Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IP (Immunoprecipitation) (Immunoprecipitation of CDK1 from 300 µg extracts of 293T cells was performed using 3 µg of CDK1 Rabbit mAb (AAA28457). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using CDK1 Rabbit mAb (AAA28457) at a dilution of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CDK1 Rabbit mAb (AAA28457) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using CDK1 Rabbit mAb (AAA28457) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CDK1 Rabbit mAb (AAA28457) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of lysates from Mouse spleen, using CDK1 Rabbit mAb (AAA28457) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of various lysates using CDK1 Rabbit mAb (AAA28457) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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CDK1, Monoclonal Antibody (Cat# AAA28457)

Full Name

CDK1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Overlay Peak curve showing HepG2 cells stained with AAA28068 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

FCM (Flow Cytometry) (Overlay Peak curve showing Hela cells stained with AAA28068 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with AAA28068 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of NIH/3T3 cells with AAA28068 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

IHC (Immunohistochemistry) (IHC image of AAA28068 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37?. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG labeled by HRP and visualized using 0.05% DAB.)

IHC (Immunohistochemistry) (IHC image of AAA28068 diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.)

WB (Western Blot) (Western Blot Positive WB detected in: NFE2L2 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: RAW264.7 whole cell lysate Lane 6: K562 whole cell lysateSecondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 68 KDa Observed band size: 68-100 KDa Exposure time: 1min)

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NFE2L2, Monoclonal Antibody (Cat# AAA28068)

Full Name

Mouse anti-Homo sapiens (Human) NFE2L2 Monoclonal antibody

Gene Names

NFE2L2; NRF2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

>95%, Protein G purified

Pricing

$305/0.05 mL $400/0.1 mL $750/2x0.1 mL $1,090/3x0.1 mL $1,400/4x0.1 mL $1,705/5x0.1 mL

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FCM (Flow Cytometry) (Overlay Peak curve showing 293T transfected cells stained with AAA28069 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with MERTK AAA28069 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of 293T transfected cells with MERTK AAA28069 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

WB (Western Blot) (Western BlotPositive WB detected in: 293 whole cell lysate, SY5Y whole cell lysate, K562 whole cell lysate All lanes: MERTK antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 110 kDaObserved band size: 130 KDaExposure time?5min)

WB (Western Blot) (Western BlotPositive WB detected in: 1-4 lanes: Recombinant MERTK protein at 20ng, 10ng, 5ng, 2.5ngAll lanes: MERTK antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 120 KDaObserved band size: 120-150 KDaExposure time?5S)

WB (Western Blot) (Western BlotPositive WB detected in: 1-4 lanes: 293T whole cell lysate transfected with MERTK at 10ug, 5ug, 2.5ug, 1.25ugAll lanes: MERTK antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 150-250 KDaObserved band size: 150-250 KDaExposure time?5S)

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MERTK, Monoclonal Antibody (Cat# AAA28069)

Full Name

Mouse anti-Homo sapiens (Human) MERTK Monoclonal antibody

Gene Names

MERTK; MER; RP38; c-mer

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence, Flow Cytometry

Purity

>95%, Protein A purified

Pricing

$305/0.05 mL $400/0.1 mL $750/2x0.1 mL $1,090/3x0.1 mL $1,400/4x0.1 mL $1,705/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of Raji cell lysates using SHP-1 mouse mAb.)

WB (Western Blot) (Western blot detection of SHP-1 in Human tonsil, Daudi, Jurkat and Raji cell lysates using SHP-1 mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.)

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SHP-1, Monoclonal Antibody (Cat# AAA14102)

Full Name

Anti-SHP-1 Mouse mAb

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Purity

Affinity purified

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)

IF (Immunofluorescence) (Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red dot (Red).)

FCM (Flow Cytometry) (Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (RIV9) (green))

FCM (Flow Cytometry) (Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9).Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red))

SDS-PAGE (SDS-PAGE AnalysisCD3e Mouse Monoclonal Antibody (RIV9).Confirmation of Integrity and Purity of Antibody)

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CD3, Monoclonal Antibody (Cat# AAA13839)

Full Name

CD3 (T-Cell Marker) Mouse Monoclonal Antibody

Gene Names

CD3E; T3E; TCRE; IMD18

Reactivity

Human, Mouse, Rat

Applications

Functional Assay, Flow Cytometry, Immunofluorescence

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA at 1mg/mL)

Application Data

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CD30, Monoclonal Antibody (Cat# AAA14859)

Full Name

anti-human CD30-R-PE

Reactivity

Human

Applications

Flow Cytometry

Pricing

$690/120 Tests $1,325/2x120 Tests $1,915/3x120 Tests $2,475/4x120 Tests $2,830/5x120 Tests

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WB (Western Blot) (Western Blot; Sample: Hela cell lysate Primary Ab: 0.2ug/ml Mouse Anti-Rat CALR Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Stomach Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat CALR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat CALR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Testis Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat CALR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Rat Cardiac Muscle Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat CALR AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: Rat Colon Tissue; Primary Ab: 30ug/ml Mouse Anti-Rat CALR Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Calretiulin (CALR), Monoclonal Antibody (Cat# AAA21254)

Full Name

Monoclonal Antibody to Calretiulin (CALR)

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$255/0.1 mL $320/0.2 mL $520/0.5 mL $625/1 mL $1,650/5 mL $3,190/2x5 mL $4,645/3x5 mL $6,020/4x5 mL $7,330/5x5 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 8A3 depletes VPS35 from the A549 cell extract..)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

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VPS35, Monoclonal Antibody (Cat# AAA27687)

Full Name

VPS35 Antibody, Clone 8A3: FITC

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

$665/0.1 mg $1,265/2x0.1 mg $1,845/3x0.1 mg $2,385/4x0.1 mg $2,615/5x0.1 mg

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)

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VPS35, Monoclonal Antibody (Cat# AAA27700)

Full Name

VPS35 Antibody, Clone 11H10

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

$620/0.1 mg $1,180/2x0.1 mg $1,710/3x0.1 mg $2,210/4x0.1 mg $2,395/5x0.1 mg

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Application Data (10C12 inhibited the tumor growth of COLO205 on nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 70.0% at 5 mpk at D26.)

Application Data (The endocytosis ratio 10C12 by Human CDH17 HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 2 nM.)

FCM (Flow Cytometry) (Human CDH17 HEK293 cells were stained with Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC240=0.06889 ug/mL)

Application Data (Immobilized human CDH17, His Tag at 2 ug/mL can bind Anti-CDH17 / Cadherin-17 Reference Antibody (10C12), EC50=0.00468ug/mL)

Application Data (The purity of Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)is more than 98.15% ,determined by SEC-HPLC.)

SDS-PAGE (Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)

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CDH17/Cadherin-17, Monoclonal Recombinant Antibody (Cat# AAA29399)

Full Name

Anti-CDH17/Cadherin-17 Reference Antibody (10C12)

Gene Names

CDH17; HPT1; CDH16; HPT-1

Reactivity

Human

Applications

Flow Cytometry, Functional Assay

Pricing

$555/0.1 mg $1,045/2x0.1 mg $1,510/3x0.1 mg $1,955/4x0.1 mg $2,145/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded renal cancer tissues using CD203C mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells using CD203C mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western Blot analysis using CD203C mAb against HEK293 (1) and CD203C (AA: extra 45-163)-hIgGFc transfected HEK293 (2) cell lysate)

ELISA (Black Line: Control Antigen (100ng)Purple Line: Antigen (10ng)Blue Line: Antigen (50ng)Red Line: Antigen (100ng))

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CD203C, Monoclonal Antibody (Cat# AAA27937)

Full Name

Mouse Monoclonal Antibody to CD203C

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$415/0.1 mL $735/0.1 mL (AF350) $735/0.1 mL (AF405L) $735/0.1 mL (AF405M) $735/0.1 mL (AF405S) $735/0.1 mL (AF488) $735/0.1 mL (AF546) $735/0.1 mL (AF555) $735/0.1 mL (AF568) $735/0.1 mL (AF594) $735/0.1 mL (AF610) $735/0.1 mL (AF635) $735/0.1 mL (AF647) $735/0.1 mL (AF680) $735/0.1 mL (AF750) $735/0.1 mL (AP) $735/0.1 mL (APC) $735/0.1 mL (Biotin) $735/0.1 mL (Cy3) $735/0.1 mL (Cy5) $735/0.1 mL (Cy5.5) $735/0.1 mL (Cy7) $735/0.1 mL (FITC) $735/0.1 mL (HRP) $735/0.1 mL (PE) $1,415/2x0.1 mL (AF350) $2,050/3x0.1 mL (AF350) $2,655/4x0.1 mL (AF350)

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ICC (Immunocytochemistry) (Confocal imaging of Hep G2 cells using ACSL1 Rabbit PolymAb® (A22737-PM, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human liver tissue using ACSL1 Rabbit PolymAb® (A22737-PM, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse liver tissue using ACSL1 Rabbit PolymAb® (A22737-PM, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat liver tissue using ACSL1 Rabbit PolymAb® (A22737-PM, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using ACSL1 Rabbit PolymAb® (AAA28557) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using ACSL1 Rabbit PolymAb® (AAA28557) at 1:15000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

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ACSL1, Monoclonal Antibody (Cat# AAA28557)

Full Name

ACSL1 Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SF2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: K562)

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SF2, Monoclonal Antibody (Cat# AAA30465)

Full Name

SF2 Antibody

Gene Names

SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human heart tissue using MYH6/MYH7 Rabbit PolymAb® (A26491-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse heart tissue using MYH6/MYH7 Rabbit PolymAb® (A26491-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat heart tissue using MYH6/MYH7 Rabbit PolymAb® (A26491-PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle tissue using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human heart tissue using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Rat heart using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of lysates from 293T cells using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MYH6/MYH7 using MYH6/MYH7 Rabbit PolymAb® (AAA28584) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

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MYH6/MYH7, Monoclonal Antibody (Cat# AAA28584)

Full Name

MYH6/MYH7 Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human colon tissue using CDK1/2 Rabbit PolymAb® (AAA28591, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using CDK1/2 Rabbit PolymAb® (AAA28591) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using CDK1/2 Rabbit PolymAb® (AAA28591) at 1:3000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)

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CDK1/2, Monoclonal Antibody (Cat# AAA28591)

Full Name

CDK1/2 Rabbit PolymAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using JAB1/CSN5/COPS5 antibody (AAA28603) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using JAB1/CSN5/COPS5 Rabbit mAb (AAA28603) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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JAB1/CSN5/COPS5, Monoclonal Antibody (Cat# AAA28603)

Full Name

JAB1/CSN5/COPS5 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using FGFR3 Rabbit mAb (AAA28474) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human testis tissue using FGFR3 Rabbit mAb (AAA28474) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using FGFR3 Rabbit mAb (AAA28474) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using FGFR3 Rabbit mAb (AAA28474) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using FGFR3 Rabbit mAb (AAA28474) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using FGFR3 Rabbit mAb (AAA28474) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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FGFR3, Monoclonal Antibody (Cat# AAA28474)

Full Name

FGFR3 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat brain tissue using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse brain tissue using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human brain tissue using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of C6 cells using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of Neuro-2a cells using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of SH-SY5Y cells using PGP9.5/UCHL1 Rabbit mAb (AAA28485, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using PGP9.5/UCHL1 Rabbit mAb (AAA28485) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas using PGP9.5/UCHL1 Rabbit mAb (AAA28485) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using PGP9.5/UCHL1 Rabbit mAb (AAA28485) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using PGP9.5/UCHL1 Rabbit mAb (AAA28485) at 1:120000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): K-562Exposure time: 30s.)

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PGP9.5/UCHL1, Monoclonal Antibody (Cat# AAA28485)

Full Name

PGP9.5/UCHL1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded mouse brain tissue using S100B Rabbit mAb (AAA28487, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded rat brain tissue using S100B Rabbit mAb (AAA28487, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using S100B Rabbit mAb (AAA28487) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from A-375 cells using S100B Rabbit mAb (AAA28487) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of various lysates using S100B Rabbit mAb (AAA28487) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)

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S100B, Monoclonal Antibody (Cat# AAA28487)

Full Name

S100B Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 8A3 depletes VPS35 from the A549 cell extract..)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

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VPS35, Monoclonal Antibody (Cat# AAA27683)

Full Name

VPS35 Antibody, Clone 8A3: ATTO 390

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

$680/0.1 mg $1,300/2x0.1 mg $1,890/3x0.1 mg $2,440/4x0.1 mg $2,670/5x0.1 mg

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

WB (Western Blot) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)

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VPS35, Monoclonal Antibody (Cat# AAA27702)

Full Name

VPS35 Antibody, Clone 11H10: ATTO 488

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

$680/0.1 mg $1,300/2x0.1 mg $1,890/3x0.1 mg $2,440/4x0.1 mg $2,665/5x0.1 mg

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

Application Data (Zhang. Yanqiong. et al. "A discovery of clinically approved formula FBRP for repositioning to treat HCC by inhibiting PI3K/AKT/NF-?B activation." Molecular Therapy-Nucleic Acids19 (2020): 890-904.)

WB (Western Blot) (Western blot analysis of lysates from 1) COS7.2) 3T3.3) Hela cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot) (Western blot analysis of Hela. diluted at 1) 1:2000 2) 1:5000)

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COX IV, Monoclonal Antibody (Cat# AAA30797)

Full Name

COX IV Monoclonal Antibody (6C8)

Gene Names

COX4I1; COX4; COXIV; COX4-1

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$320/0.1 mL $870/0.5 mL $1,380/1 mL $2,665/2x1 mL $3,875/3x1 mL $5,020/4x1 mL $5,865/5x1 mL

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Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)

Application Data (Published customer image: Blockade of the mevalonate pathway increases CD9 and CD81. (A) RAW264.7 cells were untreated (-) or treated for 48 h with 50 ng/ml TSA (+) in the absence (-) or presence of 50 uM theophylline or 0.5 uM fluvastatin (Fluv) (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) The mevalonate pathway and inhibitors. n-BP, nitrogenous bisphosphonate. (C) RAW264.7 cells were cultured for 24 h in the presence of indicated concentrations of fluvastatin, simvastatin (Simv), zoledronate (Zol), or risedronate (Ris). Levels of CD9 and CD81 were examined by immunoblotting. (D) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) or presence of mevalonate (Mev), farnesyl pyrophosphate (FPP), squalene (Squ), or geranylgeranyl pyrophosphate (GGPP). Although the actin level in the GGPP lane appears to be lower, an equal amount of protein was loaded. (E) RAW264.7 cells were cultured for 24 h in the absence (V) or presence of fluvastatin, zoledronate, farnesyl transferase inhibitor (FTI), or geranylgeranyl transferase inhibitor (GGTI). (F) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (G) RAW264.7 cells were untreated (-) or treated with zoledronate (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (H) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP and stimulated for 15 min with 0.1 ug/ml LPS (+). The cells were lysed, and levels of I?Ba were examined by immunoblotting. (I) RAW264.7 cells were cultured for 24 h in the indicated concentrations of HA1077. Levels of CD9 and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Screening of a drug library for agents that upregulate CD9 or CD81 in RAW264.7 macrophages. (A) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) and presence of each drug (10 uM). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Blots of results with fluvastatin (Fluv) and simvastatin (Simv) are shown. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) After testing 1,165 drugs, levels of CD9 and CD81 relative to actin were quantified by densitometry. Fold changes of the expression levels compared with vehicle alone were calculated and plotted. Drugs that increased the level of either CD9 or CD81 more than 1.5-fold compared with vehicle alone were regarded as positive. Correlation between fold changes in CD9 and CD81 levels was analyzed using Pearson's correlation coefficient. (C) RAW264.7 cells were cultured in the absence (V) or presence of multiple statins (10 uM) and levels of CD9 and CD81 were examined by immunoblotting. The statins are arranged in order of decreasing lipophilicity. Ceri, cerivastatin; Simv, simvastatin; Fluv, fluvastatin; Ator, atorvastatin; Rosu, rosuvastatin; Prav, pravastatin. (D) RAW264.7 cells were cultured in the absence (shaded histograms) or presence (10 uM) of fluvastatin (open red histograms) and simvastatin (open blue histograms). Surface levels of CD9, CD63, CD81, and the integrin beta1 subunit were analyzed by flow cytometry.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Density fractionation of EVs. The figure shows sucrose gradients of EVs preparations from MLP29 (A) and RH (B). Aliquots of these fractions were used for RNA extraction and protein extraction; the most abundant transcripts were found in the fractions containing typical exosomal markers (Tsg101 or Aip1). RH preparations showed more diversity, with vesicle populations fractionating at different densities.From: Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, et al. (2013) Transcriptome of Extracellular Vesicles Released by Hepatocytes. PLoS ONE 8(7): e68693.)

Application Data (Published customer image: The anti-inflammatory effects of statins are CD9-dependent. (A) BMDMs from WT mice were cultured for 24 h in the absence (-) or presence of 3 uM fluvastatin (Fluv) (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) BMDMs from WT and CD9 KO mice were cultured in the absence or presence of the indicated concentrations of fluvastatin, and stimulated for 18 h with 10 ug/ml LPS (+). Activities of MMP-9 in culture supernatants were analyzed by gelatin zymography. (C) BMDMs from WT and CD9 KO mice were cultured in the absence (vehicle) or presence of 10 uM fluvastatin or simvastatin (Simv), and unstimulated (-) or stimulated for 18 h with 1 ug/ml LPS (+). Concentrations of TNF-a in culture supernatants were measured by ELISA. Each bar represents the mean +/- SEM. ±P < 0.05; * * P < 0.01. From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Statins transfer CD14 from lipid rafts into CD9-enriched microdomains. (A) RAW264.7 cells were stimulated with 0.1 ug/ml LPS and, after the indicated times, the cells were lysed and protein levels were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured for 24 h in the absence (-) or presence of 5 uM fluvastatin (Fluv) or simvastatin (Simv) (+) and stimulated for 2 h with 1 ug/ml LPS (+). Proteins in whole-cell lysate (WCL) and CD14 protein in immunoprecipitates (IP) with anti-TLR4 Ab were immunoblotted (IB). (C) RAW264.7 cells were treated as in B. Lysates of untreated (C, control) cultures or LPS-stimulated cultures in the absence (L) or presence of fluvastatin (FL) or simvastatin (SL) were fractionated by sucrose density gradients, and protein distributions were visualized by immunoblotting. The intensities of blots were quantified by densitometry, and percentages of density units of light membrane (LM) fractions are displayed to the right of the blots. Data shown are from one representative of three similar experiments. (D) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb. (E) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb from pooled LM fractions (4 and 5) and dense (D) fractions (9 and 10). In the presence of statins, more CD14/CD9 complexes were formed in dense fractions (arrowheads).From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Fluvastatin and simvastatin increase CD9 and CD81 levels in RAW264.7 cells.(A) RAW264.7 cells were cultured for 24 h in the absence or presence of increasing concentrations of fluvastatin (Fluv) or simvastatin (Simv). The cells were lysed, and levels of CD9, CD63, and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured in the absence or presence of increasing concentrations of fluvastatin or simvastatin and stimulated for 24 h with 0.1 ug/ml LPS (+). Levels of CD9, CD63, and CD81 were examined by immunoblotting. Note that LPS downregulates CD9 and CD81 in the absence of statins (arrowheads). (C) RAW264.7 cells were cultured in the absence (-) or presence of 3 uM fluvastatin (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). mRNA levels of CD9 and CD81 were examined by reverse transcription PCR. GAPDH is an internal loading control. (D) RAW264.7 cells were cultured in the absence or presence of fluvastatin, and unstimulated or stimulated with LPS. Control (Cont) was an untreated culture. mRNA levels of CD9 and CD81 were examined by real-time PCR. Data shown are from one representative of three similar experiments. (E) Human monocytic THP-1 cells were treated for 4 h with 1 ug/ml phorbol 12-myristate 13-acetate, allowed to attach to a plate, and then cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting. (F) Mouse 3T3 fibroblasts were cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Staining of mouse spleen cells with Hamster anti Mouse CD81:FITC)

Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 647)

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CD81, Monoclonal Antibody (Cat# AAA11942)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Reactivity

Rat

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged Rho GTPase-activating protein 28 protein by western blotting and immunofluorescenceImage caption:Arhgap28-V5 inhibits RhoA activation and stress fiber formation in SaOS-2 cells. SaOS-2 cells were transfected with empty vector or Arhgap28-V5. A. The expression of Arhgap28-V5 was confirmed by western blotting using an antibody to V5. B. Effect of Arhgap28-V5 expression on the basal activity of RhoA (n = 5), Rac1 (n = 3) and Cdc42 (n = 3). Bars show SEM. * indicates significant difference found, p)

Application Data (Published customer image: Mouse anti V5 Tag antibody, clone SV5-Pk1used for immunoprecipitation of V5 tagged RNF11 proteinImage caption:Dynamic associations of RNF11 with both A20 and Itch in primary neurons. N2A cells transduced with V5-RNF11 lentivirus (N2A V5-RNF11) and transfected with DYKDDDDK-A20 were harvested for immunoprecipitation (IP) with V5 antibody (A) or harvested for IP with DYKDDDDK antibody (B). Proteins were resolved by SDS-PAGE and immunoblotted with anti-A20 and RNF11. In parallel, pull-down assays with V5 antibody (C) or with Itch antibody (D) from N2A V5-RNF11 cell lysates were resolved by SDS-PAGE. Immunoprecipitates and lysates were immunoblotted with anti-Itch and RNF11. (E) and (H) Murine primary cortical neurons were stimulated with 10?ng/ml TNF-a for 0 or 30 minutes and harvested for IP with RNF11 antibody. Control IP experiments were performed with antibody omitted. Proteins were resolved by SDS-PAGE and immunoblotted with anti-A20, Itch, RNF11 and actin. (F), (G), (I) and (J) ImageJ software was used to quantify the densitometry of the immunoprecipitated bands relative to the 0-minutes time point. Each input sample's immunoreactivity was used as a loading control. All IPs are representative of at least three independent experiments. *P?)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged podocin protein by western blottingImage caption:The podocin short isoform is N-glycosylated. A) PNGase-F treatment removes the double band from the short isoform in DRM fraction 7. Lysates from Figure 3 were subjected to treatment with PNGase-F and immunoblotted and detected with anti-V5 antibody. B) N to S mutation of the N-glycosylation consensus motif completely abrogates the formation of a double band. The asparagine at position 287 corresponds to amino acid 355 in the full-length protein. HEK293T cells were transfected with V5-tagged Podocin (short isoform or short isoform N287S, respectively) and lysates were immunoblotted and detected with anti-V5 antibody.From: V¶lker LA, Schurek EM, Rinschen MM, Tax J, Schutte BA, Lamkemeyer T, Ungrue D, Schermer B, Benzing T, H¶hne M. Characterization of a short isoform of the kidney protein podocin in human kidney. BMC Nephrol. 2013 May 6;14:102.)

Application Data (V5 tagged protein detected with Mouse anti V5-Tag)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged podocin protein by western blottingImage caption:The podocin short isoform interacts with known podocyte proteins. CD2AP, TRPC6, neprin and NEPH1 co-precipitate with both podocin isoforms (FL, full length (canoncical isoform); SI, short isoform). DYKDDDDK- and V5-tagged proteins were expressed in HEK293T cells and precipitated with anti-DYKDDDDK antibody as indicated. Western blot analysis was performed with a V5 specific antibody. Expression levels of DYKDDDDK.podocin constructs in the lysates are shown below.From: V¶lker LA, Schurek EM, Rinschen MM, Tax J, Schutte BA, Lamkemeyer T, Ungrue D, Schermer B, Benzing T, H¶hne M. Characterization of a short isoform of the kidney protein podocin in human kidney. BMC Nephrol. 2013 May 6;14:102.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not regulated by Cullin E3 Ubiquitin ligases. (A,B) Untransfected (A) or REDD1-V5 pcDNA3 (0.3 ug) transfected (B) HEK293 cells stably expressing tetracycline inducible dnUbc12-HA were induced with 1 ug/ml tetracycline for 24 hours (A,B) or treated with 3 uM MLN4924 (A) or 20 uM MG-132 (A,B) for 8 hours followed by cell lysis. (C,D) Untransfected HEK293 (C) or HEK293 transfected with REDD1-V5 pcDNA3 (0.3 ug) (D) were pre-treated with 3 uM MLN4924 followed by cycloheximide (40 uM) treatment and cell lysis at the indicated time points. (E) HEK293 cells stably expressing tetracycline inducible dnUbc12-HA were induced with 1 ug/ml tetracycline for 24 hours followed by cycloheximide (40 uM) treatment and cell lysis at the indicated time points.From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged HIVA peptide by western blottingImage caption:Construction of the BCG.HIVACAT vaccine strain. (A) A synthetic GC-rich HIVA gene was fused to the region encoding the 19-kDa lipoprotein signal sequence and inserted into the episomal pJH222 E Coli-mycobacterium shuttle plasmid. This plasmid contains kanamycin resistance (aph) and complementing lysA genes and an E Coli origin of replication (oriE). In addition, pJH222 contained the mycobacterial origin of replication (oriM). The BALB/c mouse T-cell and MAb Pk epitopes used in this work are depicted. P a-Ag, M. tuberculosis a-antigen promoter; PHSP60, heat shock protein 60 gene promoter. The aph gene was removed by SpeI digestion and the lacO sequence was inserted and transformed into E Coli DH1lacdapD strain. (B) Immunodot of BCG.HIVACAT lysates. Dot 1: BCG wild type (negative control); Dot 2, 3, 4 and 5: clone 3, clone 7, clone 9 and clone 10 of BCG.HIVACAT; Dot 6: BCG.HIVA222 (positive control). HIVA peptide was detected using the anti-Pk MAb followed by horseradish peroxidase-Goat-anti-Mouse and enhanced chemiluminescence (ECL) detection. (C) In vivo plasmid stability of BCG.HIVACAT harboring pJH222.HIVACAT. Mice were injected s.c. with 105 cfu of BCG.HIVACAT and boosted i.m. with 106 pfu of MVA.HIVA, spleens were homogenized 20 weeks after BCG inoculation and the recovered rBCG colonies were tested for the presence of the HIVA DNA coding sequence by PCR. Lanes 1 to 6: Six rBCG colonies were recovered in the non-lysine supplemented plate; lane 7: Molecular weight marker; lane 8: Plasmid DNA positive control; lane 9: Distilled water (negative control).From: Saubi N, Mbewe-Mvula A, Gea-Mallorqui E, Rosario M, Gatell JM, et al. (2012) Pre-Clinical Development of BCG.HIVACAT, an Antibiotic-Free Selection Strain, for HIV-TB Pediatric Vaccine Vectored by Lysine Auxotroph of BCG. PLoS ONE 7(8): e42559.)

Application Data (Published customer image: Mouse anti V5 Tag antibody, clone SV5-Pk1used for immunoprecipitation of V5 tagged RNF11 proteinImage caption:Myristoylation mutant of RNF11 is unable to associate with Itch. SH-SY5Y shRNA-RNF11 cells were transfected with shRNA-resistant RNF11 constructs or vector. Coimmunoprecipitation experiments using V5 antibody were performed 24 hours after transfection. Immunoprecipitates and lysates were resolved by SDS-PAGE and immunoblotted with anti-A20, Itch, RNF11 or actin. Blots are representative of three independent experiments. From: Pranski EL, Dalal NV, Herskowitz JH, Orr AL, Roesch LA, Fritz JJ, Heilman C, Lah JJ, Levey AI, Betarbet RS. Neuronal RING finger protein 11 (RNF11) regulates canonical NF-?B signaling. J Neuroinflammation. 2012 Apr 16;9:67. doi: 10.1186/1742-2094-9-67.)

Application Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

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V5-TAG, Monoclonal Antibody (Cat# AAA12211)

Full Name

MOUSE ANTI V5-TAG

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay

Pricing

$1,635/2 mg $3,160/2x2 mg $4,600/3x2 mg $5,960/4x2 mg $7,020/5x2 mg

FCM (Flow Cytometry) (Figure-1: Cell surface flowcytometry analysis of CLEC1on human PBMC (Monocytes) using 0.5 ug/10^6 Cells of Anti-CLEC1 antibody. Green represent isotype control and red represent Anti human CLEC1 antibody. Goat anti mouse PE conjugated was used as the secondary antibody.)

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CLEC1, Monoclonal Antibody (Cat# AAA14882)

Full Name

Monoclonal Antibody to CLEC1 (Clone: ABM2H82)

Gene Names

CLEC1A; CLEC1; CLEC-1

Reactivity

Human

Applications

Flow Cytometry

Purity

Protein G Chromatography Purified

Pricing

$240/0.025 mg $420/0.1 mg $795/2x0.1 mg $1,145/3x0.1 mg $1,475/4x0.1 mg $1,635/5x0.1 mg

Application Data

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HLA Class 1 Antigen A33,B8, Monoclonal Antibody (Cat# AAA14684)

Full Name

Mouse anti-Human HLA Class 1 Antigen A33,B8

Reactivity

Human

Applications

Cell Typing

Purity

Ascites

Pricing

$1,600/0.1 mL $3,085/2x0.1 mL $4,495/3x0.1 mL $5,825/4x0.1 mL $6,895/5x0.1 mL

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IHC (Immunohistchemistry) (Figure. DAB staining on IHC-P; Samples: Mouse Liver Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Lung Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Mouse Liver Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant IL1F9, Mouse.)

WB (Western Blot) (Western Blot: Sample: Rat SerumPrimary Ab: 3ug/ml Mouse Anti-MouseIL1F9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Interleukin 1 Family, Monoclonal Antibody (Cat# AAA21005)

Full Name

Interleukin 1 Family, Member 9 (IL1F9) Monoclonal Antibody

Gene Names

Il1f9; Il36g

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

$270/0.1 mL $330/0.2 mL $545/0.5 mL $645/1 mL $1,740/5 mL $3,370/2x5 mL $4,910/3x5 mL $6,365/4x5 mL $7,755/5x5 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat spleen tissue using CD8A Rabbit PolymAb® (AAA28561, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse spleen tissue using CD8A Rabbit PolymAb® (AAA28561, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human colon tissue using CD8A Rabbit PolymAb® (AAA28561, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human breast cancer tissue using CD8A Rabbit PolymAb® (AAA28561, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CD8A Rabbit PolymAb® (AAA28561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD8A Rabbit PolymAb® (AAA28561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD8A Rabbit PolymAb® (AAA28561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD8A Rabbit PolymAb® (AAA28561) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD8A Rabbit PolymAb® (AAA28561) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using CD8A Rabbit PolymAb® (AAA28561) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): Mouse brainExposure time: 10s.)

WB (Western Blot) (Western blot analysis of lysates from Mouse spleen, using CD8A Rabbit PolymAb® (AAA28561) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)

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CD8A, Monoclonal Antibody (Cat# AAA28561)

Full Name

CD8A Rabbit PolymAb

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast-cancer tissue. 1.HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.AIM2 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HSP70 Monoclonal Antibody(3G10)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse mAb diluted at 1:500.)

Application Data (Niu. Ziru. et al. "Polymer-based precipitation preserves biological activities of extracellular vesicles from an endometrial cell line." PloS one 12.10 (2017): e0186534.)

WB (Western Blot) (Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2.000.)

WB (Western Blot) (Western blot analysis of lysates from HT-29. NIH/3T3. and HepG2 cells. primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot) (Western blot analysis of 1) Hela. 2) Mouse Brain. diluted at 1:2000.)

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HSP70, Monoclonal Antibody (Cat# AAA30801)

Full Name

HSP70 Monoclonal Antibody (3G10)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$300/0.04 mL $390/0.1 mL $555/0.2 mL $1,045/2x0.2 mL $1,510/3x0.2 mL $1,955/4x0.2 mL $2,150/5x0.2 mL

WB (Western Blot) (Western blot analysis of C3 expression in HepG2 cell lysate (AAA11693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (stacking gel) / 90V (resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 monoclonal antibody (AAA11693) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (please inquire) with Tanon 5200 system. A specific band was detected for C3.)

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C3/Complement C3, Monoclonal Antibody (Cat# AAA11693)

Full Name

Anti-C3/Complement C3 Rabbit Monoclonal Antibody

Gene Names

C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$240/0.03 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,030/5x0.1 mL

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Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105:FITC)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647)

Application Data (Staining of KG1 cells with Mouse anti Human CD105)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488)

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CD105, Monoclonal Antibody (Cat# AAA12083)

Full Name

MOUSE ANTI HUMAN CD105:FITC

Gene Names

ENG; END; HHT1; ORW1

Applications

Flow Cytometry

Pricing

$275/0.025 mg $505/2x0.025 mg $725/3x0.025 mg $920/5x0.025 mg $935/4x0.025 mg

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Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105:FITC)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647)

Application Data (Staining of KG1 cells with Mouse anti Human CD105)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488)

Compare (5 max)

CD105, Monoclonal Antibody (Cat# AAA11865)

Full Name

MOUSE ANTI HUMAN CD105:FITC

Gene Names

ENG; END; HHT1; ORW1

Applications

Flow Cytometry

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

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Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105:FITC)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of KG1 cells with Mouse anti Human CD105RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647)

Application Data (Staining of KG1 cells with Mouse anti Human CD105)

Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488)

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CD105, Monoclonal Antibody (Cat# AAA12029)

Full Name

MOUSE ANTI HUMAN CD105:RPE

Gene Names

ENG; END; HHT1; ORW1

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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FCM (Flow Cytometry) (Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of RIG I  in normal human prostate tissue using RIG I  antibody (Clone: ABM40B5) at 5 ug/ml.)

WB (Western Blot) (Expression analysis of RIGI. Anti- RIGI antibody (Clone:ABM40B5) was used at 2 µg/ml on K562 Lysates.)

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RIG-I, Monoclonal Antibody (Cat# AAA14865)

Full Name

RIG-I Monoclonal Antibody

Gene Names

DDX58; RIGI; RIG-I; RLR-1; SGMRT2

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Western Blot

Purity

Protein G Chromatography

Pricing

$240/0.025 mg $420/0.1 mg $795/2x0.1 mg $1,145/3x0.1 mg $1,475/4x0.1 mg $1,635/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Y-79.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in human ovarian cancer.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Hela S3 NE.)

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MCM3, Monoclonal Antibody (Cat# AAA26113)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (APC)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (human kidney tissue were subjected to SDS PAGE followed by western blot with AAA27494 (TPSAB1 Antibody) at dilution of 1:1000)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA27494 (TPSAB1 Antibody) at dilution of 1:500. Heat mediated antigen retrieved with Citric acid buffer, pH6.0)

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TPSAB1, Monoclonal Antibody (Cat# AAA27494)

Full Name

TPSAB1 Mouse Monoclonal

Gene Names

TPSAB1; TPS1; TPS2; TPSB1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

>95% as determined by SDS-PAGE
Protein A+G purification

Pricing

$415/0.1 mg $785/2x0.1 mg $1,130/3x0.1 mg $1,455/4x0.1 mg $1,550/5x0.1 mg

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)

Application Data

WB (Western Blot) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)

IP (Immunoprecipitation) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)

IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 10A8 depletes VPS35 from the A549 cell extract..)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

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VPS35, Monoclonal Antibody (Cat# AAA27697)

Full Name

VPS35 Antibody, Clone 10A8: HRP

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

$665/0.1 mg $1,265/2x0.1 mg $1,845/3x0.1 mg $2,385/4x0.1 mg $2,595/5x0.1 mg

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FCM (Flow Cytometry) (Untransfected CT26 cells (green line) and transfected Human LY6G6D CT26 stable cells (red line) were stained with anti-LY6G6D antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.)

FCM (Flow Cytometry) (Untransfected HEK293T cells (green line) and transfected Human LY6G6D HEK293T stable cells (red line) were stained with anti-LY6G6D antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.)

ELISA (The Binding Activity of Macaca fascicularis LY6G6D with Anti-LY6G6D Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LY6G6D at 2 ?g/mL can bind Anti-LY6G6D recombinant antibody. The EC50 is 16.16-25.29 ng/mL.)

ELISA (The Binding Activity of Mouse Ly6g6d with Anti-LY6G6D Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Ly6g6d at 2 ?g/mL can bind Anti-LY6G6D recombinant antibody. The EC50 is 1.159-2.305 ?g/mL.)

ELISA (The Binding Activity of Human LY6G6D with Anti-LY6G6D Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D at 2 ?g/mL can bind Anti-LY6G6D recombinant antibody. The EC50 is 1.840-2.204 ng/mL.)

ELISA (The Binding Activity of Rat Ly6g6d with Anti-LY6G6D Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Rat Ly6g6d at 5 ?g/mL can bind Anti-LY6G6D recombinant antibody. The EC50 is 4.245-5.843 ?g/mL.)

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LY6G6D, Monoclonal Recombinant Antibody (Cat# AAA28075)

Full Name

LY6G6D Recombinant Monoclonal Antibody

Gene Names

LY6G6D; G6D; NG25; LY6-D; MEGT1; C6orf23

Reactivity

Human

Applications

Flow Cytometry

Purity

Affinity purified

Pricing

$305/0.05 mL $400/0.1 mL $750/2x0.1 mL $1,090/3x0.1 mL $1,400/4x0.1 mL $1,705/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.