Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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WB (Western Blot) (Western Blot analysis of Jurkat cells with Phospho-CRMP-2 (Thr509) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-CRMP-2 (Thr509) Polyclonal Antibody at dilution of 1:1000)

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CRMP-2, Polyclonal Antibody (Cat# AAA171972)

Full Name

Phospho-CRMP-2 (T509) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human lung tissue with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat liver tissue with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-NF?B-p65 (Ser529) Polyclonal Antibody at dilution of 1:500)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA171974)

Full Name

Phospho-NFkappaB-p65 (S529) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human kidney tissue with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-Catenin-? (Ser37) Polyclonal Antibody at dilution of 1:2000)

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Catenin-beta, Polyclonal Antibody (Cat# AAA171712)

Full Name

Phospho-Catenin-beta (S37) Polyclonal Antibody

Gene Names

ctnnb1-b; ctnnb; ctnnb1; beta-catenin

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat heart tissue with Phospho-eIF2? (Ser51) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-eIF2? (Ser51) Polyclonal Antibody at dilution of 1:2000)

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eIF2alpha, Polyclonal Antibody (Cat# AAA171881)

Full Name

Phospho-eIF2alpha (S51) Polyclonal Antibody

Gene Names

Eif2s1; 35kDa; Eif2a; eIF2alpha; 0910001O23Rik; 2410026C18Rik

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of HepG2 cells with Phospho-Ah Receptor (Ser36) Polyclonal Antibody)

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Ah Receptor, Polyclonal Antibody (Cat# AAA171901)

Full Name

Phospho-Ah Receptor (S36) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of Jurkat cells with Phospho-EphA7 (Tyr791) Polyclonal Antibody)

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EphA7, Polyclonal Antibody (Cat# AAA171913)

Full Name

Phospho-EphA7 (Y791) Polyclonal Antibody

Gene Names

EPHA7; EHK3; EK11; EHK-3; HEK11

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat lung tissue with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Tau (Ser396) Polyclonal Antibody at dilution of 1:2000)

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Tau, Polyclonal Antibody (Cat# AAA171627)

Full Name

Phospho-Tau (S396) Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat testis tissue with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of 293T cells with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:500)

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Histone H2A.X, Polyclonal Antibody (Cat# AAA171631)

Full Name

Phospho-Histone H2A.X (S139) Polyclonal Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human appendix tissue with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of various cells with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:2000)

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Stat3, Polyclonal Antibody (Cat# AAA171642)

Full Name

Phospho-Stat3 (Y705) Polyclonal Antibody

Gene Names

STAT3; APRF; HIES; ADMIO

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

WB (Western Blot) (Western Blot analysis of HT-29+PMA cells with Phospho-Cdk5 (Tyr15) Polyclonal Antibody)

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Cdk5, Polyclonal Antibody (Cat# AAA171669)

Full Name

Phospho-Cdk5 (Y15) Polyclonal Antibody

Gene Names

CDK5; LIS7; PSSALRE

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:200)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human colon tissue with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of COLO205 cells with Phospho-NF?B-p65 (Ser536) Polyclonal Antibody at dilution of 1:2000)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA171756)

Full Name

Phospho-NFkappaB-p65 (S536) Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

Standard Curve (Sample)

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phospho Forkhead Box Protein O3a (p-FOXO3a), ELISA Kit (Cat# AAA181816)

Full Name

Rat phospho Forkhead Box Protein O3a (p-FOXO3a) ELISA Kit

Reactivity

Rat

Pricing

$370/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,250/5x96-Strip-Wells $4,130/10x96-Strip-Wells

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WB (Western Blot) (Detection of human Phospho RNA Polymerase II (S5) by western blot. Samples: Whole cell lysate (2 ug) from HeLa cells prepared using NETN lysis buffer. Lysates were either mock treated (-) or treated with phosphatases (+). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (AAA212675 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-RNA Polymerase II antibody .)

IP (Immunoprecipitation) (Detection of human Phospho RNA Polymerase II (S5) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 1% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Immunoprecipitates were either mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (AAA212675 lot 3) used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S5), AAA212675 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry) (Detection of mouse Phospho RNA Polymerase II (S5) by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. AAA212675 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho RNA Polymerase II (S5) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) (Cat. No. AAA212675 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

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RNA Polymerase II, Polyclonal Antibody (Cat# AAA212675)

Full Name

Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

WB (Western Blot) (Western blot analysis of ATM (pS1981) expression in Hela (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)

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ATM (pS1981), Polyclonal Antibody (Cat# AAA104820)

Full Name

Anti-ATM (pS1981) Phospho Antibody

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of CREB (pS121) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of CREB (pS121) expression in HepG2 colchicine-treated (A), mouse kidney (B), rat kidney (C) whole cell lysates.)

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CREB (pS121), Polyclonal Antibody (Cat# AAA104831)

Full Name

Anti-CREB (pS121) Phospho Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of c-CBL (pY774) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of c-CBL (pY774) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of c-CBL (pY774) expression in Jurkat (A), mouse liver (B), rat liver (C) whole cell lysates.)

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c-CBL (pY774), Polyclonal Antibody (Cat# AAA104867)

Full Name

Anti-c-CBL (pY774) Phospho Antibody

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of FOXO1 (pS319) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of FOXO1 (pS319) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of FOXO1 (pS319) expression in MCF7 (A), HeLa (B), NIH3T3 (C) whole cell lysates.)

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FOXO1 (pS319), Polyclonal Antibody (Cat# AAA104744)

Full Name

Anti-FOXO1 (pS319) Phospho Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat, Bovine, Monkey, Pig

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of EIF2S1 (pS51) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of EIF2S1 (pS51) expression in HeLa (A), PC12 (B), A431 (C) whole cell lysates.)

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EIF2S1 (pS51), Polyclonal Antibody (Cat# AAA105048)

Full Name

Anti-EIF2S1 (pS51) Phospho Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of CDK2 (pT160) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of CDK2 (pT160) expression in NIH3T3 (A), HeLa (B), COLO205 (C), K562 (D), A2780 (E) whole cell lysates.)

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CDK2 (pT160), Polyclonal Antibody (Cat# AAA104926)

Full Name

Anti-CDK2 (pT160) Phospho Antibody

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Sheep, Zebrafish

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

WB (Western Blot) (Western blot analysis of eNOS (pS1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates.)

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eNOS (pS1177), Polyclonal Antibody (Cat# AAA104941)

Full Name

Anti-eNOS (pS1177) Phospho Antibody

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NCoA2 (Phospho-Ser736) antibody. Western blot analysis of extracts from HeLa cells, treated with TSA (400nM, 24hours), using NCoA2 (Phospho-Ser736) antibody.)

Application Data (P-peptide-+)

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NCoA2, Antibody (Cat# AAA109933)

Full Name

NCoA2 (Phospho-Ser736) Antibody

Gene Names

NCOA2; SRC2; TIF2; GRIP1; KAT13C; NCoA-2; bHLHe75

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from MCF7 cells, using PKCdelta (Ab-645) antibody (Line 1 and 2) and PKCdelta (phospho-Ser645) antibody (Line 3 and 4).)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCdelta (phospho-Ser645) antibody.)

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PKC delta, Antibody (Cat# AAA110004)

Full Name

PKC delta (Phospho-Ser645) Antibody

Gene Names

PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) antibody. Western blot analysis of extracts from Jurkat cells, using MAP3K1 (Phospho-Thr1402) antibody.)

Application Data (P-peptide-+)

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MAP3K1, Antibody (Cat# AAA110095)

Full Name

MAP3K1 (Phospho-Thr1402) Antibody

Gene Names

MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (HRP) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-beta/gamma/delta (Phospho-Thr287) antibody. Western blot analysis of extracts from rat brain cells, using CaMK2-beta/gamma/delta (Phospho-Thr287) antibody.)

Application Data (P-peptide-+)

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CaMK2-beta/gamma/delta, Antibody (Cat# AAA110119)

Full Name

CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody

Gene Names

CAMK2B; CAM2; CAMK2; CAMKB; MRD54

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with UV, using c-Jun (phospho-Ser243) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho-Ser243) antibody.)

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c-Jun, Antibody (Cat# AAA110151)

Full Name

c-Jun (Phospho-Ser243) Antibody

Gene Names

JUN; AP1; p39; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against nonphosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Phospho-Thr383) antibody. Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) antibody.)

Application Data

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Chk2, Antibody (Cat# AAA110155)

Full Name

Chk2 (Phospho-Thr383) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot) (Western blot analysis of extracts from JurKat cells, using EPHA4 (Phospho-Tyr596) antibody.)

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EPHA4, Antibody (Cat# AAA110163)

Full Name

EPHA4 (Phospho-Tyr596) Antibody

Gene Names

EPHA4; SEK; HEK8; TYRO1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody. Nuclear Receptor NR4A1 (Phospho-Ser351) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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Nuclear Receptor NR4A1, Antibody (Cat# AAA109704)

Full Name

Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.)

Application Data (P-peptide-+)

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4E-BP1, Antibody (Cat# AAA109712)

Full Name

4E-BP1 (Phospho-Thr69) Antibody

Gene Names

ESPL1; ESP1; SEPA

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot) (Western blot analysis of extracts from JurKat cells, using HCK (Phospho-Tyr521) antibody.)

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HCK, Antibody (Cat# AAA109719)

Full Name

HCK (Phospho-Tyr521) Antibody

Gene Names

HCK; JTK9; p59Hck; p61Hck

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using CtBP1 (Phospho-Ser422) antibody.)

Application Data (P-peptide-+)

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CtBP1, Antibody (Cat# AAA109722)

Full Name

CtBP1 (Phospho-Ser422) Antibody

Gene Names

CTBP1; BARS; HADDTS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody. CAD (Phospho-Thr456) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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CAD, Antibody (Cat# AAA109741)

Full Name

CAD (Phospho-Thr456) Antibody

Gene Names

CAD; CDG1Z; GATD4; EIEE50

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ATR (Phospho-Ser428) antibody. ATR (Phospho-Ser428) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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ATR, Antibody (Cat# AAA109512)

Full Name

ATR (Phospho-Ser428) Antibody

Gene Names

ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1

Reactivity

Human

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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WB (Western Blot) (Western blot analysis of extracts from 293 cells using G3BP-1 (Ab-232) antibody (Line 1 and 2) and G3BP-1 (phospho-Ser232) antibody (Line 3 and 4).)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (phospho-Ser232) antibody.)

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G3BP-1, Antibody (Cat# AAA110016)

Full Name

G3BP-1 (Phospho-Ser232) Antibody

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody. EPHA3/4/5 (Phospho-Tyr779/833) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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EPHA3/4/5, Antibody (Cat# AAA110046)

Full Name

EPHA3/4/5 (Phospho-Tyr779/833) Antibody

Gene Names

EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody.)

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MAP3K8, Antibody (Cat# AAA109774)

Full Name

MAP3K8 (Phospho-Ser400) Antibody

Gene Names

MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody. Western blot analysis of extracts from LOVO cells, treated with starved (24hours), using PAK1/2 (Phospho-Ser199) antibody.)

Application Data (P-peptide-+)

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PAK1, Antibody (Cat# AAA109782)

Full Name

PAK1 (Phospho-Ser199) Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells using STAT3 (phospho-Tyr705) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (phospho-Tyr705) antibody.)

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STAT3, Antibody (Cat# AAA109871)

Full Name

STAT3 (Phospho-Tyr705) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) antibody. Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using Gab2 (Phospho-Ser623) antibody.)

Application Data (P-peptide-+)

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Gab2, Antibody (Cat# AAA109887)

Full Name

Gab2 (Phospho-Ser623) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (HRP) $580/0.1 mL (AF594) $580/0.1 mL (AF647) $580/0.1 mL (AF680) $580/0.1 mL (AF546) $580/0.1 mL (AF405L) $580/0.1 mL (AF405S) $580/0.1 mL (AF610) $580/0.1 mL (AF635) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody. Western blot analysis of extracts from 3T3 cells, using PYK2 (Phospho-Tyr579) antibody.)

Application Data (P-peptide-+)

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PYK2, Antibody (Cat# AAA109671)

Full Name

PYK2 (Phospho-Tyr579) Antibody

Gene Names

PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using SRF (Phospho-Ser77) antibody.)

Application Data (P-peptide-+)

Compare (5 max)

SRF, Antibody (Cat# AAA109504)

Full Name

SRF (Phospho-Ser77) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from COS7 cells using NF-kappaB p65 (Ab-435) antibody and NF-kappaB p65 (phospho-Thr435) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (phospho-Thr435) antibody.)

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NFkappaB-p65, Antibody (Cat# AAA111146)

Full Name

NFkappaB-p65 (Phospho-Thr435) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from MCF7, 293, A431, A2780 and Hela cells, using p44/42 MAP Kinase (Ab-202) antibody and p44/42 MAP Kinase (phospho-Thr202) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (phospho-Thr202) antibody.)

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p44/42 MAP Kinase, Antibody (Cat# AAA111147)

Full Name

p44/42 MAP Kinase (Phospho-Thr202) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot) (Western blot analysis of extract from HUVEC cell treated with TNF-a, using HSF1 (Phospho-Ser307) Antibody.)

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HSF1, Antibody (Cat# AAA111154)

Full Name

HSF1 (Phospho-Ser307) Antibody

Gene Names

HSF1; HSTF1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extract from 293 cells treated or untreated with UV, using SEK1/MKK4 (phospho-Ser80) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4 (phospho-Ser80) antibody.)

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SEK1/MKK4, Antibody (Cat# AAA111159)

Full Name

SEK1/MKK4 (Phospho-Ser80) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4(phospho-Thr261)antibody.)

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SEK1/MKK4, Antibody (Cat# AAA111171)

Full Name

SEK1/MKK4 (Phospho-Thr261) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau (Ab-262) antibody and Tau (phospho-Ser262) antibody.)

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Tau, Antibody (Cat# AAA111172)

Full Name

Tau (Phospho-Ser262) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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WB (Western Blot) (Western blot analysis of extracts from COS7 cells treated with UV. Left: Using P53 (phospho-Ser20) antibody. Right: Using the same antibody preincubated with synthesized peptide.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho-Ser20) antibody.)

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P53, Antibody (Cat# AAA111181)

Full Name

P53 (Phospho-Ser20) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis using BCL-XL (phospho-Ser62) antibody.)

IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-XL (phospho-Ser62) antibody.)

Compare (5 max)

BCL-XL, Antibody (Cat# AAA111191)

Full Name

BCL-XL (Phospho-Ser62) Antibody

Gene Names

BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs