Rabbit anti-Phospho MCM2 (S53) Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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WB (Western Blot) (Detection of human Phospho CDK1 (Y15) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated (+) with Hydroxyurea or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonal antibody (AAA213576 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal .)

IP (Immunoprecipitation) (Detection of human Phospho CDK1 (Y15) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea (+) or mock treated (-). Antibodies: Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonal antibody (AAA213576 lot 1) used for IP at 20 ul/mg lysate. Total CDK1 was immunoprecipitated by Rabbit anti-CDK1 recombinant monoclonal antibody (BLR085G) . Immunoprecipitates where treated with phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho CDK1 (Y15), AAA213576 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal .)

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CDK1, Monoclonal Recombinant Antibody (Cat# AAA213576)

Full Name

Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H]

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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IHC (Immunohistochemisry) (Detection of human Phospho-RPA32 (S4/S8) by immunohistochemistry. Samples: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RPA32 (S4/S8) (Cat. No. AAA214014) used at a dilution of 1:100. Detection Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)

IHC (Immunohiostchemistry) (Detection of human Phospho-RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RPA32 (S4/S8) (AAA214014 Lot 4) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of mouse Phospho-RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Affinity purified rabbit anti-Phospho-RPA32 (S4/S8) (AAA214014 Lot 4) used at a dilution of 1:250. Detection: DAB)

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RPA32, Polyclonal Antibody (Cat# AAA214014)

Full Name

Rabbit anti-Phospho RPA32 (S4/S8) IHC Antibody, Affinity Purified

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.001 mg $665/0.01 mg $940/5x0.001 mg $1,295/2x0.01 mg $1,890/3x0.01 mg $2,450/4x0.01 mg $2,780/5x0.01 mg

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IHC (Immunohiostchemistry) (Detection of human Phospho-53BP1 (Ser 25) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Lambda and CIP phosphatase treated section immunostained for Phospho-53BP1. Antibodies: Affinity purified rabbit anti-Phospho-53BP1 (Ser 25) (Cat. No. AAA213756) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho-53BP1 (Ser 25) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Mock phosphatase treated section immunostained for Phospho-53BP1. Antibodies: Affinity purified rabbit anti-Phospho-53BP1 (Ser 25) (Cat. No. AAA213756) used at a dilution of 1:250. Detection: DAB)

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53BP1, Polyclonal Antibody (Cat# AAA213756)

Full Name

Rabbit anti-Phospho 53BP1 (S25) IHC Antibody, Affinity Purified

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.001 mg $665/0.01 mg $940/5x0.001 mg $1,295/2x0.01 mg $1,890/3x0.01 mg $2,450/4x0.01 mg $2,780/5x0.01 mg

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IHC (Immunohiostchemistry) (Detection of mouse Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho MCM2 (S40/S41). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA213777) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho MCM2 (S40/S41). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA213777) used at a dilution of 1:250. Detection: DAB staining using anti-rabbit IHC antibody (Cat. No. at a dilution of 1:100.)

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MCM2, Polyclonal Antibody (Cat# AAA213777)

Full Name

Rabbit anti-Phospho MCM2 (S40/S41) IHC Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $665/0.02 mg $940/5x0.002 mg $1,295/2x0.02 mg $1,890/3x0.02 mg $2,450/4x0.02 mg $2,780/5x0.02 mg

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IHC (Immunohistochemisry) (Detection of human Phospho XRCC1 (S518/T519/T523) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S518/T519/T523) (Cat. No. AAA213802) used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.)

IHC (Immunohiostchemistry) (Detection of human Phospho XRCC1 (S518/T519/T523) by immunohistochemistry. Samples: FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho XRCC1 (S518/T519/T523). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S518/T519/T523) (Cat. No. AAA213802) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of mouse Phospho XRCC1 (S518/T519/T523) by immunohistochemistry. Samples: FFPE serial sections of mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho XRCC1 (S518/T519/T523). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S518/T519/T523) (Cat. No. AAA213802) used at a dilution of 1:250. Detection: DAB)

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XRCC1, Polyclonal Antibody (Cat# AAA213802)

Full Name

Rabbit anti-Phospho XRCC1 (S518/T519/T523) IHC Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.005 mg $500/2x0.005 mg $730/3x0.005 mg $940/5x0.0005 mg $945/4x0.005 mg $2,780/5x0.005 mg

Standard Curve (Sample)

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PERK (Phospho Extracellular Signal Regulated Kinase), ELISA Kit (Cat# AAA251370)

Full Name

Human PERK (Phospho Extracellular Signal Regulated Kinase) ELISA Kit

Reactivity

Human

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

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p-tau217 (Phospho Tau 217), ELISA Kit (Cat# AAA250999)

Full Name

Mouse p-tau217 (Phospho Tau 217) ELISA Kit

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Reactivity

Mouse

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

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p-tau217 (Phospho Tau 217), ELISA Kit (Cat# AAA251072)

Full Name

Human p-tau217 (Phospho Tau 217) ELISA Kit

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

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p-tau217 (Phospho Tau 217), ELISA Kit (Cat# AAA253182)

Full Name

Mouse p-tau217 (Phospho Tau 217) QuickTest ELISA Kit

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Reactivity

Mouse

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

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p-tau181 (Phospho Tau 181), ELISA Kit (Cat# AAA253448)

Full Name

Mouse p-tau181 (Phospho Tau 181) QuickTest ELISA Kit

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Reactivity

Mouse

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser133) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133) Antibody.)

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CREB1, Polyclonal Antibody (Cat# AAA243061)

Full Name

Phospho-CREB1 (Ser133) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R(Phospho-Tyr1165/Tyr1166) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with insulin using IGF-1R(Phospho-Tyr1165/Tyr1166) Antibody.)

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IGF1R, Polyclonal Antibody (Cat# AAA243072)

Full Name

Phospho-IGF1R (Tyr1165/Tyr1166) Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser6) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 and Hela cells untreated or treated with UV using p53(Phospho-Ser6) Antibody.)

Compare (5 max)

TP53, Polyclonal Antibody (Cat# AAA243075)

Full Name

Phospho-TP53 (Ser6) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs