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Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.


Viewing 5150-5200 of 5299 product results


product-image-AAA111197_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Tsc2 (phospho-Thr1462) antibody.P-Peptide: - / +)

Tsc2, Antibody (Cat# AAA111197)

Full Name
Tsc2 (Phospho-Thr1462) Antibody
Gene Names
TSC2; LAM; TSC4; PPP1R160
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111209_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells using BAD (phospho-Ser112) antibody.)

BAD, Antibody (Cat# AAA111209)

Full Name
BAD (Phospho-Ser112) Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111211_WB13.jpg WB (Western Blot) (Anisomycin + +p-peptide - +Western blot analysis of extract from C6 cells (Rat)using JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody.)

JNK1/JNK2, Antibody (Cat# AAA111211)

Full Name
JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111225_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells using Histone H3.1 (Ab-10) antibody and Histone H3.1 (phospho-Ser10) antibody.)

Histone H3.1, Antibody (Cat# AAA111225)

Full Name
Histone H3.1 (Phospho-Ser10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111321_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) antibody.)

p47 phox, Antibody (Cat# AAA111321)

Full Name
p47 phox (Phospho-Ser359) Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111339_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from JK cells, using VAV3 (Phospho-Tyr173) antibody.)

VAV3, Antibody (Cat# AAA111339)

Full Name
VAV3 (Phospho-Tyr173) Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111234_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with UV using c-Jun (phospho-Thr93) antibody.)

c-Jun, Antibody (Cat# AAA111234)

Full Name
c-Jun (Phospho-Thr93) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

Shc1, Antibody (Cat# AAA111256)

Full Name
Shc1 (Phospho-Tyr427) Antibody
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111262_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser807) antibody.)

Rb, Antibody (Cat# AAA111262)

Full Name
Rb (Phospho-Ser807) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111266_IHC15.jpg IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (phospho-Ser129) antibody.)

CREB, Antibody (Cat# AAA111266)

Full Name
CREB (Phospho-Ser129) Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110902_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells treated with PDGF. Left: Using PDGFR beta (phospho-Tyr751) antibody. Right: Using the same antibody preincubated with synthesized peptide.)

PDGF Receptor beta, Antibody (Cat# AAA110902)

Full Name
PDGF Receptor beta (Phospho-Tyr751) Antibody
Gene Names
PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110914_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells, using p62Dok (Ab-398) antibody and p62Dok (phospho-Tyr398) antibody.)

p62Dok, Antibody (Cat# AAA110914)

Full Name
p62Dok (phospho-Tyr398) Antibody
Gene Names
DOK1; pp62; P62DOK
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110922_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from ovary cancer cells using MEK2 (phospho-Thr394) antibody.)

MEK-2, Antibody (Cat# AAA110922)

Full Name
MEK-2 (Phospho-Thr394) Antibody
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110928_WB13.jpg WB (Western Blot) (Western blot analysis of extract from NIH/3T3 cells using HDAC5 (Ab-498) antibody and HDAC5 (phospho-Ser498) antibody.)

HDAC5, Antibody (Cat# AAA110928)

Full Name
HDAC5 (Phospho-Ser498) Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
Pricing
product-image-AAA110935_WB13.jpg WB (Western Blot) (Western blot analysis using Akt (phospho-Thr308) antibody : Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.)

Akt, Antibody (Cat# AAA110935)

Full Name
Akt (Phospho-Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110939_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using HSP27 (Ab-78) antibody and HSP27 (phospho-Ser78) antibody.)

HSP27, Antibody (Cat# AAA110939)

Full Name
HSP27 (Phospho-Ser78) Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110941_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells, untreated or treated with PMA (1ng/ml, 10min), using PKCbeta (Ab-641) antibody and PKCbeta (phospho-Thr641) antibody.)

PKCbeta, Antibody (Cat# AAA110941)

Full Name
PKCbeta (Phospho-Thr641) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110946_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with insulin(100nM, 30min) or PMA (0.2muM, 15min) using IRS-1 (Ab-636) antibody and IRS-1 (phospho-Ser636) antibody.)

IRS-1, Antibody (Cat# AAA110946)

Full Name
IRS-1(Phospho-Ser636) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110949_WB12.jpg WB (Western Blot) (Western blot analysis using IGF-1R (Ab-1161) antibody and IGF-1R (phospho-Tyr1161) antibody.)

IGF-1R, Antibody (Cat# AAA110949)

Full Name
IGF-1R (Phospho-Tyr1161) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110952_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells using HSP27 (Ab-15) antibody and HSP27 (phospho-Ser15) antibody.)

HSP27, Antibody (Cat# AAA110952)

Full Name
HSP27 (Phospho-Ser15) Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110967_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from SK-OV3 cells using HER2 (Ab-1221/1222) antibody and HER2 (phospho-Tyr1221/Tyr1222) antibody.)

HER2, Antibody (Cat# AAA110967)

Full Name
HER2 (Phospho-Tyr1221/Tyr1222) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111070_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using STAT4 (phospho-Tyr693) antibody.)

STAT4, Antibody (Cat# AAA111070)

Full Name
STAT4 (Phospho-Tyr693) Antibody
Gene Names
STAT4; SLEB11
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

ASK1, Antibody (Cat# AAA111080)

Full Name
ASK1 (Phospho-Ser966) Antibody
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

eIF4G, Antibody (Cat# AAA111082)

Full Name
eIF4G (phospho-Ser1232) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111087_IHC15.jpg IHC (Immunohistochemistry) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BRCA1 (phospho-Ser1524) antibody.)

BRCA1, Antibody (Cat# AAA111087)

Full Name
BRCA1 (Phospho-Ser1524) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111097_WB15.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells, untreated or treated with vanadate (1mg/ml, 30min), using MyoD (phospho-Ser200) antibody.)

MyoD, Antibody (Cat# AAA111097)

Full Name
MyoD (Phospho-Ser200) Antibody
Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111098_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using MEK1 (Ab-221) antibody and MEK1 (phospho-Ser221) antibody.)

MEK1, Antibody (Cat# AAA111098)

Full Name
MEK1 (Phospho-Ser221) Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111104_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293 cells, untreated Western blot analysis of extracts from 293 cells untreated or treated with serum for the indicated times, using p70 S6 Kinase (phospho-Ser424) antibody.)

p70 S6 Kinase, Antibody (Cat# AAA111104)

Full Name
p70 S6 Kinase (Phospho-Ser424) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

eIF4B, Antibody (Cat# AAA111105)

Full Name
eIF4B (phospho-Ser422) Antibody
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111122_WB13.gif WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with UV using c-Jun (phospho-Ser243) antibody.)

c-Jun, Antibody (Cat# AAA111122)

Full Name
c-Jun (Phospho-Ser243) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against nonphosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111125_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from SW 626 cells, untreated or treated with Calyculin A (50nM, 30min), using beta-Catenin (Ab-33) antibody and beta-Catenin (phospho-Ser33) antibody.)

beta-Catenin, Antibody (Cat# AAA111125)

Full Name
beta-Catenin (Phospho-Ser33) Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111133_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with UV using Elk-1 (phospho-Ser383) antibody.)

Elk-1, Antibody (Cat# AAA111133)

Full Name
Elk-1 (Phospho-Ser383) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110985_WB13.gif WB (Western Blot) (Western blot analysis of extract from HeLa cells, using STAT6 (phospho-Tyr641) antibody)

STAT6, Antibody (Cat# AAA110985)

Full Name
STAT6 (Phospho-Tyr641) Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110989_WB13.jpg WB (Western Blot) (Western blot analysis of extract from 293 cells, untreated or treated with TNF-alpha (20ng/ml, 15min), using IkB-epsilon (Ab-22) antibody and IkB-epsilon (Phospho-Ser22) antibody.)

IkB-epsilon, Antibody (Cat# AAA110989)

Full Name
IkB-epsilon (Phospho-Ser22) Antibody
Gene Names
NFKBIE; IKBE
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110993_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, untreated or treated with GM-CSF (25ng/ml 30min) using p70 S6 Kinase (phospho-Thr421) antibody.)

p70 S6 Kinase, Antibody (Cat# AAA110993)

Full Name
p70 S6 Kinase (Phospho-Thr421) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110996_WB13.gif WB (Western Blot) (Western blot analysis of extracts from SK-OV3 cells using VEGFR2 (Ab-951) antibody (Line 1 and 2) and VEGFR2 (phospho-Tyr951) antibody (Line 3 and 4).)

VEGFR2, Antibody (Cat# AAA110996)

Full Name
VEGFR2 (Phospho-Tyr951) Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111006_WB13.jpg WB (Western Blot) (Western blot analysis of extract from Hela cells untreated or treated with IFN, using NFkappaB-p65 (Ab-311) antibody and NFkappaB-p65 (phospho-Ser311) antibody.)

NFkappaB-p65, Antibody (Cat# AAA111006)

Full Name
NFkappaB-p65 (phospho-Ser311) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

Synaptotagmin II, Antibody (Cat# AAA111012)

Full Name
Synaptotagmin II (Phospho-Thr202) Antibody
Gene Names
SYT2; CMS7; MYSPC; SytII
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111018_WB13.gif WB (Western Blot) (Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea, using p53 (Ab-15) antibody (Line1 and 2) and p53 (phospho-Ser15) antibody (Line3 and 4).)

p53, Antibody (Cat# AAA111018)

Full Name
p53 (Phospho-Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111028_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from NIH-3T3 (Line 1, 4, 7 and 10) and cos7 (Line 2, 5, 8 and 11 and K562 (Line 3, 6, 9 and 12) cells, untreated or treated with UV (20min), using P38 MAPK (Ab-182) antibody and P38 MAPK (phospho-Tyr182) antibody.)

P38 MAPK, Antibody (Cat# AAA111028)

Full Name
P38 MAPK (Phospho-Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111047_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using FLT3 (Phospho-Tyr591) Antibody.)

FLT3, Antibody (Cat# AAA111047)

Full Name
FLT3 (Phospho-Tyr591) Antibody
Gene Names
FLT3; FLK2; STK1; CD135; FLK-2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111454_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using RBL1 (Phospho-Thr369) antibody. RBL1 (Phospho-Thr369) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

RBL1, Antibody (Cat# AAA111454)

Full Name
RBL1 (Phospho-Thr369) Antibody
Gene Names
RBL1; PRB1; p107; CP107
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111456_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, using MEK1 (Ab-221) antibody (Line 1 and 2) and MEK1 (phospho-Ser221) antibody (Line 3 and 4).)

MEK1/2, Antibody (Cat# AAA111456)

Full Name
MEK1/2 (Phospho-Ser221) Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111458_AD11.jpg Application Data (HMG14 (Phospho-Ser21) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

HMG14, Antibody (Cat# AAA111458)

Full Name
HMG14 (Phospho-Ser21) Antibody
Gene Names
HMGN1; HMG14
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110840_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody. Western blot analysis of extracts from 3T3 cells, treated with UV (15mins), using PAK1 (Phospho-Ser204) antibody.)

PAK1, Antibody (Cat# AAA110840)

Full Name
PAK1 (Phospho-Ser204) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110890_WB13.jpg WB (Western Blot) (Western blot analysis of extract from HeLa cells untreated or treated with heat shock (30min), using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody.)

AMPK1/AMPK2, Antibody (Cat# AAA110890)

Full Name
AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA110817_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from K562 cells treated with Insulin (0.01U/ml, 15mins), using ACC1 (Phospho-Ser80) antibody (#A0405).)

ACC1, Antibody (Cat# AAA110817)

Full Name
ACC1 (Phospho-Ser80) Antibody
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109360_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from COLO cells, treated with insulin (0.01U/ml, 15mins), using Bcl-6 (Phospho-Ser333) antibody.)

Bcl-6, Antibody (Cat# AAA109360)

Full Name
Bcl-6 (Phospho-Ser333) Antibody
Gene Names
BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Reactivity
Human (Identities = 100%, Positives = 100%);
Mouse (Identities = 100%, Positives = 100%);
Rat (Identities = 100%, Positives = 100%)
Applications
Western Blot
Purity
Affinity-purified
Pricing
product-image-AAA109366_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain using CSFR (Phospho-Tyr561) antibody. Western blot analysis of extracts from HepG2 cells, using CSFR (Phospho-Tyr561) antibody.)

CSFR, Antibody (Cat# AAA109366)

Full Name
CSFR (Phospho-Tyr561) Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109376_IF11.jpg IF (Immunofluorescence) (EGF +- Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.)

NPM, Antibody (Cat# AAA109376)

Full Name
NPM (Phospho-Thr199) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing

What Are Phospho Antibodies?


Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.


Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.


What Are The Key Applications of Phospho Antibodies?


1. Western Blotting


One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.


2. Staining of Fixed Cells (Immunocytochemistry)


Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.


3. Specificity Tests Using Peptides


In order to make sure that the antibody is only binding to the right target:


  • Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).

  • If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.

  • A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.

  • Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.


4. Genetic Confirmation


As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.


Why Buy Phospho Antibodies Through Us?


  • The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:

  • Standard methods and proper controls in all tests to ensure high quality.

  • These antibodies are tested and validated in different cell types and species.

  • High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.


FAQ


1. What Are Phospho-Specific Antibodies?


Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.


2. How to Detect Phosphorylated Proteins in a Western Blot?


To find out if a protein is phosphorylated using Western blot:


  • Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.

  • You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).


3. How to Choose the Best Antibody?


Here are some simple tips to help you pick the right antibody:


  • Know your target

  • Match your sample characteristics

  • Confirm the intended use is appropriate

  • Check “host” and “type”

  • Check the “quality” of the presented data/images

  • Appraise whether the available validation meets your needs


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