Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

Standard Curve (Sample)

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p-tau231 (Phospho Tau 231), ELISA Kit (Cat# AAA251000)

Full Name

Mouse p-tau231 (Phospho Tau 231) ELISA Kit

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Reactivity

Mouse

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

Standard Curve (Sample)

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p-tau181 (Phospho Tau 181), ELISA Kit (Cat# AAA251001)

Full Name

Mouse p-tau181 (Phospho Tau 181) ELISA Kit

Gene Names

Mapt; Tau; Mtapt; AI413597; AW045860

Reactivity

Mouse

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Myc(Phospho-Thr358) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Phospho-Thr358) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Myc(Phospho-Thr358) Antibody.)

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MYC, Polyclonal Antibody (Cat# AAA243262)

Full Name

Phospho-MYC (Thr358) Antibody

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser142) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Phospho-Ser142) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1(Phospho-Ser142) Antibody.)

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GATA1, Polyclonal Antibody (Cat# AAA243264)

Full Name

Phospho-GATA1 (Ser142) Antibody

Gene Names

GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; XLANP; XLTDA; GATA-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser106) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser106) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from MCF7 cells untreated or treated with EGF using Estrogen Receptor-a(Phospho-Ser106) Antibody.)

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ESR1, Polyclonal Antibody (Cat# AAA243265)

Full Name

Phospho-ESR1 (Ser106) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody.)

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EIF4E, Polyclonal Antibody (Cat# AAA243299)

Full Name

Phospho-EIF4E (Ser209) Antibody

Gene Names

EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase(Phospho-Thr202) Antibody.)

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MAPK3, Polyclonal Antibody (Cat# AAA243302)

Full Name

Phospho-MAPK3 (Thr202) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells treated with UV using Chk1 (Phospho-Ser296) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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CHEK1, Polyclonal Antibody (Cat# AAA243192)

Full Name

Phospho-CHEK1 (Ser296) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody. The lane on the left is treated with antigen-specific peptide.)

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MAP3K1, Polyclonal Antibody (Cat# AAA243207)

Full Name

Phospho-MAP3K1 (Thr1402) Antibody

Gene Names

MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.)

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TOB1, Polyclonal Antibody (Cat# AAA243229)

Full Name

Phospho-TOB1 (Ser164) Antibody

Gene Names

TOB1; TOB; TROB; APRO6; PIG49; TROB1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF7 cells using PTEN (phospho-Ser370) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (phospho-Ser370) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells using PTEN (phospho-Ser370) antibody.)

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PTEN, Polyclonal Antibody (Cat# AAA243337)

Full Name

Phospho-PTEN (Ser370) Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using ATRIP (Phospho-Ser224) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 292 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

ATRIP, Polyclonal Antibody (Cat# AAA243349)

Full Name

Phospho-ATRIP (Ser224) Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1(Phospho-Ser10) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody.)

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Histone H3.1, Polyclonal Antibody (Cat# AAA243366)

Full Name

Phospho-Histone H3.1 (Ser10) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using BCL-XL (Phospho-Ser62) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with EGF using BCL-XL (Phospho-Ser62) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-XL (phospho-Ser62) antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using BCL-XL (phospho-Ser62) antibody.)

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BCL2L1, Polyclonal Antibody (Cat# AAA243377)

Full Name

Phospho-BCL2L1 (Ser62) Antibody

Gene Names

BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody.)

Compare (5 max)

JUNB, Polyclonal Antibody (Cat# AAA243046)

Full Name

Phospho-JUNB (Ser259) Antibody

Gene Names

JUNB; AP-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Phospho-Tyr1175) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr1175) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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KDR, Polyclonal Antibody (Cat# AAA243071)

Full Name

Phospho-KDR (Tyr1175) Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Thr205) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

MAPT, Polyclonal Antibody (Cat# AAA243081)

Full Name

Phospho-MAPT (Thr205) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM(Phospho-Ser1981) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with UV using ATM(Phospho-Ser1981) Antibody)

Compare (5 max)

ATM, Polyclonal Antibody (Cat# AAA243087)

Full Name

Phospho-ATM (Ser1981) Antibody

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from A2780 cells, treated with calf intestinal phosphatase (CIP), using Chk1 (Phospho-Ser280) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1(Phospho-Ser280) Antibody.)

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CHEK1, Polyclonal Antibody (Cat# AAA243094)

Full Name

Phospho-CHEK1 (Ser280) Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Phospho-Ser498) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody.)

Compare (5 max)

HDAC5, Polyclonal Antibody (Cat# AAA243110)

Full Name

Phospho-HDAC5 (Ser498) Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1172) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1172) Antibody.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA243120)

Full Name

Phospho-EGFR (Tyr1172) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody.)

Compare (5 max)

STMN1, Polyclonal Antibody (Cat# AAA243122)

Full Name

Phospho-STMN1 (Ser25) Antibody

Gene Names

STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105(Phospho-Ser932) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF-a using NFkB-p105(Phospho-Ser932) Antibody.)

Compare (5 max)

NFKB1, Polyclonal Antibody (Cat# AAA243129)

Full Name

Phospho-NFKB1 (Ser932) Antibody

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from P19 cells, treated with calf intestinal phosphatase (CIP), using Interferon-a Receptor Type I Subunit I (phospho-Ser535/Ser539) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using Interferon-a Receptor Type I Subunit I (phospho-Ser535/Ser539) Antibody.)

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IFNAR1, Polyclonal Antibody (Cat# AAA243039)

Full Name

Phospho-IFNAR1 (Ser535/Ser539) Antibody

Gene Names

IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purification

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5 (T926) antibody.)

WB (Western Blot) (Western Blot analysis of COLO205 cells using Phospho-Eg5 (T926) Polyclonal Antibody)

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KIF11, Polyclonal Antibody (Cat# AAA236609)

Full Name

Phospho-KIF11 (T926) Antibody

Gene Names

KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5

Reactivity

Human

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody.)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody)

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BRAF, Polyclonal Antibody (Cat# AAA236627)

Full Name

Phospho-BRAF (S446) Antibody

Gene Names

BRAF; NS7; BRAF1; RAFB1; B-RAF1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-Synapsin I (S9) antibody.)

WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-Synapsin I (S9) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 3T3 cells using Phospho-Synapsin I (S9) Polyclonal Antibody)

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SYN1, Polyclonal Antibody (Cat# AAA236715)

Full Name

Phospho-SYN1 (S9) Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western blot analysis of K562 lysis using Phospho-p38 (T180/Y182) antibody.)

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-p38 (T180/Y182) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HEPG2 cells using Phospho-p38 (T180/Y182) Polyclonal Antibody)

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MAPK14, Polyclonal Antibody (Cat# AAA236716)

Full Name

Phospho-MAPK14 (T180/Y182) Antibody

Gene Names

MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of KB using Phospho-Akt (Y326) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Akt (Y326) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Akt (Y326) Polyclonal Antibody)

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AKT1, Polyclonal Antibody (Cat# AAA236717)

Full Name

Phospho-AKT1 (Y326) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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WB (Western Blot) (Western blot analysis of 3T3 lysis using Phospho-Fyn (Y530) antibody.)

WB (Western Blot) (Western blot analysis of 3T3 lysis using Phospho-Fyn (Y530) antibody.)

WB (Western Blot) (Western Blot analysis of LOVO+UV cells using Phospho-Fyn (Y530) Polyclonal Antibody)

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FYN, Polyclonal Antibody (Cat# AAA236770)

Full Name

Phospho-FYN (Y530) Antibody

Gene Names

FYN; SLK; SYN; p59-FYN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with CSB-RA019284A259phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA019284A259phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?Hela whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-RAF1 antibody at 1.18ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73 KDaObserved band size: 73 KDa)

Compare (5 max)

RAF1, Monoclonal Recombinant Antibody (Cat# AAA235584)

Full Name

Phospho-RAF1 (S259) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin ALane 1: Rabbit control IgG(1ug)instead of CSB-RA022812A727phHU in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA022812A727phHU(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg)Lane 3: 293 whole cell lysate treated with Calyculin A (20ug))

IHC (Immunohiostchemistry) (IHC image of CSB-RA022812A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?A549 whole cell lysate(treated with EGF or not)All lanes?Phospho-STAT3 antibody at 1.88ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 98 KDaObserved band size: 98 KDa)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA235592)

Full Name

Phospho-STAT3 (S727) Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (IHC image of CSB-RA022814A694phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?HepG2 whole cell lysate(treated with EGF or not)All lanes?Phospho-STAT5A antibody at 1.025ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

Compare (5 max)

STAT5A, Monoclonal Recombinant Antibody (Cat# AAA235593)

Full Name

Phospho-STAT5A (Y694) Antibody

Gene Names

STAT5A; MGF; STAT5

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (IHC image of CSB-RA026244A127phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?HepG2 whole cell lysate(treated with Calyculin A or not)All lanes?Phospho-YAP1 antibody at 0.83ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65 KDaObserved band size: 65 KDa)

Compare (5 max)

YAP1, Monoclonal Recombinant Antibody (Cat# AAA235599)

Full Name

Phospho-YAP1 (S127) Antibody

Gene Names

YAP1; YAP; YKI; COB1; YAP2; YAP65

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA614961A40phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

IHC (Immunohiostchemistry) (IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in?HepG2 whole cell lysate,293 whole cell lysate(treated with Calyculin A or EGF)All lanes?Phospho-NFE2L2 antibody at 0.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

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NFE2L2, Monoclonal Recombinant Antibody (Cat# AAA235600)

Full Name

Phospho-NFE2L2 (S40) Antibody

Gene Names

NFE2L2; NRF2; HEBP1; IMDDHH

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA618984A359phHU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA618984A359phHU(3ug)+ Hela whole cell lysate(1mg)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-RA618984A359phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in?HepG2 whole cell lysate(treated with EGF or not)All lanes?Phospho-RPS6KA1 antibody at 0.69ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

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RPS6KA1, Monoclonal Recombinant Antibody (Cat# AAA235603)

Full Name

Phospho-RPS6KA1 (T359+S363) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human

Applications

Immunoprecipitation, Immunofluorescence, Western Blot

Purity

Affinity-Chromatography

Pricing

$280/0.05 mL $375/0.1 mL $725/2x0.1 mL $1,060/3x0.1 mL $1,370/4x0.1 mL $1,680/5x0.1 mL

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-PLC gamma1 (Y783) Polyclonal Antibody)

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PLCG1, Polyclonal Antibody (Cat# AAA235651)

Full Name

Phospho-PLCG1 (Y783) Antibody

Gene Names

PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC A549 cells using Phospho-AP-1 (T93) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-AP-1 (T93) Polyclonal Antibody)

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JUN, Polyclonal Antibody (Cat# AAA235767)

Full Name

Phospho-JUN (T93) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-ATF-2 (S112) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-ATF-2 (S112) Polyclonal Antibody)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA235768)

Full Name

Phospho-ATF2 (S112) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-ATF-2 (T71) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HuvEc cells using Phospho-ATF-2 (T71) Polyclonal Antibody)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA235770)

Full Name

Phospho-ATF2 (T71) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-Bcl-2 (S70) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-Bcl-2 (S70) Polyclonal Antibody)

Compare (5 max)

BCL2, Polyclonal Antibody (Cat# AAA235771)

Full Name

Phospho-BCL2 (S70) Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of HeLa cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody)

Compare (5 max)

CAV1, Polyclonal Antibody (Cat# AAA235774)

Full Name

Phospho-CAV1 (Y14) Antibody

Gene Names

CAV1; CGL3; BSCL3; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of HELA cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody)

Compare (5 max)

KRT8, Polyclonal Antibody (Cat# AAA235785)

Full Name

Phospho-KRT8 (S73) Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-ERalpha (S118) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-ERalpha (S118) Polyclonal Antibody)

Compare (5 max)

ESR1, Polyclonal Antibody (Cat# AAA235790)

Full Name

Phospho-ESR1 (S118) Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of 3T3 HELA lysis using Phospho-FAK (Y861) antibody.)

WB (Western Blot) (Western Blot analysis of Jurkat cells using Phospho-FAK (Y861) Polyclonal Antibody)

Compare (5 max)

PTK2, Polyclonal Antibody (Cat# AAA235791)

Full Name

Phospho-PTK2 (Y861) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of NIH-3T3 cells using Phospho-FoxO1 (S319) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-FoxO1 (S319) Polyclonal Antibody)

Compare (5 max)

FOXO1, Polyclonal Antibody (Cat# AAA235792)

Full Name

Phospho-FOXO1 (S319) Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of Hela cells using Phospho-GSK3beta (S9) Polyclonal Antibody)

Compare (5 max)

GSK3B, Polyclonal Antibody (Cat# AAA235794)

Full Name

Phospho-GSK3B (S9) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of HELA SH-SY5Y 3T3 lysis using Phospho-HDAC4 (S632) antibody.)

WB (Western Blot) (Western Blot analysis of 293 cells using Phospho-HDAC4 (S632) Polyclonal Antibody)

Compare (5 max)

HDAC4, Polyclonal Antibody (Cat# AAA235795)

Full Name

Phospho-HDAC4 (S632) Antibody

Gene Names

HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC HY929 cells using Phospho-HDAC5 (S498) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-HDAC5 (S498) Polyclonal Antibody)

Compare (5 max)

HDAC5, Polyclonal Antibody (Cat# AAA235796)

Full Name

Phospho-HDAC5 (S498) Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of VEC 293 cells using Phospho-IRS-1 (S307) Polyclonal Antibody)

WB (Western Blot) (Western Blot analysis of VEC cells using Phospho-IRS-1 (S307) Polyclonal Antibody)

Compare (5 max)

IRS1, Polyclonal Antibody (Cat# AAA235803)

Full Name

Phospho-IRS1 (S307) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$190/0.05 mg $235/0.1 mg $460/2x0.1 mg $670/3x0.1 mg $870/4x0.1 mg $1,045/5x0.1 mg

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs