Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western blot analysis of extracts from A2780 cells, treated with calf intestinal phosphatase (CIP), using Chk1 (Phospho-Ser280) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1(Phospho-Ser280) Antibody.)

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CHEK1, Polyclonal Antibody (Cat# AAA243094)

Full Name

Phospho-CHEK1 (Ser280) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Phospho-Ser498) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody.)

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HDAC5, Polyclonal Antibody (Cat# AAA243110)

Full Name

Phospho-HDAC5 (Ser498) Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1172) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1172) Antibody.)

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EGFR, Polyclonal Antibody (Cat# AAA243120)

Full Name

Phospho-EGFR (Tyr1172) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody.)

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STMN1, Polyclonal Antibody (Cat# AAA243122)

Full Name

Phospho-STMN1 (Ser25) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105(Phospho-Ser932) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF-a using NFkB-p105(Phospho-Ser932) Antibody.)

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NFKB1, Polyclonal Antibody (Cat# AAA243129)

Full Name

Phospho-NFKB1 (Ser932) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells treated with UV using Chk1 (Phospho-Ser296) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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CHEK1, Polyclonal Antibody (Cat# AAA243192)

Full Name

Phospho-CHEK1 (Ser296) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody. The lane on the left is treated with antigen-specific peptide.)

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MAP3K1, Polyclonal Antibody (Cat# AAA243207)

Full Name

Phospho-MAP3K1 (Thr1402) Antibody

Gene Names

MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Detection of human Phospho-RPA32 (S4/8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-RPA32 (S4/8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin antibody .)

IP (Immunoprecipitation) (Detection of human Phospho RPA32 (S4/8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Phospho RPA32 (S4/8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533 lot 3) used for IP at 6 ul/mg lysate. Phospho RPA32 (S4/8) was also immunoprecipitated by a previous lot of this antibody (AAA213533 lot 2) and by rabbit anti-RPA32 antibody . For blotting immunoprecipitated Phospho RPA32 (S4/8), AAA213533 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Total RPA32 detected by rabbit anti-RPA32 .)

ICC (Immunocytochemistry) (Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left). Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533) used at 1:5,000. Detection: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)

ICC (Immunocytochemistry) (Detection of human Phospho-RPA32 (S4/S8) in FFPE etoposide-treated HeLa cells, mock CIP (left) and CIP (right). Antibody: Rabbit anti-phospho RPA32 (S4/S8) [BL-165-5F1] (AAA213533). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM/FACS (Flow Cytometry) (Detection of human phospho RPA32 (shaded) in etoposide treated HEK293T cells (right) and untreated HEK293T cells (right) by flow cytometry. Antibody: Rabbit anti-phospho RPA32 recombinant monoclonal [BL-165-5F1] (AAA213533) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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RPA32, Monoclonal Recombinant Antibody (Cat# AAA213533)

Full Name

Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Immunocytochemistry, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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WB (Western Blot) (Detection of human Phospho STAT3 (S727) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (IFNalpha), Jurkat, Jurkat (IFNalpha), and NCI-H226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho STAT3 (S727) recombinant monoclonal antibody (AAA213617 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human Phospho STAT3 (S727) by western blot of immunoprecipitates from HeLa cells treated with IFN-alpha (100 ng/mL). IPs were treated (+) or mock treated (-) with phosphatase. Antibodies: Rabbit anti-STAT3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blotting immunoprecipitated Phospho STAT3 (S727), AAA213617 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Total STAT3 antibody .)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA213617)

Full Name

Rabbit anti-Phospho STAT3 (S727) Recombinant Monoclonal Antibody [BLR160J]

Gene Names

STAT3; APRF; HIES

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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IHC (Immunohistochemisry) (Detection of mouse phospho-MCM2 (Ser 41) by immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho-MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:100. Detection: DAB)

IHC (Immunohiostchemistry) (Detection of human Phospho MCM2 (Ser 41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) or CIP phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human phospho-MCM2 (Ser 41) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Phospho-MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:100. Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA213731)

Full Name

Rabbit anti-Phospho MCM2 (S41) IHC Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.001 mg $665/0.01 mg $940/5x0.001 mg $1,295/2x0.01 mg $1,890/3x0.01 mg $2,450/4x0.01 mg $2,780/5x0.01 mg

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IHC (Immunohiostchemistry) (Detection of mouse Phospho SMC1 (Ser 966) by immunohistochemistry. Samples: FFPE sections of mouse squamous cell carcinoma. Mock treatment (left panel) or lambda phosphatase-treatment (right panel). Antibody: Affinity purified rabbit anti-Phospho-SMC1 (Ser 966) (Cat. No. AAA213732) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho SMC1 (Ser 966) by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment (left panel) or lambda phosphatase-treatment (right panel). Antibody: Affinity purified rabbit anti-Phospho-SMC1 (Ser 966) (Cat. No. AAA213732) used at a dilution of 1:250. Detection: DAB)

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SMC1, Polyclonal Antibody (Cat# AAA213732)

Full Name

Rabbit anti-Phospho SMC1 (S966) IHC Antibody, Affinity Purified

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.0025 mg $665/8 mL $940/5x0.0025 mg $1,295/2x8 mL $1,890/3x8 mL $2,450/4x8 mL $2,780/5x0.025 mg

Standard Curve (Sample)

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Phospho-Disabled Homolog 1, ELISA Kit (Cat# AAA209999)

Full Name

Rat Phospho-Disabled Homolog 1 ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Acetyl CoA Carboxylase, ELISA Kit (Cat# AAA209875)

Full Name

Rat Phospho-Acetyl CoA Carboxylase ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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WB (Western Blot) (Detection of human Phospho RNA Polymerase II (S5) by western blot. Samples: Whole cell lysate (2 ug) from HeLa cells prepared using NETN lysis buffer. Lysates were either mock treated (-) or treated with phosphatases (+). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (AAA212675 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-RNA Polymerase II antibody .)

IP (Immunoprecipitation) (Detection of human Phospho RNA Polymerase II (S5) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 1% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Immunoprecipitates were either mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (AAA212675 lot 3) used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S5), AAA212675 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry) (Detection of mouse Phospho RNA Polymerase II (S5) by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. AAA212675 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho RNA Polymerase II (S5) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. AAA212675 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

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RNA Polymerase II, Polyclonal Antibody (Cat# AAA212675)

Full Name

Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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WB (Western Blot) (Detection of human Chk1, Phospho (S317) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), etoposide (100uM for 4 hours), or untreated. Antibodies: Affinity purified rabbit anti-Chk1, Phospho (S317) antibody AAA212853 (lot AAA212853-1) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistochemistry) (Detection of human Chk1, Phospho (S317) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Chk1, Phospho (S317) (Cat. No. AAA212853 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)

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Chk1, Polyclonal Antibody (Cat# AAA212853)

Full Name

Rabbit anti-Phospho Chk1 (S317) Antibody, Affinity Purified

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $655/0.1 mg $940/5x0.01 mg $1,270/2x0.1 mg $1,860/3x0.1 mg $2,410/4x0.1 mg $2,715/5x0.1 mg

Standard Curve (Sample)

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Phospho-C-Jun N-Terminal Kinases (p-JNK), ELISA Kit (Cat# AAA209002)

Full Name

Rat Phospho-C-Jun N-Terminal Kinases (p-JNK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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NFKBIA, ELISA Kit (Cat# AAA209003)

Full Name

Rat Phospho-NFKBIA (Ser32/36) ELISA Kit

Gene Names

NFKBIB; IKBB; TRIP9

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-P38 Mitogen Activated Protein Kinase (p-P38MAPK), ELISA Kit (Cat# AAA209004)

Full Name

Rat Phospho-P38 Mitogen Activated Protein Kinase (p-P38MAPK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Extracellular Signal-Regulated Kinase (PERK), ELISA Kit (Cat# AAA209009)

Full Name

Rat Phospho-Extracellular Signal-Regulated Kinase (PERK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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WB (Western Blot) (Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S536) antibody AAA211745 (lot AAA211745-12) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower panel: Western blot for total RelA using rabbit anti-RelA antibody at 0.1 ug/ml with exposure time of 30 seconds.)

WB (Western Blot) (Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-), and treated with Phosphatase (+) or mock treated (-). Antibodies: Rabbit anti-RelA antibody used for IP at 6 ug/mg lysate. Affinity purified rabbit anti-Phospho RelA (S536) antibody AAA211745 (lot AAA211745-12 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel: Western blot for total RelA using affinity purified rabbit anti-RelA antibody at 0.1 ug/ml with exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human Phospho RelA (S536) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S536) (Cat. No. AAA211745 Lot12) used at a dilution of 1:200 (1ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).)

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RelA, Polyclonal Antibody (Cat# AAA211745)

Full Name

Rabbit anti-Phospho RelA (S536) Antibody, Affinity Purified

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $655/0.02 mg $940/5x0.002 mg $1,270/2x0.02 mg $1,860/3x0.02 mg $2,410/4x0.02 mg $2,715/5x0.02 mg

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WB (Western Blot) (Detection of human Phospho RelA (S529) by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RelA (S529) antibody AAA211746 (lot AAA211746-1) used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of human Phospho RelA (S529) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: RelA was immunoprecipitated with affinity purified rabbit anti-RelA antibody Immunoprecipitated RelA was treated with phosphatase or mock treated. Affinity purified rabbit anti-Phospho RelA (S529) antibody AAA211746 (lot AAA211746-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

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RelA, Polyclonal Antibody (Cat# AAA211746)

Full Name

Rabbit anti-Phospho RelA (S529) Antibody, Affinity Purified

Reactivity

Human

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $655/0.1 mg $940/5x0.01 mg $1,270/2x0.1 mg $1,860/3x0.1 mg $2,410/4x0.1 mg $2,715/5x0.1 mg

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WB (Western Blot) (Detection of human Phospho Histone H3 (pS10) by western blot. A-549, HeLa, K-562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degree C, 5% CO? for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody AAA211652 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Cat. No. Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemisry) (Detection of human and mouse Histone H3 (S10) by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10)(Cat. No. AAA211652 Lot1) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB)

ICC (Immunocytochemistry) (Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA211652 Lot1) used at a dilution of 1:2,500 (0.08ug/ml). Detection: Green fluorescent goat antirabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)

FCM/FACS (Flow Cytometry) (Detection of Phospho Histone H3 (pS10). Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 x 10^6 cells were stained with 0.4 ug anti-phospho Histone H3 (S10) [AAA211652] in a 150 ul reaction.)

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Histone H3, Polyclonal Antibody (Cat# AAA211652)

Full Name

Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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WB (Western Blot) (Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 ug) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. AAA211140) used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)

IHC (Immunohiostchemistry) (Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA211140 Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [AAA211140]in a 150 ul volume. DNA content was simultaneously analyzed via PI stain.)

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MCM2, Polyclonal Antibody (Cat# AAA211140)

Full Name

Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: ZAP70 antibody was used at 3 ug/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated. For blotting immunoprecipitated Phospho ZAP70, Phospho ZAP70 (Y319) antibody AAA211718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Phospho ZAP70 antibodies AAA211718 (phosphorylation at Y319), (phosphorylation at Y493), and (phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, ZAP70 antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 min.)

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10^6 cells stained with 1.0 ug/ml Phospho Zap70 (Y319) antibody AAA211718 in a 150 ul volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), anti-phospho Zap70 (Y319) in H2O2 treated (blue).)

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ZAP70, Polyclonal Antibody (Cat# AAA211718)

Full Name

Rabbit anti-Phospho ZAP70 (Y319) Antibody, Affinity Purified

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (Detection of mouse Phospho MCM2 (Ser108) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody (AAA210759 lot 5) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-MCM2 antibody .)

WB (Western Blot) (Detection of human Phospho MCM2 (Ser108) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody (AAA210759 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-MCM2 antibody .)

IP (Immunoprecipitation) (Detection of human Phospho MCM2 (Ser108) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells treated with etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-MCM2 antibody used for IP at 6 ug per reaction. Following immunoprecipitation, the IP reaction was treated with phosphatases (+) or mock treated (-). For blotting immunoprecipitated Phospho MCM2 (Ser108), AAA210759 lot 5 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-MCM2 antibody .)

IHC (Immunohiostchemistry) (Detection of mouse Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) (Cat. No. AAA210759 Lot5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) (Cat. No. AAA210759 Lot5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA210759)

Full Name

Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IHC (Immunohiostchemistry) (Detection of human Phospho Histone H3 (S10) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA213763) used at a dilution of 1:250. Detection: DAB)

ICC (Immunocytochemistry) (Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated cells (left) or calf intestinal phosphatase-treated cells (right) immunostained for Phospho Histone H3 (S10). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA213763) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)

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Histone H3, Polyclonal Antibody (Cat# AAA213763)

Full Name

Rabbit anti-Phospho Histone H3 (S10) IHC Antibody, Affinity Purified

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $500/2x0.002 mg $730/3x0.002 mg $945/4x0.002 mg $2,780/5x0.002 mg

Standard Curve (Sample)

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phospho Forkhead Box Protein O3a (p-FOXO3a), ELISA Kit (Cat# AAA181816)

Full Name

Rat phospho Forkhead Box Protein O3a (p-FOXO3a) ELISA Kit

Reactivity

Rat

Pricing

$370/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,250/5x96-Strip-Wells $4,130/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Tyrosine Kinase, ELISA Kit (Cat# AAA206061)

Full Name

Human Phospho-Tyrosine Kinase ELISA Kit

Reactivity

Human

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human appendix tissue with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:2000)

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Stat3, Polyclonal Antibody (Cat# AAA171642)

Full Name

Phospho-Stat3 (Y705) Polyclonal Antibody

Gene Names

STAT3; APRF; HIES; ADMIO

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of HT-29+PMA cells with Phospho-Cdk5 (Tyr15) Polyclonal Antibody)

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Cdk5, Polyclonal Antibody (Cat# AAA171669)

Full Name

Phospho-Cdk5 (Y15) Polyclonal Antibody

Gene Names

CDK5; LIS7; PSSALRE

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human kidney tissue with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:2000)

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Catenin-beta, Polyclonal Antibody (Cat# AAA171712)

Full Name

Phospho-Catenin-beta (S37) Polyclonal Antibody

Gene Names

ctnnb1-b; ctnnb; ctnnb1; beta-catenin

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat lung tissue with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:2000)

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Tau, Polyclonal Antibody (Cat# AAA171627)

Full Name

Phospho-Tau (S396) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat testis tissue with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:500)

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Histone H2A.X, Polyclonal Antibody (Cat# AAA171631)

Full Name

Phospho-Histone H2A.X (S139) Polyclonal Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of HepG2 cells with Phospho-Ah Receptor (Ser36) Polyclonal Antibody)

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Ah Receptor, Polyclonal Antibody (Cat# AAA171901)

Full Name

Phospho-Ah Receptor (S36) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of Jurkat cells with Phospho-EphA7 (Tyr791) Polyclonal Antibody)

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EphA7, Polyclonal Antibody (Cat# AAA171913)

Full Name

Phospho-EphA7 (Y791) Polyclonal Antibody

Gene Names

EPHA7; EHK3; EK11; EHK-3; HEK11

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse liver tissue with Phospho-p38 (Thr180/Tyr182) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon tissue with Phospho-p38 (Thr180/Tyr182) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-p38 (Thr180/Tyr182) Polyclonal Antibody at dilution of 1:1000)

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p38, Polyclonal Antibody (Cat# AAA171964)

Full Name

Phospho-p38 (T180/Y182) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of Jurkat cells with Phospho-CRMP-2 (Thr509) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-CRMP-2 (Thr509) Polyclonal Antibody at dilution of 1:1000)

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CRMP-2, Polyclonal Antibody (Cat# AAA171972)

Full Name

Phospho-CRMP-2 (T509) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human lung tissue with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat liver tissue with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:500)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA171974)

Full Name

Phospho-NFkappaB-p65 (S529) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-CaMKII?/?/? (Thr287) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue with Phospho-CaMKII?/?/? (Thr287) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 22RV1 cells with Phospho-CaMKII?/?/? (Thr287) Polyclonal Antibody at dilution of 1:500)

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CaMKIIbeta/gamma/delta, Polyclonal Antibody (Cat# AAA172019)

Full Name

Phospho-CaMKIIbeta/gamma/delta (T287) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-p53 (Thr18) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue with Phospho-p53 (Thr18) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-p53 (Thr18) Polyclonal Antibody at dilution of 1:2000)

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p53, Polyclonal Antibody (Cat# AAA172225)

Full Name

Phospho-p53 (T18) Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat spleen tissue with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon tissue with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of HT-29 cells with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:1000)

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p53, Polyclonal Antibody (Cat# AAA172248)

Full Name

Phospho-p53 (S15) Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of 3T3 cells with Phospho-PKC delta (Thr507) Polyclonal Antibody)

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PKC delta, Polyclonal Antibody (Cat# AAA172273)

Full Name

Phospho-PKC delta (T507) Polyclonal Antibody

Gene Names

PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

WB (Western Blot) (Western Blot analysis of various cells with Phospho-FoxO1 (Ser319) Polyclonal Antibody)

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FoxO1, Polyclonal Antibody (Cat# AAA172278)

Full Name

Phospho-FoxO1 (S319) Polyclonal Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat heart tissue with Phospho-eIF2? (Ser51) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-eIF2? (Ser51) Polyclonal Antibody at dilution of 1:2000)

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eIF2alpha, Polyclonal Antibody (Cat# AAA171881)

Full Name

Phospho-eIF2alpha (S51) Polyclonal Antibody

Gene Names

Eif2s1; 35kDa; Eif2a; eIF2alpha; 0910001O23Rik; 2410026C18Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon tissue with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of COLO205 cells with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:2000)

Compare (5 max)

NFkappaB-p65, Polyclonal Antibody (Cat# AAA171756)

Full Name

Phospho-NFkappaB-p65 (S536) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse liver tissue with Phospho-JAK1 (Tyr1022) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue with Phospho-JAK1 (Tyr1022) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of A549 cells with Phospho-JAK1 (Tyr1022) Polyclonal Antibody at dilution of 1:500)

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JAK1, Polyclonal Antibody (Cat# AAA172298)

Full Name

Phospho-JAK1 (Y1022) Polyclonal Antibody

Gene Names

jak1; wu:fb93e10

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human stomach tissue with Phospho-p38 (Tyr323) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain with Phospho-p38 (Tyr323) Polyclonal Antibody at dilution of 1:50)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-p38 (Tyr323) Polyclonal Antibody at dilution of 1:500)

Compare (5 max)

p38, Polyclonal Antibody (Cat# AAA172301)

Full Name

Phospho-p38 (Y323) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Rat kidney tissue with Phospho-TGF? RI (Ser165) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver tissue with Phospho-TGF? RI (Ser165) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of Mouse liver with Phospho-TGF? RI (Ser165) Polyclonal Antibody at dilution of 1:1000)

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TGFbeta RI, Polyclonal Antibody (Cat# AAA172307)

Full Name

Phospho-TGFbeta RI (S165) Polyclonal Antibody

Gene Names

TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colon tissue with Phospho-NF?B-p65 (Ser276) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus cancer tissue with Phospho-NF?B-p65 (Ser276) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-NF?B-p65 (Ser276) Polyclonal Antibody at dilution of 1:1000)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA172335)

Full Name

Phospho-NFkappaB-p65 (S276) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs