Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor(AR-V7 specific) Antibody.)
WB (Western Blot) (Western blot analysis of Androgen Receptor expression in 22Rv1 cell lysate.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry) (Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
WB (Western Blot) (Western blot detection of DNA-PKcs in Hela.Molt-4.A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
WB (Western Blot) (Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PD-1 (ABT-PD1)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PGP9.5 antibody. The HRP-conjugated)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PGP 9.5 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
SDS-PAGE (Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PR(ABT-PR.1)antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using Smad4 Antibody(AAA46863)SMAD4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD4 Antibody (AAA46863)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot) (Western blot analysis of SMAD4 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate (AAA46863).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD4)
WB (Western Blot) (Mouse Anti-Slo2.2 Potassium Channel Antibody [N3/26] used in Western Blot (WB) on Rat brain membrane lysate)
IHC (Immunohistochemisry) (Mouse Anti-Slo2.2 Potassium Channel Antibody [N3/26] used in Immunohistochemistry (IHC) on Mouse backskin)
ICC (Immunocytochemistry) (Mouse Anti-Slo2.2 Potassium Channel Antibody [N3/26] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells)
ICC (Immunocytochemistry) (Mouse Anti-Slo2.2 Potassium Channel Antibody [N3/26] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cells (SH-SY5Y))
WB (Western Blot) (Western Blot analysis of Human,Mouse,Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3F8 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3F8 . Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000,1:3000 for 60 min at RT,5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody,Clone 3F8 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000,1:5000 for 60 min at RT,5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of TOX-3 in human stomach tissue using TOX-3 antibody (Clone: ABM44A1) at 5 ug/ml.)
WB (Western Blot) (Expression analysis of TOX-3. Anti- TOX-3 antibody (Clone: ABM44A1) was tested at 0.5 ug/ml and 2 ug/ml on (1) recombinant protein (2) A549 (3) A431 lysates. respectively)
IHC (Immunohistochemisry) (Immunohistochemical analysis of MGLL protein in paraffin embedded Adenocarcinoma of Human ovary tissue using MGLL antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MGLL protein (Right) or empty vector (Left) detected with MGLL antibody)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant MGLL protein using MGLL antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NRBP1 protein (Right) or empty vector (Left) detected with NRBP1 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NRBP1 protein (red) or empty vector (blue) stained using NRBP1 antibody)
ELISA (Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7367, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot) (Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7367, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
WB (Western Blot) (Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATF4 Antibody (D) Composite.)
WB (Western Blot) (Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.)
WB (Western Blot) (Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATF4 Antibody (D) Composite.)
WB (Western Blot) (Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK9 protein using NEK9 antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK9 protein (Right) or empty vector (Left) detected with NEK9 antibody)
FCM/FACS (Flow Cytometry) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NEK9 protein (red) or empty vector (blue) stained using NEK9 antibody)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BTN3A1 protein, mFc-His tagged protein PME100056 can bind Rabbit anti-BTN3A1 monoclonal antibody ( clone: DM94) in a linear range of 0.64-80 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BTN3A1 protein, mFc-His tagged protein PME100056 can bind Rabbit anti-BTN3A1 monoclonal antibody ( clone: DM94) in a linear range of 0.64-80 ng/ml.)
FCM/FACS (Flow Cytometry) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1 protein, hFc tagged protein PME100473 can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM109) in a linear range of 0.6-80 ng/ml.)
ELISA (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1 protein, hFc tagged protein PME100473 can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM109) in a linear range of 0.6-80 ng/ml.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of FRK protein in paraffin embedded Human endometrium tissue using FRK antibody)
WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FRK protein (Right) or empty vector (Left) detected with FRK antibody)
FCM/FACS (Flow Cytometry) (Flow-cytometry using the Anti-CD131 antibody BION-1 or the rabbit IgG version of BION-1 ( at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence) (Immunofluorescence staining of U937 cells using anti-CD131 antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Purity
Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry) (Immunohistochemistry: Human placenta (FFPE) stained with Mouse anti- Uroplakin-2 (Cat# AAA71363) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HTATIP mAb. [Lot No. 3557C4a-1])
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-HTATIP monoclonal antibody.)
IF (Immunofluorescence) (ImmunofluorescencestainingofRAW264.7cellswithAnti-NKG2D(AAA72534)CX-5 ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsstainedwiththechimericrabbitIgGversionofCX-5(AAA72534)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72534,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemisry) (IHC image diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry) (IHC image diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysateAll lanes: UQCRC2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 kDaObserved band size: 49 kDa)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl P53 (K382) Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Acetyl P53 (K382) Mouse mAb diluted at 1:200.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Phospho-Histone H2A.X (S139) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-Histone H2A.X (S139) on HepG2 cell lysates using anti-Phospho-Histone H2A.X (S139) antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 cell lysate�Ctreated with etoposide Lane 3: HepG2 cell lysate�Cuntreated)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of A431 cells with p63 antibody at 1/50 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining p63 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-p63 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of p63 on human kidney lysates using anti-p63 antibody at 1/1, 000 dilution.)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymus)
FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Hela cells with FBXL10 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
WB (Western Blot) (Western blot analysis of FBXL10 on A549 cell lysate using anti-FBXL10 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of FBXL10 on HEK293 (1) and FBXL10-hIgGFc transfected HEK293 (2) cell lysate using anti-FBXL10 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of FBXL10 on human FBXL10 recombinant protein using anti-FBXL10 antibody at 1/1, 000 dilution.)
Monoclonal Antibody to Plasminogen Activator Inhibitor 1 (PAI1)
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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