Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

WB (Western Blot) (Western blot analysis of CD32b (pY292) expression in MCF7 pervanadate-treated (A), mouse liver (B), PC12 pervanadate-treated (C) whole cell lysates.)

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CD32b (pY292), Polyclonal Antibody (Cat# AAA104790)

Full Name

Anti-CD32b (pY292) Phospho Antibody

Gene Names

FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Focal Adhesion Kinase (pY925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

WB (Western Blot) (Western blot analysis of Focal Adhesion Kinase (pY925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates.)

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Focal Adhesion Kinase (pY925), Polyclonal Antibody (Cat# AAA104886)

Full Name

Anti-Focal Adhesion Kinase (pY925) Phospho Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat, Chicken, Zebrafish

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of AMPK beta 1 (pS182) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of AMPK beta 1 (pS182) expression in A431 (A), NIH3T3 (B), PC12 (C) whole cell lysates.)

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AMPK beta 1 (pS182), Polyclonal Antibody (Cat# AAA104585)

Full Name

Anti-AMPK beta 1 (pS182) Phospho Antibody

Gene Names

PRKAB1; AMPK; HAMPKb

Reactivity

Human, Mouse, Rat, Bovine, Pig

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of p53 (pS315) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of p53 (pS315) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of p53 (pS315) expression in HeLa (A), A431 (B) whole cell lysates.)

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p53 (pS315), Polyclonal Antibody (Cat# AAA104599)

Full Name

Anti-p53 (pS315) Phospho Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Rat, Monkey

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using Mst1/2 (Phospho-Thr183) antibody. Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1/2 (Phospho-Thr183) antibody.)

Application Data (P-peptide-+)

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Mst1/2, Antibody (Cat# AAA109201)

Full Name

Mst1/2 (Phospho-Thr183) Antibody

Gene Names

STK4; KRS2; MST1; YSK3

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), using HSL (Phospho-Ser855/554) antibody (#A0074).)

IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using HSL (Phospho-Ser855/554) antibody (#A0074).)

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HSL, Antibody (Cat# AAA109214)

Full Name

HSL (Phospho-Ser855/554) Antibody

Gene Names

LIPE; HSL; LHS; AOMS4; FPLD6

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) antibody. MDM2 (Phospho-Ser186+Ser188) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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MDM2, Antibody (Cat# AAA109254)

Full Name

MDM2 (Phospho-Ser186+Ser188) Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

Immunofluorescence

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody. Western blot analysis of extracts from K562 cells, treated with serum (20%, 15mins), using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody.)

Application Data (P-peptide-+)

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Ephrin B1/B2/B3, Antibody (Cat# AAA109255)

Full Name

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody

Gene Names

EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using AurB/C (Phospho-Thr236/202) antibody. AurB/C (Phospho-Thr236/202) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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AurB/C, Antibody (Cat# AAA109561)

Full Name

AurB/C (Phospho-Thr236/202) Antibody

Gene Names

AURKC; AIE2; AIK3; ARK3; AurC; SPGF5; STK13; HEL-S-90; aurora-C

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody. Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using DDR1 (Phospho-Tyr513) antibody.)

Application Data (P-peptide-+)

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DDR1, Antibody (Cat# AAA109596)

Full Name

DDR1 (Phospho-Tyr513) Antibody

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody. HSF1 (Phospho-Thr142) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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HSF1, Antibody (Cat# AAA109380)

Full Name

HSF1 (Phospho-Thr142) Antibody

Gene Names

HSF1; HSTF1

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using INSR (Phospho-Thr1375) antibody.)

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IR, Antibody (Cat# AAA109382)

Full Name

IR (Phospho-Thr1375) Antibody

Gene Names

INSR; HHF5; CD220

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Phospho-Ser608) antibody.)

Application Data (P-peptide-+)

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Retinoblastoma, Antibody (Cat# AAA109680)

Full Name

Retinoblastoma (Phospho-Ser608) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (FITC) $580/0.1 mL (AF350) $580/0.1 mL (AF488) $580/0.1 mL (AF555) $580/0.1 mL (AF568) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (Western blot analysis using CDC2 (Phospho-Thr161) Antibody: Line1: The extracts from COLO cells using preincubated with synthesized phosphopeptide; Line2: The extracts from COLO cells. Line3: The extracts from K562 cells.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded breast carcinoma, using CDC2(Phospho-Thr161) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.)

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CDC2, Antibody (Cat# AAA109717)

Full Name

CDC2 (Phospho-Thr161) Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser366) antibody. Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using p53 (Phospho-Ser366) antibody.)

Application Data (P-peptide-+)

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p53, Antibody (Cat# AAA109761)

Full Name

p53 (Phospho-Ser366) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using AKT1/2/3 (Phospho-Tyr315/316/312) antibody. AKT1/2/3 (Phospho-Tyr315/316/312) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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AKT1/2/3, Antibody (Cat# AAA109829)

Full Name

AKT1/2/3 (Phospho-Tyr315/316/312) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using DARPP-32 (Phospho-Thr34) antibody. Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using DARPP-32 (Phospho-Thr34) antibody.)

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DARPP-32, Antibody (Cat# AAA109607)

Full Name

DARPP-32 (Phospho-Thr34) Antibody

Gene Names

PPP1R1B; DARPP32; DARPP-32

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr757) antibody. Trk A (Phospho-Tyr757) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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Trk A, Antibody (Cat# AAA109613)

Full Name

Trk A (Phospho-Tyr757) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP) $710/0.1 mL (PE)

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IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using eIF4B (Phospho-Ser422) antibody. Western blot analysis of extracts from NIH/3T3cells, using eIF4B (Phospho-Ser422) antibody.)

Application Data (P-peptide-+)

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eIF4B, Antibody (Cat# AAA109672)

Full Name

eIF4B (Phospho-Ser422) Antibody

Gene Names

EIF4B; EIF-4B; PRO1843

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HP1alpha (Phospho-Ser92) antibody. HP1alpha (Phospho-Ser92) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Application Data (P-peptide-+)

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HP1alpha, Antibody (Cat# AAA109307)

Full Name

HP1alpha (Phospho-Ser92) Antibody

Gene Names

CBX5; HP1; HP1A; HEL25

Reactivity

Human

Applications

Immunohistochemistry

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

WB (Western Blot) (Western blot analysis of extracts from mouse liver cells, using Akt (Phospho-Tyr326) antibody.)

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Akt, Antibody (Cat# AAA109498)

Full Name

Akt (Phospho-Tyr326) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

Standard Curve (Sample)

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Phospho Fructo Kinase 1 (PFK-1), ELISA Kit (Cat# AAA122602)

Full Name

Mouse Phospho Fructo Kinase 1 (PFK-1) ELISA Kit

Reactivity

Mouse

Pricing

$455/48-Strip-Wells $670/96-Strip-Wells $1,305/2x96-Strip-Wells $2,860/5x96-Strip-Wells $5,625/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-STAT3 (Y705), p-STAT3, ELISA Kit (Cat# AAA123890)

Full Name

Mouse Phospho-STAT3 (Y705), p-STAT3 ELISA Kit

Reactivity

Mouse

Pricing

$455/48-Strip-Wells $670/96-Strip-Wells $1,305/2x96-Strip-Wells $2,860/5x96-Strip-Wells $5,625/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-C-Jun N-Terminal Kinases (p-JNK), ELISA Kit (Cat# AAA209002)

Full Name

Rat Phospho-C-Jun N-Terminal Kinases (p-JNK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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NFKBIA, ELISA Kit (Cat# AAA209003)

Full Name

Rat Phospho-NFKBIA (Ser32/36) ELISA Kit

Gene Names

NFKBIB; IKBB; TRIP9

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-P38 Mitogen Activated Protein Kinase (p-P38MAPK), ELISA Kit (Cat# AAA209004)

Full Name

Rat Phospho-P38 Mitogen Activated Protein Kinase (p-P38MAPK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Extracellular Signal-Regulated Kinase (PERK), ELISA Kit (Cat# AAA209009)

Full Name

Rat Phospho-Extracellular Signal-Regulated Kinase (PERK) ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Disabled Homolog 1, ELISA Kit (Cat# AAA209999)

Full Name

Rat Phospho-Disabled Homolog 1 ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

Standard Curve (Sample)

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Phospho-Acetyl CoA Carboxylase, ELISA Kit (Cat# AAA209875)

Full Name

Rat Phospho-Acetyl CoA Carboxylase ELISA Kit

Reactivity

Rat

Pricing

$665/48-Strip-Wells $895/96-Strip-Wells $1,740/2x96-Strip-Wells $4,020/5x96-Strip-Wells $7,890/10x96-Strip-Wells

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WB (Western Blot) (Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 ug) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. AAA211140) used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)

IHC (Immunohiostchemistry) (Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. AAA211140 Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [AAA211140]in a 150 ul volume. DNA content was simultaneously analyzed via PI stain.)

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MCM2, Polyclonal Antibody (Cat# AAA211140)

Full Name

Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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WB (Western Blot) (Detection of mouse Phospho MCM2 (Ser108) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody (AAA210759 lot 5) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-MCM2 antibody .)

WB (Western Blot) (Detection of human Phospho MCM2 (Ser108) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser108) antibody (AAA210759 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-MCM2 antibody .)

IP (Immunoprecipitation) (Detection of human Phospho MCM2 (Ser108) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells treated with etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-MCM2 antibody used for IP at 6 ug per reaction. Following immunoprecipitation, the IP reaction was treated with phosphatases (+) or mock treated (-). For blotting immunoprecipitated Phospho MCM2 (Ser108), AAA210759 lot 5 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-MCM2 antibody .)

IHC (Immunohiostchemistry) (Detection of mouse Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) (Cat. No. AAA210759 Lot5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) (Cat. No. AAA210759 Lot5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA210759)

Full Name

Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (Detection of human Phospho Histone H3 (pS10) by western blot. A-549, HeLa, K-562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degree C, 5% CO? for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody AAA211652 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Cat. No. Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemisry) (Detection of human and mouse Histone H3 (S10) by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10)(Cat. No. AAA211652 Lot1) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB)

ICC (Immunocytochemistry) (Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA211652 Lot1) used at a dilution of 1:2,500 (0.08ug/ml). Detection: Green fluorescent goat antirabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)

FCM/FACS (Flow Cytometry) (Detection of Phospho Histone H3 (pS10). Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 x 10^6 cells were stained with 0.4 ug anti-phospho Histone H3 (S10) [AAA211652] in a 150 ul reaction.)

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Histone H3, Polyclonal Antibody (Cat# AAA211652)

Full Name

Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $635/0.02 mg $940/5x0.002 mg $1,230/2x0.02 mg $1,800/3x0.02 mg $2,335/4x0.02 mg $2,630/5x0.02 mg

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IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: ZAP70 antibody was used at 3 ug/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated. For blotting immunoprecipitated Phospho ZAP70, Phospho ZAP70 (Y319) antibody AAA211718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Phospho ZAP70 antibodies AAA211718 (phosphorylation at Y319), (phosphorylation at Y493), and (phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, ZAP70 antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 min.)

FCM/FACS (Flow Cytometry) (Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10^6 cells stained with 1.0 ug/ml Phospho Zap70 (Y319) antibody AAA211718 in a 150 ul volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), anti-phospho Zap70 (Y319) in H2O2 treated (blue).)

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ZAP70, Polyclonal Antibody (Cat# AAA211718)

Full Name

Rabbit anti-Phospho ZAP70 (Y319) Antibody, Affinity Purified

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S536) antibody AAA211745 (lot AAA211745-12) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower panel: Western blot for total RelA using rabbit anti-RelA antibody at 0.1 ug/ml with exposure time of 30 seconds.)

WB (Western Blot) (Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-), and treated with Phosphatase (+) or mock treated (-). Antibodies: Rabbit anti-RelA antibody used for IP at 6 ug/mg lysate. Affinity purified rabbit anti-Phospho RelA (S536) antibody AAA211745 (lot AAA211745-12 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel: Western blot for total RelA using affinity purified rabbit anti-RelA antibody at 0.1 ug/ml with exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human Phospho RelA (S536) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S536) (Cat. No. AAA211745 Lot12) used at a dilution of 1:200 (1ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).)

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RelA, Polyclonal Antibody (Cat# AAA211745)

Full Name

Rabbit anti-Phospho RelA (S536) Antibody, Affinity Purified

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $655/0.02 mg $940/5x0.002 mg $1,270/2x0.02 mg $1,860/3x0.02 mg $2,410/4x0.02 mg $2,715/5x0.02 mg

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WB (Western Blot) (Detection of human Phospho RelA (S529) by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RelA (S529) antibody AAA211746 (lot AAA211746-1) used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of human Phospho RelA (S529) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: RelA was immunoprecipitated with affinity purified rabbit anti-RelA antibody Immunoprecipitated RelA was treated with phosphatase or mock treated. Affinity purified rabbit anti-Phospho RelA (S529) antibody AAA211746 (lot AAA211746-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

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RelA, Polyclonal Antibody (Cat# AAA211746)

Full Name

Rabbit anti-Phospho RelA (S529) Antibody, Affinity Purified

Reactivity

Human

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $655/0.1 mg $940/5x0.01 mg $1,270/2x0.1 mg $1,860/3x0.1 mg $2,410/4x0.1 mg $2,715/5x0.1 mg

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WB (Western Blot) (Detection of human Chk1, Phospho (S317) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), etoposide (100uM for 4 hours), or untreated. Antibodies: Affinity purified rabbit anti-Chk1, Phospho (S317) antibody AAA212853 (lot AAA212853-1) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistochemistry) (Detection of human Chk1, Phospho (S317) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Chk1, Phospho (S317) (Cat. No. AAA212853 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)

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Chk1, Polyclonal Antibody (Cat# AAA212853)

Full Name

Rabbit anti-Phospho Chk1 (S317) Antibody, Affinity Purified

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $655/0.1 mg $940/5x0.01 mg $1,270/2x0.1 mg $1,860/3x0.1 mg $2,410/4x0.1 mg $2,715/5x0.1 mg

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WB (Western Blot) (Detection of human Phospho-RPA32 (S4/8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-RPA32 (S4/8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin antibody .)

IP (Immunoprecipitation) (Detection of human Phospho RPA32 (S4/8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Phospho RPA32 (S4/8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533 lot 3) used for IP at 6 ul/mg lysate. Phospho RPA32 (S4/8) was also immunoprecipitated by a previous lot of this antibody (AAA213533 lot 2) and by rabbit anti-RPA32 antibody . For blotting immunoprecipitated Phospho RPA32 (S4/8), AAA213533 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Total RPA32 detected by rabbit anti-RPA32 .)

ICC (Immunocytochemistry) (Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left). Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (AAA213533) used at 1:5,000. Detection: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)

ICC (Immunocytochemistry) (Detection of human Phospho-RPA32 (S4/S8) in FFPE etoposide-treated HeLa cells, mock CIP (left) and CIP (right). Antibody: Rabbit anti-phospho RPA32 (S4/S8) [BL-165-5F1] (AAA213533). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM/FACS (Flow Cytometry) (Detection of human phospho RPA32 (shaded) in etoposide treated HEK293T cells (right) and untreated HEK293T cells (right) by flow cytometry. Antibody: Rabbit anti-phospho RPA32 recombinant monoclonal [BL-165-5F1] (AAA213533) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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RPA32, Monoclonal Recombinant Antibody (Cat# AAA213533)

Full Name

Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Immunocytochemistry, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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IHC (Immunohistochemisry) (Detection of mouse phospho-MCM2 (Ser 41) by immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho-MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:100. Detection: DAB)

IHC (Immunohiostchemistry) (Detection of human Phospho MCM2 (Ser 41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) or CIP phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human phospho-MCM2 (Ser 41) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Phospho-MCM2 (Ser 41) (Cat. No. AAA213731) used at a dilution of 1:100. Detection: DAB)

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MCM2, Polyclonal Antibody (Cat# AAA213731)

Full Name

Rabbit anti-Phospho MCM2 (S41) IHC Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.001 mg $665/0.01 mg $940/5x0.001 mg $1,295/2x0.01 mg $1,890/3x0.01 mg $2,450/4x0.01 mg $2,780/5x0.01 mg

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IHC (Immunohiostchemistry) (Detection of mouse Phospho SMC1 (Ser 966) by immunohistochemistry. Samples: FFPE sections of mouse squamous cell carcinoma. Mock treatment (left panel) or lambda phosphatase-treatment (right panel). Antibody: Affinity purified rabbit anti-Phospho-SMC1 (Ser 966) (Cat. No. AAA213732) used at a dilution of 1:250. Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho SMC1 (Ser 966) by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment (left panel) or lambda phosphatase-treatment (right panel). Antibody: Affinity purified rabbit anti-Phospho-SMC1 (Ser 966) (Cat. No. AAA213732) used at a dilution of 1:250. Detection: DAB)

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SMC1, Polyclonal Antibody (Cat# AAA213732)

Full Name

Rabbit anti-Phospho SMC1 (S966) IHC Antibody, Affinity Purified

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.0025 mg $665/8 mL $940/5x0.0025 mg $1,295/2x8 mL $1,890/3x8 mL $2,450/4x8 mL $2,780/5x0.025 mg

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WB (Western Blot) (Detection of human Phospho STAT3 (S727) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (IFNalpha), Jurkat, Jurkat (IFNalpha), and NCI-H226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho STAT3 (S727) recombinant monoclonal antibody (AAA213617 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human Phospho STAT3 (S727) by western blot of immunoprecipitates from HeLa cells treated with IFN-alpha (100 ng/mL). IPs were treated (+) or mock treated (-) with phosphatase. Antibodies: Rabbit anti-STAT3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blotting immunoprecipitated Phospho STAT3 (S727), AAA213617 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Total STAT3 antibody .)

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STAT3, Monoclonal Recombinant Antibody (Cat# AAA213617)

Full Name

Rabbit anti-Phospho STAT3 (S727) Recombinant Monoclonal Antibody [BLR160J]

Gene Names

STAT3; APRF; HIES

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$265/0.02 mL $455/0.1 mL $890/2x0.1 mL $955/5x0.02 mL $1,295/3x0.1 mL $1,680/4x0.1 mL $1,825/5x0.1 mL

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IHC (Immunohiostchemistry) (Detection of human Phospho Histone H3 (S10) by immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA213763) used at a dilution of 1:250. Detection: DAB)

ICC (Immunocytochemistry) (Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated cells (left) or calf intestinal phosphatase-treated cells (right) immunostained for Phospho Histone H3 (S10). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. AAA213763) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)

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Histone H3, Polyclonal Antibody (Cat# AAA213763)

Full Name

Rabbit anti-Phospho Histone H3 (S10) IHC Antibody, Affinity Purified

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$255/0.002 mg $500/2x0.002 mg $730/3x0.002 mg $945/4x0.002 mg $2,780/5x0.002 mg

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WB (Western Blot) (Western blot analysis of Phospho-Creb(Ser133) expression in HeLa cell lysates treated with Calyculin A (AAA124530).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CREB1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody(AAA124530)CREB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CREB1 Antibody (AAA124530)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Creb, Monoclonal Antibody (Cat# AAA124530)

Full Name

Anti-Phospho-Creb (S133) Rabbit Monoclonal Antibody

Gene Names

CREB1; CREB; CREB-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GSK3 beta (phospho S9) expression in 293T cell lysates, treated with Calyculin A (AAA124533).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GSK3B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody(AAA124533)GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (AAA124533)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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GSK3 beta, Monoclonal Antibody (Cat# AAA124533)

Full Name

Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin (AAA124534).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATF2)

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ATF2, Monoclonal Antibody (Cat# AAA124534)

Full Name

Anti-Phospho-ATF2 (T71) Rabbit Monoclonal Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-EIF2S1(Ser51) expression in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates (AAA124539).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF2S1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-EIF2S1 (S51) Antibody(AAA124539)EIF2S1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EIF2S1 Antibody (AAA124539)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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EIF2S1, Monoclonal Antibody (Cat# AAA124539)

Full Name

Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin (AAA124521).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATM)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody(AAA124521)ATM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATM Antibody (AAA124521)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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ATM, Monoclonal Antibody (Cat# AAA124521)

Full Name

Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody

Gene Names

ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Rb (phospho S807) expression in K562 cell lysate (AAA124525).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RB1)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Rb (S807) Antibody (AAA124525)RB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RB1 Antibody (AAA124525)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

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Rb, Monoclonal Antibody (Cat# AAA124525)

Full Name

Anti-Phospho-Rb (S807) Rabbit Monoclonal Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells treated with TGF? , using Phospho-Smad3 (S423 + S425) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.)

WB (Western Blot) (Western blot analysis of Phospho-Smad3 (S423/S425) expression in PC-12 cell lysate treated with 1% BSA.)

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Smad3, Monoclonal Antibody (Cat# AAA124526)

Full Name

Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

Standard Curve (Sample)

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p-tau231 (Phospho Tau 231), ELISA Kit (Cat# AAA252650)

Full Name

Human p-tau231 (Phospho Tau 231) QuickTest ELISA Kit

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human

Pricing

$415/48-Strip-Wells $560/96-Strip-Wells $1,080/2x96-Strip-Wells $2,465/5x96-Strip-Wells $4,705/10x96-Strip-Wells

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs