Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PrP in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PrP in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PrP antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PrP on different lysates using anti-PrP antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse brain)

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Prion Protein (PrP), Monoclonal Antibody (Cat# AAA30117)

Full Name

Prion Protein (PrP) Antibody

Gene Names

PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Wei. Yuzhen. et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.)

Application Data (Wang. Yingying. et al. "p75NTR?/? mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/??catenin pathway." Cell proliferation 53.4 (2020): e12800.)

Application Data (Ho. Philip Wing-Lok. et al. "Age-dependent accumulation of oligomeric SNCA/?-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.)

Application Data (Du. Shaobo. et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells againstultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).)

Application Data (Cheng. Xiaocheng. et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-?/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.)

Application Data (Chen. Luchao. et al. "Effects of purified Omphalia lapidescens protein on metastasis. cell cycle. apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.)

WB (Western Blot) (Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4 degree over night. secondary antibody)

Application Data (The picture was kindly provided by our customer)

Application Data (The picture was kindly provided by our customer)

Application Data (The picture was kindly provided by our customer)

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GAPDH, Monoclonal Antibody (Cat# AAA30794)

Full Name

GAPDH (PTR2304) Monoclonal Antibody

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovin

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

Protein G

Pricing

$320/0.1 mL $870/0.5 mL $1,380/1 mL $2,665/2x1 mL $3,875/3x1 mL $5,020/4x1 mL $5,865/5x1 mL

Application Data

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Fumonisin, Monoclonal Antibody (Cat# AAA11720)

Full Name

Fumonisin Monoclonal Antibody

Applications

ELISA

Pricing

$675/0.1 mg $1,290/2x0.1 mg $1,875/3x0.1 mg $2,420/4x0.1 mg $2,760/5x0.1 mg

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Application Data (Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)

Application Data (Published customer image: B. abortus infection does not induce IL-17 or IFN- gamma production by gamma delta T cells. A. Splenocytes from na¯ve or B. abortus-infected mice (7 dpi) were stimulated overnight with PMA/Ionomycin and brefeldin A was added for the last 3 h of culture. Following surface staining, cells were permeabilized and stained for intracellular IL-17 or IFN-?. Top panel, the proportion of IL-17 producing gamma delta T cells was determined following gating on lymphocytes. Second panel from top, cells were gated on CD4+ (CD3+) T cells and assayed for IL-17 production. Third panel from top, cells were gated on gamma delta T cells (CD3+/TCR gamma delta +) and assayed for IFN- gamma production. Bottom panel, cells were gated on CD4+ (CD3+) T cells and assayed for IFN- gamma production. Depicted is the mean +/- SD of 5 mice/group and is representative of two independent experiments. B. gamma delta T cells were sorted from na¯ve or B. abortus-infected (7 dpi) mice and stimulated for 72 h with PMA/Ionomycin. Cytokine levels in supernatant were determined by ELISA. Depicted is the mean +/- SD of triplicate wells. *P)

Application Data (Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P)

Application Data (Published customer image: gamma delta T cells require TNF-alpha to protect against B. abortus infection. C57BL/6 mice treated with anti-TCR gamma delta mAb or hamster IgG on day -1 and day 3 post-infection with 5x104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-alpha on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean +/- SEM of 10 mice/group is depicted; *P)

Application Data (Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 ug/ml polyI:C, 0.5 ug/ml R-848 or 5 ug/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.)

Application Data (Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)

Application Data (Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.)

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IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)

Full Name

MOUSE ANTI BOVINE INTERFERON GAMMA

Reactivity

Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.

Applications

Flow Cytometry

Pricing

$860/0.5 mg $1,650/2x0.5 mg $2,395/3x0.5 mg $3,095/4x0.5 mg $3,545/5x0.5 mg

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Application Data (Published customer image: F4/80 and CD11b+ cells infiltrating the iris after the intracameral injection of antigen also express Ly6C, 7/4 (Ly 6B) and CD45. Preparations of na¯ve iris cells and cells recovered after an intracameral injection of antigen were stained with anti- F4/80 and (A) Ly6C and 7/4 (B) show a characteristic CD45 peak C) Ly6Glo or negative, CD115, CD49b+, CD62Llo. The figures are representative of 2 experiments. All the isotype controls in the histograms are shown as shaded.From: Pais R, Bhowmick S, Chattopadhyay S, Lemire Y, Sharafieh R, et al. (2012) An Intracameral Injection of Antigen Induces In Situ Chemokines and Cytokines Required for the Generation of Circulating Immunoregulatory Monocytes. PLoS ONE 7(8): e43182.)

Application Data (Published customer image: Renal inflammatory changes in Ntn1+/- mice following ischemia. Ntn1+/- mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A -D) Neutrophil staining. Arrows indicate neutrophils (magnification 400x). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO). (F) TNF-a and (G) interleukin-6 (IL-6) and (H) interleukin-10 (IL-10) were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to beta-actin and are expressed as fold change compared to sham-operated animals without ischemia (-I). Data are representative of four to six independent experiments for each experimental condition (mean +/- SD).From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. (2011) Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.)

Application Data (Published customer image: 166 mM H2O2 increased neutrophil infiltration in day 1 and 6 wounds. Fluorescence intensity of the neodermis was quantified using ImageJ. The area quantified is outlined with the dashed line. Results shown are mean +/- S.E.M, n = 6-7. A representative section from each treatment is shown. ES - Eschar; HE - Hyper-proliferating epidermis; ND - neodermis.*p)

Application Data (Published customer image: Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.From: Tian Y, Linden J, French BA, Yang Z (2014) Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9(12): e114375.)

Application Data (Published customer image: Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 um in length. All micrographs were taken using 200x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

Application Data (Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

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Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$660/0.25 mg $1,255/2x0.25 mg $1,825/3x0.25 mg $2,365/4x0.25 mg $2,650/5x0.25 mg

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Application Data (Published originator image Intact spleen segments from infected animals were fixed in paraformaldehyde, embedded in OCT, sectioned, and stained with various antibodies for the infiltration of macrophages (FA-11), polymorphonuclear neutrophils, and activated monocytes (7/4Ag) and activated macrophages (MHC-II), as described in the text. All data are representative of at least three individual experiments.From: Plüddemann A, Hoe JC, Makepeace K, Moxon ER, Gordon S (2009) The Macrophage Scavenger Receptor A Is Host-Protective in Experimental Meningococcal Septicaemia. PLoS Pathog 5(2): e1000297.)

Application Data (Published customer image: F4/80 and CD11b+ cells infiltrating the iris after the intracameral injection of antigen also express Ly6C, 7/4 (Ly 6B) and CD45. Preparations of na¯ve iris cells and cells recovered after an intracameral injection of antigen were stained with anti- F4/80 and (A) Ly6C and 7/4 (B) show a characteristic CD45 peak C) Ly6Glo or negative, CD115, CD49b+, CD62Llo. The figures are representative of 2 experiments. All the isotype controls in the histograms are shown as shaded.From: Pais R, Bhowmick S, Chattopadhyay S, Lemire Y, Sharafieh R, et al. (2012) An Intracameral Injection of Antigen Induces In Situ Chemokines and Cytokines Required for the Generation of Circulating Immunoregulatory Monocytes. PLoS ONE 7(8): e43182.)

Application Data (Published customer image: Renal inflammatory changes in Ntn1+/- mice following ischemia. Ntn1+/- mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A -D) Neutrophil staining. Arrows indicate neutrophils (magnification 400x). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO). (F) TNF-a and (G) interleukin-6 (IL-6) and (H) interleukin-10 (IL-10) were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to beta-actin and are expressed as fold change compared to sham-operated animals without ischemia (-I). Data are representative of four to six independent experiments for each experimental condition (mean +/- SD).From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. (2011) Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.)

Application Data (Published customer image: 166 mM H2O2 increased neutrophil infiltration in day 1 and 6 wounds. Fluorescence intensity of the neodermis was quantified using ImageJ. The area quantified is outlined with the dashed line. Results shown are mean +/- S.E.M, n = 6-7. A representative section from each treatment is shown. ES - Eschar; HE - Hyper-proliferating epidermis; ND - neodermis.*p)

Application Data (Published customer image: Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.From: Tian Y, Linden J, French BA, Yang Z (2014) Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9(12): e114375.)

Application Data (Published customer image: Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 um in length. All micrographs were taken using 200x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

Application Data (Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

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Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE

Applications

Flow Cytometry

Pricing

$1,185/500 Tests $2,280/2x500 Tests $3,325/3x500 Tests $4,305/4x500 Tests $5,025/5x500 Tests

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Staining Procedure (1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)

Presentation (Anti-Podoplanin/D2-40 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.)

IHC (Immunohistochemistry) (Inset: IHC of Podoplanin/D2-40 on a FFPE Tonsil Tissue)

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Podoplanin/D2-40, Monoclonal Antibody (Cat# AAA13558)

Full Name

Podoplanin/D2-40

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

$240/5 Slides $340/0.1 mL (Concentrate) $405/3 mL (RTU) $650/7 mL (RTU) $710/0.5 mL (Concentrate) $1,070/15 mL (RTU) $1,155/1 mL (Concentrate) $2,225/2x1 mL (Concentrate) $3,235/3x1 mL (Concentrate) $4,195/4x1 mL (Concentrate) $4,505/5x15 mL (RTU) $4,905/5x1 mL (Concentrate)

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IF (Immunofluorescence) (Figure 6. immunofluorescence staining of human colon epithelium.)

IF (Immunofluorescence) (Figure 5. immunofluorescence staining of 1 month old zebrafish embryo.)

IF (Immunofluorescence) (Figure 4. immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.)

IHC (Immunohistochemistry) (Figure 3. immunohistochemistry on frozen section of swine liver hepatocytes.)

IHC (Immunohistochemistry) (Figure 2. immunohistochemistry on frozen section of human colon.)

IHC (Immunohistochemistry) (Figure 1. Immunohistochemistry on frozen section of human kidney epithelium.)

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CytokeRatin 18 / KeRatin K18, Monoclonal Antibody (Cat# AAA14563)

Full Name

Mouse anti CytokeRatin 18 / KeRatin K18

Gene Names

KRT18; K18; CYK18

Reactivity

Canine, Chicken,Dog,Hamster, Human, Mouse, Rabbit, Rat, Swine, Zebrafish

Applications

Flow Cytometry, Immunoblot, Immunocytochemistry, Immunohistochemistry, Western Blot

Pricing

$645/0.1 mg $1,230/2x0.1 mg $1,780/3x0.1 mg $2,305/4x0.1 mg $2,590/5x0.1 mg

Structure (Typical Structure of an Antibody)

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Human IgG1 Isotype Control, Monoclonal Isotype Control (Cat# AAA14850)

Full Name

Human IgG1 Isotype Control

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry

Purity

Purified by fractionation, ion exchange and affinity chromatography; is or >95% (SDS-PAGE).

Pricing

$685/0.1 mg $1,310/2x0.1 mg $1,905/3x0.1 mg $2,460/4x0.1 mg $2,800/5x0.1 mg

Application Data

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CD29, Monoclonal Antibody (Cat# AAA14855)

Full Name

anti-human CD29-Purified Preservative Free

Gene Names

ITGB1; CD29

Reactivity

Human, Pig, Mouse

Applications

Flow Cytometry, Western Blot

Purity

Purified Preservative Free

Pricing

$425/0.1 mg $810/2x0.1 mg $1,160/3x0.1 mg $1,495/4x0.1 mg $1,620/5x0.1 mg

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557). Confirmation of Integrity and Purity of Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) followed by goat anti-Mouse IgG-CF488 (green). Membrane stained with Phalloidin (red).)

FCM (Flow Cytometry) (Flow Cytometric Analysis of MCF-7 cells using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).)

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Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA23915)

Full Name

Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)

FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)

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STIP1, Monoclonal Antibody (Cat# AAA26275)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 (Cat # L008V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9 Western Blot analysis of SMAD1 expression in HeLa (Cat # L013V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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SMAD1, Monoclonal Antibody (Cat# AAA26459)

Full Name

SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (HRP)

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of HeLa cells using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (A22421-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of C6 cells using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (A22421-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse colon tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (A22421-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of various lysates using Cation-independent M6PR (IGF2R) Rabbit PolymAb® (AAA28549) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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Cation-independent M6PR (IGF2R), Monoclonal Antibody (Cat# AAA28549)

Full Name

Cation-independent M6PR (IGF2R) Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Fig.3. Immunofluorescence analysis of mouse liver tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

IF (Immunofluorescence) (Fig.2. Immunofluorescence analysis of human colon cancer tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

WB (Western Blot) (Fig.1. Western blot analysis of 1) Hela, 2) rat brian tissue, 3) mouse brain tissue, diluted at 1:5000.)

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alpha-Tubulin, Monoclonal Antibody (Cat# AAA31544)

Full Name

Anti-alpha-Tubulin Monoclonal Antibody (3G5)

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Pricing

$185/0.05 mL $305/0.2 mL $565/2x0.2 mL $700/5x0.2 mL $810/3x0.2 mL $1,035/4x0.2 mL

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Application Data (Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)

Application Data (Staining of frozen rat spleen with Mouse anti Rat CD4)

Application Data (Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. High power)

Application Data (Staining of rat splenocytes with Mouse anti Rat CD4)

Application Data (Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. Low power)

Application Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD4)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)

Application Data (Published customer image: Dynamics of T-regulatory cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 um.From: Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)

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CD4, Monoclonal Antibody (Cat# AAA11995)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$460/0.1 mg $875/2x0.1 mg $1,260/3x0.1 mg $1,635/4x0.1 mg $1,760/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescent staining of PFA-fixed Raji cells. CD79a Mouse Monoclonal Antibody (JCB117) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.)

FCM (Flow Cytometry) (Flow Cytometric Analysis of Raji cells. CD79a Mouse Monoclonal Antibody (JCB117) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)

WB (Western Blot) (Western Blot Analysis of Raji cell lysate using CD79a Mouse Monoclonal Antibody (JCB117).)

WB (Western Blot) (Western Blot Analysis of human Raji cell lysate using CD79a Mouse Monoclonal Antibody (JCB117).)

SDS-PAGE (SDS-PAGE Analysis Purified CD79a Mouse Monoclonal Antibody (JCB117). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human tonsil stained with CD79a Mouse Monoclonal Antibody (JCB117).)

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CD79a, Monoclonal Antibody (Cat# AAA23891)

Full Name

CD79a (B-Cell Marker) Mouse Monoclonal Antibody

Gene Names

CD79A; IGA; MB-1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (human heart tissue were subjected to SDS PAGE followed by western blot with AAA27493 (RENALASE antibody) at dilution of 1:2000)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using AAA27493 (RENALASE Antibody) at dilution of 1:100)

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RENALASE, Monoclonal Antibody (Cat# AAA27493)

Full Name

RENALASE Mouse Monoclonal

Applications

Western Blot, Immunohistochemistry

Purity

Purity: >=95% as determined by SDS-PAGE
Purification: Protein A+G purification

Pricing

$380/0.1 mg $710/2x0.1 mg $1,020/3x0.1 mg $1,325/4x0.1 mg $1,405/5x0.1 mg

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Application Data (Published customer image: Characterization of high dose zymosan peritonitis. A) Representative flow-cytometric density plots showing the recruitment of inflammatory cells and the clearance of fluorescent (FITC)-labelled zymosan particles. The examples shown are taken from 18 hours after the administration of zymosan. Cells were identified as described in the methods. The panel on the left shows the gating of Ly-6B+ cells, which includes (right) Ly-6Ghigh neutrophils and Ly-6G- monocytes and M˜. Both neutrophils and monocytes/M˜ exhibit association with FITC-labeled zymosan at this time point (right panel). B) Graphical representation of the number of neutrophils (left) and all types of monocyte (Mo) and M˜ combined (right) in the peritoneal cavity before and after acute zymosan peritonitis. n = 3 129S6/SvEv mice per group and is representative of 2 independent experiments. Data is shown as mean+/-SEM and cells were isolated from na¯ve animals (0 hours), and challenged mice 4, 18, 72 and 168 hours after induction of peritonitis. C) Graphical representation of the percentage of neutrophils and Mo/M˜ present that are associated with zymosan, scored by flow-cytometry as indicated in (A) above. D) Photomicrograph, which is representative of neutrophils associated with zymosan in a 4 hour inflammatory infiltrate. Those neutrophils that are associated tend to have multiple particles, but only represent a minority of the total neutrophils present at this time (see (C) above).From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

Application Data (Published originator image Intact spleen segments from infected animals were fixed in paraformaldehyde, embedded in OCT, sectioned, and stained with various antibodies for the infiltration of macrophages (FA-11), polymorphonuclear neutrophils, and activated monocytes (7/4Ag) and activated macrophages (MHC-II), as described in the text. All data are representative of at least three individual experiments.From: Plüddemann A, Hoe JC, Makepeace K, Moxon ER, Gordon S (2009) The Macrophage Scavenger Receptor A Is Host-Protective in Experimental Meningococcal Septicaemia. PLoS Pathog 5(2): e1000297.)

Application Data (Published customer image: F4/80 and CD11b+ cells infiltrating the iris after the intracameral injection of antigen also express Ly6C, 7/4 (Ly 6B) and CD45. Preparations of na¯ve iris cells and cells recovered after an intracameral injection of antigen were stained with anti- F4/80 and (A) Ly6C and 7/4 (B) show a characteristic CD45 peak C) Ly6Glo or negative, CD115, CD49b+, CD62Llo. The figures are representative of 2 experiments. All the isotype controls in the histograms are shown as shaded.From: Pais R, Bhowmick S, Chattopadhyay S, Lemire Y, Sharafieh R, et al. (2012) An Intracameral Injection of Antigen Induces In Situ Chemokines and Cytokines Required for the Generation of Circulating Immunoregulatory Monocytes. PLoS ONE 7(8): e43182.)

Application Data (Published customer image: Renal inflammatory changes in Ntn1+/- mice following ischemia. Ntn1+/- mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A -D) Neutrophil staining. Arrows indicate neutrophils (magnification 400x). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO). (F) TNF-a and (G) interleukin-6 (IL-6) and (H) interleukin-10 (IL-10) were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to beta-actin and are expressed as fold change compared to sham-operated animals without ischemia (-I). Data are representative of four to six independent experiments for each experimental condition (mean +/- SD).From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. (2011) Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.)

Application Data (Published customer image: 166 mM H2O2 increased neutrophil infiltration in day 1 and 6 wounds. Fluorescence intensity of the neodermis was quantified using ImageJ. The area quantified is outlined with the dashed line. Results shown are mean +/- S.E.M, n = 6-7. A representative section from each treatment is shown. ES - Eschar; HE - Hyper-proliferating epidermis; ND - neodermis.*p)

Application Data (Published customer image: Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.From: Tian Y, Linden J, French BA, Yang Z (2014) Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9(12): e114375.)

Application Data (Published customer image: Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 um in length. All micrographs were taken using 200x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

Application Data (Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

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Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12195)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$480/0.1 mg $915/2x0.1 mg $1,325/3x0.1 mg $1,705/4x0.1 mg $1,865/5x0.1 mg

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Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in rat prostate by immunohistochemistry on paraffin embedded sectionsImage caption:Immunohistochemical staining of aromatase in prostate of control and BPA-treated rats at doses of 25, 50, 300, or 600 ug/Kg/d for 4 days.From: Castro B, S¡nchez P, Torres JM, Preda O, del Moral RG, Ortega E. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One. 2013;8(2):e55905.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:P450 arom immunoreactivity in testicular region adjacent to seminoma and in controls A: Intense aromatase immunostaining in IGCN cells. B: Placental-like alkaline phosphatase staining of IGCN basal cells C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc) D: Intense immunostaining of luteal cells (Luc) in ovarian tissue Scale bars: A-B, 8 um; C,12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 use for the detection of aromatase in KGN cells by western blottingImage caption:Effect of H89 on aromatase and BRCA1 expression in KGN cells. A, The human ovarian granulosa cell line KGN were treated with control (DMSO), 20 mM H89, 50 mM PD98059, 200 nM Wortmannin, 100 nM Rapamycin 100 nM, and cells were harvested 24 hours after treatment. Immunoblotting was performed using specific antibodies against aromatase, BRCA1 and a-tubulin; B, Aromatase and BRCA1 mRNA levels were measured by real-time RT-PCR. Gapdh was used for normalizing the real time PCR results. The data are expressed as a fold (Aromatase mRNA), or a percentage (BRCA1 mRNA) relative to the DMSO-treated control.From: Ghosh S, Lu Y, Hu Y. A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression. Int J Biomed Sci. 2008 Dec 15;4(4):260-265.)

Application Data (Western blot analysis of human placenta extract probed with Mouse anti Human Cytochrome P450 Aromatase followed by F(ab')2 Rabbit anti Mouse IgG:HRP)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human lung tumors by immunohistochemistry on formalin fixed paraffin embedded tissue.Image caption:Representative immunohistochemistry of lung tumors for ERa, ERbeta, aromatase, EGFR and PR.From: Stabile LP, Dacic S, Land SR, Lenzner DE, Dhir R, Acquafondata M, Landreneau RJ, Grandis JR, Siegfried JM. Combined analysis of estrogen receptor beta-1 and progesterone receptor expression identifies lung cancer patients with poor outcome. Clin Cancer Res. 2011 Jan 1;17(1):154-64.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in porcine spermatozoa by immunofluorescenceImage caption:Immunofluorescence labelling of androgen and estrogen receptors in pig spermatozoa: A) AR red fluorescence in sperm proximal mid-piece. B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail. C) ERa red fluorescence in the sperm mid-piece, together with a faint labelling in the tail. D) ERbeta green intense light in the sperm acrosomal region. Inserts: immunonegative absorption controls. Scale bars: 5 um.From: Rago V, Aquila S, Panza R, Carpino A. Cytochrome P450arom, androgen and estrogen receptors in pig sperm. Reprod Biol Endocrinol. 2007 Jun 6;5:23.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in sheep tissues by immunohistochemistry on paraffin sectionsImage caption:a and b) Low- (a) and higher- (b) power sections of the fetal end of a placentome in G1 stained with the prolactin antiserum and showing the intensely stained binucleate cells scattered along the trophoblast, but with a tendency to be more densely accumulated towards the fetal end (scale bars a=200?um; b=150?um). (c) High-power section of the trophoblast -endometrium interface in a placentome in G2 stained with the prolactin antiserum. Note the patchy staining of the thin, elongated endometrial epithelial cells (arrowed) in close contact with the intensely stained binucleate trophoblast cells (scale bar=40?um). (d) Low-power section of the intercotyledonary allantochorion stained with the prolactin antiserum and showing the relatively dense accumulation of binucleate trophoblast cells (arrowed) which remain unstained in this region of the placenta (scale bar=100?um). (e) High-power section at the edge of a placentome of G1 showing positive staining of the uninucleate trophoblast by the 3beta-HSD antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um). (f) Section of the distended endometrial glands at the lateral border of a placentome in G2 stained with 3beta-HSD. The basal portions of the endometrial cells lining the glands stain strongly as does the secretory material adhered to the luminal surface of the cells and the accumulated coagulum, possibly as a result of ˜stickiness' of the material (scale bar=150?um). (g) High-power section of the intercotyledonary region of the allantochorion in G1 showing positive staining of the cytoplasm of the uninucleate, but not binucleate trophoblast by the 17,20 lyase antiserum (scale bar=40?um). (h) Section at the fetal end of a placentome from G2 remaining unstained by the aromatase antiserum (scale bar=150?um). (i) Section of near-term sheep placenta showing strong, positive staining of the uninucleate, but not binucleate, trophoblast by the aromatase antiserum (scale bar=150?um). (j) High-power section at the fetal end of a placentome in G1 showing strong positive staining of the uninucleate trophoblast by the PR antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um).From: Wilsher S, Stansfield F, Greenwood RE, Trethowan PD, Anderson RA, Wooding FB, Allen WR. Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis). Reproduction. 2013 May 21;145(6):541-54.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human synovial tissue by immunohistochemistry on cryosectionsImage caption:Aromatase expression in synovial tissue and endogenous steroid hormone release from superfused synovium. (a) Immunohistochemistry of aromatase in one OA and one RA patient. Using the respective control antibody revealed no staining of positive cells (not shown). Magnification: 400x. (b) Density of aromatase-positive cells in OA (open bars, n = 20) and RA patients (hatched bars, n = 16). (c) Spontaneously released E2, E3, and free testosterone from standardized superfused pieces of synovial tissue of OA (open bars, n = 20) and RA patients (hatched bars, n = 18). (b,c) Values are given as box blots with the 5th, 25th, 50th (median), 75th, and 95th percentile when applicable. OA, osteoarthritis; RA, rheumatoid arthritis. Other abbreviations are as given in the legend to Fig. 1.From: Schmidt M, Weidler C, Naumann H, Anders S, Sch¶lmerich J, Straub RH. Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens. Arthritis Res Ther. 2005;7(5):R938-48.)

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CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

Full Name

MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE

Gene Names

CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$1,130/1 mL $2,175/2x1 mL $3,165/3x1 mL $4,095/4x1 mL $4,755/5x1 mL

Structure

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Ochratoxin A, Monoclonal Antibody (Cat# AAA13532)

Full Name

Ochratoxin A (Mouse monoclonal)

Applications

ELISA

Purity

>95% by HPLC & SDS-PAGE
Protein A or G purified and supplied in 0.01 M PBS (pH7.4)

Pricing

$855/1 mg $1,640/2x1 mg $2,380/3x1 mg $3,075/4x1 mg $3,390/5x1 mg

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Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: Biotin)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: RPE)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: Low Endotoxin)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: FITC)

Application Data (Staining of mouse spleen cells with Rat anti Mouse Beta CD102 : Alexa Fluor 488)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD102 : Alexa Fluor 647)

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CD102, Monoclonal Antibody (Cat# AAA11904)

Full Name

RAT ANTI MOUSE CD102:Low Endotoxin

Gene Names

Icam2; CD102; Ly-60; Icam-2

Applications

Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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FCM (Flow Cytometry) (Fig-4: Intracellular flow analysis of TLR7 in Raji cells using 0.5 ug/10^6 cells of TLR7 antibody (Clone: ABM2C27). Green represents isotype control; red represents anti-TLR7 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

IHC (Immunohistochemistry) (Fig-3:Immunohistochemical analysis of TLR7 in Renal Cell Carcinoma using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)

FCM (Flow Cytometry) (Fig-2: Intracellular flow analysis of TLR7 in PBMC (Lymphocyte) using 0.5 ug/10^6cells of TLR7 antibody (Clone: ABM2C27). Green represents isotype control; red represents anti-TLR7 antibody. Goat anti-mouse PE conjugate was used as secondary.)

IHC (Immunohistochemistry) (Fig-1 : Immunohistochemical analysis of TLR7 in human Kidney tissue using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)

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TLR7, Monoclonal Antibody (Cat# AAA14863)

Full Name

TLR7 Monoclonal Antibody

Gene Names

TLR7; TLR7-like

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Purity

Purified; Protein G Chromatography

Pricing

$240/0.025 mg $420/0.1 mg $795/2x0.1 mg $1,145/3x0.1 mg $1,475/4x0.1 mg $1,635/5x0.1 mg

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WB (Western Blot) (Western Blot; Samples: Lane1: human Lung lysate; Lane2: Rat CerebuM lysate; Lane3: Mouse CerebuM lysate;Primary Ab: 2ug/ml Mouse Anti-human NSE AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot; Samples: Lane1: Hela cell lysate; Lane2: Mouse CerebuM lysate; Primary Ab: 2ug/ml Mouse Anti-human NSE Antibody Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Liver TissuePrimary Ab: 30ug/ml Mouse Anti-human NSE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: human Glioma TissuePrimary Ab: 30ug/ml Mouse Anti-human NSE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human CerebuM TissuePrimary Ab: 30ug/ml Mouse Anti-human NSE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-human NSE AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Pancreatic cancer Tissue; Primary Ab: 30ug/ml Mouse Anti-human NSE AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human CerebuM Tissue; Primary Ab: 30ug/ml Mouse Anti-human NSE AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human Glioma Tissue; Primary Ab: 30ug/ml Mouse Anti-human NSE Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Enolase, Neuron Specific (NSE), Monoclonal Antibody (Cat# AAA21249)

Full Name

Monoclonal Antibody to Enolase, Neuron Specific (NSE)

Gene Names

ENO2; NSE

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,440/5 mL $2,780/2x5 mL $4,050/3x5 mL $5,250/4x5 mL $6,415/5x5 mL

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WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1L, Monoclonal Antibody (Cat# AAA24833)

Full Name

HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) (Biotin)

Gene Names

HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HeLa cells were subjected to SDS PAGE followed by western blot with AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:4000)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:200)

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HLA class I (HLA-A), Monoclonal Antibody (Cat# AAA27489)

Full Name

HLA class I (HLA-A) Mouse Monoclonal

Gene Names

HLA-A; HLAA; Aw-33; Aw-74; HLA-A11; HLA-A33; HLA-DQB1; HLA-DRB1

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

>95% as determined by SDS-PAGE
Protein A+G purification

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,550/5x0.1 mg

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)

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VPS35, Monoclonal Antibody (Cat# AAA27705)

Full Name

VPS35 Antibody, Clone 11H10: FITC

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

$665/0.1 mg $1,265/2x0.1 mg $1,845/3x0.1 mg $2,385/4x0.1 mg $2,615/5x0.1 mg

Application Data (TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity was Neutralized by Human TNFR1 Antibody. Recombinant Human TNFR1/TNFRSF1A inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.2 ng/mL) activity elicited by Recombinant Human TNFR1/TNFRSF1A (0.3 ug/mL) is neutralized by increasing concentrations of Human TNFR1/TNFRSF1A Monoclonal Antibody. The IC50 is typically 0.5-1.5 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)

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TNFR1/CD120a/TNFRSF1A, Monoclonal Antibody (Cat# AAA27740)

Full Name

TNFR1/CD120a/TNFRSF1A Neutralizing Antibody

Gene Names

TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2

Applications

Neutralization

Pricing

$385/0.2 mg $555/0.5 mg $1,045/2x0.5 mg $1,510/3x0.5 mg $1,955/4x0.5 mg $2,180/5x0.5 mg

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human colon tissue using FSP1/S100A4 Rabbit mAb (AAA28488, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using FSP1/S100A4 Rabbit mAb (AAA28488) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using FSP1/S100A4 Rabbit mAb (AAA28488) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

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FSP1/S100A4, Monoclonal Antibody (Cat# AAA28488)

Full Name

FSP1/S100A4 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

IF (Immunofluorescence) (Fig.2. Immunofluorescence analysis of human appendix tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

WB (Western Blot) (Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.)

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beta-Tubulin, Monoclonal Antibody (Cat# AAA31540)

Full Name

Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)

Gene Names

TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A

Reactivity

Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Pricing

$170/0.05 mL $210/0.2 mL $390/2x0.2 mL $490/5x0.2 mL $555/3x0.2 mL $705/4x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil.)

IF (Immunofluorescence) (Immunofluorescent analysis using the Antibody at 1:50 dilution.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature)

WB (Western Blot) (All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.)

FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with CCR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. Counter stained with hematoxylin.)

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CCR2, Monoclonal Antibody (Cat# AAA30174)

Full Name

CCR2 Antibody

Gene Names

CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 8A3 depletes VPS35 from the A549 cell extract..)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

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VPS35, Monoclonal Antibody (Cat# AAA27685)

Full Name

VPS35 Antibody, Clone 8A3: ATTO 594

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

$680/0.1 mg $1,300/2x0.1 mg $1,890/3x0.1 mg $2,440/4x0.1 mg $2,665/5x0.1 mg

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Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD195: Azide Free)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD195:RPE)

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CD195, Monoclonal Antibody (Cat# AAA11952)

Full Name

RAT ANTI HUMAN CD195

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Applications

Flow Cytometry

Pricing

$600/0.2 mg $1,140/2x0.2 mg $1,650/3x0.2 mg $2,135/4x0.2 mg $2,385/5x0.2 mg

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Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged Rho GTPase-activating protein 28 protein by western blotting and immunofluorescenceImage caption:Arhgap28-V5 inhibits RhoA activation and stress fiber formation in SaOS-2 cells. SaOS-2 cells were transfected with empty vector or Arhgap28-V5. A. The expression of Arhgap28-V5 was confirmed by western blotting using an antibody to V5. B. Effect of Arhgap28-V5 expression on the basal activity of RhoA (n = 5), Rac1 (n = 3) and Cdc42 (n = 3). Bars show SEM. * indicates significant difference found, p)

Application Data (Published customer image: Mouse anti V5 Tag antibody, clone SV5-Pk1used for immunoprecipitation of V5 tagged RNF11 proteinImage caption:Dynamic associations of RNF11 with both A20 and Itch in primary neurons. N2A cells transduced with V5-RNF11 lentivirus (N2A V5-RNF11) and transfected with DYKDDDDK-A20 were harvested for immunoprecipitation (IP) with V5 antibody (A) or harvested for IP with DYKDDDDK antibody (B). Proteins were resolved by SDS-PAGE and immunoblotted with anti-A20 and RNF11. In parallel, pull-down assays with V5 antibody (C) or with Itch antibody (D) from N2A V5-RNF11 cell lysates were resolved by SDS-PAGE. Immunoprecipitates and lysates were immunoblotted with anti-Itch and RNF11. (E) and (H) Murine primary cortical neurons were stimulated with 10?ng/ml TNF-a for 0 or 30 minutes and harvested for IP with RNF11 antibody. Control IP experiments were performed with antibody omitted. Proteins were resolved by SDS-PAGE and immunoblotted with anti-A20, Itch, RNF11 and actin. (F), (G), (I) and (J) ImageJ software was used to quantify the densitometry of the immunoprecipitated bands relative to the 0-minutes time point. Each input sample's immunoreactivity was used as a loading control. All IPs are representative of at least three independent experiments. *P?)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged podocin protein by western blottingImage caption:The podocin short isoform is N-glycosylated. A) PNGase-F treatment removes the double band from the short isoform in DRM fraction 7. Lysates from Figure 3 were subjected to treatment with PNGase-F and immunoblotted and detected with anti-V5 antibody. B) N to S mutation of the N-glycosylation consensus motif completely abrogates the formation of a double band. The asparagine at position 287 corresponds to amino acid 355 in the full-length protein. HEK293T cells were transfected with V5-tagged Podocin (short isoform or short isoform N287S, respectively) and lysates were immunoblotted and detected with anti-V5 antibody.From: V¶lker LA, Schurek EM, Rinschen MM, Tax J, Schutte BA, Lamkemeyer T, Ungrue D, Schermer B, Benzing T, H¶hne M. Characterization of a short isoform of the kidney protein podocin in human kidney. BMC Nephrol. 2013 May 6;14:102.)

Application Data (V5 tagged protein detected with Mouse anti V5-Tag)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged podocin protein by western blottingImage caption:The podocin short isoform interacts with known podocyte proteins. CD2AP, TRPC6, neprin and NEPH1 co-precipitate with both podocin isoforms (FL, full length (canoncical isoform); SI, short isoform). DYKDDDDK- and V5-tagged proteins were expressed in HEK293T cells and precipitated with anti-DYKDDDDK antibody as indicated. Western blot analysis was performed with a V5 specific antibody. Expression levels of DYKDDDDK.podocin constructs in the lysates are shown below.From: V¶lker LA, Schurek EM, Rinschen MM, Tax J, Schutte BA, Lamkemeyer T, Ungrue D, Schermer B, Benzing T, H¶hne M. Characterization of a short isoform of the kidney protein podocin in human kidney. BMC Nephrol. 2013 May 6;14:102.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not regulated by Cullin E3 Ubiquitin ligases. (A,B) Untransfected (A) or REDD1-V5 pcDNA3 (0.3 ug) transfected (B) HEK293 cells stably expressing tetracycline inducible dnUbc12-HA were induced with 1 ug/ml tetracycline for 24 hours (A,B) or treated with 3 uM MLN4924 (A) or 20 uM MG-132 (A,B) for 8 hours followed by cell lysis. (C,D) Untransfected HEK293 (C) or HEK293 transfected with REDD1-V5 pcDNA3 (0.3 ug) (D) were pre-treated with 3 uM MLN4924 followed by cycloheximide (40 uM) treatment and cell lysis at the indicated time points. (E) HEK293 cells stably expressing tetracycline inducible dnUbc12-HA were induced with 1 ug/ml tetracycline for 24 hours followed by cycloheximide (40 uM) treatment and cell lysis at the indicated time points.From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged HIVA peptide by western blottingImage caption:Construction of the BCG.HIVACAT vaccine strain. (A) A synthetic GC-rich HIVA gene was fused to the region encoding the 19-kDa lipoprotein signal sequence and inserted into the episomal pJH222 E Coli-mycobacterium shuttle plasmid. This plasmid contains kanamycin resistance (aph) and complementing lysA genes and an E Coli origin of replication (oriE). In addition, pJH222 contained the mycobacterial origin of replication (oriM). The BALB/c mouse T-cell and MAb Pk epitopes used in this work are depicted. P a-Ag, M. tuberculosis a-antigen promoter; PHSP60, heat shock protein 60 gene promoter. The aph gene was removed by SpeI digestion and the lacO sequence was inserted and transformed into E Coli DH1lacdapD strain. (B) Immunodot of BCG.HIVACAT lysates. Dot 1: BCG wild type (negative control); Dot 2, 3, 4 and 5: clone 3, clone 7, clone 9 and clone 10 of BCG.HIVACAT; Dot 6: BCG.HIVA222 (positive control). HIVA peptide was detected using the anti-Pk MAb followed by horseradish peroxidase-Goat-anti-Mouse and enhanced chemiluminescence (ECL) detection. (C) In vivo plasmid stability of BCG.HIVACAT harboring pJH222.HIVACAT. Mice were injected s.c. with 105 cfu of BCG.HIVACAT and boosted i.m. with 106 pfu of MVA.HIVA, spleens were homogenized 20 weeks after BCG inoculation and the recovered rBCG colonies were tested for the presence of the HIVA DNA coding sequence by PCR. Lanes 1 to 6: Six rBCG colonies were recovered in the non-lysine supplemented plate; lane 7: Molecular weight marker; lane 8: Plasmid DNA positive control; lane 9: Distilled water (negative control).From: Saubi N, Mbewe-Mvula A, Gea-Mallorqui E, Rosario M, Gatell JM, et al. (2012) Pre-Clinical Development of BCG.HIVACAT, an Antibiotic-Free Selection Strain, for HIV-TB Pediatric Vaccine Vectored by Lysine Auxotroph of BCG. PLoS ONE 7(8): e42559.)

Application Data (Published customer image: Mouse anti V5 Tag antibody, clone SV5-Pk1used for immunoprecipitation of V5 tagged RNF11 proteinImage caption:Myristoylation mutant of RNF11 is unable to associate with Itch. SH-SY5Y shRNA-RNF11 cells were transfected with shRNA-resistant RNF11 constructs or vector. Coimmunoprecipitation experiments using V5 antibody were performed 24 hours after transfection. Immunoprecipitates and lysates were resolved by SDS-PAGE and immunoblotted with anti-A20, Itch, RNF11 or actin. Blots are representative of three independent experiments. From: Pranski EL, Dalal NV, Herskowitz JH, Orr AL, Roesch LA, Fritz JJ, Heilman C, Lah JJ, Levey AI, Betarbet RS. Neuronal RING finger protein 11 (RNF11) regulates canonical NF-?B signaling. J Neuroinflammation. 2012 Apr 16;9:67. doi: 10.1186/1742-2094-9-67.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

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V5-TAG, Monoclonal Antibody (Cat# AAA12081)

Full Name

MOUSE ANTI V5-TAG:HRP

Applications

Western Blot

Pricing

$620/0.1 mg $1,180/2x0.1 mg $1,710/3x0.1 mg $2,210/4x0.1 mg $2,480/5x0.1 mg

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Distribution of the nerve net in two-day-old planula detected using YL1/2 antibody. (A) Superficial view (optical plane on the basal part of the ectodermal epithelium). (B) Deeper view of the same specimen (optical plane crossing the larval endoderm and cavity). (C) Higher magnification view of the YL1/2 staining (in red) with Dapi counter-staining (in blue) showing the distribution and aspect of nerve cell bodies (white arrowheads) and neurites. Oral pole is on the top for all pictures. Scale bars: A-B, 50 um; C, 10 um.From: Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica Muriel Jager, Eric Qu©innec, Herv© Le Guyader and Micha«l Manuel. EvoDevo 2011, 2:12.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

Application Data (HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP)

Application Data (Western blot analysis of C6 rat glioma whole cell lysate probed with Rat anti tubulin alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

Application Data (Published customer image: Direct detection of the M42 polypeptide. (A) Validation of anti-M42 serum. Lysates from cells transfected with the indicated GFP polypeptides were analysed by SDS-PAGE and western blotting as labeled. (B) Detection of M42 from virus-infected cells. Lysates from cells infected with the indicated viruses at 10 h p.i. were analysed by SDS-PAGE and western blotting as labeled. The same membrane was probed with mouse anti-M2 14C2 and rabbit anti-M42 using different colour secondary antisera; individual grey scale and colour merged images are shown.From: Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, et al. (2012) Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain. PLoS Pathog 8(11): e1002998.)

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TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

$555/0.1 mg $1,045/2x0.1 mg $1,510/3x0.1 mg $1,955/4x0.1 mg $2,165/5x0.1 mg

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Distribution of the nerve net in two-day-old planula detected using YL1/2 antibody. (A) Superficial view (optical plane on the basal part of the ectodermal epithelium). (B) Deeper view of the same specimen (optical plane crossing the larval endoderm and cavity). (C) Higher magnification view of the YL1/2 staining (in red) with Dapi counter-staining (in blue) showing the distribution and aspect of nerve cell bodies (white arrowheads) and neurites. Oral pole is on the top for all pictures. Scale bars: A-B, 50 um; C, 10 um.From: Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica Muriel Jager, Eric Qu©innec, Herv© Le Guyader and Micha«l Manuel. EvoDevo 2011, 2:12.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

Application Data (HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP)

Application Data (Western blot analysis of C6 rat glioma whole cell lysate probed with Rat anti tubulin alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

Application Data (Published customer image: Direct detection of the M42 polypeptide. (A) Validation of anti-M42 serum. Lysates from cells transfected with the indicated GFP polypeptides were analysed by SDS-PAGE and western blotting as labeled. (B) Detection of M42 from virus-infected cells. Lysates from cells infected with the indicated viruses at 10 h p.i. were analysed by SDS-PAGE and western blotting as labeled. The same membrane was probed with mouse anti-M2 14C2 and rabbit anti-M42 using different colour secondary antisera; individual grey scale and colour merged images are shown.From: Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, et al. (2012) Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain. PLoS Pathog 8(11): e1002998.)

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TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

Pricing

$820/0.5 mg $1,565/2x0.5 mg $2,270/3x0.5 mg $2,940/4x0.5 mg $3,350/5x0.5 mg

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power)

Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody (AAA12011. A in red) and Mouse anti Human CD21 . C is the merged image. Low power)

Application Data (Staining of MOLT 4 cells with Mouse anti Human CD1a)

Application Data (Staining of MOLT 4 cells with Mouse anti Human CD1a:RPE)

Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody (AAA12011. A in red) and Mouse anti Human CD21 . C is the merged image. High power)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. High power)

Application Data (Staining of MOLT 4 cells with Mouse anti Human CD1a:FITC)

Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody (AAA12011. A in red) and Mouse anti Human CD21 . C is the merged image. Medium power)

Application Data (Published customer image: CLR expression on various DC subsets present in the endometrium. (a) Langerin positive cells were mainly detected within the luminal and glandular epithelium. Positive co-localization (yellow) was detected with Langerin+ cells (green) and (b) CD1a (red) and (c) CD11c (red). (d) Double-positive cells (yellow) expressing Langerin+ (green) and CD4+ (red) cells were also detected within CD4+ lymphoid aggregates. (e) DC-SIGN and (f) MR were detected in the lamina superficialis, just beneath the luminal epithelium (I: minimum distance of cell body to endometrial lumen: 49 um and 40 um, respectively). Both (g) DC-SIGN (green) and (h) MR(green) were co-expressed (yellow) on CD68+ cells (red) as well as (i and j, respectively) CD11c+ cells (red). Neither (k) DC-SIGN (red) nor (l) MR (red) were expressed on CD1a+ (green) cells. Scale bars: a: 500 um; b -d: 25 um; e -l: 100 um.From: Kaldensj¶ T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011) Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection. PLoS ONE 6(6): e21344.)

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CD1a, Monoclonal Antibody (Cat# AAA12011)

Full Name

MOUSE ANTI HUMAN CD1a

Gene Names

CD1A; R4; T6; CD1; FCB6; HTA1

Reactivity

Cynomolgus monkey, Dog

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$600/0.2 mg $1,140/2x0.2 mg $1,650/3x0.2 mg $2,135/4x0.2 mg $2,385/5x0.2 mg

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Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:NKp46high NK cells in spleen showed the highest cytolytic capacity. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8?+NKp46-: blue, CD8?+NKp46+: green, CD8?dim/-NKp46high: red) isolated from spleen was analysed after receptor-mediated degranulation. Cells were stimulated and gated for flow cytometric analysis as outlined in Figure 4. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p )

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Analysis of degranulation capacity in NKp46-defined NK-cell subsets in blood. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8?+NKp46-: blue, CD8?+NKp46+: green) isolated from blood was analysed after receptor triggering. Cells were stimulated with rhIL-2 and rpIL-15 overnight. Triggering of NK-receptors was performed by using monoclonal antibodies against NKp46, CD16 or a combination of both. Irrelevant isotype-matched antibody served as negative control. NK-cell receptor mediated degranulation was assessed by measuring the expression of CD107a on the cell surface by four-colour flow cytometry after one hour incubation. CD107a expression was measured on CD3- lymphocytes (not shown), followed by gating on the respective NKp46-defined NK subsets. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p )

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Splenic NKp46high NK cells produced the highest levels of IFN-?. (A + B) Intracellular cytokine staining for IFN-? production in NKp46-defined NK cells within PBMC (upper graphs) and splenocytes (lower graphs) by four-colour FCM after 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18 or medium. CD3- lymphocytes were gated (not shown) and NKp46/CD8? defined total NK cells were further subgated for IFN-? production. IFN-? producing NK cells were gated according to NKp46 expression levels (CD8?+NKp46-: blue, CD8?+NKp46+: green, CD8?dim/-NKp46high: red). (A) Numbers indicate the percentage of IFN-?+ NK cells within respective gates. Results are representative for experiments with four different animals. (B) Percentage of IFN-?+ cells (left graph) and mean fluorescence intensity of IFN-?+ cells (right graph) within the different NK-cell subsets in blood and spleen of four animals are shown. (C) Supernatants of cytokine stimulated FACS-sorted CD3-CD8?+NKp46- and CD3-CD8?+NKp46+ NK cells from blood and CD3-CD8?+NKp46-, CD3-CD8?+NKp46+ and CD3-CD8?dim/-NKp46high NK cells from spleen were tested for IFN-? production in ELISA following 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18. Data on the left are from one representative animal and displayed as the mean of duplicates ± SD. IFN-? production in experiments with four animals analysed is shown on the right. Mean values are represented by a black bar. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p )

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Varying expression of NKp46, CD8?, CD16 and CD27 on NK-cell subsets in blood and spleen. (A) Following five-colour staining, PBMC and splenocytes were gated on CD3- cells and further subgated according to their NKp46/CD8? expression pattern in NKp46- and NKp46+ NK cells in blood (upper graph) and NKp46-, NKp46+ and NKp46high NK cells in spleen (lower graph). (B) 14 healthy 6–7 month old pigs were investigated for NKp46 and CD8? expression levels in the NKp46-defined NK-cell subsets. Box-plots show the mean fluorescence intensity of the two markers. (C) NK-cell subsets defined in (A) were further analysed for their expression of the surface markers CD16 and CD27. Histograms show the expression of the two markers within the respective NKp46-defined subsets (CD8?+NKp46-: blue histograms, CD8?+NKp46+: green histograms, CD8?dim/-NKp46high: red histograms) in blood (upper graphs) and spleen (lower graphs) according to the corresponding isotype control (grey histograms with dotted lines). Box-plots show the mean fluorescence intensity of CD16 and CD27 of the NKp46-defined NK-cell subsets in blood and spleen of 14 healthy 6–7 month old pigs. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p )

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Varying expression of NKp46, CD8?, CD16 and CD27 on NK-cell subsets in blood and spleen. (A) Following five-colour staining, PBMC and splenocytes were gated on CD3- cells and further subgated according to their NKp46/CD8? expression pattern in NKp46- and NKp46+ NK cells in blood (upper graph) and NKp46-, NKp46+ and NKp46high NK cells in spleen (lower graph). (B) 14 healthy 6–7 month old pigs were investigated for NKp46 and CD8? expression levels in the NKp46-defined NK-cell subsets. Box-plots show the mean fluorescence intensity of the two markers. (C) NK-cell subsets defined in (A) were further analysed for their expression of the surface markers CD16 and CD27. Histograms show the expression of the two markers within the respective NKp46-defined subsets (CD8?+NKp46-: blue histograms, CD8?+NKp46+: green histograms, CD8?dim/-NKp46high: red histograms) in blood (upper graphs) and spleen (lower graphs) according to the corresponding isotype control (grey histrograms with dotted lines). Box-plots show the mean fluorescence intensity of CD16 and CD27 of the NKp46-defined NK-cell subsets in blood and spleen of 14 healthy 6–7 month old pigs. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p )

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescenceImage caption:Counting of NKp46+ cells. Numbers of NKp46+ cells per area were counted in lung tissue sections from control animals and influenza A virus infected animals as described in Material and Methods. Representative picture of area with virus from infected animal. Immunofluorescence staining, 200x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014)Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.)

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:Staining for apoptosis in the lungs. Lung tissue sections from animals infected with influenza A virus (n = 12) were stained with immunofluorescence markers against (A) NKp46(green), (B) influenza A NP(red) and (C) the apoptosis marker caspase-3(blue). (D) Overlay displaying simultaneously influenza A virus NP+ and caspase-3+ cells as purple (arrows). Representative of virus infected bronchiole at day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014)Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.)

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:NKp46+ cells in the lungs of influenza virus infected pigs. Lung tissue sections from pigs infected with influenza A virus and control pigs were stained with immunofluorescence markers for cytokeratin (blue), NKp46 (green) and influenza A virus NP (red). NKp46+ cells were counted in areas were influenza A virus NP was (A) detected and (B) not detected. Representative pictures taken from the same animal on day 1 pi are shown. Arrows point at NKp46+ cells. Immunofluorescence staining, 200x. (C) Plot shows number of NKp46+ cells per 0,1 mm2 in sections (n = 24 per animal) from control animals (n = 6) and in areas with and without virus in infected animals (n = 4 per day) calculated as described in Material and Methods. Groups with different letters differ significantly (p?0.05). (D) NKp46+ cells in the lumen of a bronchus (BL). Arrows point at the epithelial lining. Representative picture of luminal exudate, taken from an infected animal on day 2 pi. Insert shows NKp46+ and influenza A virus NP+ cell in the lung tissue of an infected animal on day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014)Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.)

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption:Percentages of NK cells and expression of NKp46 and CD25 in lung tissue. Mononuclear cells were isolated from lung tissue of pigs infected with influenza A virus (n = 12) and control animals (n = 6) during the first 3 days pi and analysed by flow cytometry. (A) Live CD3? lymphocytes were gated as described in Fig 2. NK cells were gated according to CD8? and NKp46 expression and defined as NKp46?, NKp46int or NKp46high cells. Plot shown is from a representative control animal. (B) Proportions of NKp46? (green), NKp46int (blue) and NKp46high (purple) NK cells in individual animals, shown as percentages of gated cells in lymphocytes. (C) Percentages of NKp46? (left), NKp46int (middle) and NKp46high (right) NK cells among lymphocytes. (D) Median fluorescence intensity (MFI) in the NKp46? gate (left), the NKp46+ gate (middle) and in the NKp46high gate (right) are shown. (E) CD25+ cells were gated in the NKp46? and NKp46int NK cells (left) and in the NKp46high NK cells (right). Plots shown are from a representative control animal. (F) The percentages of CD25+ cells in each gate were calculated in control animals (green), infected animals from day 1 (purple), day 2 (blue) and day 3 (pink) pi. *p?0,05, **p?0.01.)

Application Data (Published investigator image:Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption:NK cell numbers in the blood of influenza infected pigs. Blood was taken from influenza A virus infected (n = 12) and control pigs (n = 9) on day 0?3 pi in a second experiment. (A) Isolated PBMCs were analysed by flow cytometry and live CD3? lymphocytes were gated as NKp46? or NKp46+ NK cells according to CD8? and NKp46 expression. Plots are taken from a representative control animal. Absolute numbers of (B) NKp46+ NK cells in PBMC were analysed by flow cytometry in control (left) and infected animals (right). (C) Results for the NKp46+ cells in the two groups were compared on each sampling day. (D) NKp46? NK cells in PBMC in control (left) and infected animals (right). (E) NKp46? NK cells were compared for the two groups. Each line in (B) and (D) represents one animal. **p?0.01.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014)Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.)

Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335 detected with Goat anti Mouse IgG (H/L):FITC , and Mouse anti Pig wCD8a:RPE)

Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:Alexa Fluor®488 and Mouse anti Pig wCD8a:RPE)

Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:APC and Mouse anti Pig wCD8a:RPE)

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CD335, Monoclonal Antibody (Cat# AAA12295)

Full Name

MOUSE ANTI PIG CD335:RPE

Reactivity

Pig

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

$795/100 Tests $1,520/2x100 Tests $2,210/3x100 Tests $2,860/4x100 Tests $3,250/5x100 Tests

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IHC (Immunohistochemistry) (Immunohistochemical analysis of HMGB1  in normal human spleen tissue using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of HMGB1  in squamous cell carcinoma of esophagus using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of HMGB1  in normal human prostate tissue using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of HMGB1  in adenocarcinoma of stomach using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

FCM (Flow Cytometry) (Intracellular flow analysis of HMGB1 in PBMC (Lymphocyte) using 0.5 µg/10^6 cells of HMGB1 antibody (Clone: ABM24D3). Green represents isotype control; red represents anti-HMGB1 antibody. Goat anti-mouse PE conjugate was used as secondary.)

FCM (Flow Cytometry) (Intracellular flow analysis of HMGB1 in PBMC (Monocyte) using 0.5 µg/10^6 cells of HMGB1 antibody (Clone: ABM24D3). Green represents isotype control; red represents anti-HMGB1 antibody. Goat anti-mouse PE conjugate was used as secondary.)

WB (Western Blot) (Expression analysis of HMGB1. Anti- HMGB1 antibody (Clone: ABM24D3) was used at 2 µg/ml on HepG2 lysates.)

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HMGB1, Monoclonal Antibody (Cat# AAA14866)

Full Name

HMGB1 Monoclonal Antibody

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human (Predicted Reactivity: Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$240/0.025 mg $420/0.1 mg $795/2x0.1 mg $1,145/3x0.1 mg $1,475/4x0.1 mg $1,635/5x0.1 mg

IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)

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Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)

Full Name

Mast Cell Chymase (BSA & Azide Free)

Gene Names

CMA1; CYH; MCT1; chymase; MGC119890; MGC119891

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified by Protein G affinity chromatography.

Pricing

$1,105/0.1 mL $2,125/2x0.1 mL $3,090/3x0.1 mL $4,000/4x0.1 mL $4,675/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse intestine tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of SK-MEL-28 cells using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of Hep G2 cells using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using CD66a/CEACAM1 Rabbit PolymAb® (AAA28579) at 1:3000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): HeLaExposure time: 30s.)

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CD66a/CEACAM1, Monoclonal Antibody (Cat# AAA28579)

Full Name

CD66a/CEACAM1 Rabbit PolymAb

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

WB (Western Blot) (Western blot analysis of Lipoprotein lipase expression in Human fetal liver lysate (AAA11694).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LPL)

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Lipoprotein lipase, Monoclonal Antibody (Cat# AAA11694)

Full Name

Anti-Lipoprotein lipase Rabbit Monoclonal Antibody

Gene Names

LPL; LIPD; HDLCQ11

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$240/0.03 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,030/5x0.1 mL

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Application Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)

Application Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178:RPE)

Application Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178:Alexa Fluor 488)

Application Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178)

Application Data (Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor 647)

Application Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178)

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CD178, Monoclonal Antibody (Cat# AAA11962)

Full Name

MOUSE ANTI HUMAN CD178

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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Application Data (Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)

Application Data (Staining of frozen rat spleen with Mouse anti Rat CD4)

Application Data (Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. High power)

Application Data (Staining of rat splenocytes with Mouse anti Rat CD4)

Application Data (Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. Low power)

Application Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD4)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)

Application Data (Published customer image: Dynamics of T-regulatory cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 um.From: Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)

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CD4, Monoclonal Antibody (Cat# AAA11994)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$960/1 mg $1,855/2x1 mg $2,690/3x1 mg $3,480/4x1 mg $4,010/5x1 mg

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Application Data (Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)

Application Data (Published customer image: Transduction of AdV-eGFP/AdV-ZFP-VEGF into the spinal cord. (A) Photomicrographs showing a transverse section of rat spinal cord obtained adjacent to the injury site 10 days after spinal cord injury and AdV-eGFP injection. eGFP signal was detected in both the gray matter and white matter. (B) High-power (63X) confocal images show that the AdV-eGFP vector (green) transfected neurons (NeuN), astrocytes (GFAP), oligodendrocytes (CC1) and endothelial cells (RECA-1). Cells have been counter-stained with DAPI (blue) as nuclear marker. (C) Bar graph displays quantification of transduced cell types +/- SEM, as identified by the cell-specific markers NeuN, GFAP, RECA-1 and CC1. (D) Evaluation of AdV-ZFP-VEGF gene transfer. Western blot showed that the NF?B p65 rabbit polyclonal antibody recognizes the p65 activation domain in the AdV-ZFP-VEGF treated animals. The higher molecular weight bands are endogenous NF?Bp65 fragments, which are also recognized by the antibody; however, these bands are present in both the control and treatment groups. The lower band (arrow) corresponds to the AdV-ZFP-VEGF and was only present in the treated animals. Lower panel shows actin expression as a protein control. Scale bar: 1000 um for A; 100 um for B.From: Figley SA, Liu Y, Karadimas SK, Satkunendrarajah K, Fettes P, et al. (2014) Delayed Administration of a Bio-Engineered Zinc-Finger VEGF-A Gene Therapy Is Neuroprotective and Attenuates Allodynia Following Traumatic Spinal Cord Injury. PLoS ONE 9(5): e96137.)

Application Data (Published customer image:Endothelial cell number and capillary length post radiation. (A) Representative images of sections from the corpus callosum immunostained for rat endothelial cell antigen (RECA) at various time points post radiation. RECA expression declines immediately post radiation but is restored and maintained through 15 months. Stereological estimates of the number of capillary segments in the cortex (B) and corpus callosum (C) and of capillary length in both regions (D, E). (*** p)

Application Data (Published customer image: Pyruvate reduces R/M hypoglycemia-induced cortical blood vessel loss. (A) Bright field photomicrographs from coronal sections of cortex demonstrate loss of blood vessels at three days after R/M hypoglycemia by RECA-1 (rat endothelial cell antigen 1) staining. Panels show the progression of blood vessel changes in the parietal (PT Ctx) and perirhinal (PRh Ctx) cortex. After R/M hypoglycemia, blood vessels showed decreased density compared to sham-operated rats. Intraperitoneal injection of pyruvate as an adjuvant to glucose at ten minutes after R/M hypoglycemia reduced blood vessel disappearance. Scale bar = 100 um. (B) Graph represents the % area of RECA-1 immunoreactivity in the parietal and perirhinal cortex. Data are means +/- s.e.m., n=5-6 from each group, *P)

Application Data (Published customer image: AdV-ZFP-VEGF results in increased vessel counts and angiogenesis. (A) Left panel: Illustration of the area of spinal cord areas used for RECA-1 counting (2 grey matter areas, 2 white matter areas). (B) Representative sections taken 2 mm rostral to the epicenter from a AdV-ZFP-VEGF treated and AdV-eGFP control animal respectively immunostained with RECA-1 at 10 days after SCI; scale 100 um. An increased number of vessels were observed in the AdV-ZFP-VEGF treated group. (C) Bar graph illustrating the RECA-1 positive cell counts 10 days after SCI. AdV-ZFP-VEGF administration resulted in a significant increase in vascular counts (2 mm and 4 mm away from the epicenter) as compared with the control group. (D) Representative confocal image from an ADV-ZFP-VEGF treated animal at 5 days post-injury. Image was taken at 2 mm rostral from the epicenter, and shows double-labeled cells. Cells were stained for endothelial cells (RECA-1, green) and proliferation (Ki67, red). Scale bar = 50 um (30 um for magnified panel). (E) Angiogenesis was assessed by quantifying Ki67/RECA-1 co-labeled vessels. Data is presented at the percentage of RECA-1+ vessels that were also Ki67+, with an overall average increase of 10% vascular proliferation observed in the animals receiving AdV-ZFP-VEGF administration. All data are presented as mean +/- SEM, and was analyzed by Two-way ANOVA (Holm-Sidak post-hoc). Angiogenesis data were analyzed by performing an arcsine transformation of the values, prior to Two-way ANOVA and post-hoc testing. *p)

Application Data (Published customer image:Representative patterns of RECA-1 staining in rat hippocampus. (a) Microvessels in control (PBS) injected rat hippocampus (left panel) and microvessels in Abeta1-42-injected hippocampus (right panel). Scale bar is for 70um. (b) Bar graph for the number of microvessels/mm2 (n = 5 each). (c) Bar graph for microvessel length (n = 5 each). *P = 0.05 for Abeta1-42 versus PBS.From: Jantaratnotai N, Ryu JK, Schwab C, McGeer PL, McLarnon JG. Comparison of Vascular Perturbations in an Abeta-Injected Animal Model and in AD Brain. Int J Alzheimers Dis. 2011;2011:918280.)

Application Data (Published customer image:Morphology of microvessels stained with RECA-1 in Abeta 1-42-injected hippocampus. Panels show morphological features including fragments, looping microvessels, and vessels with knob-like and uneven diameters. The scale bar represents 40?um.From: Jantaratnotai N, Ryu JK, Schwab C, McGeer PL, McLarnon JG. Comparison of Vascular Perturbations in an Abeta-Injected Animal Model and in AD Brain. Int J Alzheimers Dis. 2011;2011:918280.)

Application Data (Published customer image: ChABC and GF treatments attenuate the proliferation of microglia/macrophages and promote the generation of new endothelial cells after SCI. (A -D) Representative confocal images of BrdU+/NG2+ macrophages/microglia marked with Iba-1 in the injured spinal cord (arrows). (E -F) Under baseline SCI condition, macrophages/microglia comprised about 25% and 17% of BrdU+/NG2+ cells in rostral and caudal points to the injury center, respectively. After treatment with ChABC and/or GFs, we found a reduction in the number of BrdU+/NG2+/IbA-1+ cells that was statistically significant for ChABC and ChABC+GFs treatment groups relative to the vehicle group. (G -J) Representative confocal images show newly generated endothelial cells marked by Reca-1 and NG2 among BrdU+ cells. Reca-1 positive endothelial cells comprised a subpopulation of proliferating NG2+ cells after SCI (J). (K -L) Quantification of BrdU+/NG2+/Reca-1+ cells showed a significant number of newly generated endothelial cells after treatment with ChABC and/or GFs at both rostral and caudal points to the injury center compared to the vehicle group. *p)

Application Data (Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. (A) Distribution of BrdU(+) endothelial cells. Brain of the unilaterally lesioned rats 6 weeks after infusion of clustered ephrin-A1-Fc were sectioned coronally and stained for BrdU and Rat Endothelial Cell Antigen-1 (RECA-1). Numbers of the BrdU(+) cells and BrdU(+)&RECA-1(+) cells were counted as describe in the Materials and Methods. Total numbers of BrdU(+) cells in 8 animals are shown on the top, and percentages of RECA-1(+) cells among BrdU(+) cells are shown on the bottom. Error bars represent SD. *p)

Application Data (Published customer image:hADSCs led to the appearance of perivascular spaces in between endothelial and astrocytic basement membranes one week after injection. A -D) Confocal images of horizontal sections immunostained with anti-pan-laminin antibody (red) one week after injury. Note that in DMEM animals (A,B) there is no separation between the two membranes whereas in hADSCs -treated animals (C,D) these membranes are separated (arrows in D). E) Confocal images of a horizontal section immunostained with anti-pan-laminin (green) and RECA-1(red). F -F) Confocal imagens of sequential optical sections immunostained with anti-pan-laminin (green) and DAPI (blue) showing the extravasation of cells from the blood vessels. Bars: C, F = 50 um B, D, E = 25 um.From: Menezes K, Nascimento MA, Gon§alves JP, Cruz AS, Lopes DV, et al. (2014) Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. PLoS ONE 9(5): e96020.)

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RECA-1, Monoclonal Antibody (Cat# AAA12018)

Full Name

MOUSE ANTI RAT RECA-1

Applications

Immunohistochemistry, Immunofluorescence

Pricing

$460/0.1 mg $875/2x0.1 mg $1,260/3x0.1 mg $1,635/4x0.1 mg $1,760/5x0.1 mg

WB (Western Blot) (The following image is the result of using mAb 5B8 ascites at 1:5000 dilution to detect Abeta40 and Abeta42 on Western blot. Lane 1: Abeta42, Lane 2: Abeta40.)

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amyloid beta peptide N-terminal, Monoclonal Antibody (Cat# AAA14629)

Full Name

mAb anti-human amyloid beta peptide N-terminus

Reactivity

Human

Applications

Western Blot

Purity

Protein G affinity purified

Pricing

$430/0.1 mg $580/0.5 mg $1,100/2x0.5 mg $1,595/3x0.5 mg $2,055/4x0.5 mg $2,155/5x0.5 mg

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WB (Western Blot) (Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).)

SDS-PAGE (SDS-PAGE Analysis Purified Estrogen Receptor beta1 Mouse Monoclonal Antibody (ERb455). Confirmation of Integrity and Purity of Antibody.)

FCM (Flow Cytometry) (Flow Cytometry of human ER beta on BT474 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Monoclonal Antibody (Erb455).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

FCM (Flow Cytometry) (Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ERb455).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

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ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13814)

Full Name

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.